• Journal of Life Science
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• v.23 no.5
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• pp.676-681
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• 2013

Perchlorate (${ClO_4}^-$) is an emerging contaminant found in surface water and soil/groundwater. Microbial removal of perchlorate is the method of choice since perchlorate-reducing bacteria (PRB) can reduce perchlorate to harmless end-products. A previous study [3] showed experimental evidence of autotrophic perchlorate removal using elemental sulfur granules and activated sludge. The granular sulfur is a relatively inexpensive electron donor, and activated sludge is easily available from a wastewater treatment plant. A batch test was performed in this study to further investigate the effect of various environmental parameters on the perchlorate degradation by sludge microorganisms when elemental sulfur was used as electron donor. Results of the batch test suggest optimum conditions for autotrophic perchlorate degradation by sludge microorganisms. The results also show that sulfur-oxidizing PRB enriched from activated sludge removed perchlorate better than activated sludge. Taken together, this study suggests that autotrophic perchlorate removal using elemental sulfur and activated sludge can be improved by employing optimized environmental conditions and enrichment culture.

• Molecules and Cells
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• v.38 no.4
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• pp.318-326
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• 2015

We previously reported that the SbROMT3syn recombinant protein catalyzes the production of the methylated resveratrol derivatives pinostilbene and pterostilbene by methylating substrate resveratrol in recombinant E. coli. To further study the production of stilbene compounds in E. coli by the expression of enzymes involved in stilbene biosynthesis, we isolated three stilbene synthase (STS) genes from rhubarb, peanut, and grape as well as two resveratrol O-methyltransferase (ROMT) genes from grape and sorghum. The ability of RpSTS to produce resveratrol in recombinant E. coli was compared with other AhSTS and VrSTS genes. Out of three STS, only AhSTS was able to produce resveratrol from p-coumaric acid. Thus, to improve the solubility of RpSTS, VrROMT, and SbROMT3 in E. coli, we synthesized the RpSTS, VrROMT and SbROMT3 genes following codon-optimization and expressed one or both genes together with the cinnamate/4-coumarate:coenzyme A ligase (CCL) gene from Streptomyces coelicolor. Our HPLC and LC-MS analyses showed that recombinant E. coli expressing both ScCCL and RpSTSsyn led to the production of resveratrol when p-coumaric acid was used as the precursor. In addition, incorporation of SbROMT3syn in recombinant E. coli cells produced resveratrol and its mono-methylated derivative, pinostilbene, as the major products from p-coumaric acid. However, very small amounts of pterostilbene were only detectable in the recombinant E. coli cells expressing the ScCCL, RpSTSsyn and SbROMT3syn genes. These results suggest that RpSTSsyn exhibits an enhanced enzyme activity to produce resveratrol and SbROMT3syn catalyzes the methylation of resveratrol to produce pinostilbene in E. coli cells.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.176-183
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• 2008

Recently, several large-scale Mega-Projects are being conducted. For these urban revitalization projects which requires many complex functions, the existing project management system based on single project level is limited in application. Therefore, our main objectives of this research are two 1) Develop a brand-new program management system(Prototype Ver 1.0) for mega-projects where various facilities are combined both horizontally and vertically. 2) Develop management strategies(Prototype Ver 1.0) based on the program level that enable the comprehensive management of a multiple various projects. The subtitles of this Research are i-PMIS(Program Management Information System) Development, Standardization & Optimization of Construction Life-Cycle Process, Comprehensive Project Cost & Process Management Technology, Effective and Optimized Integrated Performance Management Technology, and, we suggest to optimize the whole life cycle process, predict and respond to various risks, predict and control the process, the cost and the schedule, achieve maximum return on investment to the participating parties, and provide a brand-new Program-MIS including the visual-based web-portal platform to respond the changing business environments and decision making.

• Journal of Biomedical Engineering Research
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• v.25 no.1
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• pp.57-64
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• 2004

The non pulsation blood pump is divided into axial flow and centrifugal style according to the direction of inlet and outlet flow. An axial flow blood pump can be made smaller than a centrifugal blood pump because centrifugal pump's rpm is fewer than axial flow pump. Hemolysis is an important factor for the development of an axial flow blood pump. It is difficult to identify the areas where hemolysis occurs. Evaluation of hemolysis both in in-vitro and in-vivo test requires a long-time and more expensive. Computational fluid dynamics(CFD) analysis enables the engineer to predict hemolysis on a computer which just can get not only amount of htmolysis but also location of hemolysis. It takes shorter time and less expensive than in-vitro test. The purpose of this study is to git Computational fluid dynamics in axial flow pump and to verify the accuracy of prediction by the possibility of design comparing CFD results with in-vitro experimental results. Also, wish to figure out the correction method that can bring improvement in shape of axial flow blood pump using CFD analysis.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.42 no.1
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• pp.1-11
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• 2005

Several Solid Freeform Fabrication Systems(SFFS) are commercialized in a few companies for rapid prototyping. However, they have many technical problems including the limitation of applicable materials. A new method of agile prototyping is required for the recent manufacturing environments of multi-item and small quantity production. The objectives of this paper include the development of a novel method of SFFS, the CAFL/sup VM/(Computer Aided Fabrication of Lamination for Various Material), and the manufacture of the various material samples for the certification of the proposed system and the creation of new application areas. For these objectives, the technologies for a highly accurate robot path control, the optimization of support structure, CAD modeling, adaptive slicing was implemented. However, there is an important problem with the conventional 2D lamination method. That is the inaccuracy of 3D model surface, which is caused by the stair-type surface generated in virtue of vertical 2D cutting. In this paper, We design the new control algorithm that guarantees the constant speed, precise positioning and tangential cutting on the 5DOF SFFS. We develop the tangential cutting algorithm to be controlled with constant speed and successfully implemented in the 5DOF CAFL/sup VM/ system developed in this paper. Finally, this paper confirms its high-performance through the experimental results from the application into CAFL/sup VM/ system.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.45 no.1
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• pp.37-47
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• 2008

Exploitation of address generation units which are typically provided in DSPs plays an important role in DSP code generation since that perform fast address computation in parallel to the central data path. Offset assignment is optimization of memory layout for program variables by taking advantage of the capabilities of address generation units, consists of memory layout generation and address pointer assignment steps. In this paper, we propose an effective address pointer assignment method to minimize the number of address calculation instructions in DSP code generation. The proposed approach reduces the time complexity of a conventional address pointer assignment algorithm with fixed memory layouts by using minimum cost-nodes breaking. In order to contract memory size and processing time, we employ a powerful pruning technique. Moreover our proposed approach improves the initial solution iteratively by changing the memory layout for each iteration because the memory layout affects the result of the address pointer assignment algorithm. We applied the proposed approach to about 3,000 sequences of the OffsetStone benchmarks to demonstrate the effectiveness of the our approach. Experimental results with benchmarks show an average improvement of 25.9% in the address codes over previous works.

• Korean Chemical Engineering Research
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• v.55 no.2
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• pp.141-155
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• 2017

In the present paper, we investigated the development status of precipitated calcium carbonate (PCC) production using steel slag, which is one of mineral carbonation (MC) technologies, from the standpoint of $CO_2$ utilization. Principle, feature, and global and domestic development status of the mineral carbonation technology were discussed together with the overview of the production method and market of PCC. Mineral carbonation is known as stable and environmentally-friendly technology enabling economical treatment of industrials wastes. Typically, PCC is produced by the reaction of $CO_2$ with supernatant solution after Ca extraction from steel slag followed by the separation of solid and liquid. The development status of MC using steel slag is at the pilot stage (Slag2PCC at Aalto University), and there remains the process economics improvement for commercialization. Key technologies for the further development are efficient extraction of Ca ions from steel slag including impurities removal, valorization of PCC via shape and size control, usage development and value-addition of residual slag, and optimization of reaction conditions for continuous process setup, etc.

• Korean Chemical Engineering Research
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• v.56 no.5
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• pp.647-654
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• 2018

In order to solve the global warming and reduce greenhouse gas emissions, it has been developed the $CO_2$ capture technology by oxy-fuel combustion. But there is a problem that the economic efficiency is low because the oxygen production cost is high. ASU (Air Separation Unit) is known to be most suitable method for producing large capacity of oxygen (>2,000 tpd). But most of them are optimized for high purity (>99.5%) oxygen production. If the ASU process is optimized for low purity(90~97%) oxygen producing, it is possible to reduce the production cost of oxygen by improving the process efficiency. In this study, the process analysis and comparative evaluation was conducted for developing large capacity ASU for oxy-fuel combustion. The process efficiency was evaluated by calculating the recovery rate and power consumption according to the oxygen purity using the AspenHysys. As a result, it confirmed that the optimal purity of oxygen for oxyfuel combustion is 95%, and the power consumption can be reduced by process optimization to 12~18%.

• Korean Chemical Engineering Research
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• v.46 no.1
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• pp.194-199
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• 2008

In this study, the effective method to esterify the free fatty acids in jatropha oil was examined. Compared to other plant oils, the acid value of jatropha oil was remarkably high, 11.5 mgKOH/g. So direct transesterification by a base catalyst was not suitable for the oil. After the free fatty acids were esterified with methanol, jatropha oil was transesterified. The activities of four solid acid catalysts were tested and Amberlyst-15 showed the best activity for the esterification. After constructing the experiment matrix based on RSM and analyzing the statistical data, the optimal esterification conditions were determined to be 6.79% of methanol and 17.14% of Amberlyst-15. After the pretreatment, jatropha biodiesel was produced by the transesterification using KOH in a pressurized batch reactor. Jatropha biodiesel produced could meet the major specifications of Korean biodiesel standards; 97.35% of FAME, 8.17 h of oxidation stability, 0.125% of total glycerol and $0^{\circ}C$ of CFPP.

• Journal of the Earthquake Engineering Society of Korea
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• v.11 no.3 s.55
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• pp.1-10
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• 2007

In general, the tension forces of hanger cable in suspension bridges play an important role in evaluating the bridge conditions. The vibration method, as a conventional one, has been widely applied to estimate the tension forces by using the measured frequencies on hanger cables. However, the vibration method is not applicable to short hanger cables because the fiequencies of short cables are severely sensitive to flexural rigidity. Thus, in this study, the tension forces of short hanger cables, of which the length is shorter than 10 meters, were estimated through back analysis of the cable fiequencies measured from Gwang-An suspension bridge in Korea. Direct approach to back analysis is adopted using the univariate method among the direct search methods as an optimization technique. The univariate method is able to search the optimal tension forces without regard to the initial ones and has a rapid convergence rate. To verify the feasibility of back analysis, the results from back analysis and vibration method are compared with the design tension forces. From the comparison, it can be inferred that back analysis results are more reasonable agreement with the design tension forces of short hanger cable. Therefore, it is concluded that back analysis applied in this study is an appropriate tool for estimating tension forces of short hanger cables.