• Title/Summary/Keyword: engineered surface

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Electrochemical Synthesis of Dumbbell-like Au-Ni-Au Nanorods and Their Surface Plasmon Resonance

  • Park, Yeon Ju;Liu, Lichun;Yoo, Sang-Hoon;Park, Sungho
    • Journal of Electrochemical Science and Technology
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    • v.3 no.2
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    • pp.57-62
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    • 2012
  • In this report, we demonstrate that the longitudinal localized surface plasmon resonance mode can be suppressed when the nanorods were in dumbbell shape. The seed nanorods were synthesized by electrochemical deposition of metals into the pores of anodic aluminum oxide templates. The dumbbell-like nanorods were grown from seed Au-Ni-Au nanorods by a rate-controlled seed-mediated growth strategy. The selective deposition of Au atoms onto Au blocks of Au-Ni-Au nanorods produced larger diameter of Au nanorods with bumpy surface resulting in dumbbell-like nanorods. The morphology of nanorods depended on the reduction rate of $AuCl_4^-$, slow rate producing smooth surface of Au nanorods, but high reduction rate producing bumpy surface morphology. Through systematic investigation into the UV-Vis-NIR spectroscopy, we found that the multiple localized surface plasmon resonance (LSPR) modes were available from single-component Au nanorods. And, their LSPR modes of Au NRs with bumpy surface, compared to the smooth seed Au NRs, were red-shifted, which was obviously attributed to the increased electron oscillation pathways. While the longitudinal LSPR modes of smoothly grown Au NRs were blue-shifted except for a dipole transverse LSPR mode, which can be interpreted by decreased aspect ratio. In addition, dumbbell-like nanorods showed an almost disappeared longitudinal LSPR mode. It reflects that the plasmonic properties can be engineered using complex nanorods structure.

The dependence of contractile force for the cardiomyocytes on a different engineered surface (바닥면상태에 따른 심장세포의 수축력 변화)

  • Kim, Jin-Seok;Park, Jung-Yul;Ryu, Suk-Kyu;Baek, Jeong-Eun;Cha, Jung-Hun;Park, Se-Wan;Kim, Hyeon-Chul;Kim, Byung-Kyu;Park, Suk-Ho;Chun, Kuk-Jin
    • Proceedings of the IEEK Conference
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    • 2005.11a
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    • pp.881-884
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    • 2005
  • We present a microfabricated three-dimensional (3-D) hybrid biopolymer micro cantilever which can measure the contractile force of cardiomyocytes and analyze the force dependence on different types of surface. To make different conditions of the cell seeding surface, we fabricated the specific type of cantilever which was grooved on the surface. The presented cantilever was facilitated to measure bending of the cantilever and to calculate the contractile force of cardiomyocytes. Also, we demonstrate the dependence of the morphology for the cardiomyocytes seeded on a plain surface and a grooved surface. Finally, we show the dependence of force for the cardiomyocytes on a different surface.

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Modeling Technology on Free-form Surface of a New Military Personal Head using Quick Surface Method (퀵서피스기법을 이용한 신장병 두상의 자유곡면 모델링 기술)

  • Lee, Yong-Moon;Hwang, Tae-Son;Kim, Hun;Nam, Hee-Tae;Lee, Kee-Hwan;Kang, Myungchang
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.17 no.6
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    • pp.170-176
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    • 2018
  • Recently, weapon system requires personal protection products due to the explosion of rapid-fire explosion, which is considered to be multi threat in modernization, complication and war against terrorism. However, the conventional Korean military bullet protection helmets are not suitable for wearing convenience and combatant interoperability in terms of ergonomic. In this paper, we propose a suitable 3D Scanning method for the head, and compare the measured 3D dimension with the existing 2D measurement value to identity the reliability. Reverse engineered soldier head using the quick surface method was realized with a perfect free-form surface and satisfactory tolerance range (${\pm}0.2mm$). Through the comparison of 3D and 2D measured head dimensions, the absolute error value was 0.73 mm on average and relative error was 0.35 %, confirming the high accuracy of the 3D scan modeling. Also, quick surface method using 3D scanner is suggested a fast and accurate skill for ergonomics in obtaining the head modeling needed for military's personal bullet protection helmet design.

Growth and Migration of BALB/3T3 Fibroblast Cells on Nano-engineered Silica Beads Surface

  • Kim, Jihee;Chandra, Prakash;Yang, Jiyoon;Rhee, Seog Woo
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3715-3721
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    • 2013
  • In this study, the behavior of cells on the modified surface, and the correlation between the modified substrates and the response of cells is described. A close-packed layer of nano-sized silica beads was prepared on a coverslip, and the adhesion, proliferation, and migration of BALB/3T3 fibroblast cells on the silica layer was monitered. The 550 nm silica beads were synthesized by the hydrolysis and condensation reaction of tetraethylorthosilicate in basic solution. The amine groups were introduced onto the surfaces of silica particles by treatment with 3-aminopropyltrimethoxysilane. The close-packed layer of silica beads on the coverslip was obtained by the reaction of the amine-functionalized silica beads and the (3-triethoxysilyl)propylsuccinic anhydride treated coverslip. BALB/3T3 fibroblast cells were loaded on bare glass, APTMS coated glass, and silica bead coated glass with the same initial cell density, and the migration and proliferation of cells on the substrates was investigated. The cells were fixed and stained with antibodies in order to analyze the changes in the actin filaments and nuclei after culture on the different surfaces. The motility of cells on the silica bead coated glass was greater than that of the cells cultured on the control substrate. The growth rate of cells on the silica bead coated glass was slower than that of the control. Because the close-packed layer of silica beads gave an embossed surface, the adhesion of cells was very weak compared to the smooth surfaces. These results indicate that the adhesion of cells on the substrates is very important, and the actin filaments might play key roles in the migration and proliferation of cells. The nuclei of the cells were shrunk on the weakly adhered surfaces, and the S1 stage in which DNA is duplicated in the cell dividing processes might be retarded. As a result, the rate of proliferation of cells was decreased compared to the smooth surface of the control. In conclusion, the results described here are very important in the understanding of the interaction between implanted materials and biosystems.

A Study on the Anti-Spalling Performance of High-Strength Concrete Members by covered Engineered Cementitious Composite (ECC로 피복된 고강도콘크리트 부재의 폭렬억제성능에 관한 연구)

  • Lee, Jae-Young;Kim, Jae-Hwan;Han, Byung-Chan;Park, Sun-Gyu;Kwon, Young-Jin
    • Fire Science and Engineering
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    • v.22 no.4
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    • pp.85-94
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    • 2008
  • The purpose of this study is to obtain the fundamental fire resistance performance of engineered cementitious composites (ECC) under fire temperature in order to use the fire protection material in high-strength concrete structures. The present study conducted the experiment to simulate fire temperature by employing of ECC and investigated experimentally the explosion and cracks in heated surface of these ECC. In the experimental studies, 5 HSC specimens are being exposed to fire, in order to exami ne the influence of vari ous parameters (such as depth of layer=20, 30, 40 mm; construction method=lining and repairing type) on the fire performance of HSC structures. Employed temperature curve were ISO 834 criterion (3 hr), which are severe in various criterion of fire temperature in building structures. The numerical regressive analysis and proposed equation to calculate ambient temperature distribution is carried out and verified against the experimental data. By the use of proposed equation, the HSC members subjected to fire loads were designed and discussed.

Magnetotelluric modeling considering vertical transversely isotropic electrical anisotropy (수직 횡등방성 전기적 이방성을 고려한 자기지전류탐사 모델링)

  • Kim, Bitnarae;Nam, Myung Jin
    • Geophysics and Geophysical Exploration
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    • v.18 no.4
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    • pp.232-240
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    • 2015
  • Magnetotelluric (MT) survey investigates electrical structure of subsurface by measuring natural electromagnetic fields on the earth surface. For the accurate interpretation of MT data, the precise three-dimensional (3-D) modeling algorithm is prerequisite. Since MT responses are affected by electrical anisotropy of medium, the modeling algorithm has to incorporate the electrical anisotropy especially when analyzing time-lapse MT data sets, for monitoring engineered geothermal system (EGS) reservoir, because changes in different-vintage MT-data sets are small. This study developed a MT modeling algorithm for the simulation MT responses in the presence of electrical anisotropy by improving a pre-existing staggered-grid finite-difference MT modeling algorithm. After verifying the developed algorithm, we analyzed the effect of vertical transversely isotropic (VTI) anisotropy on MT responses. In addition, we are planning to extend the applicability of the developed algorithm which can simulate not only the horizontal transversely isotropic (HTI) anisotropy, but also the tiled transversely isotropic (TTI) anisotropy.

Performance Assessment of Low- and Intermediate-Level Radioactive Waste Disposal Facility in Korea by Using Complementary Indicator: Case Study with Radionuclide Flux (보조지표를 활용한 중·저준위 처분시설 성능평가: 방사성 핵종 플럭스 사례연구)

  • Jung, Kang-Il;Jeong, Mi-Seon;Park, Jin Beak
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.13 no.1
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    • pp.73-86
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    • 2015
  • The use of complimentary indicators, other than radiation dose and risk, to assess the safety of radioactive waste disposal has been discussed in a number of publications for providing the reasonable assurance of disposal safety and convincing the public audience. In this study, the radionuclide flux was selected as performance indicator to appraise the performance of engineered barriers and natural barrier in the Wolsong low- and intermediate-level waste disposal facility. Radionuclide flux showing the retention capability by each compartment of the disposal system is independent of assumptions in biosphere model and exposure pathways. The scenario considered as the normal scenario of disposal facility has been divided into intact or degraded silo concrete conditions. In the intact silo concrete, the radionuclide flux has been assessed with respect to the radionuclide retardation performance of each engineered barrier. In the degraded silo concrete, the radionuclide flux has been explored based on the performance degradation of engineered barriers and the relative significance of natural barrier quantitatively. The results can be used to optimally design the near-surface disposal facility being planned as the second project phase. In the future, additional complimentary indicators will be employed for strengthening the safety case for improving the public acceptance of low- and intermediate-level waste disposal facility.

An Evaluation of Soil-Water Characteristic Curve Model for Compacted Bentonite Considering Temperature Variation (온도 변화를 고려한 압축 벤토나이트 완충재의 함수특성곡선 모델 평가)

  • Yoon, Seok;Jeon, Jun-Seo;Go, Gyu-Hyun;Kim, Geon-Young
    • Journal of the Korean Geotechnical Society
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    • v.36 no.10
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    • pp.33-39
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    • 2020
  • A geological repository has been considered as an option for the disposal of high-level radioactive waste (HLW). The HLW is disposed in a host rock at a depth of 500~1,000 meters below the ground surface based on the concept of engineered barrier system (EBS). The EBS is composed of a disposal canister, buffer material, backfill material, and gap-filling material. The compacted bentonite buffer is very important since it can restrain the release of radionuclide and protect the canister from the inflow of ground water. The saturation of the buffer decreases because high temperature in a disposal canister is released into the surrounding buffer material, but saturation of the buffer increases because of the inflow of ground water. The unsaturated properties of the buffer are critical input parameters for the entire safety assessment of the engineered barrier system. In Korea, Gyeongju bentonite can be considered as a candidate buffer material, but there are few test results of the unsaturated properties considering temperature variation. Therefore, this paper conducted experiment of soil-water characteristic curve for the Gyeongju compacted bentonite considering temperature variation under a constant water content condition. The relative error showed approximately 2% between test results and modified van-Genuchten model values.

Techniques for dental implant nanosurface modifications

  • Pachauri, Preeti;Bathala, Lakshmana Rao;Sangur, Rajashekar
    • The Journal of Advanced Prosthodontics
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    • v.6 no.6
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    • pp.498-504
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    • 2014
  • PURPOSE. Dental implant has gained clinical success over last decade with the major drawback related to osseointegration as properties of metal (Titanium) are different from human bone. Currently implant procedures include endosseous type of dental implants with nanoscale surface characteristics. The objective of this review article is to summarize the role of nanotopography on titanium dental implant surfaces in order to improve osseointegration and various techniques that can generate nanoscale topographic features to titanium implants. MATERIALS AND METHODS. A systematic electronic search of English language peer reviewed dental literature was performed for articles published between December 1987 to January 2012. Search was conducted in Medline, PubMed and Google scholar supplemented by hand searching of selected journals. 101 articles were assigned to full text analysis. Articles were selected according to inclusion and exclusion criterion. All articles were screened according to inclusion standard. 39 articles were included in the analysis. RESULTS. Out of 39 studies, seven studies demonstrated that bone implant contact increases with increase in surface roughness. Five studies showed comparative evaluation of techniques producing microtopography and nanotopography. Eight studies concluded that osteoblasts preferably adhere to nano structure as compared to smooth surface. Six studies illustrated that nanotopography modify implant surface and their properties. Thirteen studies described techniques to produce nano roughness. CONCLUSION. Modification of dental osseous implants at nanoscale level produced by various techniques can alter biological responses that may improve osseointegration and dental implant procedures.

Mucosal Administration of Lactobacillus casei Surface-Displayed HA1 Induces Protective Immune Responses against Avian Influenza A Virus in Mice

  • Dung T. Huynh;W.A. Gayan Chathuranga;Kiramage Chathuranga;Jong-Soo Lee;Chul-Joong Kim
    • Journal of Microbiology and Biotechnology
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    • v.34 no.3
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    • pp.735-745
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    • 2024
  • Avian influenza is a serious threat to both public health and the poultry industry worldwide. This respiratory virus can be combated by eliciting robust immune responses at the site of infection through mucosal immunization. Recombinant probiotics, specifically lactic acid bacteria, are safe and effective carriers for mucosal vaccines. In this study, we engineered recombinant fusion protein by fusing the hemagglutinin 1 (HA1) subunit of the A/Aquatic bird/Korea/W81/2005 (H5N2) with the Bacillus subtilis poly γ-glutamic acid synthetase A (pgsA) at the surface of Lactobacillus casei (pgsA-HA1/L. casei). Using subcellular fractionation and flow cytometry we confirmed the surface localization of this fusion protein. Mucosal administration of pgsA-HA1/L. casei in mice resulted in significant levels of HA1-specific serum IgG, mucosal IgA and neutralizing antibodies against the H5N2 virus. Additionally, pgsA-HA1/L. casei-induced systemic and local cell-mediated immune responses specific to HA1, as evidenced by an increased number of IFN-γ and IL-4 secreting cells in the spleens and higher levels of IL-4 in the local lymphocyte supernatants. Finally, mice inoculated with pgsA-HA1/L. casei were protected against a 10LD50 dose of the homologous mouse-adapted H5N2 virus. These results suggest that mucosal immunization with L. casei displaying HA1 on its surface could be a potential strategy for developing a mucosal vaccine against other H5 subtype viruses.