Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Plant Growth-Promoting Activity and Genetic Diversity of Endophytic Fungi Isolated from Native Plants in Dokdo Islands for Restoration of a Coastal Ecosystem

해안 생태계의 복원을 위하여 독도에 자생하는 식물로부터 분리된 내생진균류의 식물생장촉진활성과 유전학적 다양성

  • You, Young-Hyun (School of Life Science, Kyungpook National University) ;
  • Yoon, Hyeokjun (School of Life Science, Kyungpook National University) ;
  • Kim, Hyun (School of Life Science, Kyungpook National University) ;
  • Lim, Sung Hwan (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • Received : 2012.10.22
  • Accepted : 2013.01.03
  • Published : 2013.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, plant samples of five species were collected from the Dokdo islands in South Korea. Plant samples such as Asparagus schoberioides, Corydalis platycarpa, Festuca rubra, Sedum oryzifolium, and Setaria viridis were collected from the Dongdo and Seodo. Endophytic fungal strains were isolated from the roots of five plants from the Dokdo islands. Thirty-three fungal strains were isolated from these native plants. All the endophytic fungi were analyzed by internal transcribed spacer (ITS) sequencing (ITS containing ITS1, 5.8s, and the ITS2 region). Waito-c rice seedlings were treated with fungal culture filtrates to test their plant growth-promoting activity. A bioassay of the D-So-1-1 fungal strain isolated from S. oryzifolium confirmed that it has the highest plant growth-promoting activity. All the endophytic fungi belong to four orders: Eurotiales (86%), Capnodiales (3%), Hypocreales (4%), and Incertae sedis (7%). The endophytic fungi were classified as Ascomycota, which contained Aspergillus (12%), Cladosporium (3%), Eurotium (3%), Fusarium (18%), Microsphaeropsis (6%), and Penicillium (58%) at the genus level.

본 연구에서는 독도에 자생하는 5종의 식물, 비짜루, 갯괴불주머니, 왕김의 털, 땅채송화, 갯강아지풀을 채집하였다. 독도의 자생식물 뿌리로부터 33 주의 내생진균을 분리하였다. 분리된 모든 내생진균류들은 ITS1, 5.8s와 ITS2를 포함하는 ITS영역의 서열에 의해 분석되었다. 그리고 내생진균류의 배양여과액을 이용하여 난장이벼 유묘에 처리하여 식물생장촉진활성을 확인하였다. 그 결과, 땅채송화로부터 분리된 D-So-1-1 내생진균이 가장 우수한 식물생장촉진활성을 나타내었다. 그리고 분리된 모든 내생진균는 자낭균문의 Eurotiales (86%), Capnodiales (3%), Hypocreales (4%), 및 Incertae sedis (7%)에 속하는 것을 확인하였다. 그리고 내생진균류를 속으로 분류하였을 때, Aspergillus (12%), Cladosporium (3%), Eurotium (3%), Fusarium (18%), Microsphaeropsis (6%), 및 Penicillium (58%)인 것으로 나타났다.

Keywords

References

  1. Clay, K. and Holah, J. 1999. Fungal endophyte symbiosis and plant diversity in successional fields. Science 285, 1742-1744. https://doi.org/10.1126/science.285.5434.1742
  2. Ham, M. S., Park, Y. M., Sung, H. R., Sumayo, M., Ryu, C. M., Park, S. H. and Ghim, S. Y. 2009. Characterization of rhizobacteria isolated from family Solanaceae plants in Dokdo island. Kor J Microbiol Biotechnol 37, 110-117.
  3. Hasan, H. A. 2002. Gibberellin and auxin-production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol Immunol Hung 49, 105-118. https://doi.org/10.1556/AMicr.49.2002.1.11
  4. Hwang, J. S., You, H. Y., Bae, J. J., Khan, S. A., Kim, J. G. and Choo, Y. S. 2011. Effects of endophytic fungal secondary metabolites on the growth and physiological response of Carex kobomugi Ohwi. J Coastal Res 27, 544-548. https://doi.org/10.2112/JCOASTRES-D-10-00090.1
  5. Jeon, Y. G. 2005. The regional geomorphology of Dokdo (Volcanic Island). Kor J Reg Geol 11, 19-28.
  6. Kim, M. H., Oh, Y. J., Kim, C. S., Han, M. S., Lee, J. T. and Na, Y. E. 2007. The Flora and Vegetation Distribution in Dokdo. Kor J Environ Agric 26, 85-93. https://doi.org/10.5338/KJEA.2007.26.1.085
  7. Khan, S. A., Hamayun, M., Rim, S. O., Lee, I. J., Seu, J. C., Choo, Y. S., Lee, I. J., J, Kim, S. D., Lee, I. K. and Kim, J. G. 2008. Isolation of endophytic fungi capable of plant growth promotion from monocots inhibited in the coastal sand dunes of Korea. J Life Sci 18, 1355-1359. https://doi.org/10.5352/JLS.2008.18.10.1355
  8. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Lee, I. J. and Kim, J. G. 2009. Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum. World J Microbiol Biotechnol 25, 829-833. https://doi.org/10.1007/s11274-009-9981-x
  9. Khan, S. A., Hamayun, M., Yoon, H. J., Kim, H. Y., Suh, S. J., Hwang, S. K., Kim, J. M., Lee, I. J., Choo, Y. S., Yoon, U. H., Kong, W. S., Lee, B. M. and Kim, J. G. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiol 8, 231. https://doi.org/10.1186/1471-2180-8-231
  10. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Seo, J. C., Choo, Y. S., Kil, Y. J., Eo, J. K. and Eom, A. H. 2009. Molecular identification and diveristy of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor J Mycol 37, 130-133. https://doi.org/10.4489/KJM.2009.37.2.130
  11. Khan, S. A., Hamayun, M., Kim, H. Y., Yoon, H. J., Seo, J. C., Choo, Y. S., Lee, I. J., Kim, S. D., Rhee, I. K. and Kim, J. G. 2009. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production. Biotechnol Lett 31, 283-287. https://doi.org/10.1007/s10529-008-9862-7
  12. Lee, D. B. and Joo, S. U. 1958. Reinvestigation of the flora of the Dagelet Island. Humani Sci Nat Sci 3, 223-296.
  13. Lee, D. H., Cho, S. H. and Pak, J. H. 2007. The analysis of vascular plant species composition in Dok-do island. Kor J Plant Taxon 37, 545- 563.
  14. Park, S. J., Song, I. G., Park, S. J. and Lim, D. O. 2010. The Flora and Vegetation of Dokdo Island in Ulleung-gun, Gyeongsanbuk-do. Kor J Env Eco 24, 264-278.
  15. Research Institute for Ullengdo & Dokdo Islands. 2008. pp.166-221, The plant of Dokdo island. Nature of Dokdo island. Kyungpook National University Press. Daegu. Korea.
  16. Seo, S. T., Kim, K. H., Kim, M. J., Hong, J. S., Park, J. H. and Shin, S. C. 2009. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Kor J Mycol 37, 108-110. https://doi.org/10.4489/KJM.2009.37.1.108
  17. Sun, B. Y., Sul, M. R., Im, J. A. Kim, C. H. and Kim, T. J. 2002. Evolution of endemic vascular plants of Ulleungdo and Dokdo in Korea - floristic and cytotaxonomic characteristics of vascular flora of Dokdo. Kor J Plant Taxon 32, 143-158.
  18. Shin, H. T., Park, S. J., Kang, K. H. and Yoo, J. H. 2004. The establishment of conservation area and conservation strategy in Ulnung Island. Kor J Env Eco 18, 221-230.
  19. Vazquez, M., Cesar, M., Azcon, S. R. and Barea, J. M. 2000. Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants. Appl Soil Ecol 15, 261-272. https://doi.org/10.1016/S0929-1393(00)00075-5
  20. Yamada, A., Takeo, O., Yosuke, D. and Masatake, O. 2001. Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas. Mycoscience 42, 43-50. https://doi.org/10.1007/BF02463974
  21. Yoon, J. H., Kang, S. Y., Lee, M. H. and Oh, T. K. 2005. Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korean. Int J Syst Evol Microbiol 51, 1079-1086.
  22. You, Y. H., Yoon, H., Lee, G. S., Woo, J. R., Shin, J. H., Lee, I. J., Rim, S. O., Choo, Y. S. and Kim, J. G. 2011. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. J Life Sci 21, 992-996. https://doi.org/10.5352/JLS.2011.21.7.992
  23. You, Y. H., Yoon, H., Woo, J. R., Seo, Y., Kim, M., Choo, Y. S. and Kim, J. G. 2011. Plant growth-promoting activity of endophytic fungi isolated from the roots of native plants in Dokdo islands. J Life Sci 21, 1619-1624. https://doi.org/10.5352/JLS.2011.21.11.1619
  24. You, Y. H, Yoon, H. Kang, S. M., Shin, J. H., Choo, Y. S., Lee, I. J., Lee, J. M. and Kim, J. G. 2012. Fungal diversity and plant growth promotion of endophytic fungi from six halophytes in Suncheon Bay. J Microbiol Biotechnol 22, 1550-1557.
  25. You, Y. H., Yoon, H., Seo, Y., Kim, M., Kang, M. S., Kim, C., Ha, S. C., Cho, G. Y. and Kim, J. G. 2012. Genetic diversity of culturable endophytic fungi Isolated from halophytes naturally growing in Muan salt marsh. J Life Sci 22, 970-980. https://doi.org/10.5352/JLS.2012.22.7.970

Cited by

  1. Secondary metabolites from the endophytic fungus Talaromyces pinophilus vol.31, pp.15, 2017, https://doi.org/10.1080/14786419.2017.1290624
  2. Confirmation of Two Undescribed Fungal Species from Dokdo of Korea Based on Current Classification System Using Multi Loci vol.43, pp.4, 2015, https://doi.org/10.5941/MYCO.2015.43.4.392
  3. Metagenomic analysis of soil fungal communities on Ulleungdo and Dokdo Islands vol.61, pp.3, 2015, https://doi.org/10.2323/jgam.61.67
  4. Gibberellin A7 production by Aspergillus tubingensis YH103 and cultural characteristics of endophytic fungi isolated from Tetragonia tetragonoides in Dokdo islands vol.52, pp.1, 2016, https://doi.org/10.7845/kjm.2016.5071
  5. A strategy for securing unique microbial resources – focusing on Dokdo islands-derived microbial resources pp.2224-4662, 2018, https://doi.org/10.1163/22244662-20181024
  6. Four New Records of Ascomycete Species from Korea vol.46, pp.4, 2018, https://doi.org/10.1080/12298093.2018.1550169