• 제목/요약/키워드: electrophoretic-velocity

검색결과 14건 처리시간 0.022초

매립지 침출수 화학종에 따른 점토입자의 전기영동 특성 (Electrophoretic Characteristics of the Clay Particles Affected by Chemical Species of Leachate)

  • 김종윤;한상재;김수삼;박재만
    • 대한토목학회논문집
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    • 제29권5C호
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    • pp.217-228
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    • 2009
  • 실제 폐기물 매립지의 누출 복원을 위해 전기영동기법을 적용할 경우, 핵심적으로 우선 고려해야 할 사항은 실제 침출수 전해질 환경에서 전기영동력에 의한 입자의 이동 및 누출구 복원 가능성을 판단하는 것이다. 하지만 침출수의 다양한 화학적 성분과 점토 입자와의 상호작용에 의한 점토입자들의 응집현상은 전기영동기법의 적용시키는데 큰 문제를 유발시킨다. 이에 따라 본 연구에서는 점토 입자의 응집에 영향을 미치는 침출수내 화학종과 그 범위를 파악하고자 1차원 중력침강실험과 전기영동 실험을 수행하였다. 또한 각각의 침강속도와 제타포텐셜, 전기영동적 이동속도 개념을 적용하여 침출수의 화학종과 그 농도에 따른 입자 거동 특성을 분석하였다.

Evaluation of Electrokinetic Flow Mobility Using Isotacho-Electrophoresis Techniques

  • An, J.H.;Joo, Y.H.;Lee, C.Y.;Lee, Y.J.;Park, C.W.
    • Journal of Magnetics
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    • 제16권4호
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    • pp.444-448
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    • 2011
  • In the present study, we separated the marker particles from the suspending particle mixture solution using isotacho-electrophoresis technique, a novel quantitative ionic particle separation method, in the microchannel. A multiple stacking zone of the suspending particle was visualized with variations in electric field strength, pH value and concentration of the ionic solution. In particular, the electrophoretic mobility of ionic particle (fluorescein) was estimated based on the electrophoretic velocity value measured by the particle image velocimetry. As a result, isotacho-electrophoresis zones were clearly visualized as going downstream in the electric field. The particle migration velocity increased proportional to the applied voltage increase; it was also affected by the pH value variations in the ionic solution.

나노 세공을 통한 비드 체인의 전기영동에 관한 수치해석적 연구 (NUMERICAL STUDY ON ELECTROPHORETIC MOTION OF A BIO-POLYMER THROUGH A NANO-PORE)

  • 알라파티 수레수;서용권
    • 한국전산유체공학회:학술대회논문집
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    • 한국전산유체공학회 2010년 춘계학술대회논문집
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    • pp.575-580
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    • 2010
  • In this work, the electrophoretic motion of dsDNA molecule represented by a polymer through an artificial nano-pore in a membrane is simulated using the numerical method combining the lattice Boltzmann and Langevin molecular dynamic method. The polymer motion is represented by Langevin molecular dynamics technique while the fluid flow is taken into account by fluctuating lattice-Boltzmann method. The hydrodynamic interactions between the polymer and solvent in a confined space with a membrane having a hole are considered explicitly through the frictional and the random forces. The electric field intensity over the space is obtained from a finite difference method. Initially, the polymer is placed at one side of the space, and an electric field is applied to drive the polymer to the other side of the space through the nano-pore. In future, we plan to study the effect of the polymer size and the electric field on the electrophoretic velocity.

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Separation of $\Phi$X HAE III DNA with Electrochromatography

  • Park, Young G.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제5권5호
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    • pp.332-339
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    • 2000
  • Experimental and theoretical works were performed for the separation of large polyelectrolytes such as DNA in the column packed with gel particles under an electric field. This paper shows how intraparticle convection effects the separation of DNAs in the column because DNAs quickly oriented through the pores in the field direction. Dimensionless transient mass balance equations were derived considering diffusion and electrophoretic convection. The separation criteria is theoretically studied using two different Peclet numbers in the fluid and solid phases and these criteria were verified uing two different DNAs by electrophoretic mobilities measured experimentally, showing how the separation position of DNAs varies in the column according to values of Pe(sub)f/Pe(sub)g of individual DNA. Governing equations are simultaneously solved by operator theoretic and characteristic methods to yield the column response.

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전계가 가해진 절연유의 유동대전 특성 고찰 (Study on the Streaming Electrification of Insulating oil Under Electricfield)

  • 허창수;정중일
    • E2M - 전기 전자와 첨단 소재
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    • 제10권4호
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    • pp.334-341
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    • 1997
  • Streaming electrification on insulating paper and pressboard under D.C. and A.C. electric field was investigated by using paper tubes and oil circulation apparatus. At first, flowing of static charges as measured with no electric field. As the temperature of oil increased, the measured current curve hows peak. As the velocity increased, it shows increasing exponential curve. Then, we applied A.C. and D.C. electric field on paper tube and the current from relaxation tank to earth was measured, which other factors such as temperature and velocity were varied like case of no electric field. The ions in oil carry the charges. So electric field makes asymmetry effect, and electrophoretic effect on ions in oil. We find that as the electric field intensity increased, the charges which were made by electric double layer were increased. The charge vs. velocity curve made peak point at a velocity.

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Residence Time Distribution in the Chromatographic Column: Applications in the Separation Engineering of DNA

  • Park, Young G.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권2호
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    • pp.117-125
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    • 2003
  • Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

Disc Electrophoresis에 의한 면화의 Genome과 종간관계 분석 (Analysis of Genome and Species Relationships in Cotton by Disc Electrophoresis)

  • 최종열;장병호
    • 한국작물학회지
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    • 제13권
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    • pp.73-76
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    • 1973
  • Protein spectra from 6 species of Gossypium were obtained by disc electraphoresis of seed extracts. Protein extracts were made by soaking 0.5g. of seed in 15ml of Tris-glycine buffer for 24 hours. Gels 24 hours. Gels were stained in 0.5% Amido Black solution for 1 hour, and destained in 7% acetic acid for 72 hours. Nine to 15 bands were visible in each gel. Homologies of Protein bands among the species were determined by migration velocity. Evidences obtained from electrophoretic separation of seed Protein were consistent with those from genetic, cytological, morphological and Phenogenetic methods regarding the origin of New World cultivated cottons. Possibility, however, does not exist to exclude Gossypium herbaceum from one of the Progenitors of New World cultivated cottons from electrophoretic evidences alone.

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전기막 시스템에서 유속과 농도에 따른 형광염료의 농축 및 전기영동 이동도에 관한 연구 (Studies on Preconcentration and Electrophoretic Mobility of Fluorescent Dyes Depending on Flow Velocity and Concentration in the Electromembrane System)

  • 김민성;김범주
    • 공업화학
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    • 제34권1호
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    • pp.45-50
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    • 2023
  • 마이크로 유체 시스템을 활용한 농축 기술은 저과다 분석물을 특정 위치에 모으거나 추출하는 기술로, 의료 및 바이오 분야를 포함한 다양한 분야에서 필수적인 기술로 각광받고 있다. 본 연구에서는 이온교환막을 활용한 전기막 시스템(electromembrane system)에서 전기영동(electrophoresis) 현상을 이용해 타겟 샘플을 농축할 때 고려해야 할 변수에 대한 광범위한 연구를 수행하였다. 가시화가 용이한 형광염료로 음전하를 띄는 Alexa Fluor 488과 양전하를 띄는 Rhodamine 6G을 샘플로 사용하여, 타겟 샘플이 포함된 메인 채널의 유속과 메인/버퍼 채널의 농도, 전압 등이 샘플 농축에 어떻게 영향을 끼치는지 알아보았다. 실험 결과, 메인/버퍼 채널 농도비가 같을 경우, 유속이 느릴수록, 샘플이 포함된 메인 채널의 농도가 높을수록, 타겟 샘플의 농축이 훨씬 더 잘 일어난다는 사실을 알 수 있었다. 또한 본 연구를 통해 Alexa Fluor 488과 Rhodamine 6G의 전기영동 이동도 값을 실험적으로 계산하여 비교하였다.

전기영동형 전자종이를 위한 $TiO_2$ 나노분말의 분산 제어 (A control dispersion of $TiO_2$ nano powder for electronic paper of electrophoresis)

  • 김중희;오효진;이남희;황종선;김선재
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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    • pp.324-327
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    • 2005
  • An electrophoretic display using $TiO_2$ particles is the most promising candidate because it offers various advantages such as ink-on-paper appearance, good contrast ratio, wide viewing angle, image stability in the off-state and extremely low power consumption. The core technology of electrophoretic display is the dispersion controlling of $TiO_2$ nano particles in nonaqueous solution. To prepare an ink for electronic paper using electrophoretic properties of $TiO_2$ nano particles, cyclohexane with low dielectric constant and transparency, polyethylene for producing polymer coating layer which reduces apparent gravity of $TiO_2$, and $TiO_2$ powders were mixed together by planetary-mill. The zeta-potential value of $TiO_2$ particles in cyclohexane was measured about -40mV, but was measured over -110mV by dispersant attached to polyethylene-coated $TiO_2$ surface. Prepared electronic ink was filled in cross patterned micro-wall with $200{\mu}m$ in width and $40{\mu}m$ in height on ITO glass designed by photolithography. The response time of electronic paper evaluated by mobility of $TiO_2$ particle between micro-walls was measured 0.067sec, but the drift velocity from reflectance wave form during reverse from of electronic ink was measured 0.07cm/sec.

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입자 영상 처리 시스템을 이용한 콜로이드 입자의 제타포텐셜 측정 및 나노유체 분산 특성 연구 (A Study on the Zeta Potential Measurement and the Stability Analysis of Nano Fluids using a Particle Image Processing System)

  • 이재근;김성찬;김희중;이창건;주찬홍;이래철
    • 한국분무공학회지
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    • 제8권1호
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    • pp.16-22
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    • 2003
  • Zeta potential measurements of colloid particles suspended in a liquid are performed by a Zeta Meter developed. There are many applications of colloid stability in spray technology, paints, wastewater treatment, and pharmaceuticalse. Zeta potentials of charged particles are obtained by measuring the electrophoretic velocities of the particles using video enhanced microscopy and image analysis program. The values of zeta potential of polystyrene latex(PSL), $silica(SiO_2)$M, polyvinylidence difluoride(PVDF), silicon nitride, and alumina particles in deionized (DI) water were measured to be -40.5, -31.9, -25.2, -15.1 and -10.1mV, respectively. The particles having high zeta potential less than -20 mV are stable in DI water, because the double layers of them have strong repulsive forces mutually, and the particles having low zeta potential over -20mV are unstable due to Van Der Waals forces. Silica(>20nm), PSL, aluminum and PVDF particles were found to be stable that would remain separate and well disperse, while silicon nitride and alumina particles were found to be unstable that would gradually agglomerate in DI water.

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