• 한국식품위생안전성학회지
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• 제28권4호
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• pp.287-292
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• 2013

오염된 식품으로부터 바이러스를 효과적으로 검출하기 위해서는 식품에 부착된 바이러스를 효과적으로 elute시키는 것이 결정적으로 중요하다. 본 연구에서는 채소류에 공통적으로 적용할 수 있는 elution용액을 찾기 위해 폴리오 바이러스를 인위적으로 오염시킨 1가지 엽채류 (깻잎) 와 3가지 근채류 (당근, 양파, 무) 로부터 바이러스 회수율을 조사한 후, 최적의 바이러스 회수조건을 찾기 위해 기보고된 상추와 양배추의 회수율을 함께 분석하였다. 바이러스의 회수율은 식품의 matrix와 사용된 elution용액의 종류에 따라 차이가 컸으나 0.25M threonine / 0.3M NaCl (pH 9.5) 또는 0.25M glycine / 0.14M NaCl (pH 9.5)을 사용하였을 때 6가지 채소 중 5가지로부터 폴리오바이러스를 효과적으로 elute 할 수 있었다. 0.25M threonine / 0.3M NaCl(pH 9.5)를 노로바이러스 검출에 적용해 본 결과 근채류인 당근보다 엽채류인 깻잎으로부터 노로바이러스 GII를 더 잘 검출할 수 있었다. 이 같은 공통 elution용액을 사용할 경우 다양한 종류의 채소류에 오염된 노로바이러스를 포함한 소화기바이러스의 검출을 용이하게 해 줄 것이다.

• Journal of Microbiology and Biotechnology
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• 제28권3호
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• pp.465-472
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• 2018

Our aim was to determine the detection rate of respiratory viruses (RVs) in feces of patients with acute viral respiratory infection (AVRI) and the detection rate of diarrheal viruses (DVs) in nasopharyngeal samples from patients with acute viral gastroenteritis. The relationships between the presence of fecal RVs or nasopharyngeal DVs and their impacts on the clinical severity were also investigated. A total of 144 fecal specimens were collected from AVRI patients and 95 nasopharyngeal specimens were collected from acute viral gastroenteritis patients. Clinical characteristics and laboratory profiles were compared between subgroups on the basis of the presence or absence of virus in the specimens. The detection rate of RVs in feces was 17.4% (25/144), whereas the detection rate for viruses identical to the respiratory pathogen was 10.4% (identical group, 15/144). Within the identical group, adenovirus (86.7%, 13/15) was most commonly found. Patients in the identical group showed statistically higher values for C-reactive protein, mean age, increased frequency of vomiting, and decreased frequency of chest film involvement and cough (p < 0.05). The detection rate of nasopharyngeal DVs among acute viral gastroenteritis patients was 19.0% (18/95), and in the identical group it was 15.8% (15/95). Norovirus group II and enteric adenovirus were the major pathogens detected in the identical group. There were no significant differences in clinical characteristics and laboratory profiles between the subgroups. In conclusion, the major pathogens of fecal RV and nasopharyngeal DV were adenovirus and norovirus group II, respectively. However, their relationship with the clinical symptoms or disease severity is unclear.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.57.1-57.1
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• 2006

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.57.2-57.2
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• 2006

• 한국동물위생학회지
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• 제22권1호
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• pp.43-52
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• 1999

Presently, viral isolation in the diarrheal feces can be reached by many tools such as fluorescent antibody test(FA), negative contrast electron microscopy(NCEM), virus neutralization test, cell culture, and so on. The purpose of the study was to aimed at the establishment of simplified NCEM technique which can be efficiently applied for diarrheal feces and also the understanding on prevalence of viral-induced diarrhea in calves. One hundred fourty-seven korean indigenous calves with diarrhea were examined to their feces by the modified NCEM. Among them, 98(66.7%) were confirmed to have one or more viruses in feces. The viruses detected were identified as rotavirus(33.3%), coronavirus(16.3% ), togavirus(10.2%) and herpesvirus(0.7%). Ten cases of combined viral infection were consisted of 8 with rotavirus+coronavirus, one with rotavirus+togavlrus and one with rotavirus+herpesvirus. Dirrheal types could classified by yello-wish watery(44.9a ), blood-tinged(19.7% ), white watery(17.7% ) , brownish watery(14.3%), greenish watery(3.4%) diarrhea, respectively. Yellowish watery diarrhea(66cases) was frequently included rotavirus(31.8%), coronavirus(15.2%), and togavirus(13.6%), respectively. Consequently, these results suggest that the modified NCEM is reliable and efficient diagnostic tool for detection of viruses in the diarrheal feces and many calves rearing in Chonnam province have been exposed to some enteric viral agents mainly including rotavirus and coronavirus.

• 한국미생물·생명공학회지
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• 제47권2호
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• pp.317-322
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• 2019

Gastroenteritis with diarrhea is one of the most infectious diseases in the world following respiratory infections. Notably, diarrhea-causing viruses (DVs) cause more than 70% of such cases. In this study, 3,065 stool specimens from patients with diarrhea (median age, 1.1 years; range, 0.0-91.1 years), who were admitted to the DanKook University Hospital, were examined using multiplex reverse transcription PCR (mRT-PCR). The target viruses were astrovirus (AstV), enteric adenovirus (EAdV), group A rotavirus (RotV), norovirus GI (NoV-GI), and norovirus GII (NoV-GII). The mRT-PCR results were analyzed based on various factors such as seasonality, age, presence of co-infection, and analyzed trends. The detection rate of the DVs during the study period was found to be 30.8% (n = 943/3,065). When the detection rate was analyzed monthly, the DV detection rate was found to be highest between December to January. Of the detected DVs, NoV-GII was the most common, accounting for 45.5% of the detected viruses (n = 446/980). Notably, 86.5% (n = 848/980) of the pathogens were detected in individuals who were less than 5 years of age. During the study period, NoV-GII and RotV showed alternating trends. In addition, both the number and rate of co-infections increased.

• 생명과학회지
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• 제19권3호
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• pp.373-377
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• 2009

환경시료에서 많이 분리되는 장내바이러스 중 폴리오바이러스, 콕사키바이러스, 에코바이러스를 이용하여 정수처리 공정별 제거율을 조사하였다. 양성 시료로 사용된 바이러스들의 회수율은 72-108%의 범위로 나타났다. 이들 3종 바이러스들을 대상으로 정수처리공정별 제거효율을 조사한 결과 약 99%의 바이러스가 침전단계에서 제거되어졌고, 후오존과 BAC 여과과정을 거치면서 100% 제거되어짐을 확인할 수 있었다. 6개의 세포주를 사용하여 바이러스별 감수성을 조사한 결과 폴리오바이러스는 BGMK 세포주, 콕사키바이러스는 Vero 및 BGMK 세포주가 다른 세포주에 비해 높은 감수성을 나타내었고, 반면 에코바이러스는 RD 세포주에서 가장 분리가 용이하였다.

• 대한수의학회지
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• 제47권4호
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• pp.425-428
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• 2007

From January to June 2006, 54 suckling pigs had been submitted in virology lab., College of Veterinary Medicine, Seoul National University. All pigs had suffered from various symptoms such as respiratory sign, enteric signs, neurologic signs, etc. Among 54 pigs, 24 pigs (44.4%) were positive for porcine hemagglutinating encephalomyelitis virus (HEV) through reverse transcription-nested polymerase chain reaction. According to this result, HEV infections seemed to be prevalent and widespread in Korean swine farms, and the infection is associated with respiratory signs and neurologic signs more than enteric signs. The HEV positive pigs showing respiratory signs were co-infected with viruses such as PRRSV, and PCV2, or bacteria such as Pasteurella spp. The single infection may subclinically have an influence on outbreak of other respiratory pathogens in suckling pigs.

• 한국동물위생학회지
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• 제34권2호
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• pp.125-131
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• 2011

The present study was aimed to investigate the prevalence of porcine group A rotavirus, group C rotavirus and calicivirus from the 46 pig farms located in Incheon area. Group A rotavirus was detected in 16 (5.3%) from 8 farms (17.4%), and group C rotavirus was determined in 17 samples (5.7%) from 6 farms (13.0%). Porcine calicivirus was also detected in fecal samples [11 samples (3.7%) from 2 farms (4.3%)]. Correlation analysis was carried out among porcine enteric viruses and clinical signs, herd size and temperature on the basis of these results. The occurrence of porcine group A rotavirus, group C rotavirus and calicivirus infections in Incheon area was not associated with season and temperature. Especially, group C rotavirus was also detected in the pigs without any clinical symptoms.

• 한국환경보건학회지
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• 제48권3호
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• pp.176-182
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• 2022

Background: The risk of imported infectious diseases has been increasing with the annual rise in the number of international travelers. Objectives: This study aims to analyze the distribution and characteristics of intestinal bacteria isolated in 2019 from residents of Chungcheongnam-do Province with experience of travelling overseas. Methods: Twenty-three former overseas travelers with diarrhea were analyzed to detect viruses and bacteria according to the Manual for Detection of Foodborne Pathogens at Outbreaks. Additionally, antibiotic susceptibility tests and 16s rRNA sequencing were performed. Results: Twenty-five strains of ten pathogens were isolated from 18 samples. Pathogenic E. coli was the most common at 57.7%, followed by Clostridium perfringens (15.4%), Campylobacter spp. (7.7%), and Salmonella spp. (7.7%). The serotype of Salmonella was confirmed as Salmonella Braenderup, II 9,46:g,[m],[s],t:[e,n,x]. Conclusions: It was confirmed that the major enteric bacterial pathogens isolated from overseas travelers in Chungcheongnam-do Province were pathogenic E. coli, as found in other studies. The study on Plesiomonas shigelloides is meaningful in that it is reported as a rare case of infection in Korea. Antibiotic resistance and 16s rRNA analysis were performed, which is expected to provide important basic data for the prevention of traveler's diarrhea.