• Mycobiology
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• 제43권3호
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• pp.184-194
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• 2015

A compressive description of tropical milky white mushroom (Calocybe indica P&C var. APK2) is provided in this review. This mushroom variety was first identified in the eastern Indian state of West Bengal and can be cultivated on a wide variety of substrates, at a high temperature range ($30{\sim}38^{\circ}C$). However, no commercial cultivation was made until 1998. Krishnamoorthy 1997 rediscovered the fungus from Tamil Nadu, India and standardized the commercial production techniques for the first time in the world. This edible mushroom has a long shelf life (5~7 days) compared to other commercially available counterparts. A comprehensive and critical review on physiological and nutritional requirements viz., pH, temperature, carbon to nitrogen ratio, best carbon source, best nitrogen source, growth period, growth promoters for mycelia biomass production; substrate preparation; spawn inoculation; different supplementation and casing requirements to increase the yield of mushrooms has been outlined. Innovative and inexpensive methods developed to commercially cultivate milky white mushrooms on different lignocellulosic biomass is also described in this review. The composition profiles of milky white mushroom, its mineral contents and non-enzymatic antioxidants are provided in comparison with button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus ostreatus). Antioxidant assay results using methanol extract of milky white mushroom has been provided along with the information about the compounds that are responsible for flavor profile both in fresh and dry mushrooms. Milky white mushroom extracts are known to have anti-hyperglycemic effect and anti-lipid peroxidation effect. The advantage of growing at elevated temperature creates newer avenues to explore milky white mushroom cultivation economically around the world, especially, in humid tropical and sub-tropical zones. Because of its incomparable productivity and shelf life to any other cultivated mushrooms in the world, milky white mushroom could play an important role in satisfying the growing market demands for edible mushrooms in the near future.

• 한국식품저장유통학회지
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• 제6권4호
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• pp.506-513
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• 1999

유기게르마늄(Ge-132, carboxyethylgermanium)에 의한 22가지 젖산균의 성장 효과를 0.01~10mg/m1의 농도로 첨가된 GPYS 액체배지에서 조사하였다. 시험한 대부분의 젖산균은 고농도의 게르마늄에서도 내성이 있었고, 게르마늄의 농도가 높을수록 성장을 더욱 촉진시키는 효과가 나타났다. 게르마늄이 10mg/m1의 농도로 첨가된 GPYS배지에서 Lactococcus factis, Lc. cremoris, Lc. diacetilactis, Enterococus faecium 및 Streptococcus faecalis는 2배 이상 생육촉진의 효과를 나타내었으나, Leuconostoc mesenteroides와 Pediococcus pentosareus는 저해를 나타내었다. Lc. lactic와 Lc. cremoris의 경우, 배양액의 점도는 게르마늄이 첨가된 GPYS배지에서 급격히 증가되었지만, 장시간 배양에 의해서는 약간 감소되었다. 그러나 Lc. diacetilactis, E. faecium와 S. faecalis의 경우, 게르마늄의 첨가에 의하여 생육은 현저하게 촉진되었지만, 배양중의 점도는 증가되지 않았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.267-267
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• 2017

In Korea, the average air temperature has been elevated twice faster than the average global warming. And the climate warming is characterized by the smaller rise of air temperature in summer and the greater rise of air temperature in spring and winter. Therefore, the number of frost-free days to determine the cultivation ability of crops has increased by more than 15 days in 10 years according to climate warming. This climate warming trend has extended and is projected to extend not only the sweet potato growing season but also the sweet potato early cultivating area to higher altitude and latitude region. This study was carried out to evaluate the possibility of sweet potato double cropping in the southern island area of Korea by assessing the growth and yield performance of sweet potato cultivated at extremely-early and -late time. We had performed at Yokji Island Yokji Island($E128^{\circ}$ 18' $N34^{\circ}$ 36'), a representative specified complex area of sweet potato cultivation in southern Korea. As the test varieties, the major cultivars of the this region, Shinyulmi and early hypertrophic cultivars, Dahomi were used. The prior cropping were planted with PE film mulching on March 30 and April 10, and harvested after 110 days. So the succeeding cropping were planted without PE film mulching on July 25 and August 5 according to the harvesting time of the prior sweet potato and harvested after 120 days. As a control, it was harvested on September 15, 120 days after planted on May 15. Each experimental plot had an area of 12 square meters consisting of 4 beds, and was planted one at a time at intervals of 25cm. We had investigated growth characteristics - main vine length, node number, branch number, total vine yield, and tuberous root characteristics - tuberous root number, average weight, starch value, and etc. After harvesting, we analyzed the economic effects by examining the postharvest quantity, the input labor, the management cost, and the income. The total yield of marketable products in prior and succeeding cropping was 46~70% higher than that of control. The average unit price of sweet potato was 36% higher than the conventional culture, and the gross income increased by 98%, but the operating cost increased by 83%, and the farm income increased by 103%. There are considerations such as the difficulty of enlargement of cultivation area due to lack of labor in limited space and the need for watering measures due to spring drought. However, if the area of application for sweet potatoes double system is increased by 10%, it can be used as a new cropping system.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.280-280
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• 2017

In Korea, the average air temperature has been elevated twice faster than the average global warming. And the climate warming is characterized by the smaller rise of air temperature in summer and the greater rise of air temperature in spring and winter. Therefore, the number of frost-free days to determine the cultivation ability of crops has increased by more than 15 days in 10 years according to climate warming. This climate warming trend has extended and is projected to extend not only the sweet potato growing season but also the sweet potato early cultivating area to higher altitude and latitude region. This study was carried out to evaluate the possibility of sweet potato double cropping in the southern island area of Korea by assessing the growth and yield performance of sweet potato cultivated at extremely-early and -late time. We had performed at Yokji Island Yokji Island($E128^{\circ}$ 18' $N34^{\circ}$ 36'), a representative specified complex area of sweet potato cultivation in southern Korea. As the test varieties, the major cultivars of the this region, Shinyulmi and early hypertrophic cultivars, Dahomi were used. The prior cropping were planted with PE film mulching on March 30 and April 10, and harvested after 110 days. So the succeeding cropping were planted without PE film mulching on July 25 and August 5 according to the harvesting time of the prior sweet potato and harvested after 120 days. As a control, it was harvested on September 15, 120 days after planted on May 15. Each experimental plot had an area of 12 square meters consisting of 4 beds, and was planted one at a time at intervals of 25cm. We had investigated growth characteristics - main vine length, node number, branch number, total vine yield, and tuberous root characteristics - tuberous root number, average weight, starch value, and etc. After harvesting, we analyzed the economic effects by examining the postharvest quantity, the input labor, the management cost, and the income. The total yield of marketable products in prior and succeeding cropping was 46~70% higher than that of control. The average unit price of sweet potato was 36% higher than the conventional culture, and the gross income increased by 98%, but the operating cost increased by 83%, and the farm income increased by 103%. There are considerations such as the difficulty of enlargement of cultivation area due to lack of labor in limited space and the need for watering measures due to spring drought. However, if the area of application for sweet potatoes double system is increased by 10%, it can be used as a new cropping system.

• 미생물학회지
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• 제44권4호
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• pp.352-357
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• 2008

난배양성 세균의 배양효율을 증진시킬 수 있다고 보고된 배지 첨가물들이 포함된 다양한 종류의 빈영양 배지들을 대상으로 배양효율을 비교평가하고 최적의 배양조건을 모색하였으며, 평가된 배지를 사용하여 토양시료로부터 순수 분리된 난배양성 세균들의 계통분류학적 위치를 분석하였다. 배지 첨가물로는 토양의 화학적 조성을 반영하기 위한 토양추출액(soil extract), 부식질산의 유사체(humic acid analogue)인 anthraquinone disulfonate, 정족수인식 신호물질(quorum-signaling compounds)인 acyl homoserine lactones, 과산화물(exogenous peroxide)로부터 세균을 보호하기 위한 catalase가 사용되었다. $CO_2$ 과분압(5%, v/v) 조건에서 60일간 배양하였을 때, catalase가 첨가된 배지가 가장 높은 세균집락수(CFU)를 보였다. 이 배지로부터 147개의 균주를 무작위적으로 선택하여 순수분리하고 16S rRNA 유전자의 염기서열을 이용한 계통학적 분석을 실시한 결과, 순수분리된 균주의 약30%가 이전에 배양 또는 발견된 적이 없는 새로운 종(species)에 속하며, 이 중 약 25%는 새로운 과(family)에 속하는 세균일 가능성이 있는 것으로 나타났다. 또한 난배양성 토양세균으로 알려진 phylum Acidobacteria에 속하는 세균들이 성공적으로 배양되었다는 결과를 고려하면, 본 연구에서 사용된 배지 및 배양조건은 난배양성 토양세균의 배양은 물론 신 분류군의 발굴에도 큰 기여를 할 것으로 기대된다.

• Journal of Microbiology
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• 제37권2호
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• pp.90-96
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• 1999

The core protein of the hepatitis C virus (HCV) is a multifunctional protein. The HCV core protein was reported to regulate cellular gene expression and transform primary rat embryo fibroblast cells. However, the role of the core protein in the pathogenesis of HCV-associated liver diseases is not well understood. To investigate the functional role of the core protein in cytophathogenicity, we have constructed stable expression systems of full length or truncated HCV core protein lacking the C-terminal hyderophobic domains and established HepG2 cell clones constitutively expressing the core protein. The full length core protein was localized in the cytoplasm and the C-terminal truncated core protein was localized in the nucleus. HepG2 cells expressing nuclear, truncated core protein showed elevated cell death during cultivation compared to untransfected cells and full length core-expressing cells. In the treatment with bleomycin, both cell clones expressing full length or truncated core protein appeared to be more sensitive to blemoycin than the parental HepG2 cells. These results suggest that the core protein may play a role in HCV pathogenesis promoting apoptotic cell death of infected cells.

• 한국미생물·생명공학회지
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• 제18권1호
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• pp.66-70
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• 1990

부피질호르몬의 제조의 중요한 전구체인 11alpha-hydroxyprogesterone을 Rhizopus nigricans를 이용하여 고농도의 progesterone으로부터 고수율로 생산하기 위한 연구를 수행하였다. 생물전환수율을 최대로 하는 배지조성은 포도당과 casamino acid의 비율이 2:1인 것으로 관찰되었다. Progesterone은 Tween 80 용액에 분산시켜서 종균배양 후 12 시간 정도 지나서 첨가하였을 때 전환수율이 높았다. Progesterone의 농도가 증가함에 따라 생산성이 증가하였고 Progesterone이 20g/$\ell$일 경우 전환수율은 약 70정도 였다.

• 한국농림기상학회지
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• 제20권2호
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• pp.175-182
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• 2018

본 연구는 기온에 따른 봄배추 '춘광'의 재배 적기를 밝혀 농가에 적정 재배시기를 제공하기 위해서 수행되었다. 기온이 외기보다 $6^{\circ}C$까지 단계적으로 상승하도록 설계된 온도구배 터널을 3개의 구역(외기+$2^{\circ}C$, A; 외기+$4^{\circ}C$, B; 외기+$6^{\circ}C$, C)으로 나눈 후 작기(2017.3.6., 1차; 3.20., 2차; 4.3., 3차)에 걸쳐 정식하였다. 재배기간 동안의 생육도일(growing degree days, GDDs)은 1차 A, 1차 B, 2차 A, 1차 C, 2차 B, 3차 A, 2차 C, 3차 B, 3차 C 순으로 높았다. 구중은 1차 B, 1차 C, 2차 A 처리구에서 높게 나타나 처리간 높은 유의적 차이를 보였으나 생태적인 특성(엽수, 엽면적, 최대엽의 엽장 및 엽폭 등)은 차이가 없었다. 다만, 생육초기 $13^{\circ}C$ 이하의 저온에 감응하였던 결과로 1차 A 처리구에서 5.5%의 추대현상이 발생하였다. 또한 2차 C, 3차 A, B, 및 C 처리구에서 기온이 높아짐에 따라 각각 11.0, 5.5, 33.3, 및 44.4%의 속썩음 현상이 발생하였다. GDDs에 따른 배추 구중은 587 GDDs까지 증가하다 729 GDDs 이후에는 오히려 감소하였다. GDDs에 따른 배추 수량과 적정 재배시기 예측식에서 최대 기대수량은 정식 후 64일 기준 GDDs가 601일 때 16.3MT/10a이었다. 최대 수량의 95%(약 15.5MT/10a) 이상을 재배 적기로 설정할 때 적정 범위는 478~724 GDDs로 산출되었다. 재배적기 예측식의 검증 결과, 봄배추 주산지인 진도, 해남, 나주, 서산, 평택의 GDDs는 각각 634, 619, 666, 652, 및 719이었고, 예측식에서 산출된 범위(478~724 GDDs)에 포함되었다. 결과적으로 봄배추의 재배 최적기는 예측식의 최대값인 수확기준 601 GDDs이며 적정 범위는 호냉성인 온도 특성을 감안하여 478~724 GDDs인 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.637-645
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• 2011

Production of recombinant proteins by excretory expression has many advantages over intracellular expression in Escherichia coli. Hyperexpression of a secretory exoglucanase, Exg, of Cellulomonas fimi was previously shown to saturate the SecYEG pathway and result in dramatic cell death of E. coli. In this study, we demonstrated that overexpression of the PspA in the JM101(pM1VegGcexL-pspA) strain enhanced excretion of Exg to 1.65 U/ml using shake-flask cultivation, which was 80% higher than the highest yield previously obtained from the optimized JM101(pM1VegGcexL) strain. A much higher excreted Exg activity of 4.5 U/ml was further achieved with high cell density cultivation using rich media. Furthermore, we showed that the PspA overexpression strain enjoyed an elevated critical value (CV), which was defined as the largest quotient between the intracellular unprocessed precursor and its secreted mature counterpart that was still tolerable by the host cells prior to the onset of cell death, improving from the previously determined CV of 20/80 to the currently achieved CV of 45/55 for Exg. The results suggested that the PspA overexpression strain might tolerate a higher level of precursor Exg making use of the SecYEG pathway for secretion. The reduced lethal effect might be attributable to the overexpressed PspA, which was postulated to be able to reduce membrane depolarization and damage. Our findings introduce a novel strategy of the combined application of metabolic engineering and construct optimization to the attainment of the best possible E. coli producers for secretory/excretory production of recombinant proteins, using Exg as the model protein.

• 한국작물학회지
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• 제64권3호
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• pp.165-175
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• 2019

중부 평야지에서 온난화 현황을 반영하여 조생종 벼의 이앙시기에 따른 완전미 수량의 변화를 검토하고, 완전미 수량 향상과 관련된 작물학적 특성을 분석하기 위하여, 2017~2018년 2년에 걸쳐 국립식량과학원 중부작물부(수원) 벼 재배시험 포장에서 6품종을 이용하여 시험한 결과는 다음과 같다. 1. 완전미 무게는 품종에 따라 6월 14일~29일 이앙, 6품종 공통 6월 29일 이앙에서 통계적으로 가장 높았는데, 완전미 최고 무게를 나타낸 출수 후 40일간 등숙적온은 품종에 따라 $21.3{\sim}23.9^{\circ}C$, 6품종 평균 $21.8^{\circ}C$이었다. 2. 완전미 무게는 쌀 무게보다 완전립 비율과 더 밀접한 상관관계를 나타내었다. 3. 출수 후 40일간 등숙적온은 쌀 무게 기준에서 $23.5^{\circ}C$, 완전립 비율 기준에서 $20.5^{\circ}C$로, 쌀수량보다 품위 향상을 위한 등숙적온이 $3^{\circ}C$ 낮게 나타났다. 4. 완전미 무게 향상에 일정 부분 기여한 쌀 무게도 6품종 공통 6월 29일 이앙에서 가장 높았는데, 이에는 등숙비율과 $m^2$ 당 영화수의 증가가 기여하였다. 5. 오대 1품종을 이용하여 별도의 이앙시기 처리에서 검토하였을 때, 쌀수량이 가장 높았던 이앙시기에 $m^2$ 당 영화 수, 출수기 및 수확기 건물중, 수확지수가 통계적으로 가장 높은 수준이었다. 6. 결론적으로, 중부 평야지에서 조생종의 완전미 수량 향상을 위한 이앙적기는 품종에 따라 6월 14일~29일이었으며, 완전미 수량 향상의 작물학적 요인은 출수기 건물 중 증가에 따른 $m^2$ 당 영화수의 증대와 수확기 건물중 및 수확지수 증가에 따른 등숙비율의 향상, 그리고 완전립 비율 향상으로 분석되었다.