• Journal of Korean Society of Environmental Engineers
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• v.30 no.4
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• pp.385-392
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• 2008

In this study, seawater intrusion was assessed employing a kind of biological parameters such as Escherichia coli and Enterococcus faecalis while lab-prepared reclaimed water was recharged to prevent seawater intrusion. Chemical factors indicating seawater intrusion such as Cl$^-$, Ca$^{2+}$, Mg$^{2+}$ and specific conductivity were also simultaneously investigated where an ion exchange between a matrix in artificial aquifer and cations in solution was estimated. Both Escherichia coli and Enterococcus faecalis were shown to be very sensitive against degree of salinity during saline water intrusion. Enterococcus faecalis more strongly resisted against salinity than that of Escherichia coli. The ratio of Enterococcus faecalis divided by E. coli in the process of seawater intrusion increased up to more than 50$\sim$100 times in 18 hours whereas E. coli was died off more than 90% during pumping and recharge rate kept at 10 mL/min. However, when the rates of both recharge and pumping was kept at 5 mL/min, Enterococcus faecalis / Escherichia coli was sustained in the range of 2.5$\sim$5.0, while Escherichia coli showed dimished death rate. Chemical factors such as Cl$^-$, Ca$^{2+}$, Mg$^{2+}$ and specific conductivity showed more than 0.9 of high correlation each other well explaining the degree of seawater intrusion. The degree of ion exchange between artificial aquifer and saline water can be efficiently interpreted by both minus $\Delta$Na, $\Delta$Mg variation and positive $\Delta$Ca variation.

• Proceedings of the KIEE Conference
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• 2005.10a
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• pp.169-172
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• 2005

The simulation of Hydraulic pressure distribution of discharge tube with globular beads and the removal characteristic of Escherichia coli by the discharge tube with globular beads were estimated. The removal characteristic of Escherichia coli was related to the input voltage because the electric field is increased according to input voltage. As the passing amount of test water in discharge tube is increased, the removal ratio of Escherichia coli was increased because passing numbers of electric field section is increased.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.309.2-309.2
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• 2002

The FimH subunit of type l-fimbriated Escherichia coli has been determined as a major cause of urinary tract infection. To produce a possible vaccine antigen against urinary tract infection, the fimH gene was genetically coupled to the ctxa2b gene, which was then cloned into pMAL -p2E expression vector. The chimaeric construction of pMALfimH/ctxa2b was transformed into Escherichia coli TB1 and its N-terminal amino acid sequence was analyzed. (omitted)

• Journal of the Korean Chemical Society
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• v.37 no.7
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• pp.677-682
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• 1993

Polyacryloylcephalexine and polymethacryloylcephalexine were prepared by the reaction of polyacryloylchloride or polymethacryloyl chloride with cephalexine. The antimicrobial activities of these polymeric drugs were investigated by the common twofold dilution technique and the minimum inhibitory concentration (MIC) of polymeric durgs was also examined. Polyacryloylcephalexine revealed an excellent antibacterial activity against Micrococcus luteus ATCC 9341, Escherichia coli ESS, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Mycobacterium phlei IFO 3158, Klebsiella pueumouiae KCTC 1560, Escherichia coli KCTC 1039, Salmonella typhimurium KCTC 1925. Polymethacryloylcephalexine revealed an excellent antibacterial activity against Micrococcus luteus ATCC 9341, Klebsiella pueumouiae KCTC 1560, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Mycobacterium phlei IFO 3158, Escherichia coli KCTC 1039, Escherichia coli ESS, Salmonella typhimurium KCTC 1925.

• Korean Journal of Veterinary Research
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• v.30 no.4
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• pp.421-425
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• 1990

The present study was conducted to investigate the biochemical and cultural characteristics of Escherichia coli isolates from clinically affected chickens during the period from May 1988 to June 1989. A total of 82 E coli cultures were isolated from lesions of 75 chickens with colisepticemia. Biochemical properties of E coli isolates tested were in accordance with the general classification standard; all the isolates showed positive reaction in Catalase, Indol, and Methyl-Red tests, but negative reaction in Oxidase, Urease, $V{\ddot{o}ges$-Proskauer, Citrate utility, $H_2S$, Phenylalanine diaminase, and malonate tests. And the carbohydrate fermentation rates of them were shown to be variable. of the 82 isolates, 48(58.5%) cultures produced colicin to inhibit the indicator strain of E coli.

• Journal of Food Hygiene and Safety
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• v.22 no.2
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• pp.116-119
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• 2007

The antimicrobial susceptibility and antibiotic resistance patterns of 67 eae positive Escherichia coli strains isolated from pigs were investigated by disc diffusion method. Sixty-seven E. coli isolated from pigs showed susceptibility to Ceftiofur (98.5%), Lincomycin+Spectinomycin (74.6%), Danofloxacin (73.1%), Enrofloxacin (64.2%), and Neomycin (41.8%). However, the multiple resistance patterns were also seen in eae+E. coli isolates. Neomycin+Tylosin+Penicillin+Tetracycline, Tylosin+Penicillin+Tetracycline, and Neomycin+Tylosin+Danofloxacin+Penicillin+Tetracycline+Enrofloxacinwere the most prevalent patterns of multiple antibiotic resistance.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.54 no.4
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• pp.198-202
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• 2005

The simulation of hydraulic pressure distribution of discharge tube with globular beads and the removal characteristics of Escherichia coli by the discharge tube with globular beads were estimated. The removal characteristic of Escherichia coli was related to the input voltage because the electric field is increased according to input voltage. As the passing amount of test water in discharge tube is increased, the removal ratio of Escherichia coli was increased because passing numbers of electric field section is increased.

• Korean Journal of Microbiology
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• v.43 no.2
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• pp.116-123
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• 2007

The purpose of this study was typing the plasmid mediated extended spectrum ${\beta}-lactamases$ produced by enteric bacteria isolated from rivers in Pusan. Six strains of Eschericha coli and fifteen strains of Klebsiella pneumoniae transferred their plasmid mediated extended spectrum ${\beta}-lactamase$ genes to the recipient strain Eschericha coli J53 $Azid^{R}$. The plasmid mediated extended spectrum ${\beta}-lactamase$ genes were sequenced directly after PCR and the types were determined by the BCM Search Launcher and GenBank nucleotid database. Determined types of the plasmid mediated extended spectrum ${\beta}-lactamases$ were TEM-52 and SHV-12. TEM-52 was isolated from both Escherichia coli and Klebsiella pneumoniae. However SHV-12 was isolated from Klebsiella pneumoniae only. The results indicated that the plasmid mediated extended spectrum ${\beta}-lactamase$ producing bacteria spreded over the area of clinical to the nature in Korea.

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.421-424
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• 1992

Escherichia coli JM83 harboring penicilin G acylase gene of Bacillus megaterium ATCC14945 produced a protein in large amount (>20% of the total protein). The protein was identified as GroEL, one of the E. coli heat shock protein, by N-terminal amino acid sequence analysis. It was found that GroEL was induced by the expressed foreign penicilin G acylase at both 27 and $37^{\circ}C$.

• Journal of Microbiology and Biotechnology
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• v.6 no.2
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• pp.147-149
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• 1996

Poly(3-hydroxybutyrate) (PHB) was synthesized and accumulated intracellularly to a high concentration (7 g/l) by cultivating recombinant Escherichia coli XL1-Blue (pSYLl05) in a complex medium containing 20 g/l glucose. The morphology of PHB granules was examined by transmission electron microscopy. The PHB granules synthesized in recombinant E. coli were much larger than reported values for wild type microorganisms, and were often irregularly shaped. Some cells were apparently extruding PHB into the medium, which suggests that PHB granules maintain some fluidity and cells become fragile due to PHB accumulation.