• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.404-411
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• 2019

In this study, the HEC-HMS was applied to determine rainfall-runoff processes for the Gokgyuchun basin. Several sub-basins have large-scale reservoirs for agricultural needs and they store large amounts of initial runoff. Three infiltration methods were implemented to reflect the effect of initial loss by reservoirs: 'SCS-CN'(Scheme I), 'SCS-CN' with simple surface method(Scheme II), and 'Initial and Constant rate'(Scheme III). Modeling processes include incorporating three different methods for loss due to infiltration, Clark's UH model for transformation, exponential recession model for baseflow, and Muskingum model for channel routing. The parameters were calibrated using an optimization technique with trial and error method. Performance measures, such as NSE, RAR, and PBIAS, were adopted to aid in the calibration processes. The model performance for those methods was evaluated at Gangcheong station, which is the outlet of study site. Good accuracy in predicting runoff volume and peak flow, and peak time was obtained using the Scheme II and III, considering the initial loss, whereas Scheme I showed low reliability for storms. Scheme III did not show good matches between observed and simulated values for storms with multi peaks. Conclusively, Scheme II provided better results for both single and multi-peak storms. The results of this study can provide a useful tool for decision makers to determine master plans for regional flood control management.

• Molecules and Cells
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• v.41 no.12
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• pp.1061-1071
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• 2018

From Xenopus embryo studies, the BMP4/Smad1-targeted gene circuit is a key signaling pathway for specifying the cell fate between the ectoderm and neuro-ectoderm as well as the ventral and dorsal mesoderm. In this context, several BMP4/Smad1 target transcriptional factors have been identified as repressors of the neuro-ectoderm. However, none of these direct target transcription factors in this pathway, including GATA1b, Msx1 and Ventx1.1 have yet been proven as direct repressors of early neuro-ectodermal gene expression. In order to demonstrate that Ventx1.1 is a direct repressor of neuro-ectoderm genes, a genome-wide Xenopus ChIP-Seq of Ventx1.1 was performed. In this study, we demonstrated that Ventx1.1 bound to the Ventx1.1 response cis-acting element 1 and 2 (VRE1 and VRE2) on the promoter for zic3, which is a key early neuro-ectoderm gene, and this Ventx1.1 binding led to repression of zic3 transcription. Site-directed mutagenesis of VRE1 and VRE2 within zic3 promoter completely abolished the repression caused by Ventx1.1. In addition, we found both the positive and negative regulation of zic3 promoter activity by FoxD5b and Xcad2, respectively, and that these occur through the VREs and via modulation of Ventx1.1 levels. Taken together, the results demonstrate that the BMP4/Smad1 target gene, Ventx1.1, is a direct repressor of neuro-ectodermal gene zic3 during early Xenopus embryogenesis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.568-573
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• 2018

UAV(Unmanned Aerial Vehicle) is widely used in space information construction, agriculture, fisheries, weather observation, communication, and entertainment fields because they are cheaper and easier to operate than manned aircraft. In particular, UAV have attracted much attention due to the speed and cost of data acquisition in the field of spatial information construction. However, ortho image images produced using UAVs are distorted in buildings and forests. It is necessary to solve these problems in order to utilize the geospatial information field. In this study, fixed wing, rotary wing, vertical take off and landing type UAV were used to detect distortions of ortho image of UAV under various conditions, and various object areas such as construction site, urban area, and forest area were captured and analysed. Through the research, it was found that the redundancy of the unmanned aerial vehicle image is the biggest factor of the distortion phenomenon, and the higher the flight altitude, the less the distortion phenomenon. We also proposed a method to reduce distortion of orthoimage by lowering the resolution of original image using DTM (Digital Terrain Model) to improve distortion. Future high-quality unmanned aerial vehicles without distortions will contribute greatly to the application of UAV in the field of precision surveying.

• Journal of Korea Water Resources Association
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• v.51 no.12
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• pp.1207-1216
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• 2018

Discharge or water level predictions at tidally affected river reaches are currently still a great challenge in hydrological practices. This research aims to predict water level of the tide dominated site, Jamsu bridge in the Han River downstream. Physics-based hydrodynamic approaches are sometimes not applicable for water level prediction in such a tidal river due to uncertainty sources like rainfall forecasting data. In this study, TensorFlow deep learning framework was used to build a deep neural network based LSTM model and its applications. The LSTM model was trained based on 3 data sets having 10-min temporal resolution: Paldang dam release, Jamsu bridge water level, predicted tidal level for 6 years (2011~2016) and then predict the water level time series given the six lead times: 1, 3, 6, 9, 12, 24 hours. The optimal hyper-parameters of LSTM model were set up as follows: 6 hidden layers number, 0.01 learning rate, 3000 iterations. In addition, we changed the key parameter of LSTM model, sequence length, ranging from 1 to 6 hours to test its affect to prediction results. The LSTM model with the 1 hr sequence length led to the best performing prediction results for the all cases. In particular, it resulted in very accurate prediction: RMSE (0.065 cm) and NSE (0.99) for the 1 hr lead time prediction case. However, as the lead time became longer, the RMSE increased from 0.08 m (1 hr lead time) to 0.28 m (24 hrs lead time) and the NSE decreased from 0.99 (1 hr lead time) to 0.74 (24 hrs lead time), respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.279-285
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• 2019

Transverse vibrations of a ship's aft end and deckhouse are mainly induced by transverse exciting forces from the main engine. Resonance should be avoided in the initial design stages when there is a prediction of resonance between the main engine and transverse modes of the deckhouse. Estimates of frequencies for resonance avoidance are possible from the specifications of the main engine and propeller, but the inherent vibration frequency of the structure around the engine room is not easy to estimate due to the variation in the shape. Experience-oriented vibration design is also carried out, which results in many problems, such as process delay, over-injection of on-site personnel, and iterative performance of the design. For the flexible design of 8,600 TEU container vessels, this study addressed the resonance problem caused by the transverse vibration of the main engine when only the main engine was changed from 12 cylinders to 10 cylinders without modification of the hull structure layout. Efficient structural reinforcement design guidelines are presented for avoiding resonances with the main engine lateral vibration and the structure around the engine room. The guidelines are expected to be used as practical design guidelines at design sites.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.477-484
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• 2019

Several types of scavenger receptors, including the Collectin-Placenta 1 (CL-P1) receptorthat is present in mammals, are molecules that are expressed on the surfaces of endothelial cells, macrophages and smooth muscle cells. These molecules are cell surface glycoproteins that can be conjugated to oxidized low density lipoprotein (oxLDL). Among these molecules, the effect of quercetin on CL-P1 activation has been confirmed. Quercetin is known as an antioxidant that stops oxidation because it acts to remove free radicals that are responsible for the oxidation reaction. In this study, fragments from the transcription start site of the mouse CL-P1 gene promoter to the -500th base were cloned using DNA polymerase. These fragments were then introduced into macrophage like RAW264.7 cells and fibroblast-like NIH3T3 cells to study the effect of quercetin on the CL-P1 gene expression. As a result, we found that bases ranging from -250 to -350 in the anterior part where gene expression starts are important for producing CL-P1 protein. Among them, the DNA mutation experiments we performed confirmed that the E2F binding sites are critical for producing the CL-P1 protein? In addition, when quercetin was added to the RAW264.7 culture medium, which was a culture of adherent cells, observedthe phenomenon of the cells falling off from the surface of the culture container.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.6
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• pp.439-447
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• 2019

In this study, experiments were conducted to establish appropriate measures for slopes with high risk of collapse and to obtain results for minimizing slope collapse damage by detecting the micro-displacement of soil in advance by installing a laser sensor and a vibration sensor in the landslide reduction model experiment. Also, the behavior characteristics of the soil layer due to rainfall and moisture ratio changes such as pore water pressure and moisture were analyzed through a landslide reduction model experiment. The artificial slope was created using granite weathering soil, and the resulting water ratio(water pressure, water) changes were measured at different rainfall conditions of 200mm/hr and 400mm/hr. Laser sensors and vibration sensors were applied to analyze the surface displacement, and the displacement time were compared with each other by video analysis. Experiments have shown that higher rainfall intensity takes shorter time to reach the limit, and increase in the pore water pressure takes shorter time as well. Although the landslide model test does not fully reflect the site conditions, measurements of the time of detection of displacement generation using vibration sensors show that the timing of collapse is faster than the method using laser sensors. If ground displacement measurements using sensors are continuously carried out in preparation for landslides, it is considered highly likely to be utilized as basic data for predicting slope collapse, reducing damage, and activating the measurement industry.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.352-358
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• 2018

This study aimed to develop a species identification method for the egg and fry of the three Korean bitterling fishes (Pisces: Acheilognathinae), including Acheilognathus signifer, Acheilognathus yamatsutae and Rhodeus uyekii based on the PCR-based Restriction Fragment Length Polymorphism (RFLP) markers. We conducted a field survey on the Deokchicheon River from the North Han River basin, where the three Acheilognathinae species co-occur, and also analyzed the existing sequence dataset available from the GenBank. We found coexistence of the three species at the study site. The egg and fry were obtained from the host mussels (Unio douglasiae sinuolatus) by hand from May to June 2015 and in May 2017. To develop PCR-based RFLP markers for species identification of the three Acheilognathinae fish species, restriction enzymes pinpointing species-specific single nucleotide variation (SNV) sites in mitochondrial DNA COI (cytochrome oxidase I) and cyt b (cytochrome b) genes were determined. Genomic DNA was extracted from the egg and fry and RFLP experiments were carried out using restriction enzymes Apal I, Stu I and EcoR V for A. signifer, A. yamatsutae and R. uyekii, respectively. Consequently, unambiguous discrimination of the three species was possible, as could be seen in DNA band patterns from gel electrophoresis. Our developed PCR-based RFLP markers will be useful for the determination of the three species for the young and would assist in studying the spawning patterns and reproductive ecology of Acheilognathinae fishes. Furthermore, we believe the obtained information will be of importance for future maintenance, management and conservation of these natural and endangered species.

• Journal of the Korean Recycled Construction Resources Institute
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• v.7 no.1
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• pp.16-21
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• 2019

This study has its aim to judge both applicability and suitability of recycling of waste soil for the use of farmland amelioration and low-lying farmland reclamation through growth and development experiment and component analysis. As results of physical characteristic evaluation on recycling of waste soil, the classification based on unified soil classification system has investigated as SW and SP affiliation and soil classification has appeared to be a loamy sand. As results of chemical component analysis, pH has appeared to be 7.0~8.4 which is relatively higher than general soil, however, heavy metal has investigated within the 1 region's standard value of soil pollution standards. As results of germination experiment, when using it by mixing recycling soil less than 75%, there is no significant influence on germination, and in the growth and development experiment, when using horticultural bed soil which is mixed with less than 40% of recycling of waste soil, it has confirmed that there is no significance difference with general soil. In case of farmland, the growth disorder of recycling of waste soil rate no more than 40% has shown that it has relatively small influences, and in case of using it by mixing with agricultural soil, it has evaluated to require concrete review of factors which may restrict growth condition including nutrition and pH.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.5
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• pp.502-509
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• 2019

Studies have been made on performance evaluation of expansion joint systems for an ordinary highway or road bridge. However little study has been made for runway connection bridges at airports. A study on performance evaluated from computer code analysis and shrinkage, extension, and compression repetition tests based on KS F 4425 is conducted to a newly developed expansion joint system which has been installed in a runway connection bridge at Incheon Airport Extension 2 Construction Site. The MIDAS computer code is used to analyze the performance before the manufacture of the mock-up of expansion joint system on the basis of design requirements. Tests based on the KS F 4425 of 2001 year-version are conducted for the mock-up. Domestic codes and standards to validate the performance of the expansion joint system in a connection bridge have been developed for a vehicle. However the expansion joint system tested in this study is installed in a runway connection bridge for an aircraft. Conservatively the heaviest one among airplanes departing and landing at Incheon Airport is assumed level-F $468.4kN/m^2$ and adopted for the tests and analyses in this study. KS F 4425 method is selected for the shrinkage, extension, and compression repetition tests. No remarkable problem was observed for the 2,500-cycle shrinkage and extension and two million-cycle repeatition load tests. The results of this study are expected to contribute to establishment of code and standard for the performance validation of an expansion joint system installed in a runway connection bridge for an aircraft by providing performance test results and computer analysis results based on finite element methods.