• 대한의용생체공학회:의공학회지
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• 제37권1호
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• pp.46-52
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• 2016

나노 입자는 암의 조기 진단과 효과적인 치유를 위한 약물 전달체 또는 영상 조영제로 연구개발 되어왔다. 이러한 나노 입자는 주로 EPR effect로 인해 효과적으로 암 세포를 표적하는 것이 가능하다고 보고되었고, 주로 구형의 나노 입자들이 이러한 실험에 적용되었다. 그러나 최근 EPR effect가 사람의 암 치료에 있어서는 큰 효과를 나타내지 않는다는 한계점이 여러 연구를 통해 제시되고 있다. 따라서 본 연구에서는 이러한 구형 나노 입자의 한계점을 극복하기 위한 새로운 형태의 디스크 나노 입자를 제안하고, 디스크 나노 입자의 생산 과정과 입자의 질을 향상시키기 위한 입자 생산방법에 관한 연구 내용을 보고하였다. 본 연구를 통해 생산된 디스크 나노 입자는 인간 유방암인 MCF7 세포에 적용하여 항암제 전달체로써의 가능성을 평가하였다.

• 한국컴퓨터산업학회논문지
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• 제6권2호
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• pp.357-364
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• 2005

최근 들어 우리나라에서는 설치의 간편성과 높은 신뢰도를 가진 전력케이블의 사용이 증가하고 있다. 전력케이블은 출고 전에 IEEE std. 404-1993 시험을 거쳐 안정성을 확인하고 있지만 포설시 발생하는 접속부 내부의 결함으로 인하여 많은 문제가 발생하고 있다. 특히 불순물 혼입 또는 공극 발생시 고장율은 증가하게 된다 부분방전 검출은 포설 후 전력케이블의 상태를 관측할 수 있는 유용한 방법이다. 본 연구에서는 부분방전 특성을 평가하고자 케이블 접속재인 EPR과 케이블 절연체인 XPLE 사이에 인공 결함을 발생시킨 후 데이터 취득 시스템을 이용하여 $\Phi-q-n$ 특성을 검출하였으며, 부분방전의 정량적 해석을 위해 필요한 통계량을 계산하였으며, 신경망 모델을 적용하여 패턴 분석을 수행하여 $88\~96\%$의 구별이 가능하였다.

• 한국컴퓨터산업학회논문지
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• 제6권2호
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• pp.279-286
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• 2005

최근 들어 우리나라에서는 설치의 간편성과 높은 신뢰도를 가진 전력케이블의 사용이 증가하고 있다. 전력케이블은 출고 전에 IEEE std. 404-1993 시험을 거쳐 안정성을 확인하고 있지만 포설시 발생하는 접속부 내부의 결함으로 인하여 많은 문제가 발생하고 있다. 특히 불순물 혼입 또는 공극 발생시 고장율은 증가하게 된다. 부분방전 검출은 포설 후 전력케이블의 상태를 관측할 수 있는 유용한 방법이다. 본 연구에서는 부분방전 특성을 평가하고자 케이블 접속재인 EPR과 케이블 절연체인 XPLE 사이에 인공 결함을 발생시킨 후 데이터 취득 시스템을 이용하여 $\Phi-q-n$ 특성을 검출하였으며, 부분방전의 정량적 해석을 위해 필요한 통계량을 계산하였으며, 신경망 모델을 적용하여 패턴 분석을 수행하여 $88\~96\%$의 구별이 가능하였다.

• BMB Reports
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• 제30권6호
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• pp.397-402
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• 1997

A thiol-specific spin label was attached to cysteine-102 of yeast cytochrome c and electron paramagnetic resonance (EPR) spectra were measured as a function of added cytochrome c oxidase concentration. The intensity decreased due to line broadening as cytochrome c formed a complex with cytochrome c oxidase and reached a minimum when the ratio of cytochrome c to cytochrome c oxidase became one. Replacement of either Lys-72 or Lys-87 of cytochrome c by Glu did not result in a significant change in binding affinity. Interestingly the K72E mutant, unlike K87E, had a much lower rate of electron transfer than the wild type. These results indicate that many positively charged residues as a group participate in complex formation but Lys-72 might be important for cytochrome c to be locked in an orientation for an efficient electron transfer. A stoichiometry of 1 was also confirmed by optical absorption of the cytochrome c-cytochrome c oxidase complex which had been run through a gel chromatography cloumn to remove unbound cytochrome c. The EPR spectrum of this 1:1 complex, however, was a mixture of two components. This explains a biphasic kinetics for a single binding site on cytochrome c oxidase without invoking conformational transition.

• 한국전기전자재료학회논문지
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• 제28권8호
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• pp.501-507
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• 2015

In this paper, EPR (ethylene propylene rubber) insulation material was accelerated degradation test at $121^{\circ}C$, $136^{\circ}C$, $151^{\circ}C$, and experiment the typical EAB (elongation at break) at mechanical characteristics analysis. It is shown that the failure-time at the point of 50% of the initial value of Elongation rate to obtain the activation energy. The failure-time was shown each 5,219 hr, 3,165 hr, and 668 hr at three temperatures. In order to derive the activation energy, Arrhenius methodology was applied. Also, we got the Arrhenius plot from three accelerated temperatures. The activation energy values got 0.98 eV from EAB test. The experimental data were evaluated for estimating the probability density, and the suitable distribution by using statistical program MINITAB. It is shown that EAB data by the acceleration thermal degradation is most suitable for the Weibull distribution.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 춘계학술대회논문집A
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• pp.49-54
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• 2000

Cr-Mo-V steel is widely used as a material for the turbine structural component in fossil power plants. It is well known that this material shows the various material degradation phenomenons such as temper embrittlement, carbide coarsening. and softening etc. or ins to the severe operation conditions as high temperature and high pressure. These deteriorative factors cause tile change of mechanical properties as reduction of fracture toughness. Therefor it is necessary to evaluate tile extent of degradation damage for Cr-Mo-V steel in life assessment of turbine structural components. In this paper. the electrochemical potentiokinetic reactivation(EPR) test in $50wt%-Ca(NO_3)_2$ solution is performed to develop the newly technique for degradation damage evaluation of Cr-Mo-V steel. The results obtained from the EPR test are compared with those in small punch(SP) tests recommended by semi-nondestructive testing method using miniaturized specimen. The evaluation parameters used in EPR test are tile reactivation current density$(I_R)$ and charge$(Q_{RC})$ reactivation rate$(I_R/I_{Crit},\;Q_R/Q_{Crit})$. The results suggest that $I_R/I_{Crit}$ in these parameters shows a good correlation with SP test results.

• Ocean and Polar Research
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• 제40권3호
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• pp.115-125
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• 2018

Production from copepodite IV to adult of two euchaetid species Euchaeta plana and Paraeuchaeta russelli was measured at the southeastern sea of Korea from April to November, 2014. The mean density was $2.0ind\;m^{-3}$ for E. plana and $4.1ind\;m^{-3}$ for P. russelli, with the high contribution of copepodite V to total density. The densities of total individuals, adult females and eggs were highest in November for both species. The mean egg production rate (EPR) was $1.7eggs\;female^{-1}\;d^{-1}$ for E. plana and $3.1eggs\;female^{-1}\;d^{-1}$ for P. russelli. Both of them showed the highest EPR in September but zero EPR in summer. The mean weight-specific EPR was $0.038d^{-1}$ for E. plana and $0.079d^{-1}$ for P. russelli. The mean total production rates of E. plana and P. russelli were $5.3{\mu}g\;C\;m^{-3}\;d^{-1}$ and $17.8{\mu}g\;C\;m^{-3}\;d^{-1}$, respectively, with the largest production in November. The mean Production/Biomass ratio was $0.06d^{-1}$ for E. plana and $0.07d^{-1}$ for P. russelli, with its peak in September for both. The total production of E. plana and P. russelli was positively correlated with the density of a copepod Oncaea venusta, rather than chl-a concentration, indicating that the two copepods might be carnivores. This study evaluates the contribution of euchaetids to the copepod community in the southeastern sea of Korea.

• 분석과학
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• 제8권4호
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• pp.869-876
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• 1995

The active sites of the nickel and iron-containing enzyme, carbon monoxide dehydrogenase (CODH) from clostridium thermoaceticum were investigated using Electron Paramagnetic Resonance (EPR) technique. CODH exhibits several spectral features called NiFeC, $g_{ave}=1.82$, $g_{ave}=1.86$. FCII signals which are originated from different clusters in this enzyme. CODH is know to catalyze two different kinds of reactions - acetyl-CoA synthesis and CO oxidation. The acetyl-CoA synthesis activity can be followed by monitoring CO/acetyl-CoA exchange. The addition of 1,10-phenanthroline (phen) to CODH selectively destroyed the CO/acetyl-CoA exchange activity and eliminated the NiFeC signal completely. CO oxidation activity and other EPR signals were unaffected. Such behavior demonstrates that CODH has two distinct active sites and that the NiFe complex is only responsible for the CO/acctyl-CoA exchange activity. Phen caused the removal of only 30% of Ni in the NiFe complex ($0.3Ni/{\alpha}{\beta}$) as shown by the quantitative metal analysis. The phen-treated CODH could be reactivated fully by incubation In $Ni^{2+}$ solution. Radioactive $^{63}Ni^{2+}$ was used to quantitate the amount of the $Ni^{2+}$ incorporated into phen-treated enzyme and showed that the amount was the same as the removed by the phen treatment. i.e. $0.3Ni/{\alpha}{\beta}$. This indicates that only 30% of NiFe complexes are labile and responsible for the CO/acctyl-CoA exchange activity, the other 70% are non-labile and have no exchange activity. This is the first clear evidence that the NiFe complex is heterogencous and labile and non-labile Ni sites arc interacting differently with substrates and chelating agents like phen.

• Macromolecular Research
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• 제16권1호
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• pp.15-20
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• 2008

The enhanced permeability and retention (EPR) effect is used extensively for the passive targeting of many macromolecular drugs for tumors. Indeed, the EPR concept has been a gold standard in polymeric anticancer drug delivery systems. This study investigated the tumoral distribution of self-assembled nanoparticles based on the EPR effect using fluorescein and radio-labeled nanoparticles. Self-assembled nanoparticles were prepared from amphiphilic chitosan derivatives, and their tissue distribution was examined in tumor-bearing mice. The size of the nanoparticles was controlled to be 330 run, which is a size suited for opening between the defective endothelial cells in tumors. The long-circulating polymer nanoparticles were allowed to gradually accumulate in the tumors for 11 days. The amount of nanoparticles accumulated in the tumors was remarkably augmented from 3.4%ID/g tissue at 1 day to 25.9%ID/g tissue at 11 days after i.v. administration. The self-assembled nanoparticles were sustained at a high level throughout the 14 day experimental period, indicating their long systemic retention in the blood circulation. The ${\gamma}$-images provided clear evidence of selective tumor localization of the $^{131}I$-labeled nanoparticles. Confocal microscopy revealed the fluorescein-labeled nanoparticles to be preferentially localized in the perivascular regions, suggesting their extravasation to the tumors through the hyperpermeable angiogenic tumor vasculature. This highly selective tumoral accumulation of nanoparticles was attributed to the leakiness of the blood vessels in the tumors and their long residence time in the blood circulation.

• Nuclear Engineering and Technology
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• 제55권12호
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• pp.4576-4582
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• 2023

The Korea retrospective dosimetry (KREDOS)-electron paramagnetic resonance (EPR) group undertook an intercomparison investigation utilizing hydroxyapatite. This analysis involved four institutions: the Korea Institute of Radiological and Medical Sciences, Dongnam Institute of Radiological and Medical Sciences, Korean Association for Radiation Application, and Radiation Health Institute of Korea Hydro & Nuclear Power. Following the irradiation of the hydroxyapatite sample, the recorded build-up was analyzed. To validate the reliability of the EPR dosimetry findings and enhance its operational performance, a hydroxyapatite dose-response curve was plotted and dosimetry was performed for a blind sample. The proficiency of each laboratory was assessed by employing an interlaboratory comparison methodology. This involved a comparative analysis of the measurement results by calculating the relative bias, z-score, and En value. The results submitted by the participating laboratories demonstrated satisfactory ratings for doses of 1.006, 3.999, and 6.993 Gy. Following the second intercomparison, efforts to optimize their hydroxyapatite-EPR dosimetry systems are underway in the participating laboratories. The current assessment of hydroxyapatite dose yielded the foundational data required to establish the parameters of dental dosimetry. In future, the third intercomparison experiment will be conducted for exploring other materials.