Korean Journal of Veterinary Research (대한수의학회지)
- Volume 34 Issue 3
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- Pages.517-527
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- 1994
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Characterization of canine coronaviruses isolated from the dogs with diarrhea in Korea
설사증 이환견(犬)으로 부터 분리(分離)한 canine coronavirus의 성상(性狀)에 관한 연구(硏究)
- Lee, Byung-hyung (College of Veterinary Medicine, Chungnam National University) ;
- Jun, Moo-hyung (College of Veterinary Medicine, Chungnam National University) ;
- Park, Jong-hyeon (Veterinary Research Institute) ;
- Hwang, Eui-kyung (Veterinary Research Institute) ;
- Huh, Won (Daesung Microbiological Laboratory)
- Received : 1994.07.26
- Published : 1994.07.30
Abstract
An attempt was made to isolate a causative viral agents from the fecal specimens of the diseased dogs with the gastroenteritis symptoms. Two coronavirus-like agents were isolated by serial dilution end point method and plaque assay. The isolates were characterized in terms of cytopathology, antigenicity, replication, physicochemical and morphological properties. The results obtained through the experiment were as follows; 1. Among 7 fecal specimens collected from the dogs with enteric disease, 2(28.6%)coronavirus-like agents showing typical cytopathic effects of canine coronavirus were isolated, and designated as CCV D1 and CCV D2, respectively. 2. By the cross-neutralization test and indirect immunofluoresence antibody test, the isolates were antigenically indentified as the standard CCV. The viruses were replicated only in the cytoplasm of A-72 cells. 3. The isolates showed no haemagglutinating activity against the erythrocytes from 11 kinds of animals. 4. The electron microscopic observation for the isolates showed spherical and pleomorphic features, covered with club-shaped projections on the surface. The size of particles was ranged from 70 to 150nm. 5. In one-step growth curve for the isolates in A-72 cells, maximum titers of intracellular vius was