• Title/Summary/Keyword: electron microscopic analysis

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Analysis of Cutting Mechanism by Image Processing on Micro-Cutting in SEM (전자현미경내 마이크로 절삭의 화상처리에 의한 절삭 기구 해석)

  • 허성중
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.12 no.3
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    • pp.89-95
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    • 2003
  • This research analyzes the cutting mechanism of A1100-H18 of commercially pure aluminum by image processing in SEM(Scanning Electron Microscope) for the measurement of strain rate distribution near a cutting edge in orthogonal micro-cutting. The distribution is measured using various methods in order. The methods are in-situ observations of cutting process in SEM, inputting image data, a computer image processing, calculating displacements by SSDA(Sequential Similarity Detection Algorithm) and calculating strain rates by FEM. The min results obtained are as follows: (1)It enables to measure a microscopic displacement near a cutting edge. (2) An application of this system to cutting process of various materials will help to make cutting mechanism clear.

Royal Jelly Protein and Lipid Composition in Apis cerana indica F.

  • Shinkhede, Milind Manohar;Tembhare, Dnyaneshwar Bapuji
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.2
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    • pp.139-142
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    • 2009
  • The histological and transmission electron microscopic studies revealed the synthesis activity predominantly in the hypopharyngeal glands of the nurse bees. The biochemical analysis of both, the hypopharyngeal gland extract and royal jelly elucidated unequivocally the proteins and lipids as the major constituents. Further the SDS-PAGE of hypopharyngeal gland extract showed about 17 protein bands, perhaps 14.10, 20.00, 29.00 and 43.00 kDa predominantly while that of royal jelly revealed only two protein bands of 29.00 and 43.00 kDa molecular weight suggesting them as the major royal jelly proteins (MRJP). The lipid profile of royal jelly consists of triglycerides, cholesterol, HDL, LDL and VLDL.

Atomic Layer Deposition of $Sb_2S_3$ Thin Films on Mesoporous $TiO_2$

  • Han, Gyu-Seok;Jeong, Jin-Won;Seong, Myeong-Mo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.282-282
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    • 2013
  • The antimony sulfide ($Sb_2S_3$) thin films were deposited using the gas phase method which known as atomic layer deposition (ALD) on mesoporous micro-films. Tris (dimethylamido) antimony (III[$(Me_2N)_3Sb$] and hydrogensulfide ($H_2S$) were used as precursors to deposit $Sb_2S_3$. Self-terminating nature of $(Me_2N)_3Sb$ and $H_2S$ reaction were demonstrated by growth rate saturation versus precursors dosing time. Absorption spectra and extinction coefficient were investigated by UV-VIS spectroscopy. Scanning electron microscopic analysis and X-ray photoelectron spectroscopy (XPS) depth profile were employed to determine the conformal deposition.

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TEM Stud of GaN Thick Film Crystals Grown by HVPE

  • 송세안;이성국
    • Proceedings of the Korean Vacuum Society Conference
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    • 1999.07a
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    • pp.121-121
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    • 1999
  • Gallium nitride (GaN) semiconductor is intensively under investigation for commercialization of short wavelength light emitting devices and laser diodes. One of serious obstacles to overcome is to reduce the defect density in GaN film grown by various techniques such as MOCVD, HVPE, etc. Many research groups including SAIT are trying to improve the defect density to 106-107/cm2 from the level of 108-1010/cm2. We have investigated epitaxial growth behaviour of GaN thin and thick films under hidride vapour phase epitaxy (HVPE) condition. In this report, we present the microstructural and crystallographical characteristics of the GaN films grown on sapphire (0001) substrate which were studied by both conventional and high-resolution transmission electron microscopy (TEM). Also we present some microscopic analysis results obtained from GaN films grown by ELO(dpitzsial lateral overgrowth)-HVPE and from GaN quantum well structures grown by MOCVD. Another serious problem in growing GaN thick film by HVPE is internal micro-cracks. We also comment the origin of the micro-crack.

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Ultrastructural observations of vegetative cells of two new genera in the Erythropeltidales (Compsopogonophyceae, Rhodophyta): Pseudoerythrocladia and Madagascaria

  • Scott, Joseph L.;Orlova, Evguenia;West, John A.
    • ALGAE
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    • v.25 no.1
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    • pp.11-15
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    • 2010
  • Two new genera of red algae, Madagascaria erythrocladioides West et Zuccarello and Pseudoerythrocladia kornmannii West et Kikuchi (Erythropeltidales, Compsopogonophyceae, Rhodophyta), were previously described using molecular analysis and confocal microscopy of isolates in laboratory culture. We examined the ultrastructure of both genera to compare with ultrastructure of other members of the class Compsopogonophyceae. Both genera had Golgi bodies not associated with mitochondria and chloroplasts with a peripheral encircling thylakoid similar to all other members of the class studied thus far. Confocal autofluorescence images showed that Madagascaria has a single round central pyrenoid while Pseudoerythrocladia has no pyrenoid. Our electron microscopic work confirms these initial observations. Tables and keys are presented that assist in interpreting cellular details of genera in the class Compsopogonophyceae.

Characterization of Azomonas agilis PY101, a Cadmium-Resistant Strain Isolated from Anyang Stream

  • You, Kyung-Man;Lee, Ji-Hyun;Kim, Jeong-Kook;Hah, Nam-Ju;Lee, Yung-Nok;Park, Yong-Keun
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.289-293
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    • 1996
  • A cadimium-resistant strain isolated from Anyang stream, Azomonas agilis PY101 exhibited strong resistance to 1000 ppm of cadmium ion $(Cd^{2+})$. A agilis PY101 also exhibited resistance to various antibiotics, such as amoxicillin, amplicillin, bacitracin, cefazolin, erythromycin, penicillin, tetracycline, and vancomycin. In the presence of $Cd^{2+}$, the growth of A. agilis PY101 started after an extended lag phase and produced a green-fluorescent pigment induced by cadmium. The dramatic decrease (approximately 400ppm) of concentration of $cd^{2+}$ in the culture medium during the growth phase of A. agilis PY101 was confirmed by the inductively coupled plasma-atomic emission spectrophotometer. Transmission electron microscopic analysis revealed that A. agilis PY 101 actively accumulated $Cd^{2+}$ in the cytoplasm.

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First Report of Sweet potato latent virus and Sweet potato chlorotic stunt virus Isolated from Sweet Potato in Korea

  • Yun, W.S.;Lee, Y.H.;Kim, K.H.
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.126-129
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    • 2002
  • Infected sweet potato (Ipomoea batatas) showing symptoms of sunken veins, stunting, mosaic, and mottling were collected from Gimje, Cochang, Iksan, and Haenam provinces in Korea. Electron microscopic (EM) observation of the infected tissue revealed rod and filamentous rod type virus particles of various lengths. Western blot analysis of the protein samples extracted from infected sweet potato and partially purified virus identified the isolates as Sweet potato feathery motile virus (SPFMV), Sweet potato latent virus (SwPLV), and Sweet potato chlorotic stint virus (SPCSV). Sweet potatoes were occasionally infected with more than one of these viruses. This is the first report of SwPLV and SPCSV in Korea.

Phenotypes of Integrase-Mutated Human Immunodeficiency Virus Type-1(HIV-1)

  • ;Chris M. Farnet;William A. Haseltine
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1993.04a
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    • pp.92-92
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    • 1993
  • Point mutations in a highly conserved central region of the HIV-1 integrase protein were analyzed for their effects on viral replication and virion morphogenesis. Conservative amino acid replacements of two amino acid residues invariant un retroviral integrases, D116 and E152 of HIV-1, as well as the highly conserved amino acid S147, completely blocked viral replication in two CD4$\^$+/ human T cell lines. Mutation of four other highly conserved amino acids in the region had no detectable effect on viral replication, while Mutations at two positions, N117 and Y143, resulted in viruses with a delayed replication phenotype. Characteristic and reproducible defects id virion core structure were observed by electron microscopic analysis of sore of the replication defective integrase point mutants, indicating that mutant integrase proteins can interfere with the process of virion core maturation.

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Studies on Synthesis of Magnesium Carbonate. I. Factorial Analysis on the Preparation of Exrta-light Magnesium Carbonate (탄산마그네슘의 제조연구 I 경질탄산마그네슘의 제조조건)

  • Rhee, Gye-Joo;Song, Churl;Sung, Nak-Won;Ahn, Yung-Pil
    • YAKHAK HOEJI
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    • v.15 no.1
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    • pp.24-31
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    • 1971
  • Optimum reaction conditions for the preparation of extra-light magnesium carbonate from bittern by the reaction with sodium carbonate solution was found to be as follows: reaction temperature 33.deg. molar ratio(Mg$^{+2}/CO$^{2-}_{3}$)0.8, reaction time 14 minutes, drying temperature 99.deg. and bittern concentration 17%. While Korean pharmacopeia regulates the bulkiness above 12 mililiters per gm., our experimental result shows above 45 mililiters. Electron microscopic shapes were compared with products prepared under various reaction conditions, and it was found that there exists lighter the powder the more pillar crystalline, the heavier the powder the more amorphous and the intermediate was mixture of them.

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Preparation of ZnO Thin Films Using Zn/O-containing Single Precursorthrough MOCVD Method

  • Park, Jong-Pil;Kim, Sin-Kyu;Park, Jae-Young;Ok, Kang-Min;Shim, Il-Wun
    • Bulletin of the Korean Chemical Society
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    • v.30 no.1
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    • pp.114-118
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    • 2009
  • A new Zn/O single source precursor, TMEDA-Zn$(eacac)_2$, has been synthesized by using N, N, N’, N’-tetramethylethylendiamine (TMEDA), sodium ethyl-acetoacetate, and $ZnCl_2$. From this organometallic precursor, ZnO thin films have been successfully grown on Si (100) substrates through the metal organic chemical vapor deposition (MOCVD) method at relatively mild conditions in the temperature range of 390~430 ${^{\circ}C}$. The synthesized ZnO films have been found to possess average grain sizes of about 70 nm with an orientation along the c-axis. The precursor and ZnO films are characterized through infrared spectroscopy, nuclear magnetic resonance spectroscopy, EI-FAB-spectroscopy, elemental analyses, thermal analysis, X-ray diffraction, and field emission scanning electron microscopic analyses.