• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.31 no.5
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• pp.385-391
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• 2013

As a solution for environmental problems caused by increasing number of vehicles, it is encouraged to use a bicycle as an environment-friendly transportation method. To vitalize the bicycle usage, it is a necessary to construct bicycle roads that are safe and suitable for users. Based on the previous research results, we assume the main purposes of bicycle usages are mainly local leisure activity and school commuting. Thus, the proposed method finds the shortest link between the existing bicycle road network and bicycle usage facilities such as leisure activity places or schools over public road network. Then, we carry out the RTK DGPS survey for the candidate links, and analyze the slopes of them. When the slope of a found link is larger than a threshold, an alternative link is re-found for the safety and convenience of a bicycle user. The proposed method is applied to the real bicycle road network in Mokpo, Chunnam and the results are discussed.

• Korean Journal of Organic Agriculture
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• v.12 no.2
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• pp.231-241
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• 2004

In recent years, many researches are actively undertaken for environmental-friendly animal production according to the increased understanding about food safety because of the outbreak of various diseases such as mad cow disease, Foot and mouth disease and Poultry Influenza virus. However, high quality(higher safety)- animal production may not be successful without increasing of disease resistance of animal and the improvement of feeding environment. To increase the disease resistance is able to be accomplished by stimulating the immune function. The present study was undertaken to investigate the effects of enzyme mixture reinforced with ${\beta}$-glucanase activity which degrade polysaccharide to release ${\beta}$-glucan known as stimulator of immune function on the change of milk production and somatic cell count. After 12weeks of experimental feeding, milk production tended to be increased and somatic cell count was decreased from average $227{\times}10^4$ to $37.1{\times}10^4$. Milk protein and solid-fat content were tended to increase but milk fat showed decreasing tendency by the feeding of enzyme mixture. All together, it has been suggest6d that the improvement of high quality milk production may be possible through the dietary addition of immune modulating enzyme mixture in lactating dairy cows.

• Journal of the Korean Electrochemical Society
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• v.23 no.3
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• pp.73-80
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• 2020

Fenton oxidation method using hydrogen peroxide is an eco-friendly oxidation method used in water treatment and soil restoration. When removing pollutants by this method, it is quite important to properly regulate the concentration of hydrogen peroxide according to the concentration of the contaminants. In this study, electrochemical biosensors using HRP (horseradish peroxidase) enzymes were manufactured and studies were conducted on the activity of enzymes and the detection characteristics of hydrogen peroxide. HRP were electro deposited with chitosan and AuNP on the working electrode surface of the SPCE (Screen Printed Carbon Electrode). Then, the fixation of enzymes was confirmed using the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The activity of HRP enzymes was also identified from chronoamperometry (CA) and UV spectroscopy. After immersing the biosensor in PBS solution the current generated from electrodes by titrating hydrogen peroxide was measured from CA analysis. The generated current increased linearly for the concentration of hydrogen peroxide, and a calibration curve was derived that could predict the concentration of hydrogen peroxide from the current.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.313-322
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• 2022

Seed-borne pathogens in crops reduce the seed germination rate and hamper seedling growth, leading to significant yield loss. Due to the growing concerns about environmental damage and the development of resistance to agrochemicals among pathogen populations, there is a strong demand for eco-friendly alternatives to synthetic chemicals in agriculture. It has been well established during the last few decades that plant seeds harbor diverse microbes, some of which are vertically transmitted and important for plant health and productivity. In this study, we isolated culturable endophytic bacteria and fungi from soybean seeds and evaluated their antagonistic activities against common bacterial and fungal seed-borne pathogens of soybean. A total of 87 bacterial isolates and 66 fungal isolates were obtained. Sequencing of 16S rDNA and internal transcribed spacer amplicon showed that these isolates correspond to 30 and 15 different species of bacteria and fungi, respectively. Our antibacterial and antifungal activity assay showed that four fungal species and nine bacterial species have the potential to suppress the growth of at least one seed-borne pathogen tested in the study. Among them, Pseudomonas koreensis appears to have strong antagonistic activities across all the pathogens. Our collection of soybean seed endophytes would be a valuable resource not only for studying biology and ecology of seed endophytes but also for practical deployment of seed endophytes toward crop protection.

• Journal of Applied Biological Chemistry
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• v.52 no.3
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• pp.116-120
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• 2009

We found out the new method of the consortium for the environmental friendly agriculture by 8 kinds of the selected antagonistic rhizobacteria. This research involved composition of mutual complementary consortium by each antagonistic function such as production of antibiotic, siderophore, antifungal cellulase and insoluble phosphate solubilization. The consortium No.11 among composed consortium candidates showed the most pepper growth promoting activity and Phytophthora blight suppression on the in vivo pot test of red-pepper plant. The consortium No. 11 is combination of PGPR Bacillus subtilis AH18 and Bacillus licheniformis K11. B. subtilis AH18 and B. licheniformis K11 both could produce the auxin, antifungal ${\beta}$-glucannase and siderophore. Also, they had mechanism for solubilization of insoluble phosphate. But, B. licheniformis K11 could produce the antibiotic of iturin which was able to inhibit Phytophthora capsici. We confirmed complementary noncompetitive mutualism between B. subtilis AH18 and B. licheniformis K11 of the consortium No.11. The results came out through treatment of two strains co-culture, treatment of individual culture and co-treatment of two individual cultures for the growth and Phytophthora blight suppression of red-pepper. The treatment of two strains co-culture didn't show a synergic effect in comparing sole treatment on the pepper growth promotion and Phytophthora blight suppression. But, when the pots were treated simultaneously with co-treatment of two individual cultures, an synergic effect was seen in the growth promotion of roots, stem, leaves and suppressed Phytophthora blight on red-pepper in vivo pot test.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.303-309
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• 2011

This study was carried out to investigate the evaluation of insecticidal efficacy and control effect in field of 10 environmental-friendly agricultural materials (EFAM) and seasonal occurrence at the vineyards to the Lycorma delicatula. L. delicatula nymphs occurred from late May to late August the highest early June (1st nymph) while adults occurred from late July to November with the highest peak of early October (preovipositing female) in 2010. Total population density of L. delicatula was the highest in early June. For the 3rd~4th nymphal instars and adults, EFAM (Spider, Jindikap-plus and Byejin-${\alpha}$) showed perfect insecticidal activity 2hour after treatment. Seoncho showed insecticidal activities 96.7% within 48 hour. Residual effects between EFAM showed 55.5% Spider at recommended concentration at 7 days after treatment(DAT), the other EFAM had low efficacy. Jindikap-plus and Spider showed 99% control value on nymphs in the field test at 3DAT, appeared control value more than 90% at 7DAT. Also, the control effects of Jindikap-plus and Spider were showed the adult mortality of 100% and 98.2% respectively at 3DAT, 90.6% and 84.6% respectively at 7DAT. Whereas the other EFAM had low efficacy. Jindikap-plus and Spider were excellent control effects compared with Acetamiprid WP.

• Korean Journal of Environmental Biology
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• v.41 no.4
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• pp.463-472
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• 2023

The insecticidal activities of 27 different commercial products with environmentally friendly organic material(EFOM) against Scotinophara lurida, a major rice pest, were evaluated in the laboratory using spraying methods on plants and insects. Seven plant-derived organic farming materials (EFOM-8, -10, -12, -13, -19, -20, and -26) with high insecticidal effects when sprayed directly on the insect's body rather than on the plant were selected. In the indoor rice pot test, all 7 EFOMs showed an insecticidal rate of over 73.3% under flooding conditions. Notably, EFOM-13 and EFOM-20 demonstrated much higher insecticidal rates, ranging from 1.5 to 1.8 times, in flooding conditions compared to drained conditions. In the semi-paddy field test, EFOM-10 (80% garlic extract), EFOM-13 (62% neem extract), and EFOM-26 (70% sophora extract+28% ethyl alcohol+2% pyrethrum extract) exhibited a higher control value of 88.9% in the irrigated paddy on the 7th day, surpassing the control values in the drained paddy by 1.4 to 1.9 times. The control value in the irrigated rice paddy field sprayed with EFOM-10 reached 86.2% on the 7th day, which was 1.4 times higher than 61.9% in the drained paddy. Taken together, the findings suggest that direct contact of the insect's body with sufficient amounts of spray solution and the maintenance of paddy irrigation can enhance the controlling effect of EFOMs. These findings will be valuable in developing an optimal S. lurida control strategy for application in rice paddy fields in the near future.

• Journal of Environmental Science International
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• v.20 no.5
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• pp.631-638
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• 2011

The sense of crisis on seriousness of earth environment is gradually getting greater and the importance of environment education followed by this trend is being highlighted. As an environment friendly New Environmental Paradigm opposed to the human Dominant Social Paradigm on the existing environment is on the rise, the environment education considers not only the perceptive field, but the definitive field and behavioral pattern to follow as important values. Accordingly, the basic awareness of students on environment was found out in order to perform a proper environment education and tried to find out the behavior pattern that the students have on environment on this basis. For the research tool of this study, the one that has revised the Revised NEP Scale for elementary school students which Geum, Ji Hun Kim, Jin Mo have validated the feasibility and reliability while the test paper was prepared by dividing into recycling behavior, energy preservation behavior and source of information. The target of this study was 600 students in sixth grade of elementary school residing in Kimhae, Gyeongsangnamdo while the mean, average, standard deviation, independent t-test and correlation analysis were performed using SPSS 15.0 statistics program for a total of 462 copies including 235 copies from male students and 227 copies from female students. The results of this study are as follows. First, everyone had high pro-NEP tendencies on the environmental problem with 4.14. On the other hand, the anti-NEP did not have high awareness standard with 2.98. Second, the awareness level on resource recycling was shown as being quite high with 4.05. Between NEP and resource recycling, a positive correlation of r=.155 was shown so that the awareness level on resource recycling was getting quite high as the awareness on environment problem was getting higher. Third, the awareness level on energy saving activity was shown as being very high with 3.0. Between NEP and resource recycling, a positive correlation of r=.210 so that the awareness level on energy saving was getting quite high as the awareness on environment problem was getting higher. Fourth, for the source of information on environmental problems of region, our country and global village, the television and internet were shown as main sources of information while the ratio of positive answers on teachers and region as sources of information was shown as quite low.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.381-387
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• 2014

BACKGROUND: Garlic (Allium sativum) extract has been allowed as commercial biopesticide material for pesticidal activity in the Environmentally-friendly Agriculture Promotion Act. Nine commercial biopesticides containing A. sativum extract have been marketed in Korea. However, the analytical method of the active substances in these materials has not been studied. METHODS AND RESULTS: Cartridge clean-up method for the determination of dimethyl disulfide(DMDS), diallyl disulfide(DADS), and diallyl trisulfide(DATS) in biopesticides containing A. sativum extract was developed and validated by gas chromatography(GC). The clean-up method was optimized using hydrophilic lipophilic balance (HLB) solid phase extraction(SPE) cartridges for the bioactive sulfides in biopesticides containing A. sativum extract, and the eluate was analyzed to quantify the DMDS, DADS, and DATS using the GC. The developed method was validated, and the LOQ and recovery rates of DMDS, DADS, and DATS were 0.226, 0.063, and $0.051mg\;L^{-1}$ and 80.6, 84.8, and 73.1%, respectively. From the nine commercial biopesticide samples, contents of DMDS, DADS, and DATS were analyzed using the developed method and results showed $2.3mg\;L^{-1}$, respectively. CONCLUSION: The developed method could be used in determining the quality of biopesticides for the manufacture of commercial biopesticides containing A. sativum extract.

• Journal of the Mineralogical Society of Korea
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• v.29 no.2
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• pp.35-45
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• 2016

Clays, which are abundant in nature and eco-friendly, have been utilized throughout human history due to their characteristic physicochemical properties. Recently, a variety of clays such as montmorillonite, kaolinite, sepiolite and layered double hydroxide with or without chemical modification have been extensively studied for potential application in industries. Clays that possess a large specific surface area, high aspect ratio, nanometer sized layer thickness and controllable surface charge could be utilized as polymer fillers after appropriate chemical modifications. These modified clays can improve mechanical and gas barrier properties of polymer materials but also provide sustained antibacterial activity to polymer films. Furthermore, engineered clays can be utilized as scavengers for chemical or biological pollutants in water or soil, because they have desirable adsorption properties and chemical specificity. In this review, we are going to introduce recent researches on engineered clays for potential applications in future industries such as food packaging and environmental remediation.