• Journal of Life Science
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• v.26 no.12
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• pp.1446-1457
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• 2016

Wetlands exhibit intermediate characteristics of both terrestrial and aquatic ecosystems, and the biodiversity is rich in these unique biological habitats. The symbiotic relationships between plants and fungi that inhabit these wetlands play an important role in natural resource management, biodiversity, and conservation. Accordingly, the mujechi, having academic value for the study of the natural environment, was investigated in terms of genetic diversity of endophytic fungi, which inhabit the roots of wild plants. The internal transcribed spacer (ITS) region was amplified to identify fungal strains. In total, 226 strains were isolated and categorized into three phyla, seven classes, 10 orders, 22 families, and 31 genera. In plants by endophytic fungi were classified in Isachne globosa (Ig) to 19 genera, Scirpus karuisawensis (Sk) to 11 genera, Utricularia racemosa (Ur) to 19 genera, and one incertae sedis, Eriocaulon decemflorum (Ed) to 11 genera. The fungal taxa was identified the genera Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), and Perenniporia (7.1%). The fungal group isolated from Isachne globosa (Ig) grew the largest number of isolated fungal strains. After comprehensive evaluation, the endophytic fungal group from Utricularia racemosa (Ur) ranked highest in diversity analyses. From the roots of wild plant in mujechi-neup, it confirmed the distribution and diversity of endophytic fungi. This study provides the basic data to understand fungal community structure in peat wetlands.

• Journal of Life Science
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• v.21 no.11
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• pp.1619-1624
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• 2011

Endophytic fungal strains were isolated from the roots of six species plants in the Dokdo islands. Native plant samples, such as Artemisia japonica, Chenopodium album and Solanum nigrum were isolated from Dongdo, and those such as Cyrtomium falcatum, Dianthus longicalyx and Tetragonia tetragonoides were isolated from Seodo. In total, thirty two fungal strains were isolated from these native plants. To identify the fungal strains, polymerase chain reaction (PCR) amplification of internal transcribed spacer (ITS: containing ITS1, 5.8s and ITS2 region) regions was done with universal primers ITS1 and ITS4. Endophytic fungi of four species were isolated from A. japonica, eight species from C. album, three species from S. nigrum, three species from C. falcatum, three species from D. longicalyx and eleven species from T. tetragonoides. Culture filtrates (CF) of isolated endophytic fungi were used to treatwaito-c rice seedlings to test plant growth-promoting activity. As a result of bioassay, Ca-5-2-2 strain isolated from C. album expressed highest plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp., Fusarium sp. and Aspergillus sp. were the most abundantly distributed fungal strains in the six plants used in this study.

• Journal of Life Science
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• v.23 no.1
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• pp.95-101
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• 2013

In this study, plant samples of five species were collected from the Dokdo islands in South Korea. Plant samples such as Asparagus schoberioides, Corydalis platycarpa, Festuca rubra, Sedum oryzifolium, and Setaria viridis were collected from the Dongdo and Seodo. Endophytic fungal strains were isolated from the roots of five plants from the Dokdo islands. Thirty-three fungal strains were isolated from these native plants. All the endophytic fungi were analyzed by internal transcribed spacer (ITS) sequencing (ITS containing ITS1, 5.8s, and the ITS2 region). Waito-c rice seedlings were treated with fungal culture filtrates to test their plant growth-promoting activity. A bioassay of the D-So-1-1 fungal strain isolated from S. oryzifolium confirmed that it has the highest plant growth-promoting activity. All the endophytic fungi belong to four orders: Eurotiales (86%), Capnodiales (3%), Hypocreales (4%), and Incertae sedis (7%). The endophytic fungi were classified as Ascomycota, which contained Aspergillus (12%), Cladosporium (3%), Eurotium (3%), Fusarium (18%), Microsphaeropsis (6%), and Penicillium (58%) at the genus level.

• Journal of Life Science
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• v.22 no.10
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• pp.1384-1391
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• 2012

Five coastal plant species, Artemisia fukudo, Aster sphathulifolius, Plantago camtschatica, Sedum oryzifolium, and Setaria viridis, were collected from the coastal region of Ulleung Island (Ulleung-Do, South Korea). Thirty-six endophytic fungi were isolated from the roots of these plants, and all were identified by using PCR with the following specifications: internal transcribed spacer 1 (ITS1), 5.8S rRNA, and ITS2 regions. Phylogenetic analysis indicated that all fungal strains belong to the phylum Ascomycota and comprise four orders (Capnodiales, Eurotiales, Hypocreales, and Pleosporales). Among all the identified species, the Eurotiales species were more abundant than species in the other orders. Nine different genera (Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma, and Pyrenochaeta) in the four orders were confirmed. Penicillium and Aspergillus species were the most dominant species among the endophytic fungi isolated from the coastal plants. Shannon's diversity index (H') ranged from 0.684 to 1.609, and the endophytic fungi in S. oryzifolium was more diverse compared to the endophytic fungi in the other plants.

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.21.1-21.1
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• 2008

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.100.2-100.2
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• 2008

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.100.1-100.1
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• 2008

• Journal of Microbiology and Biotechnology
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• v.17 no.8
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• pp.1361-1368
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• 2007

Endophytic bacteria associated with the roots of coastal sand dune plants were isolated, taxonomically characterized, and tested for their plant growth-promoting activities. Ninety-one endophytic bacterial isolates were collected and assigned to 17 different genera of 6 major bacterial phyla based on partial 16S rDNA sequence analyses. Gammaproteobacteria represented the majority of the isolates (65.9%), and members of Pseudomonas constituted 49.5% of the total isolates. When testing for antagonism towards plant pathogenic fungi, 25 strains were antagonistic towards Rhizoctonia solani, 57 strains were antagonistic towards Pythium ultimum, 53 strains were antagonistic towards Fusarium oxysporum, and 41 strains were antagonistic towards Botrytis cinerea. Seven strains were shown to produce indole acetic acid (IAA), 33 to produce siderophores, 23 to produce protease, 37 to produce pectinase, and 38 to produce chitinase. The broadest spectra of activities were observed among the Pseudomonas strains, indicating outstanding plant growth-promoting potential. The isolates from C. kobomugi and M. sibirica also exhibited good plant growth-promoting potential. The correlations among individual plant growth-promoting activities were examined using phi coefficients, and the resulting data indicated that the production of protease, pectinase, chitinase, and siderophores was highly related.

• The Korean Journal of Mycology
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• v.43 no.1
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• pp.13-19
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• 2015

Endophytic fungal strains were isolated from 5 aquatic plant species (Trapa japonica, Miscanthus sacchariflorus, Oenanthe javanica, Typha orientalis and Zizania latifolia) native to river wetland of tributary in Nakdong river. Total 34 strains were purely isolated, and then its internal transcribed spacer (ITS) regions were amplified. After that, phylogenetic analysis based on ITS sequences and deduction of diversity indices were done. Fungal isolates were belonged to 17 genera, concretely in Acremonium, Alternaria, Aspergillus, Cladosporium, Emericellopsis, Fusarium, Galactomyces, Leptosphaeria, Microsphaeropsis, Penicillium, Peyronellaea, Phoma, Pseudeurotium, Rhizomucor, Talaromyces, Trematosphaeria and Zalerion. Especially, fungal isolates were distributed intensively in genera Alternaria and Talaromyces. This study deals with the diversity of endophytic fungal species that showing symbiotic relationship with their host aquatic plants.

• Mycobiology
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• v.46 no.2
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• pp.85-91
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• 2018

Endophytic fungi strains (n = 81) were isolated from the leaves, barks, and fruits of Camellia oleifera from Hunan province (China) to delineate their species composition and potential as biological control agents of C. oleifera anthracnose. The fungi were identified by morphological and phylogenetic analyses. Fungal colonization rates of the leaves, barks, and fruits were 58.02, 27.16, and 14.81%, respectively. The isolates were identified as 14 genera, belonging to two subdivisions, Deuteromycotina and Ascomycotina; 87.65% of all isolates belonged to Deuteromycotina. The dominant species, occurring with a high relative frequency, were Pestalotiopsis sp. (14.81%), Penicillium sp. (14.81%), and Fusarium sp. (12.35%). The Simpson's and Shannon's diversity indices revealed the highest species diversity in the leaves, followed by the barks and fruits. The similarity index for the leaves versus barks comparison was the highest, indicating that the number of endophytic fungal species shared by the leaves and barks was higher than barks and fruits or leaves and fruits. Based on the results of dual culture experiments, only five strains exhibited antifungal activity against C. oleifera anthracnose pathogen, with isolate ty-64 (Oidium sp.) generating the broadest inhibition zones. Our results indicate that the endophytes associated with C. oleifera could be employed as natural agents controlling C. oleifera anthracnose.