• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.517-527
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• 1994

An attempt was made to isolate a causative viral agents from the fecal specimens of the diseased dogs with the gastroenteritis symptoms. Two coronavirus-like agents were isolated by serial dilution end point method and plaque assay. The isolates were characterized in terms of cytopathology, antigenicity, replication, physicochemical and morphological properties. The results obtained through the experiment were as follows; 1. Among 7 fecal specimens collected from the dogs with enteric disease, 2(28.6%)coronavirus-like agents showing typical cytopathic effects of canine coronavirus were isolated, and designated as CCV D1 and CCV D2, respectively. 2. By the cross-neutralization test and indirect immunofluoresence antibody test, the isolates were antigenically indentified as the standard CCV. The viruses were replicated only in the cytoplasm of A-72 cells. 3. The isolates showed no haemagglutinating activity against the erythrocytes from 11 kinds of animals. 4. The electron microscopic observation for the isolates showed spherical and pleomorphic features, covered with club-shaped projections on the surface. The size of particles was ranged from 70 to 150nm. 5. In one-step growth curve for the isolates in A-72 cells, maximum titers of intracellular vius was $10^{4.6}$ $TCID_{50}/0.1ml$ at 46 hrs postinoculation(pi) of CCV Dl and $10^{4.4}$ $TCID_{50}/0.1ml$ at 34 hrs pi of CCV D2. The maximum titers of extracellular virus was $10^{5.5}$ $TCID_{50}/0.1ml$ at 58 hrs pi of CCV D1 and $10^{5.8}$ $TCID_{50}/0.1ml$ at 46 hrs pi of CCV D2. 6. In physicochemical property test, the isolates were very sensitive to choroform and were found to be RNA virus. The viruses was stable at pH 3.0 for 1 hr and at $22{^{\circ}C}$ for 5 hrs. However, infectivity titers reduced remarkably by treatment with $56{^{\circ}C}$ for 10min.

• Journal of Korea Water Resources Association
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• v.45 no.10
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• pp.1069-1079
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• 2012

In this study, to analyze travelling route and transition characteristics which is a spatial time interpretation method now actively progressed in domestic as well as abroad, it was intended to develop new drought interpretation technique which can decide the centroid and orbit of drought through assuming ellipse using Mass Moment concept. First of all, after estimating Standard Precipitation Index (SPI) per different precipitation observatory station to extract drought events, by selecting precipitation sites where drought starting and end point are same, these were categorized as CASE. As a results, with various CASE selections falling in specific duration for monthly drought analysis, it is possible to find out drought area that additionally occurred, and drought reliving process could be confirmed more definitely. Therefore, if the research methods adopted in this study for drought monitoring are utilized, not only accurate spatio-temporal drought analysis is possible, also pattern of drought centroid movement can be analyzed by establishing statistically significant spatial characteristics data after separating all the drought events that occurred sporadically in Korea Peninsula.

• Journal of Food Hygiene and Safety
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• v.15 no.3
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• pp.186-198
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• 2000

The quality of the management of sanitation in food service establishments (school lunch programs, hospital patient food services, and commercial catering food services) in Korea was reviewed and evaluated, and ten strategies fur future improvement were suggested. They were: (1) An increase of qualified manpower and improvement of the professional training of the staff; (2) Obtaining special facilities exclusively for food service; (3) Improvement of facilities especially the kitchens; (4) Improvement of policy fur procuring raw materials and being assured of their quality by designing some standards and specifications for the raw materials to be purchased; (5) Production and use accurate and reliable kitchen apparatus and instruments; (6) An increase of the laboratory apparatus and instruments for inspection and evaluation of the sanitary level of raw materials and food service environments; (7) Enforced improvement of personal hygiene of the staff; (8) Use of a variety of methods in sanitary education and training; (9) Actively inspect the quality of imported foods; (10) Strengthening the research and accumulation of background data regarding sanitation management. There is a long process from the production of food to eating. The cooking process is the ultimate end of preparation of food before eating. This process sometimes increases the occurrence of food-borne diseases if we mishandle the food, even we obtained safe food. The process can also remove health hazards and reduce the risk from the hazards if we handle the food well although we have unsafe foods. This means the cooking process is a major key to preventing food-borne diseases. The concepts of hazard analysis critical control point (HACCP) should be applied and practiced in food service establishments in Korea as soon as possible.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1182-1182
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• 2001

In this study, the newly constructed optical measurement system, which was mainly composed of a parametric tunable laser and a near infrared photoelectric multiplier, was introduced to clarify the optical characteristics of wood as discontinuous body with anisotropic cellular structure from the viewpoint of the time-of-flight near infrared spectroscopy (TOF-NIRS). The combined effects of the cellular structure of wood sample, the wavelength of the laser beam λ, and the detection position of transmitted light on the time resolved profiles were investigated in detail. The variation of the attenuance of peak maxima At, the time delay of peak maxima Δt and the variation of full width at half maximum Δw were strongly dependent on the feature of cellular structure of a sample and the wavelength of the laser beam. The substantial optical path length became about 30 to 35 times as long as sample thickness except the absorption band of water. Δt ${\times}$ Δw representing the light scattering condition increased exponentially with the sample thickness or the distance between the irradiation point and the end of sample. Around the λ=900-950 nm, there may be considerable light scattering in the lumen of tracheid, which is multiple specular reflection and easy to propagate along the length of wood fiber. Such tendency was remarkable for soft wood with the aggregate of thin layers of cell walls. When we apply TOF-NIRS to the cellular structural materials like wood, it is very important to give attention to the difference in the light scattering within cell wall and the multiple specular-like reflections between cell walls. We tried to express the characteristics of the time resolved profile on the basis of the optical parameters for light propagation determined by the previous studies, which were absorption coefficient K and scattering coefficient S from Kubelka-Munk theory and n from nth power cosine model of radiant intensity. The wavelength dependency of the product of K/S and n, which expressed the light-absorbing and -scattering condition and the degree of anisotropy, respectively, was similar to that of the time delay of peak maxima Δt. The variation of the time resolved profile is governed by the combination of these parameters. So, we can easily find the set of parameters for light propagation synthetically from Δt.

• 한국정보교육학회:학술대회논문집
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• 2010.08a
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• pp.15-18
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• 2010

As robots are proved to be effective in enhancing students' creativity and problem-solving abilities and satisfying various needs in special education for the gifted, many students participate in private education and after-school robot classes. However, it is difficult for students in the lower social economy class to use robots for their learning because of the high expense of robots. On this point, as a part of u-Learnng project, this research attempts to provide students in the lower social economy class with the opportunities to use robots for one year. At the end of the year, we will compare the experimental group and the control group in order to examine learning effects of using robots. Until now we have found many cases that show positive effects of the use of robots in students' learning.

• The Korean Journal of Zoology
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• v.30 no.1
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• pp.1-9
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• 1987

Cyclic AMP (cAMP) was known to play a key role in the regulation of cumulus expansion and oocyte maturation of mammalian cumulus-oocyte complexes (COC's) in vivo and in vitro. The present experiments were conducted to know how intracellular level of cAMP in these cells is controlled. Intracellular cAMP level was modulated by culturing mouse CGC's with an adenylate cyclase stimulator, forskolin, a phosphodiesterase inhibitor, 3-isobutyl-1-methyixanthine (IBMX), human chorionic gonadotrophin (HCG), or follicle stimulating hormone (FSH). The rate of cumulus expansion and germinal vesicle break-down (GVBD) was checked after culture and used as a biological end point. Forskolin in the medium began to stimulate the expansion of the complexes at 1 nM and induced maximum expansion (80~90%) at 0 1~10 $\mu$M. The expansion rate was reduced to 60% when forskolin concentration was increased to 100 $\mu$M. Oocyte GVBD occurred normally (75~82%) in the presence of 10 $\mu$M of forskolin, but partial suppression was appeared at 100 pM of the drug (40%). IBMX also stimulated the expansion from the concentration of 0.01 pM and induced full expansion (81~89%) between the concentration of 1-1000 $\mu$M. Meiotic resumption was occurred normally under 10 $\mu$M of IBMX, but suppressed drastically from the concentration of 100 $\mu$M. The minimum exposing time to hormone or drugs required to trigger cumulus expansion was two minutes with HCG, 15~30 minutes with FSH and fors kolin, and two hours with IBMX. The data presented here seemed to imply that intracellular cAMP level in cumulus cells is regulated by both adenylate cyclase and phosphodiesterase and cumulus expansion is induced by a peak of cAMP while meiotic arrest is maintained by continuous presence of cAMP.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.562-573
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• 1997

Comparative osteology of Chromis notata, C. analis and C. fumea was studied based on the samples collected from the Korean waters. The three species are distinguished by the shape of cranium, visceral skeleton, vertebra and caudal skeleton. Chromis notata has a glossohyal which is slightly curved in shape while two other species are deeply curved. C. analis has three spiniform procurrent caudal rays while others have two. C. fumes could be distinguished easily by the shape of preopercle which is serrate in posterior margin. This investigator reveals following bones as new taxonomic criteria for the genus Chromis in Korea. In cranium, height of supraoccipital, height of process of parasphenoid and anterior margin of basisphenoid, in visceral skeleton, presence of fine papilla-like process at the surface of preopercular sensory canal, in orbital bone, width of preorbital, in hyoid arch, shape of glossohyal, union state between lower hypohyal and ceratohyal and union state between ceratohyal and epihyal, in shoulder girdle bone, dorsal margin of posttemporal, width of upper postclavicle, posterior end of lower postclavicle and posterior margin of first actinost bone, in vertebrae, beginning point of parapophysis, in caudal skeleton, upper shape of second neural spine of pleural centrum.

• Journal of Nutrition and Health
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• v.34 no.1
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• pp.14-22
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• 2001

In this study, we examined the effects of dietary fatty acids and vitamin E supplementation on antioxidant systems in the rat brain regions. The Sprague Dawley rats were fed the experimental diets 3-4 wks prior to the conception. Experimental diet consisted of 10% fat(wt/wt) which were safflower oil(SO, poor in $\omega$3 fatty acids), mixed oil(MO, P/M/S ratio=1.03:1.45:1,$\omega$6/$\omega$3 ratio=6.3) and mixed oil supplemented with vitamin E(ME:MO+500mg vitamin E/kg diet). At 3 and 9 weeks of age of the newborn rats, frontal cortex(FC), corpus striatum(CS), hippocampus(H) cerebellum(CB) were dissected out from the whole brain. Activities of glutathione peroxidase(GSH-P(sub)x, superoxide dismutase(SOD) concentrations of malondialdehyde(MDA) were mesaured. Dietary fatty acids were not effective in antioxidative system for rat brain. However, when vitamin E was supplemented to the diet(ME), the activities of GSH-P(suh)x tended to increase in comparison to MO group. Therefore, the activites of GSH-P(suh)x of FC and H at the age of 3 weeks showed significant differences(p<0.05). The activities of Total-SOD tended to decrease in ME group compared to MO group. There were significant differences(p<0.05) in FC and CS at the age of 3 weeks. The activities of Mn-SOD tended to increase and Cu, Zn-SOD tended to decrease when vitamin E was supplemented. The activity levels of antioxidative enzymes at the age of 3 weeks and 9 weeks were similar. This suggested that the activity level of antioxidative enzymes reached to the adult level at the age of 3 weeks which is the end point of lactation period. The concentrations of MDA were not altered by experimental diets. When the activities of antioxidant enzymes were compared, the activities of antioxidant enzymes were the lowest in H and FC. In conclusion, the antioxidative system were not altered by dietary fatty acid at the age of 3 weeks and 9 weeks, but the supplementation of vitamin E altered the antioxidative systems. Therefore, these findings should be considered comprehensively in scope of the balance of various antioxidative systems and their interactions(Korean J Nutrition 34(1):14-22, 2001)

• Journal of Korean Library and Information Science Society
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• v.46 no.3
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• pp.349-379
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• 2015

In the 1980's opening 79 of the end of 2013, the National Public Library that had been It was 885 its opening remains in effect. Especially in the 2000s after 60% of the whole library, a library erected since the advent of the library is enough to be a significant quantitative expansion. The resulting to make a qualitative aspect of public libraries and increase users' needs for efficient space configuration. IIn particular, the user is to serve the needs of digital reading room and the increase of the cultural training changes after 2000 years ago, and appears as due to the implications of space configuration in an age-appropriate library space off the rate plan is a necessary point. The study in the 1980s-1990s, built public library established since the year 2000 centered on public library mutual comparative analysis of space configuration form. Book reading function was the conservation and highlight 1980-1990s and considered by the side of the users culture area extended since the 2000s, public library takes the difference between the ratio of the area of the facility by the area in which you can meet the standards required and the age.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.12
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• pp.2712-2716
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• 2011

The development of mobile phone is growing everyday according to the change of our age. Most of all, the birth of smart phone has gotten lots of attentions. The Smart Phone has not only nalogue functions for calling and receiving but also has many multiple-service functions satisfying the contemporary digital needs. Due to the smart phone, people could access to various and plenty information simply by touching it. It was Sep. 2011 that the number of smart phone users began to be more than 15,000,000. That number stands for the turning point of digital revolution. In Korea, both of "Omnia" from Samsung and "i-phone" from Apple came into the market near the end of 2009. That time was so called the full-scale first smart-phone war, and thinking of the time, it could be named as the explosive growth of smart phones. We can predict that the smart phone will influence the real life of human beings not only the mobile phone market. In this way, the smart phone has come deeply into our life day by day. For instance, more people can be able to get the informations of their trip for their leisure and free weekends through the smart phone. Thus, our study, began with the theme of "Korean islands trip", will start in the relationship with the Application-Services, "Islands" utilizing web-pictures.