• Plant Biotechnology Reports
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• v.3 no.4
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

• Journal of The Korean Association of Information Education
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• v.9 no.2
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• pp.281-298
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• 2005

It is needed to make more efforts to activate the gifted child education in elementary information science regarding selection of the gifted, development of appropriate curriculum, however, as a saying goes that a quality of education is depended on teacher's quality, and outstanding performance of teachers in charge of teaching the gifted is critical to make education for the gifted in elementary information science successful. Considering the significances of teacher variances, the research on the professionality of teacher who is responsible for the elite education in elementary information science should be made, along with a plan to cultivate their competence.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.13-13
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• 2017

By the progress of genome sequencing, infrastructures for marker-assisted breeding (MAB) of rice came to be established. Fine mapping and gene isolation have been conducted using the breeding materials derived from natural variations and artificial mutants. Such genetic analysis by the genome-wide dense markers provided us the knowledge about the many genes controlling important traits. We identified several genes or quantitative trait loci (QTL) for heading date, blast resistance, eating quality, high-temperature stress tolerance, and so on. NILs of each gene controlling heading date contribute to elongate the rice harvest period. Determination of precise gene location of blast resistance gene pi21, allowed us to overcome linkage drag, co-introduction of undesirable eating quality. We could also breed the first practical rice cultivar in Japan with a brown planthopper resistance gene bph11 in the genetic back-ground of an elite cultivar. Discovery of major and minor QTLs for good eating quality allowed us to fine-tune of eating quality according to the rice planting area or usage of rice grain. Many rice cultivars have bred efficiently by MAB for several traits, or by marker-assisted backcross breeding through chromosome segment substitution lines (CSSLs) using genetically diverse accessions. We are also systematically supporting the crop breeding of other sectors by MAB or by providing resources such as CSSLs. It is possible to pyramid many genes for important traits by using MAB, but is still difficult to improve the yielding ability. We are performing a Genomic Selection (GS) for improvement of rice biomass and grain yield. We are also trying to apply the genome editing technology for high yield rice breeding.

• Korean Journal of Plant Resources
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• v.32 no.3
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• pp.244-253
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• 2019

In crop breeding program, information about genetic dissimilarity on breeding resources is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations and inbred breeding. This study aimed to evaluate the genetic variation in Kenyan sunflower breeding lines based on simple sequence repeat (SSR). A total of 83 alleles were detected at 32 SSR loci. The allele number per locus ranged from 2 to 7 with an average of 2.7 alleles per locus detected from the 24 sunflower accessions and the average value of polymorphic information contents (PIC) were 0.384. A cluster analysis based on the genetic similarity coefficients was conducted and the 24 sunflower breeding resources were classified into three groups. The principal coordinates (PCoA) revealed 34% and 13.38% respectively, and 47.38% of total variation. It was found that the genetic diversity within the Kenyan sunflower breeding resources was narrower than that in other sunflower germplasm resources, suggesting the importance and feasibility of introducing elite genotypes from different origins for selection of breeding lines with broader genetic base in Kenyan sunflower breeding program.

• Journal of Animal Science and Technology
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• v.47 no.3
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• pp.355-362
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• 2005

This study was carried out to obtain basic information on variety selection for the utilization of whole crop rices(WCR) at National Institute of Crop Science, RDA, in 2004. Fifteen varieties and elite line were evaluated on feed value such as dry matter yield(DMY), crude protein( CP), acid detergent fiber(ADF), neutral detergent fiber(NDF) and total digestible nutrients(TDN). The dry matter yields were ranged from 13.23 to 17.83 ton per ha, the highest yielding varieties were Sobibyeo(l6.98ton / ha) in Japonica type, SR22060 (17.83 ton / ha) in New plant type, Hangangchalbyeo(I7.66 ton / ha) in Tongil type. Suweon 468 showed the highest value in the RFV and TDN content among the varieties, and Suweon 468, Suweon 498, Suweon 490 and SR22058 were chosen to have the high feed values through cluster analysis. The dry weight(grain) was found to be positively related with percent of the ripened grain, 1,000 grain weight and CPo TDN content was found to be positively related with CP, but negatively related with NDF and ADF. RFV was found to be negatively related with plant height, NDF and ADF. The promising rice varieties for WCR were Suweon 468, Suweon 498, Suweon 490 and SR22058 on the basis of CP, TDN and DMY.

• Journal of Animal Science and Technology
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• v.58 no.10
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• pp.38.1-38.6
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• 2016

Background: Despite the importance of relationships between somatic cell score (SCS) and currently selected traits (milk, fat and protein yield) of Holstein cows, there was a lack of comprehensive literature for it in Iran. Therefore we tried to examine heritabilities and relationships between these traits using a fixed-regression animal model and Bayesian inference. The data set consisted of 1,078,966 test-day observations from 146,765 primiparous daughters of 1930 sires, with calvings from 2002 to 2013. Results: Marginal posterior means of heritability estimates for SCS ($0.03{\pm}0.002$) were distinctly lower than those for milk ($0.204{\pm}0.006$), fat ($0.096{\pm}0.004$) and protein ($0.147{\pm}0.005$) yields. In the case of phenotypic correlations, the relationships between production and SCS were near zero at the beginning of lactation but become increasingly negative as days in milk increased. Although all environmental correlations between production and SCS were negative ($-0.177{\pm}0.007$, $-0.165{\pm}0.008$ and $-0.152{\pm}0.007$ between SCS and milk, fat, and protein yield, respectively), slightly antagonistic genetic correlations were found; with posterior mean of relationships ranging from $0.01{\pm}0.039$ to $0.11{\pm}0.036$. This genetic opposition was distinctly higher for protein than for fat. Conclusion: Although small, the positive genetic correlations suggest some genetic antagonism between desired increased milk production and reduced SCS (i.e., single-trait selection for increased milk production will also increase SCS).

• The Journal of Advanced Prosthodontics
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• v.5 no.2
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• pp.147-152
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• 2013

PURPOSE. A survey was performed to identify the level of mouthguard use, awareness, wearability issues and attitude toward mouthguard among elite Korean Taewondo athletes. MATERIALS AND METHODS. Survey questionnaires were given to 152 athletes participating in the Korea National Taekwondo team selection event for the 2010 Guangzhou Asian Games. Questionnaires consisted of three sections, mouthguard awareness, reasons for not wearing mouthguard and the last section to test the level of acceptance on current mouthguard and when the identified problems were resolved. For analyzing difference among response, ${\chi}^2$ test was used and significant level (${\alpha}$) was set up as 0.05. RESULTS. Responses in each of items showed significant difference (P<.001). Majority of response regarding each question: Majority of respondents believed that mouthguard were effective in preventing injuries (36.4%) but the result suggested that the provision of information on mouthguard to athletes was inadequate (44.0%) and the result showed that respondents were not greatly interested or concerned in relation to the mandatory mouthguard rule (31.6%). Although the responses on the level of comfort and wearability of mouthguard were negative (34.8%), athletes were positively willing to wear mouthguard if the problems rectified (51.2%). CONCLUSION. Considering the high level of willingness to wear mouthguard if the problems rectified, it is thought that together with efforts in providing more mouthguard information, the work of sports dentistry to research and improve mouthguard will be invaluable in promoting mouthguard to more athletes.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.12
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• pp.2343-2348
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• 2008

Ant algorithm is new meta heuristic for hard combinatorial optimization problem. It is a population based approach that uses exploitation of positive feedback as well as greedy search. It was first proposed for tackling the well known Traveling Salesman Problem. In this paper, we propose the improved $AS_{rank}$ algorithms. The original $AS_{rank}$ algorithm accomplishes a pheromone updating about only the paths which will be composed of the optimal path is higher, but, the paths which will be composed the optimal path is lower does not considered. In this paper, The proposed method evaporate the pheromone of the paths which will be composed of the optimal path is lowest(worst tour path), it is reducing the probability of the edges selection during next search cycle. Simulation results of proposed method show lower average search time and average iteration than original ACS.

• Korean Journal of Medicinal Crop Science
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• v.26 no.6
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• pp.471-476
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• 2018

Background: Astragalus membranaceus belonging to the Leguminosae family is often utilized as a traditional medicine. The aim of this study was to elucidate the basic breeding information required to develop short stem A. membranaceus cultivars. Methods and Results: Roots of A. membranaceus advanced yield trial (AYT) lines were harvested in late October 2017. Root yield of six AYT lines were increased in a range of 8.9 - 74.8% compared with 'Aseong' as control (check variety). The height of seven AYT lines were shorter than that of 'Aseong'. In addition, stem diameter of nine AYT lines was thicker than that of 'Aseong'. Consequently, 1502-56, 1503-90, and 1510-80 were selected as elite lines for the development of short stem cultivars. HPLC analysis was perfromed to identify lines with high level active components such as calycosin-7-O-${\beta}$-D-glucoside and calycosin. The levels of both active components were higher in 1502-56, and 1503-90, but not in 1510-80 compared to 'Aseong'. In addition, 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity was higher in the 1502-56 compared to 'Aseong'. Considering these results, two AYT lines, 1502-56 and 1503-90 were selected as short stem lines with high calycosin-7-O-${\beta}$-D-glucoside and calycosin content. Conclusions: Taken together, Two short stem lines were identified in this study. In our future study, regional yield trial (RYT) will be conducted using these selected lines to develop new cultivars.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.24 no.2
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• pp.186-191
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• 2020

In this paper, we proposed an optimization technique for efficiently placing vehicles on decks in a vehicle-carrying ship to efficiently handle loading and unloading. For this purpose, we utilized the transformation method of the XML data representing the ship's spatial information, merging and branching algorithm and genetic algorithm, and implemented the function to visualize the optimized vehicle placement results. The techniques of selection, crossover, mutation, and elite preservation, which are used in the conventional genetic algorithms, are used. In particular, the vehicle placement optimization method is proposed by merging and branching the ship space for the vehicle loading. The experimental results show that the proposed merging and branching method is effective for the optimization process that is difficult to optimize with the existing genetic algorithm alone. In addition, visualization results show vehicle layout results in the form of drawings so that experts can easily determine the efficiency of the layout results.