Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Selection of Short Stem Astragalus membranaceus Lines by Assessing Agronomic Characteristics and Biological Activity

농업형질 및 생리활성 평가를 통한 황기 단간 계통 선발

  • 이상훈 (농촌진흥청 국립원예특작과학원) ;
  • 구성철 (농촌진흥청 국립원예특작과학원) ;
  • 한종원 (농촌진흥청 국립원예특작과학원) ;
  • 이우문 (농촌진흥청 국립원예특작과학원) ;
  • 허목 (농촌진흥청 국립원예특작과학원)
  • Received : 2018.10.25
  • Accepted : 2018.12.17
  • Published : 2018.12.30
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Abstract

Background: Astragalus membranaceus belonging to the Leguminosae family is often utilized as a traditional medicine. The aim of this study was to elucidate the basic breeding information required to develop short stem A. membranaceus cultivars. Methods and Results: Roots of A. membranaceus advanced yield trial (AYT) lines were harvested in late October 2017. Root yield of six AYT lines were increased in a range of 8.9 - 74.8% compared with 'Aseong' as control (check variety). The height of seven AYT lines were shorter than that of 'Aseong'. In addition, stem diameter of nine AYT lines was thicker than that of 'Aseong'. Consequently, 1502-56, 1503-90, and 1510-80 were selected as elite lines for the development of short stem cultivars. HPLC analysis was perfromed to identify lines with high level active components such as calycosin-7-O-${\beta}$-D-glucoside and calycosin. The levels of both active components were higher in 1502-56, and 1503-90, but not in 1510-80 compared to 'Aseong'. In addition, 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity was higher in the 1502-56 compared to 'Aseong'. Considering these results, two AYT lines, 1502-56 and 1503-90 were selected as short stem lines with high calycosin-7-O-${\beta}$-D-glucoside and calycosin content. Conclusions: Taken together, Two short stem lines were identified in this study. In our future study, regional yield trial (RYT) will be conducted using these selected lines to develop new cultivars.

Keywords

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Fig. 1. Selected chromatogram of calycosin-7-O-β-D-glucoside (A) calycosin (B) and sample (C) from A. membranaceus.

Table 1. LC analyisis condition of calycosin, calycosin-7-O-?-Dglucoside, in A. membranaceus.

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Table 2. Agronomic characteristics of A. membranaceus lines.

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Table 3. Calycosin-7-O-β-D-glucoside and calycosin contents of A. membranaceus lines.

OOJJBO_2018_v26n6_471_t0003.png 이미지

Table 4. DPPH free radical scavenging activity of A. membranaceus lines.

OOJJBO_2018_v26n6_471_t0004.png 이미지

References

  1. Baek NI, Kim YS, Kyung JS and Park KH. (1996). Isolation of anti-hepatotoxic agent from the root of Astragalus membranaceus. Korean Journal of Pharmacognosy. 27:111-116.
  2. Choe ME, Kim JI, Jung TW, Kwak DY, Kim KY, Ko JY, Woo KS, Song SB, Jung KY and Oh IS. (2016). Waxy sorghum(Sorghum bicolor L.) variety 'Nampungchal' with lodging resistant and high yield. Korean Journal of Breeding Science. 48:192-197. https://doi.org/10.9787/KJBS.2016.48.2.192
  3. Goh EJ, Seong ES, Lee JG, Na JK, Lim JD, Kim MJ, Kim NY, Lee GH, Seo JS, Cheoi DS, Chung IM and Yu CY. (2009). Antioxidant activites according to peeling and cultivated years of Astragalus membranaceus roots. Korean Journal of Medicinal Crop Science. 17:233-237.
  4. Hwang EY, Kim DH, Kim HJ, Hwang JY, Park TS, Lee IS and Son JH. (2011). Antioxidant activities and nitric oxide production of medicine plants in Gyeongsangbukdo(Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora). Journal of Applied Biological Chemistry. 54:171-177. https://doi.org/10.3839/jabc.2011.029
  5. Jeon MR, Yoo JH, Kim CH, Choi JH, Kang BJ, Seong ES and Yu CY. (2016). Selection of superior sorghum accession by assessing agronomic characters and biological activity. Korean Journal of Medicianl Crop Science. 24:386-392. https://doi.org/10.7783/KJMCS.2016.24.5.386
  6. Jiang K, Lu Q, Li Q, Ji Y, Chen W and Xue X. (2017). Astragaloside IV inhibits breast cancer cell invasion by suppressing Vav3 mediated Rac1/MAPK signaling. International Immunopharmacology. 42:195-202. https://doi.org/10.1016/j.intimp.2016.10.001
  7. Ju JI, Kim SD, Park YC, Lee BH, Seo JS, Nam YG, Paik SW and Kim HH. (2015). A chinese matrimony vine(Lycium chinense Miller) cultivar with many branches and high ingredients 'Cheongkwang'. Korean Journal of Breeding Science. 47:318-323. https://doi.org/10.9787/KJBS.2015.47.3.318
  8. Jung HS, Lee EJ, Lee JH, Kim JS and Kang SS. (2008). Phytochemical studies on root Astragalus root(3)-triterpenoids and sterols. Korean Journal of Pharmacognosy. 39:186-193.
  9. Kim DH, Park CH, Park HW, Park CG, Sung JS, Yu HS, Kim GS, Seong NS, Kim JC, Kim MS, Bae SG and Chung BJ. (2008). A new high-quality, disease resistance and high-yielding Rehmannia glutinosa cultivar, "Kokang". Korean Journal of Breeding Science. 40:84-87.
  10. Kim JS and Kim C. (1997). A study on the constituents from the roots of Astragalus membranaceus(II). Korean Journal of Pharmacognosy. 28:75-79.
  11. Kim JS, Kim YT and Kim CS. (1996). A study on the constituents from the roots of Astragalus membranaceus(I). Korean Journal of Pharmacognosy. 27:336-341.
  12. Kim NS, Choi DJ, Choi EJ, Lee JH, Park S, Lee YS, Lee JW, Lee DY, Kim GS and Lee SE. (2018). Screening and evaluation of the anti-allergic effect of Korean medicinal plant extracts. Korean Journal of Medicinal Crop Science. 26:42-54.
  13. Kim SH, Jun YM, Lim JJ, Kim SH, Chung IM and Kim EH. (2012). Variation of astragalosides contents in cultivated Astragalus membranaceus. Korean Journal of Medicinal Crop Science. 20:372-380. https://doi.org/10.7783/KJMCS.2012.20.5.372
  14. Kong L, Li X, Wang H, He G and Tang A. (2018). Calycosin inhibits nasopharyngeal carcinoma cells by influencing EWSAT1 expression to regulate the TRAF6-related pathways. Biomedicine and Pharmacotherapy. 106:342-348. https://doi.org/10.1016/j.biopha.2018.06.143
  15. Lee HJ, Lee SW, Park CG, Ahn YS, Kim JS, Bang MS, Oh CH and Kim CT. (2015). Effects of white Habiscus syriacus L. flowe extracts on antioxidant activity and bone resorption inhibition. Korean Journal of Medicinal Crop Science. 23:190-197. https://doi.org/10.7783/KJMCS.2015.23.3.190
  16. Li C, Yang F, Liu F, Li D and Yang T. (2018). NRF2/HO-1 activation via ERK pathway involved in the anti-neuroinflammatory effect of astragaloside IV in LPS induced microglial cells. Neuroscience Letters. 666:104-110. https://doi.org/10.1016/j.neulet.2017.12.039
  17. Ministry of Agriculture, Food and Rural Affairs(MAFRA). (2018). 2017 an actual output of crop for a special purpose. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea. p.7.
  18. Park YC, Lee JS, Kim DY, Son HY, Lee JW, Cheoi YS, Kim KK, Yu CY, Chung IM, Im MH, Lee KJ, Choi RN, Shim HS and Lim JD. (2013). A 90 day repeated dose-oral toxicity study of extracts from Astragalus membranaceus-aboveground parts in rats. Korean Journal of Medicinal Crop Science. 21:474-485. https://doi.org/10.7783/KJMCS.2013.21.6.474
  19. Park YS. (2002). Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. Journal of the East Asian Society of Dietary Life. 12:23-31.
  20. Qin CD, Ma DN, Ren ZG, Zhu XD, Wang CH, Wang YC, Ye BG, Cao MQ, Gao DM and Tang ZY. (2017). Astragaloside IV inhibits metastasis in hepatoma cells through the suppression of epithelial-mesenchymal transition via the Akt/GSK-$3{\beta}/{\beta}$-catenin pathway. Oncology Reports. 37:1725-1735. https://doi.org/10.3892/or.2017.5389
  21. Rios JL and Waterman PG. (1997). A review of the pharmacology and toxicology of Astragalus. Phytotherapy Research. 11:411-418. https://doi.org/10.1002/(SICI)1099-1573(199709)11:6<411::AID-PTR132>3.0.CO;2-6
  22. Song BK, Lee EJ, Kim HK, Jin SD, Kim SJ and Kim DH. (1998). Effect of Astrgali radix on the function of murine immunocytes in vivo and in vitro. Korea Journal of Herbology. 13:115-128.
  23. Sung JS, Jeong YJ, Kim DJ, Assefa AD, Jeon YA, Hur OS, Ro NY, Ko HC, Ok HC, Rhee JH, Lee MC and Baek HJ. (2018). Oil compositions and antioxidant properties of safflower germplasm collected from East Asia. Korean Journal of Medicinal Crop Science. 26:32-41.
  24. Wang S, Mou J, Cui L, Wang X and Zhang Z. (2018). Astragaloside IV inhibits cell proliferation of colorectal cancer cell lines through down-regulation of B7-H3. Biomedicine and Pharmacotherapy. 102:1037-1044. https://doi.org/10.1016/j.biopha.2018.03.127
  25. Xia L, Guo D and Chen B. (2017). Neuroprotective effects of astragaloside IV on parkinson disease models of mice and primary astrocytes. Experimental and Therapeutic Medicine. 14:5569-5575.
  26. Yang SJ, Lee SY, Lee H, Park YC, Choi SK, Yu CY, Chung IM and Lim JD. (2016). Adjuvant effect of polysaccharides from aboveground parts of Astragalus membranaceus. Korean Journal of Medicinal Crop Science. 24:408-419. https://doi.org/10.7783/KJMCS.2016.24.5.408
  27. Yin Y, Heo SI, Jung MJ and Wang MH. (2009). Antioxidant and antidiabetic effects of various sections of Astragalus membranaceus. Korean Journal of Pharmacognosy. 40:1-5.