• Food Science and Biotechnology
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• 제15권6호
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• pp.889-895
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• 2006

Cytotoxic effects of extracts from Tremella fuciformis strain FB001 were evaluated on the DLD-1 human colon adenocarcinoma cell line and the content of polyphenolic compounds in the extracts were analyzed. Hexane, chloroform, and ethyl acetate subfractions (experimental setting I) exhibited cytotoxic effects on the human colon adenocarcinoma DLD-1 cell line with $IC_{50}$ values of 350, 400, and 450 ppm, respectively. When T. fuciformis was extracted sequentially with ether, ethyl acetate, chloroform, and ethanol (experimental setting II), the ether extract demonstrated potent cytotoxicity with an $IC_{50}$ value of 150 ppm, followed by ethyl acetate and chloroform fractions. If the first extraction solvent was chloroform instead of ether (experimental setting III), exposure of the cell line to chloroform, ethyl acetate, and ether extracts at 1,000 ppm led to cell death. High levels of phenolic compounds were estimated for all hydrophobic extracts, which exhibited cytotoxic effects. We propose that this useful information gives additional support to our understanding of the biology and utility of this particular mushroom.

• 한국식품영양과학회지
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• 제33권4호
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• pp.609-613
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• 2004

본 연구는 정향추출물(물, 메탄올 및 에테르)의 항산화 효과를 리놀레인산 기질 및 리놀레인산 에멀젼기질에서 비교, 검토하였다. 물, 메탄올 및 에테르용매에 따른 정향의 총페놀 함량은 23.1, 42.8, 34.9%로 메탄올＞에테르＞물 추출물 순이었다. Eugenol 함량은 메탄올 추출물의 경우 6.1%이었고 에테르 추출물 8.1%이었으며 물 추출물에서는 검출되지 않았다. 정향 추출물의 DPPH 소거효과는 물 추출물 경우 36.1%, 메탄을 추출물의 경우 30.9%, 에테르 추출물의 경우 29.7%로 나타났다. 리놀레인산 기질에서의 항산화 효과는 BHT＞에테르 추출물＞ $\alpha$-tocopherol＞메탄올 추출물＞물 추출물의 순이었다. 리놀레인산 에멀젼 시스템에서의 항산화 효과는 BHT＞물 추출물＞메탄올 추출물＞에테르 추출물＞ $\alpha$-tocopherol의 순이었다.

• Journal of Nutrition and Health
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• 제38권2호
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• pp.112-116
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• 2005

본 연구에서는 식중독 유발세균에 대한 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 예로부터 민간과 한방에서 널리 이용되어 온 선학초를 각종 유기용매로 추출하여 식중독 유발세균에 대한 항균활성을 조사해 보았다. 선학초를 methanol로 추출한 후, Petroleum ether, chloroform, ethyl acetate를 이용하여 실온에서 각각 용매별로 계통 분획하고, 수용성추출물을 얻은 후, 9종의 식중독 유발세균 (Staphylococcus Aureus, Bacillus Cereus, Salmonella Enteritidis, Shigella Flexneri, Escherichia Coli, Salmonella Typhimurium, Shigella Dysenteriae, Pseudomonas Aruginosa, Shigella Sonnei)에 대하여 항균효과를 조사하였다. 선학초 추출물의 농도별 항균 활성 검색에서는 선학초의 petroleum ehter 추출물이 가장 큰 항균 효과를 보였으며 Pseudomonas Aruginosa가 가장 민감하게 반응하는 균주였다. 선학초의 petroleum ehter 추출물과 자소엽의 methanol 추출물을 혼합하여 항균력을 측정해 본 결과 두 추출물을 섞어 첨가했을 경우가 단독으로 사용했을 때 보다 상승효과를 나타내었다. 또한 선학초의 petroleum ehter 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 Bacillus Cereus와 Salmonella Enteritidis 배양액에 선학초의 petroleum ehter 추출물을 각각 4,000 ppm 농도로 첨가했을 때, Bacillus Cereus의 생육이 24시간 이상까지 억제됨을 관찰할 수 있었고, Salmonella Enteritidis 의 생육도 36시간까지 지연시킬 수 있었다. 이상의 결과 선학초의 petroleum ehter 추출물은 Bacillus Cereus와 Salmonella Enteritidis의 성장을 억제하는 것을 알 수 있었다. 이상의 결과 선학초의 petroleum ether 추출물은 각종 식중독 유발세균의 성장을 억제하는 천연 식품보존제 개발의 신소재로 활용될 가능성이 있음을 알 수 있었다.

• 생약학회지
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• 제28권4호
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• pp.192-197
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• 1997

As a part of searching for new antitumor agents from natural products, 94 kinds of Chinese plants were extracted with petroleum ether/ether (1:1), ethyl acetate and methanol, successively and their cytotoxicities were evaluated against A549 (human lung carcinoma) cell line. Among them, six kinds of ether extracts, seven kinds of ethyl acetate extracts and one kind of methanol extracts showed significant cytotoxic activities (above 70% inhibition) at a concentration of $50\;{\mu}g/ml$. These results surest that they may be involved in natural sources with possible anticancer activities.

• Journal of Ginseng Research
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• 제18권2호
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• pp.108-112
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• 1994

Rats (Sprague Dawley, male, 200 g) were fed with 15% corn oil containing a large quantity of 18 2 (linoleic acid) for 3 weeks, and were followed by feeding the petroleum ether extracts from Korean red ginseng for 3 weeks. cGMP was produced more in platelets prepared from both 15% corn oil and petroleum ether extracts-fed group than in platelets only 15% corn oil-fed group, indicating that the production of cGMP is increased by feeding the petroleum ether extracts. When this platelet was stimulated by phorbol-12-myristate-13-acetate (PMA), the level of cGMP was decreased. However, the platelets in medium containing protein fraction (200 $\mu\textrm{g}$/ml) was stimulated by PMA, the production of cGMP inhibited by PMA was increased by 3 times or more. These results suggest that both the protein fraction and the petroleum ether extracts from Korean red ginseng are synergistic in the productiorl of cGMP, and they may have the antiplatelet effects.

• Parasites, Hosts and Diseases
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• 제47권4호
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• pp.377-380
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• 2009

Growing patterns of pediculocidal drug resistance towards head louse laid the foundation for research in exploring novel anti-lice agents from medicinal plants. In the present study, various extracts of Pongamia pinnata leaves were tested against the head louse Pediculus humanus capitis. A filter paper diffusion method was conducted for determining the potential pediculocidal and ovicidal activity of chloroform, petroleum ether, methanol, and water extracts of P. pinnata leaves. The findings revealed that petroleum ether extracts possess excellent anti-lice activity with values ranging between 50.3% and 100% where as chloroform and methanol extracts showed moderate pediculocidal effects. The chloroform and methanol extracts were also successful in inhibiting nymph emergence and the petroleum ether extract was the most effective with a complete inhibition of emergence. Water extract was devoid of both pediculocidal and ovicidal activities. All the results were well comparable with benzoyl benzoate (25% w/v). These results showed the prospect of using P. pinnata leave extracts against P. humanus capitis in difficult situations of emergence of resistance to synthetic anti-lice agents.

• Advances in Traditional Medicine
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• 제9권2호
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• pp.142-148
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• 2009

Anxiety disorders are one of the serious problems which need proper therapy devoid of side effects of presently available medicines. The present study evaluates the anxiolytic and sedative activity of leaf galls of Piper nigrum Linn. in Swiss Albino mice. The pet. ether, chloroform, ethyl acetate and ethanol extracts of leaf galls of Piper nigrum Linn were obtained by continuous soxhlet extraction. The prepared extracts were found to be safe up to 2000 mg/kg body weight of mice in the acute toxicity study. Each extract was assessed for anxiolytic activity in Swiss Albino mice by elevated plus Maze, open field test, rota rod test and phenobarbitone induced sleeping time test. In the Elevated Plus Maze test, the pet.ether extract and chloroform extract at a dose of 50 mg/kg b.w. orally, significantly (P < 0.01) increased the number of entries and time spent in open arm comparable with standard diazepam at the dose of 10 mg/kg. b.w. p.o. In the open field test, pet. ether extract (50 mg/kg b.w. p.o.) showed significant increase (P < 0.01) in ambulation and activity in the center. Chloroform extract (50 mg/kg b.w p.o.) was significant (P < 0.05) for both ambulation and center activity. Pet. ether extract (50 mg/kg b.w. p.o) also showed significant activity (P < 0.01) in rota rod test. All the results are comparable with standard diazepam at the dose of 1 mg /kg b.w, p.o. Moreover all the extracts showed significant (P < 0.01) increase in the phenobarbitone induced sleeping time among which pet.ether showed more prominent activity (36%) comparable with control. The results revealed that, the active pet.ether extract and chloroform extract of leaf galls of Piper nigrum Linn is worthwhile to develop the bioactive principle for anxiolytic activity.

• KSBB Journal
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• 제15권2호
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• pp.162-166
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• 2000

도라지에 H2O, ethanol, ethyl ether와 petroleum ether를 용매로 하여 추출한 시료를 기관지질환 유발세균(S. aureus, C. diphtheriae, Mycobacterium sp. F. nucleatum, A. funicatus, K. pneumoniae, S. pyogenes N. gonorrhoeae)에 투여한 결과 ethyl ether, petroleum ether, H2O와 ethanol의 순으로 항균력이 우수하다는 것이 판명되었다. 특히, ethyl ether에 의한 도라지 추출물의 항균력이 80%이상 뛰어난 효과를 나타내었다. 우수한 항균력을 가지는 도라지 추출물의 농도는 30mg부터 30%이상의 억제작용을 보였으며 70mg에서 70%이상의 세포 성장이 저해되는 현상을 관찰하였다. 기관지 질환 유발세균에 대한 도라지의 효과는 대조구에 비해 70%이상의 탁월한 항균효과가 있는 것으로 확인되었다.

• 생약학회지
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• 제37권3호
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• pp.130-135
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• 2006

The present study describes the preliminary evaluation of the cytotoxic activity of the extracts from Inula japonica. I. japonica was extracted with methanol, ethanol, acetone, and water, and then cytotoxic activity of these extracts were evaluated. The cytotoxic activity of each extract was assessed by the MTT-dye reduction assay. Both ethanol and acetone extracts from I. japonica showed the cytotoxic activity against the HT-29 human colon cancer cells. Furthermore, the ethanol extract was fractionated with n-hexane, diethyl ether, ethyl acetate, and water according to degree of Polarity, The diethyl ether fraction showed the highest cytotoxic activity against HT-29 cells, but the other fractions showed low cytotokic activity. In addition, diethyl ether layer also showed the cytotoxic activity against various tumor cells, such as human colon carcinoma SW620, human cervix adenocarcinoma HeLa, and human breast adenocarcinoma MCF-7 cells as well as HT-29 cells. These studies support that extracts of I. japonica may be a potential candidate as possible chemotherapeutic agent against human cancer.

• 한국식품영양학회지
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• 제11권4호
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• pp.426-430
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• 1998

This study was conducted to investigate electron donating ability, antioxdative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, butanol, petroleum ether and hexane extracts were 194.20$\mu\textrm{g}$/ml, 98.80$\mu\textrm{g}$/ml, 1.60$\mu\textrm{g}$/ml and 0.20$\mu\textrm{g}$/ml, respectively. Electron donating abilities of diethyleter and butanol extracts from Gyrophora esculaenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethyleter and butanol extracts sharply increaed at initial stage. Antioxidative activities of fiethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low s compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum ether from Gyrophora esculenta were 96.07%, 77.39% and 15.07%, respectively.