• 제목/요약/키워드: environmental induction

검색결과 773건 처리시간 0.029초

Adverse Reproductive Effects on Plasma Vitellogenin and Sex Steroid Levels, and Gonadosomatic Index in Juvenile Common Carps (Cyprinus carpio) Exposed to 17$\beta$-Estradiol and D-2-Ethylhexyl Phthalate

  • Seo, Jinwon;Park, Kyung-Seo;Moon, Woon-Gi;Lee, Sung-Kyu
    • 한국환경생물학회:학술대회논문집
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    • 한국환경생물학회 2002년도 학술대회
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    • pp.141-144
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    • 2002
  • Environmental estrogens are natural or synthetic substances present in the aquatic environment, especially in effluent from sewage treatment. However, the adverse effects of these estrogenic substances on fish reproduction are unknown. Di-2-ethylhexyl phthalate (DEHP) is the most common phthalate, which Ps used as a plasticizer in polyvinylchloride (PVC), and it is widespread in the environment and has been found in aquatic organisms and sediments. Therefore, juvenile common carps (Cyprinus carpio) were exposed to nominal concentrations of 17$\beta$-estradiol (E2) (0.5, 5, 50 $\mu\textrm{g}$/L) and DEHP (10, 100, 500 $\mu\textrm{g}$/L) for 21 days, to determine the adverse reproductive effects of these compounds on plasma vitellogenin (VTG) induction, sex steroid level, and gonad weight. Electrophoresis (SDS-PAGE) revealed that much of VTG was induced in fish exposed to 5 and 50 E$_2$ $\mu\textrm{g}$/L, but none of DEHP exposure showed induction. Enzyme-linked immunosorbent assay (ELISA) revealed that VTG was significantly induced in fish exposed to 5 and 50 E$_2$ $\mu\textrm{g}$/L, and combination of 50 E$_2$ $\mu\textrm{g}$/L with 10 and 500 DEHP $\mu\textrm{g}$/L, but none of DEHP exposure showed induction. Analysis of sex steroid levels in some fish revealed that testosterone (T) was detected in both male and female fish of the control and DEHP exposures, but none of fish exposed to 22 concentrations had detectable testosterone level. On the other hand, E$_2$ exposure induced 17$\beta$-estradiol in plasma of male fish, but there was no induction of 17$\beta$-estradiol in plasma of male fish exposed to DEHP. Comparison of gonadosomatic index (GSI) revealed that maximal E$_2$ exposure inhibited ovarian growth, but maximal DEHP exposure stimulated testicular growth. The results indicated that those comparisons can be a useful bio-indicator for determining adverse reproductive effect of endocrine disrupting chemicals (EDCs).

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국소 허혈성 뇌손상 흰쥐 모델에서 환경강화 조건 하 수정된 건측억제유도 운동치료가 앞다리 운동기능 증진에 미치는 영향 (Effects of Improved Forelimb Sensorimotor Function on the Modified CIMT Applied Under the influence of Environmental Enrichment in a Focal Ischemic Brain Injury Rat Model)

  • 이삼규;김계엽;남기원;오명화;김용억;김은정;장미경;김경윤;정현우;김종만
    • 한국전문물리치료학회지
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    • 제14권3호
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    • pp.48-56
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    • 2007
  • Environmental Enrichment (EE) alone is not capable of enhancing the fine digit and the forelimb functions. Therefore, we applied modified constraint-induced movement therapy (mCIMT) under the influence of EE to assess its effect on promoting improved forelimb sensorimotor functions. Focal ischemic brain injury was produced in Sprague-Dawley rats (60 rats, $250{\pm}50$ g) through middle cerebral artery occlusion (MCAO). Before MCAO induction, all rats were trained in modified limb placing tests and reaching tasks for 1 week. Then they were randomly divided into three groups: Group I: application of standard environment (SE) after MCAO induction (n=20), Group II: application of EE after MCAO induction (n=20), Group III: MCAO+EE, mCIMT and task-oriented training that was initiated at 10th day after MCAO induction (n=20). We also applied mCIMT (between 9 AM and 5 PM/daily) which included restraining the forelimb ipsilateral to the lesion using the 'Jones & Schallert' method. We assessed the change of modified limb placing, single pellet reaching test and the immunoreactivity of BDNF by immunohistochemistry (pre, 1st, 5th, 10th and 20th day). Group I showed no improved outcome, whereas group II and III significantly improved on the use of the forelimb and the immunoreactivity. The qualitative analysis of the skilled reaching test, of group III showed the greatest improvement in the fine digit and the forelimb function. These results suggest that EE combined with mCIMT is more functional in promoting enhanced fine digit and forelimb functional movements.

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단체급식소에서의 환경운동이 환경문제 인식도와 환경보호 실천도에 미치는 영향 (The Effect of Environmental Campaign on the Recognition of Environmental Problem and the Execution of Environmental Protection in Foodservice)

  • 전무영;민혜선
    • 대한영양사협회학술지
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    • 제6권2호
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    • pp.71-78
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    • 2000
  • Environmental pollution induced by food wastes is considered as one of very serious problems in the world, and it is the most important to reduce the production of food wastes. In this study, environmental campaign for reducing food waste was conducted by applying various campaign methods using such as a bulletin board, intra-network, slogans & posters and news letters, as well as some systems such as penalty and prize in a business & industry foodservice. We investigated customers' recognition and execution degree before and after environmental campaign for the purpose of analyzing the changes of customers' attitude by the campaign. The subjects of this study had generally high level of recognition of environmental problem(3.09 point) compared to the execution degree(1.88 point)(Max. 5 points), implying necessity for the induction of actual execution of food wastes reduction by continuous environmental campaign. After environmental campaign, the recognition of environmental problem related to food wastes was significantly increased from 3.09 point to 3.29 point (p<0.001), and the execution degree for food wastes reduction was also greatly increased from 1.88 to 2.70 point (p<0.001). These changes indicated that campaign for food wastes reduction has raised customers' recognition and execution for environmental protection.

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MRAS 기법을 이용한 권선형 유도전동기의 속도센서리스 벡터제어 (Speed Sensorless Vector Control of Wound Induction Motor Using a MRAS Method)

  • 최현식;이재학;엄태욱
    • 대한전자공학회논문지TE
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    • 제42권1호
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    • pp.29-34
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    • 2005
  • 권선형 유도전동기는 기동시에 충분히 큰 저항을 외부에서 삽입하여 기동전류를 작게하는 동시에 기동토크를 크게 할 수 있다. 또한, 유도전동기의 각 기동방식중에서 가장 우수한 시동특성을 가지고 있으며 크레인, 시멘트공장 등 중부하 시동이 요구되는 경우 널리 사용되고 있다. 권선형 유도전동기 드라이브 시스템의 전류, 토크, 위치 및 속도 등의 제어를 위하여 일반적으로 산업현장에서는 PI 제어기가 많이 적용되고 있다. 그러나 이러한 시스템은 센서 부착시 여러가지 환경적 제약으로 인한 전체시스템의 성능 저하를 가져올 수 있어 이를 개선하기 위한 센서리스 벡터제어가 활발히 연구되고 있다. 본 논문은 권선형 유도전동기의 센서리스 벡터제어를 위해 MRAS 기법을 적용하였고, 기존 MRAS에 의한 유도전동기 속도 센서리스 제어시 발생하는 저항 값의 변화에 따른 저속 영역에서의 속도 특성 악영향을 개선키 위해 권선형 유도전동기의 고정자 저항과 회전자 저항 값을 추정하는 제어 알고리즘을 추가하여 시스템의 동특성 개선을 시도하였다. 제안된 기법의 타당성 및 유효성을 컴퓨터 시뮬레이션으로 확인하였다.

Diel Pattern of Littoral Swarming in Moina macrocopa and Impact of Juvenile Fish Density

  • Jeong, Hyun-Gi;La, Geung-Hwan;Kim, Hyun-Woo;Jang, Min-Ho;Jeong, Kwang-Seuk;Yoon, Ju-Duk;Joo, Gea-Jae
    • 생태와환경
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    • 제43권4호
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    • pp.513-517
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    • 2010
  • The diel swarming of Moina macrocopa and the relationship between environmental factors were evaluated in a shallow reservoir. The littoral density of Moina macrocopa maintained low from night to noon, reached maximum density at the afternoon with compact swarms at the waters' edge, and dispersed after sunset. The recruitment of Moina macrocopa and changes of water temperature (r=0.709, p<0.001) and juvenile fish density (r=0.511, p=0.002) in the littoral zone showed a significant positive relationships. After the induction of diel horizontal migration toward littoral zone, therefore, direct juvenile fish predation pressure should be induced Moina macrocopa swarming.

억새(Miscanthus sinensis) 성숙 종자로부터의 캘러스 유도 및 식물체 재분화 체계 확립 (Establishment of Callus Induction and Plant Regeneration System from Mature Seeds of Miscanthus sinensis)

  • 조준형;변지희
    • 한국자원식물학회지
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    • 제24권5호
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    • pp.628-635
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    • 2011
  • 본 연구는 최근 한방자원, 사료자원, 바이오에너지 자원 등 다양하게 이용되는 국내 자생 억새(Miscanthus sinensis)의 대량생산 및 신품종 개발을 위한 조직배양체계 확립을 위해 수행되었다. 이를 위해 억새 완숙종자로부터의 캘러스 유도와 재분화를 위한 식물생장조절제의 적정농도를 규명하였다. 억새의 성숙종자유래 배발생 캘러스 유도를 위해 2,4-D, IBA, NAA를 1~10 $mg{\cdot}L^{-1}$의 농도로 단용 처리한 결과, 5 $mg{\cdot}L^{-1}$ 2,4-D 처리에서 가장 높은 85.3%의 캘러스 유도율과 캘러스의 증식을 보였으며 조직배양 과정 중 갈변화율도 가장 낮았다. 또한, 캘러스의 재분화를 위해 옥신인 NAA와 Kinetin, 2-iP, 또는 BAP 등의 사이토키닌을 혼용 처리한 결과, 각각 19.0%~59.0%, 23.0%~67.3%, 14.7%~83.7%의 재분화율을 보여 NAA와 BAP의 혼용 처리구가 NAA와 Kinetin 또는 2-iP와 혼용 처리구보다 식물체 재분화에 효과적이었다. 특히 3 $mg{\cdot}L^{-1}$ NAA와 5 $mg{\cdot}L^{-1}$ BAP 혼용 처리된 배지에서의 재분화율이 83.7%로 가장 높게 나타났으며, 캘러스 당 재분화 식물체 개수도 5.5개로 동일농도의 2-iP 또는 Kinetin 혼용 처리 시 2.1 및 2.0개보다 많았다. 본 연구결과 억새 성숙 종자로부터의 배발생 캘러스 유도에는 5 $mg{\cdot}L^{-1}$ 2,4-D가 그리고 캘러스의 재분화에는 3 $mg{\cdot}L^{-1}$ NAA와 5 $mg{\cdot}L^{-1}$ BAP 혼용 처리가 가장 효율적이었다. 본 연구를 통해 확립된 조직배양체계는 억새의 대량생산 및 신품종 개발에 유용할 것으로 판단된다.

Aluminum oxide, calcium oxide, sodium tetraborate 처리에 의한 설치류 골수세포의 소핵유발 연구 (A Study of Mammalian Erythrocyte Micronucleus Induction with Aluminum Oxide, Calcium Oxide, Sodium Tetraborate)

  • 임경택;김수진;김종규;강민구;김현영;양정선
    • Environmental Analysis Health and Toxicology
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    • 제24권2호
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    • pp.127-136
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    • 2009
  • We have investigated the genotoxicity of 3 chemicals, aluminum oxide, calcium oxide, sodium tetraborate using mammalian erythrocyte with micronucleus induction. It was performed using 9 week male ICR mice. At 24 hours after treatment with 3 chemicals with oral route, mice were sacrificed and bone marrow cells were prepared for smear slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes (PCE), all treatment groups did not show statistically significant increase than negative control group. And there was no clinical sign related with injection of the 3 chemicals. It was concluded that the 3 chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and these results indicate that the 3 chemicals have no mutagenic potential under the condition in each studies.