Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Coffee-like Green Tea Preparation on Cytotoxicity of Human Cancer and Normal Cells

Coffee-like green tea의 인체암세포 및 정상세포에 대한 독성

  • Moon, Yean Guy (Division of Applied Life Science (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kwon, Jung Min (Division of Applied Life Science (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim, Jong Cheol (Institute of Hadong Green Tea) ;
  • Park, Han Min (Department of Environmental Materials Science, Gyeongsang National University) ;
  • Cho, Yong Un (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Jung, Kwan Ju (Bio21 center) ;
  • Ha, Yeong Lea (Division of Applied Life Science (BK21 Program), Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University)
  • 문연규 (경상대학교 대학원 응용생명과학부) ;
  • 권애경 (경상대학교 대학원 응용생명과학부) ;
  • 김종철 ((재)하동녹차연구소) ;
  • 박한민 (경상대학교 환경재료과학과) ;
  • 조용운 (경남과학기술대학 제약공학과) ;
  • 정관주 ((재)바이오21센터) ;
  • 하영래 (경상대학교 대학원 응용생명과학부)
  • Received : 2012.10.07
  • Accepted : 2013.01.10
  • Published : 2013.01.30
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Abstract

The cytotoxicity of coffee-like green tea (CLGT) was determined in a human breast cancer cell line, MCF-7; a human prostate cancer cell clone, PC-3; a human neuroblastoma cell line, SK-N-SH; and a rat cardiomyoblast cell line, H9c2, with reference to green tea leaves (GTL). The CLGT was prepared by roasting the GTL for 60 min at $240^{\circ}C$ in a temperature-controlled frying pan. The CLGT preparation imitated the flavor and taste characteristics of coffee fairly well according to sensory analysis. The CLGT preparation had no adverse cytotoxic effects on the cancer cells or the normal cells compared to GTL. No significant change in the antioxidant activity was seen in the CLGT preparation compared to that of GTL. The amount of total protein, sugar, and phenolic compounds was reduced in the preparation relative to those in GTL, a fact that might explain the coffee-like flavor and/or taste characteristics of the CLGT preparation. These results suggest that CLGT prepared by roasting GTL for 60 min at $240^{\circ}C$ does not show any adverse effects on cancer cells and normal cells compared to GTL. They imply that CLGT could be safe for human consumption.

커피와 유사한 향미기를 갖는 녹차(Coffee-like green tea: CLGT)를 제조하고 이의 인체 유방암세포 주 MCF-7, 인체 전립선암세포 주 PC-3, 인체 신경모세포 주 SK-N-SH 및 쥐 심근세포 주 H9c2에 대한 세포독성을 연구하였다. 녹차엽(GTL)을 $240^{\circ}C$에서 1시간 roasting한 녹차가 커피와 가장 유사한 향미를 나타내었다. CLGT는 GTL과 비교하여 인체 암세포 및 정상세포와 쥐 심근세포에 대한 세포독성 차이가 없었다. GTL의 roasting은 ECG 등의 catechin 성분과 total protein은 유의성 있게 감소시켰지만, total phenol 및 total sugar는 유의성 있는 감소시키지 않아, 이와 같은 화합물 조성의 변화가 GTL에 커피유사 향미를 제공하였을 것이다. 따라서 이들 결과는 CLGT이 인체에 안전하여 커피 대용품으로 사용할 수 있음을 의미한다.

Keywords

References

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