• Title/Summary/Keyword: esterase isozyme

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Esterase Isozyme Banding Pattern in Leaf and Stem of Legume Plants (콩과식물의 잎과 줄기의 Esterase Isozyme Banding Pattern에 관한 연구)

  • 이성규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.11 no.4
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    • pp.199-202
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    • 1991
  • The esterase isozyme of several legume plants were separated and visualized by horizontal starch gel electrophoresis using enzyme-specific staining. Extracts used were prepared from fully expanded young leaf and stem of six legume species which were red clover(Trifolium Pretense L.), ladino clover(Trifolium repense L.), wild white clover(Trifolium repense L.), alfalfa(Medicage sativa L.), mimosoides(Cassia mimosoides var nomame Makino), and amoena(Vicia amoena Fisch). The number of band, Phenotype and staining intensity of esterase isozyme in leaf and stem varies depending on the plant species. However, there are little difference between leaf and stem esterase isozyme in same species except alfalfa. And in the leaf and stem of mimosoides and amoena showed not any esterase(Fig. 2). Among the examined plants, the highest staining intensity and the rapidest migrating esterase isozyme was Est 1.

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Studies on the Alcohol Dehydrogenase ( ADH ) and Esterase Isozyme banding Pattern of Italian Ryegrass Varieties (이탈리안 라이그라스의 품종별 Alcohol Dehydrogenase ( ADH ) 와 Esterase의 Isozyme Banding Pattern에 관한)

  • 이성규;박병훈
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.14 no.2
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    • pp.82-87
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    • 1994
  • This study was planned to identify the variety of Italian ryegrass using electrophoresis. Thirty seven varieties of Italian ryegrass were tested by starch gel electrophoresis. The specific electrophoretic zymograms of each variety were observed by Alcohol dehydrogenase(ADH) and Esterase. The results were surnrnerized as follows; 1. AU varieties displayed two band zones by ADH and Rf values were 0.63 and 0.6 (Table 2, Fig. 2). 2. There were five band type for ADH isozyme of 37 varieties classified with isozyme banding pattern. According to the isozyme band type 7, 2, 6, 18 and 4 varieties belong to group, I, II, III, IV, and V, respectively (Table 2). 3. The varieties displayed single band zone for Esterase isozyme and Rf value was 1.00 (Table 2, Fig. 4). 4. According to banding type, Esterase isozyme of 37 varieties classified into 3 groups, 22, 8 and 7 varieties belong to group, I , II, and III, respectively (Table 2).

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Esterase Isozyme Variation in the Tissues of Glycine soja S. and Z. , Vigna vexillata var. tsusimensis Matsmura , and Trifolium repens L. in Korea (돌콩 , 돌동부 , 화이트 클로버의 조직별 Esterase Isozyme 변이에 관한 연구)

  • 이성규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.12 no.4
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    • pp.253-259
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    • 1992
  • The esterase isozyme in tissue of wild legume plants were separated by horizontal starch gel electrophoresis. Extracts used in this study were prepared from fully expanded young leaf, cotyledon and radicle of seedling and root-nodule of Glycine sola, Vigna vexillata var. tsuscmensis and Trifoliwn repens. The results are as follows; 1. Each tissue examined had a characteristic banding pattcrn. Number of bands in each species, G. soja, V. vexillata, and T . repens, were 14, 8 and 1 1 bands, respectively. And difference in esterase isozyme bands were greater from tissue to tissue than difference between habitat. 2. Est-I, Est-2. Est-3 and Est-4 in G. soja, Est-I in V. vexillata and Est-l and Est-2 in T. repens showed strong cnzyme activity than other enzyme. 3. Esterase isozyme variation in G. soja and T . repens showed more variety than V. vexillata. This is resulted from many genotypic differences within species. 4. The main enLyme among thc esterase isozyme were Est-I. Est-2, Est-3 and Est-4.

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Changes in Isozyme Patterns of Peroxidase and Esterase during the Microtuberization of Potato(Solanum tuberosum) (감자(Solanum tuberosum)의 기내 소괴경 형성 단계에 따른 Peroxidase와 Esterase 동위효소의 양상 변화)

  • 정현숙
    • Journal of Plant Biology
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    • v.36 no.1
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    • pp.51-57
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    • 1993
  • The microtuber was efficiently formed on SH medium containing 9% sucrose from the in vitro propagated shoot of potato (Solanum tuberosum cv. Sumi). In order to investigate gene expression depending on the development stage of microtuber, we examined the changes of peroxidase and esterase activities, and their isozyme patterns as well. Peroxidase and esterase activities were the highest at the 7 day-culture of the microtuber and subsequently decreased on the stage of microtuberization, whereas esterase activity increased at the stage of 60 day-culture. However, their activities in the ordinary tuber were higher than those of 60 day-cultured microtuber. In addition, in the peroxidase isozyme pattern two new bands of pI 7.05 and pI 4.65 were appeared at the 15- day and 60 day-cultures, respectively, as shown by isoelectric focusing. Various bands in the sterase isozyme pattern were shown at the 7 day-culture, and the band patterns were a large difference, comparing those of shoot and tuber. New bands in the esterase isozyme pattern also appeared at the 15 day- (pI4.52) and 60 day-cultures (pI 4.48). These results suggest that the changes of peroxidase and esterase activities and isozyme patterns are an important factor in the differentiation and development of potato.

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Esterase Isozyme Banding Pattern in Legume Seedings (콩과 식물의 Seeding Esterase Isozyme Banding Pattern에 관한 연구)

  • 이성규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.11 no.3
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    • pp.158-161
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    • 1991
  • Horizontal starch gel electrophoresis, follow by enzyrne-specific staining, separate and visualize several legume esterase isozyme. Using extracts prepared from cotyledon, radicle and plumule of legume seedlings germinated 5 days. The results were as follows. 1. The number and staining intensity of esterase isozyme bands varies depending on the plant species. tissues and developmental stage. 2. Bands in the cotyledon of field bean seedling expressed 4 and 1 in radicle. 3. In soybean cultivars, cotyledon of IIwangkum-kong had 3 bands and 1 band in the examined tissues of Paldal-kong and Jangkyung-kong seedling. 4. The cotyledon and radicle of french bean seedling had 3 bands, respectively. 5. The highest esterase isozyme activity appears to be expressed in the cotyledon and radicle of french bean, as indicated by intensity of stain, with the Paldal-kong particulary active.

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Comparison of Electrophoretic Isozyme Band Pattern of Pleurotus spp. in Korea -I. Homogeneous Gel- (한국산 느타리버섯(Pleurotus spp.)의 전기영동 Isozyme Band Pattern 비교 -I. Homogeneous Gel-)

  • Park, Yong-Hwan;Byun, Myung-Ok;Hiroshi, Fujii
    • The Korean Journal of Mycology
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    • v.16 no.2
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    • pp.87-94
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    • 1988
  • Electrophoretic isozyme patterns from mycelia, primordia, cap and stem of Pleurotus spp. collected in Korea were compared. Primordia, cap and stem of fruitbody showed very similar isozyme patterns but mycelial isozyme patterns were different from those of fruitbody. Isozyme patterns of malate dehydrogenase, acid phosphatase in Pleurotus ostreatus collected from different regions in Korea were similar but those of esterase, peroxidase, leucine amino peptidase and superoxide dismutase were different. Interspecific comparison of esterase isozyme patterns among Pleurotus ostreatus P. cornucopiae and, P. florida was very different and may be valuable subsidiary tool to conventional taxonomic techniques for identifying species of Pleurotus. A dendrogram by similarities of isozyme band pattern was presented.

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Enzyme-Htstochemical Studie5 of Esterase and Phosphatase on Developing Spirometra erinacei (Spirometra ernacei의 발육에 따른 Esterase와 Phosphatase의 조직 화학적 연구)

  • 곽기훈;김창환
    • The Korean Journal of Zoology
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    • v.31 no.3
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    • pp.225-235
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    • 1988
  • This study was carried out to compare distribution and isozyme pattern of nonspecific esterase, acid phosphatase and alkaline phosphatase on developing sparganum and adult of Spinometra erinacei by using enzyme-histochemical method and electrophoresis The sparganum and adult were recovered from rats and cat that were infected by sparganum. The results obtained were as follows: Nonspecific esterase had a strong activity in the parenchymal musculature of sparganum and adult, but no detectable level in ihe tegument. A total of 7 and 8 nonspecific esterase bands were detectable in sparganum and adult, respectively. Of these bands, band 3 and 4 were major bands in sparganum and adult. Acid phosphatase had a strong activity in the tegument and the epidermal musculature of sparganum, but no detectable level in the parenchymal musculature. A total of 3 bands were detectable in sparganum and adult. Of these bands, band 3 was major band in sparganum and adult. Alkaline phosphatase had a strong activity in the tegument and the epidennal musculature of sparganum and of adult, but no detectable level in the parenchymal musculature. A total of 2 and 4 bands were detectable in sparganum and adult. Of these bands, band 2 was major band in sparganum and adult. Based on the present results isozyme band patterns showed qualitative and quantitative changes in each tissues of sparganum and of adult during the development.

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Changes of Esterase Isozymes During the Development from Plodia interpunctella (Hiibner) (화랑곡나방의 발생에 따른 Esterase Isozymes의 Pattern변화)

  • Park, Hee-Yun;Lee, Hyung-Chul;Yoo, Chong-Myung
    • Journal of the Korean Society of Tobacco Science
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    • v.20 no.1
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    • pp.80-86
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    • 1998
  • Changes in activity and classification of esterase isozymes during the tire cycle or Plodia inteipunctella (Hiibner) were investigated by the native polyacrylamide gel electrophoresis. The stage specificity in esterase activity and isozyme pattern was observed throughout the larvalpupal-adult transformation. The activity esterase was highest at the 3-day old adult stage, and the lowest level at the prepupal stage. A total of 12 esterase bands were identified throughout the development, and the bands showing high enzyme activity was observed in the middle part of gel. Twelve esterases on the basis of inhibition by the three types of inhibitors (organophosphates, eserine sulfate and sulfhydryl reagents) were classified into three class, namely, carboxylesterase (CE), arylesterase (ArE) and cholinesterase (ChE), and these classes contained 7, 3 and 2 isozymes, respectively.

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Isozyme Patterns on Esterase, Peroxidase and Amylase in Benincasa hipspida (Thunb) Cogn and Commelina communis L. (동아와 달개비의 전기영동에 의한 Isozyme의 변이)

  • 채규인;최정식;김영두
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.2
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    • pp.174-176
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    • 1991
  • A electrophoretic study on seveal important enzymes was conducted with seed, leaf and stem of Benincasa hipida (Thunb) Cogn and Commelina communis L. Isozyme patterns of esterase were different from the parts and fruit of wax gourd appeared only one band. In the peroxidase isozyme, fruit of wax gourd appeared four bands, but stem of common dayflower no appeared. In the amylase isozyme, seed and fruit of 'gourd were appeared clearly one band, respectively.

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Esterase Isozyme Banding Pattern in Wild Legume Plants (야생 콩과식물의 Esterase Isozyme Banding Pattern에 관한 연구)

  • 이성규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.12 no.1
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    • pp.71-76
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    • 1992
  • Starch gel electrophoresis was used to examine the banding pattern of Esterase isozyme in the leaf, root-nodule and seedling of four wild legume species, Trifolim repense, Glycine soja, Phaseolus nipponensis and Vigna uexillata. The number of band, enzyme activity and migrating rate of esterase isozyme varies depending on the species and tissues of legume plants. The isozyme banding pattern in the cotyledon and radicle of T. repense showed same pattern, however, the number of band were varible among the cotyledon of G. soja, P. nipponensis and V. vexzllata, respectively. Est-1 in the leaf of G. soja, V, vexillata. root-nodule of G. soja and seedling of V. vexillata expreesed the highest enzyme activity. The Est-1 showed the rapidest migrating rate among the isozymes.

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