• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1083-1095
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• 2019

Butyrate is known to play a significant role in energy metabolism and regulating genomic activities that influence rumen nutrition utilization and function. Thus, this study investigated the effects of an isolated butyrate-producing bacteria, Clostridium saccharobutylicum, in rumen butyrate production, fermentation parameters and microbial population in Holstein-Friesian cow. An isolated butyrate-producing bacterium from the ruminal fluid of a Holstein-Friesian cow was identified and characterized as Clostridium saccharobutylicum RNAL841125 using 16S rRNA gene sequencing and phylogenetic analyses. The bacterium was evaluated on its effects as supplement on in vitro rumen fermentation and microbial population. Supplementation with $10^6CFU/ml$ Clostridium saccharobutylicum increased (p < 0.05) microbial crude protein, butyrate and total volatile fatty acids concentration but had no significant effect on $NH_3-N$ at 24 h incubation. Butyrate and total VFA concentrations were higher (p < 0.05) in supplementation with $10^6CFU/ml$ Clostridium saccharobutylicum compared with control, with no differences observed for total gas production, $NH_3-N$ and propionate concentration. However, as the inclusion rate (CFU/ml) of C. saccharobutylicum was increased, reduction of rumen fermentation values was observed. Furthermore, butyrate-producing bacteria and Fibrobacter succinogenes population in the rumen increased in response with supplementation of C. saccharobutylicum, while no differences in the population in total bacteria, protozoa and fungi were observed among treatments. Overall, our study suggests that supplementation with $10^6CFU/ml$ C. saccharobutylicum has the potential to improve ruminal fermentation through increased concentrations of butyrate and total volatile fatty acid, and enhanced population of butyrate-producing bacteria and cellulolytic bacteria F. succinogenes.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제8권6호
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• pp.243-250
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• 2019

움직이는 UAV는 많은 위치에너지와 운동에너지를 가지므로 지상으로 추락하는 경우 많은 충격량을 가질 수 있다. 이는 인명피해로 연결될 수 있기 때문에 본 논문에서는 UAV 비행경로 상의 인구밀집지역을 위험구역으로 정의하였다. 기존의 UAV 경로비행은 사용자에 의해 미리 설정된 경로만을 운행하는 수동적인 형태였다. 일부 UAV는 경로비행 중 장애물을 회피하는 시스템 등 안전기능을 포함하고 있지만, 실시간 비행환경변화에 대응하기에는 부족하다. UAV 경로비행에 공공 빅데이터를 활용할 경우, 위험구역을 검출하고 회피비행을 수행할 수 있어서 실시간 비행환경변화에 대한 대응이 향상될 수 있다. 따라서 본 논문에서는 실시간으로 수집된 빅데이터를 활용하여 위험구역을 회피하는 최적경로 비행 방안을 제안한다. 실험 결과, 제안하는 자동경로비행에서 목적지와 목적지에 따른 경로를 지정할 경우, 실시간으로 위험지역을 판단하여 최적 우회경로로 비행하는 것을 확인하였다. 추후 회피방안에 따라 비행하여 획득하는 영상의 질적 만족도를 높일 수 있는 방안을 연구할 예정이다.

• Parasites, Hosts and Diseases
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• 제57권4호
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• pp.341-357
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• 2019

Acanthamoeba, one of free-living amoebae (FLA), remains a high risk of direct contact with this protozoan parasite which is ubiquitous in nature and man-made environment. This pathogenic FLA can cause sight-threatening amoebic keratitis (AK) and fatal granulomatous amoebic encephalitis (GAE) though these cases may not commonly be reported in our clinical settings. Acanthamoeba has been detected from different environmental sources namely; soil, water, hotspring, swimming pool, air-conditioner, or contact lens storage cases. The identification of Acanthamoeba is based on morphological appearance and molecular techniques using PCR and DNA sequencing for clinico-epidemiological purposes. Recent treatments have long been ineffective against Acanthamoeba cyst, novel anti-Acanthamoeba agents have therefore been extensively investigated. There are efforts to utilize synthetic chemicals, lead compounds from medicinal plant extracts, and animal products to combat Acanthamoeba infection. Applied nanotechnology, an advanced technology, has shown to enhance the anti-Acanthamoeba activity in the encapsulated nanoparticles leading to new therapeutic options. This review attempts to provide an overview of the available data and studies on the occurrence of pathogenic Acanthamoeba among the Association of Southeast Asian Nations (ASEAN) members with the aim of identifying some potential contributing factors such as distribution, demographic profile of the patients, possible source of the parasite, mode of transmission and treatment. Further, this review attempts to provide future direction for prevention and control of the Acanthamoeba infection.

• 생명과학회지
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• 제28권12호
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• pp.1469-1476
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• 2018

Warburg 효과의 발생은 고형암에서 화학적 항암제의 내성을 발생시킨다. 따라서 호기성 해당과정과 같은 에너지 대사과정은 암 치료의 중요한 표적으로 알려져 있다. Pyruvate dehydrogenase kinase (PDK) 활성 억제물질로 알려진 dichloroacetate (DCA)는 많은 암세포에서 포도당의 호기성 해당과정을 산화적인산화 과정으로 전환시킬 수 있음이 보고되었다. 이 연구는 치료가 매우 어렵다고 알려진 인간 역분화 갑상선 암세포주인 8505C의 성장에 미치는 DCA효과를 조사하였다. DCA는 정상 갑상선 세포주의 성장에는 영향을 주지 않은 반면 8505C 세포주의 성장을 특이적으로 저해하였다. DCA의 처리에 의해 8505C 세포주는 $HIF1{\alpha}$, PDK1, Bcl-2와 같은 항-세포자살 관련 단백질들의 발현이 감소하고, Bax와 p21과 같은 세포자살 유도 단백질과 세포주기 억제 단백질의 증가로 인하여 세포주기 정지와 세포자살 유도에 의해 성장이 억제되었다. 이런 세포의 변화는 DCA 처리에 의한 활성산소족의 생산이 증가하고, 포도당 대사가 호기성 해당과정에서 산화적인산화 과정으로 전환되었기 때문이란 것을 확인하였다. 흥미롭게도, DCA는 포도당 대사과정의 변화뿐만 아니라 sodium/iodine symporter (NIS)의 발현양도 증가시킨다는 것을 확인하였다. 이 연구의 결과로 PDK 활성 저해제는 치료하기 힘든 역분화 갑상선 암 치료제로 이용할 수 있고, 또한 역분화 갑상선 암에 대한 방사능 치료의 효능을 높일 수 있을 것으로 기대된다.

• Journal of Animal Science and Technology
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• 제61권4호
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• pp.216-224
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• 2019

This study was conducted to investigate the effects of hot melt extrusion (HME) nano-iron as an alternative for the common ferrous sulfate on iron (Fe) bioavailability, growth performance, nutrient digestibility, intestinal morphology, and intestinal microbiota of weanling pigs. A total of 200 piglets (Landrace ${\times}$ Yorkshire ${\times}$ Duroc) were randomly allotted to seven treatments on the basis of initial body weight (BW) and sex. Treatments were the INO100 (100 ppm Fe as $FeSO_4$), HME-Fe levels (50, 75, and 100 ppm nano-Fe as $FeSO_4$). ORG100 (100 ppm Fe as iron methionine). In phase 1, the HME50 pigs showed the lowest Fe content in feed and feces. Plasma Fe concentration was increased in HME100 and ORG100 pigs. In phase 2, there were significantly lower concentration of Fe in feed and feces of HME50 pigs (p < 0.01). A lower Fe concentration in the plasma and liver were observed in HME50 pigs compared with HME100 pigs. Concentration of red blood cell (RBC) was the lowest (p < 0.01) for HME50 pigs. During phase 2, the HME100, HME75, and ORG100 pigs showed a higher RBC and hemoglobin values compared with HME50 pigs. Digestibility of gross energy (GE) and crude protein (CP) were significantly higher in HME100 pigs compared with HME50 pigs. There was an increased (p < 0.01) villus height in the duodenum and jejunum of HME100 pigs compared with HME50 pigs. It is concluded that dietary Fe does not improve growth performance of weanling pigs; however, increasing the dietary iron concentration in weanling piglets increased the RBC and hemoglobin. In addition, the potential ability of HME to be used at a lower level (HME75) was observed.

• 미생물학회지
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• 제55권3호
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• pp.206-212
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• 2019

Propionibacterium acnes (P. acnes)는 모낭과 모공 속에 존재하며 세포 찌꺼기, 피지 및 주변 피부 조직의 대사 부산물을 에너지와 영양소로 사용한다. 과도한 피지생성과 모낭의 막힘으로 피지 생성이 증가하면 P. acnes의 증식 및 성장을 유발할 수 있다. 모낭에 있는 P. acnes의 급속한 성장은 염증을 일으킬 수 있는 세포 손상, 신진 대사 부산물을 생성한다. 본 연구는 S. iscoensis Hieron. (S. iscoensis) 추출물이 P. acnes에 의한 염증반응을 조절할 가능성이 있는지 확인하고, 그 신호 전달 기전을 밝히고자 하였다. S. iscoensis 추출물은 마우스 대식세포주인 Raw 264.7에서 P. acne에 의해 유도되는 IL-$1{\beta}$, TNF-${\alpha}$, iNOS와 같은 염증성 사이토카인의 발현을 매우 효과적으로 억제하였다. 이러한, 염증성 사이토카인의 발현 억제는 NF-${\kappa}B$와 NF-AT와 같은 전사 조절 인자의 활성화 저해를 통해 일어남을 확인하였다. 그러나, MAPK 신호 전달 기전과는 상관이 없음을 확인하였다. 이 연구는 S. iscoensis 추출물이 여드름의 치료제로 사용될 가능성이 있음을 최초로 제안한다.

• Journal of Korean Dental Science
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• 제11권2호
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• pp.71-81
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• 2018

Purpose: The purpose of this study was to investigate the corrosion behaviors of dental implant alloy after microsized surface modification in electrolytes containing Mn ion. Materials and Methods: $Mn-TiO_2$ coatings were prepared on the Ti-6Al-4V alloy for dental implants using a plasma electrolytic oxidation (PEO) method carried out in electrolytes containing different concentrations of Mn, namely, 0%, 5%, and 20%. Potentiodynamic method was employed to examine the corrosion behaviors, and the alternatingcurrent (AC) impedance behaviors were examined in 0.9% NaCl solution at $36.5^{\circ}C{\pm}1.0^{\circ}C$ using a potentiostat and an electrochemical impedance spectroscope. The potentiodynamic test was performed with a scanning rate of $1.667mV\;s^{-1}$ from -1,500 to 2,000 mV. A frequency range of $10^{-1}$ to $10^5Hz$ was used for the electrochemical impedance spectroscopy (EIS) measurements. The amplitude of the AC signal was 10 mV, and 5 points per decade were used. The morphology and structure of the samples were examined using field-emission scanning electron microscopy and thin-film X-ray diffraction. The elemental analysis was performed using energy-dispersive X-ray spectroscopy. Result: The PEO-treated surface exhibited an irregular pore shape, and the pore size and number of the pores increased with an increase in the Mn concentration. For the PEO-treated surface, a higher corrosion current density ($I_{corr}$) and a lower corrosion potential ($E_{corr}$) was obtained as compared to that of the bulk surface. However, the current density in the passive regions ($I_{pass}$) was found to be more stable for the PEO-treated surface than that of the bulk surface. As the Mn concentration increased, the capacitance values of the outer porous layer and the barrier layer decreased, and the polarization resistance of the barrier layers increased. In the case of the Mn/Ca-P coatings, the corroded surface was found to be covered with corrosion products. Conclusion: It is confirmed that corrosion resistance and polarization resistance of PEO-treated alloy increased as Mn content increased, and PEO-treated surface showed lower current density in the passive region.

• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.574-584
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• 2019

Objective: Research was conducted to test the effect of including fiber-rich feedstuffs in practical pig diets on nutrient digestibility, nitrogen balance and ammonia emissions from slurry. Methods: Three Vietnamese fiber sources were screened, namely cassava leaf meal (CL), cassava root residue (CR), and tofu by-product (TF). Accordingly, a control diet (Con) with 10% of dietary non-starch polysaccharides (NSP) and three test diets including one of the three fiber-rich feedstuffs to reach 15% of NSP were formulated. All formulated diets had the same level of crude protein (CP), in vitro ileal protein digestible and metabolisable energy, whereas the in vitro hindgut volatile fatty acid (VFA) production of the test diets was 12% to 20% higher than the control diet. Forty growing barrows with initial body weight at $28.6{\pm}1.93kg$ ($mean{\pm}standard$ deviation) were allocated to the four treatments. When pigs reached about 50 kg of body weight, four pigs from each treatment were used for a nitrogen balance trial and ammonia emission assessment, the remaining six pigs continued the second period of the feeding trial. Results: The TF treatment increased fecal VFA by 33% as compared with the control treatment (p = 0.07), suggesting stimulation of the hindgut fermentation. However, urinary N was not significantly reduced or shifted to fecal N, nor was slurry pH decreased. Accordingly, ammonia emissions were not mitigated. CR and CL treatments failed to enhance in vivo hindgut fermentation, as assessed by fecal VFA and purine bases. On the contrary, the reduction of CP digestibility in the CL treatment enhanced ammonia emissions from slurry. Conclusion: Dietary inclusion of cassava and tofu byproducts through an increase of dietary NSP from 10% to 15% might stimulate fecal VFA excretion but this does not guarantee a reduction in ammonia emissions from slurry, while its interaction with protein digestibility even might enhance enhanced ammonia emission.

• 한국건축시공학회지
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• 제21권5호
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• pp.483-493
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• 2021

도시 인구의 과밀화로 고층 건물에 대한 폭발적인 수요와 공급이 발생하였다. 고층 빌딩은 각 도시의 구심점으로 도시 이미지를 제고하는데 기여하고 있으나 연돌효과로 인해 승강기 문의 오작동, 외벽의 문과 창문 개폐의 어려움, 상층부로 확산하는 연기와 악취, 소음, 에너지 손실, 화재 및 오염물질의 급속한 확산 등 예기치 못한 다양한 문제를 야기하고 있다. 본 연구에서는 초고층 건축물을 수직조닝별로 구분하여 연돌효과의 원인과 해결방안을 분석하고 설계와 시공 방안으로 도출하였다. 외기를 차단하는 초기 계획과 정밀한 시공을 통한 기밀성 확보를 통해 저층부에서 유입되는 기류를 능동적으로 차단하여 건물의 내·외부 기밀성을 확보하는 방안을 모색하였다. 설비적 해결대책은 연돌효과로 인한 특정 현상을 줄이기 위한 대책이 될 수 있으나 이에 따른 2차 피해의 가능성이 높기 때문에 최소한으로 적용해야 한다. 따라서 본 연구는 연돌효과의 원인과 대책방안을 수직 조닝별로 설계적, 시공적 대책을 제시함으로써 체계적인 연돌효과 관리의 필요성을 역설하였다. 또한 본 연구가 설계 및 시공뿐만 아니라 건물 유지관리에도 도움이 되기를 기대한다.

• Structural Engineering and Mechanics
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• 제76권6호
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• pp.687-708
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• 2020

Openings in steel plate shear walls (SPSWs) are usually used for decorative designs, crossing locations of multiple utilities and/or structural objectives. However, earlier studies showed that generating an opening in an SPSW has a negative effect on the cyclic performance of the SPSW. Therefore, this study proposes tripling or doubling the steel-sheet-plate (SSP) layer and stiffening the opening of the SPSW to provide a solution to undesirable opening effects, improve the SPSW performance and provide the infill option of potential strengthening measures after the construction stage. The study aims to investigate the impact of SSP doubling with a stiffened opening on the cyclic behaviour, expand the essential data required by structural designers and quantify the SPSW performance factors. Validated numerical models were adopted to identify the influence of the chosen parameters on the cyclic capacity, energy dissipation, ductility, seismic performance factors (SPF) and stiffness of the suggested method. A finite Element (FE) analysis was performed via Abaqus/CAE software on half-scale single-story models of SPSWs exposed to cyclic loading. The key parameters included the number of SSP layers, the opening size ratios corresponding to the net width of the SSP, and the opening shape. The findings showed that the proposed assembly method found a negligible influence in the shear capacity with opening sizes of 10, 15, 20%. However, a deterioration in the wall strength was observed for openings with sizes of 25% and 30%. The circular opening is preferable compared with the square opening. Moreover, for all the models, the average value of the obtained ductility did not show substantial changes and the ultimate shear resistance was achieved after reaching a drift ratio of 4.36%. Additionally, the equivalent sectional area of the SSP in the twin and triple configuration of the SPSWs demonstrated approximately similar results. Compared with the single SSP layer, the proposed configuration of the twin SSP layer with a stiffened opening suggest to more sufficiency create SSP openings in the SPSW compared to that of other configurations. Finally, a tabular SPF quantification is exhibited for SPSWs with openings.