• Korean Journal of Animal Reproduction
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• v.21 no.4
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• pp.405-409
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• 1997

This study was carried out to investigate the general characteristics such as semen volume, pH, sperm motility and sperm concentration of the semen collected from Korean Jindo dogs by the mothod of Digital manipulation of penis, and the effect of temperature and preservation time on motility of fresh semen. Multiple ejaculates were collected from four male Korean Jindo dogs. The results obtained in this experiment were as follows : 1. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the second fraction and the small volume of third fraction from the ejaculate were 3.29ml, 6.30, 96.70% and 1.64$\times$108 cells/ml, respectively. 2. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the first fraction from the ejaculate were 1.16ml, 6.10, 6.67% and 5.07$\times$105cells/ml. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the second fraction from the ejaculate were 2.30ml, 6.33, 97.66% and 1.92$\times$108cells/ml. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the third fraction from the ejaculate were 3.24ml, 6.51, 93.33% and 3.13$\times$107cells/ml. 3. Motility of fresh semen during preservation were higher at 17$^{\circ}C$ than at 5$^{\circ}C$ or 36$^{\circ}C$. When preservation temeprature was 17$^{\circ}C$, motility were 95.75% at 1 h, 90.00% at 6 h, 84.25% at 12 h, 68.00% at 18 h, 36.25% at 24 h and 28.75% at 30 h, respectively.

• Korean Journal of Animal Reproduction
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• v.5 no.2
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• pp.35-42
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• 1981

A total 2,488 ejaculates during 4 years from 80 Korean native bulls over the age from 3 to 12 years of bull herds of Artificial Breeding Center, National Livestock Federatives Cooperation were collected and analyzed to study the effects of collection year, age of bulls, month of years, collection interval and ejaculation frequency per day on semen volume, sperm concentration, total sperm per ejaculate and sperm motility. 1. Semen volume, sperm concentration, total sperm per ejaculate and sperm motility index for the all ejaculates using least squares procedure averaged 5.73ml, 9.133${\times}$108/ml, 52.527${\times}$108 and 62.81, respectively. 2. Semen volume varied significantly with collection year, collection interval and ejaculation frequency per day(P<0.01), but effects of age of bulls and month of years on semen volume were not significant. Eight to 15 days collection interval showed the highest volume, and 1st ejaculate contained 5.6% more volume than 2nd ejaculate. 3. There were significant differences among collection years, months of years and ejeculation frequencies per day except collection intervals in sperm concentration per ml(P<0.05, P<0.01). Six to 8-year-old bulls was the highest concentration. Higher sperm concentration per ml was in April to July and lower month was October and December. Sperm concentration in 2nd ejaculate was higher than in 1st ejaculate. 4. Total sperm per ejaculate affected by all environmental factors studied(P<0.05, P<0.01). Age of bulls, collection interval and ejaculation frequency per day showed the highest total sperm was 6 to 8-year-old bulls, 8 to 15 days interval and 1st ejaculate, respectively. Higher total sperm per ejaculate was in April to July and lower total sperm was in September to December. 5. In sperm motility, there were significant differences among collection years, ages of bulls and collection intervals except months of years and ejaculation frequencies (P<0.01). Higher sperm motility was in 6 to 12-year-old bulls and in 5 to 7 days collection interval.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.4
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• pp.445-449
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• 1998

Semen quality was compared between the local Katjang and the cross-bred (local Katjang ♀ ${\times}$ German Fawn ♂) bucks. There were on significant genotypic differences in semen characteristics of concentration (first ejaculate : $6.19{\pm}1.30$ -versus $6.33{\pm}1.40{\times}10^9/ml;$second ejaculate: $5.82{\pm}1.10$ - versus $5.68{\pm}1.45{\times}10^9/ml$, for Katjang and the cross-breds, respectively), percentage live (first ejaculate: $77.61{\pm}1.33%$ versus $77.81{\pm}0.53%$; second ejaculate: $81.97{\pm}1.59%$ versus $82.74{\pm}0.96%$, for Katjang and cross-breds, respectively) and percentage of normal sperms (first ejaculate: $12.54{\pm}3.88%$ versus $26.45{\pm}3.83%$; second ejaculate: $38.68{\pm}3.65%$ versus $28.54{\pm}4.38%$, for Katjang and cross-breds, respectively), with the exception of seminal volume and sperm motility. Means of all variables were within the values reported for other goat breeds, In contrast, the differences in semen characteristics between the first and second ejaculations of both genotypes were more distinct, the second ejaculations always had more volume, more normal sperms and better sperm motility but less sperm concentrations. Removing the seminal plasma and replacing it with tris-citrate buffer greatly prolonged the viability of sperms of both genotypes when stored at $5{^{\circ}C}$. Sperm motility seens to be a good indicator of sperm viability. However, the sperms of the corss-bred bucks withstood the washing process better and their swimming abilities were superior ($8.12{\pm}0.46mm/min$) when compared to those of the local Katjang breed ($5.42{\pm}0.49mm/min$). The higher content of calcium ions in their seminal plasma (first ejaculate: $10.5{\pm}0.8$ versus $10.6{\pm}0.8mg/100ml$;second ejaculate: $15.3{\pm}0.8$ versus $16.1{\pm}0.8mg/100ml$, for Katjang and cross-breds, respectively) means that in natural matings the sperms of the cross-breds would be at an advantage compared to those of the local Katjang, since calcium ions reportedly initiate acrosomal reactions.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.439-447
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• 1992

Eight healthy Holstein bulls, 4-6 years old were used to study the effect of season of the year on the biophysical characteristics of semen. Semen was collected twice a week by AV (artificial vagina) over one-year period. The analyses revealed that all the basic seminal traits studied were differed significantly due to season, except the ejaculate volume and consistency and the percentage of swollen spermatozoa in a hypo-osmotic fructose-citrate solution. Ejaculates collected during hot summer season had significantly lower sperm motility, concentration and total counts, and higher percentage of dead spermatozoa than those collected during winter time. Warm spring had moderate semen quality. The temperature-humidity index was calculated and it was associated (p < 0.01) negatively with the ejaculate pH, sperm concentration and total counts, and positively with the % of dead sperms. Ejaculate volume, percentage of swollen spermatozoa, individual motilities did not correlate significantly with the change in temperature-humidity index values. The total live, motile spermatozoa per ejaculate during both the winter and spring seasons showed significant increase of about 37% and 32% respectively over the summer season. Also, rectal temperatures of the bulls were elevated during the hot summer season, while the values of blood hemoglobin and packed-cell volume were decreased.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.2
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• pp.51-57
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• 2011

Reproductive activity in the economically important insect, Antheraea mylitta is very important because the female reproduces only once in her life time and the aging is very rapid and costly. It is a capital breeder and strictly a monoandrous species. To know how strategically the insect behaves and the male allocates its ejaculate resource more prudently during its successive mating with virgin females to maximize its own fitness and the fitness of the female with whom it mated, both being most important to sericulture industry. So, the present study was undertaken and the results revealed fresh and virgin females always prefer to mate with fresh virgin males (84%) and receives high dose of ejaculates leading to higher hatchability than to virgin males of one day old (13.7%) and one day old males with mating experience (2.3%). The ejaculation size (as referred to eupyrene sperm count in the ejaculation) declined significantly over successive mating (r = -0.9931, P < 0.001), so also the male body weight (r = -0.9560, P < 0.001). The quantity of ejaculate passed to female also dramatically declined during aging (r = -0.9982, P < 0.001). It was found that male weight contribute substantially to the quantum of ejaculate (r = -0.9519, P < 0.001), so also higher fecund females receive relatively more ejaculate than the lower group to reach higher reproductive fitness. The life time fecundity was found to be $334{\pm}31$.

• BMB Reports
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• v.48 no.5
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• pp.241-242
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• 2015

Reproductive behaviors have evolved through severe inter-sexual competition. We have recently described a behavior in post-mated female Drosophila melanogaster that controls ejaculate retention and sperm storage, and is a possible mechanism by which females who have mated with several partners can choose which sperm that is stored and used for fertilization. This behavior can also regulate exposure of the female to harmful effects of male SFP that are present in the ejaculate. Our study identified the neural pathway functioning in the female brain that regulates this behavior. [BMB Reports 2015; 48(5): 241-242]

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.359-364
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• 2007

The objective of this study was to evaluate monthly variation in semen characteristics in Black Bedouin and Black Bedouin${\times}$Damascus crossbred bucks. Twenty one Black Bedouin Goat bucks (BG) and 20 $F_1$ Black Bedouin${\times}$Damascus crossbred goat bucks (CB) were used in the study. Animals were 2 to 5 years old at the beginning of the study. Body weight (BW), body condition score (BCS), scrotal circumference (SC) and semen samples were evaluated monthly for all bucks. Body weight, BCS and SC differed according to month of collection (p<0.0001). Body weight and BCS were greater in the spring and summer months while SC reached their highest values during the autumn months. No differences were detected between breeds with respect to semen characteristics with the exception of ejaculate volume (p<0.05) and ejaculate appearance (p=0.053) which were greater in CB than in BG bucks. With the exception of ejaculate appearance, all semen characteristics were influenced by month of collection (p<0.05). Semen concentration and motility were better during the late summer and autumn months than during the spring. The percentage of abnormal spermatozoa declined from its greatest values in the spring to its lowest values in the autumn. Results of the present study show no effects of goat breed on most of the studied parameters. In general, most semen quality parameters were better in both breeds during the autumn than the spring months. Minimal variation in semen quality was observed between the summer and autumn months indicating the ability to use bucks for semen collection and for natural breeding during both of these seasons.

• Korean Journal of Animal Reproduction
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• v.9 no.1
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• pp.40-45
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• 1985

Eight 2-yr old bulls from Artificial Breeding Center, NLCF were used to determine the effectof collection frequency on semen characteristics and sexual activity. Two successive ejaculates per day were collected by artificial vagina for 4 weeks on weekly or twice a week. Total ejaculate volume included 2nd ejaculates for one time and two time bulls was 6.8ml and 6.0ml, but there was no significant difference between collection intervals. Sperm concentration of one time and two time bulls averaged 0.79$\times$109/ml and 0.89$\times$109/ml, respectively. Total sperm per ejaculate was 5.14$\times$109 for one time bulls and 5.45$\times$10 for two time bulls. Two time bulls had slight more sperm per ml and ejaculate than one time bulls, but there were no significant differences between two group bulls. Sperm motility and semen pH of two time bulls was slightly better than that of one time bulls. In changes of bulk minerals in semen, solium concentration of two time bulls was similar to that of one time bulls. Potassium and calcium was more concentrated in one time bulls than in two time bulls, but these concentrations did not differ significantly. Libido score for two time bulls was higher than that for one time bulls. However, there was no difference between two groups and these scores did not change for 4 weeks in two goups. Total time to 2nd ejaculation was 16.3 sec for one time bulls and 20.5 sec for two time bulls.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.2
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• pp.184-188
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• 2001

In a summer study during May to July, involving 12 young Murrah buffalo bulls at forty months of average age, the effects of multiple shower vs single shower body cooling and vitamin A, D and E supplementation on the sexual behaviour, semen quality and freezability were investigated. The animals were divided into two groups (6 animals in each group) and housed in a half-walled shed with proper spacing, the feeding management being identical. The bulls in the control group were given a single shower at 1000 h, whereas the experimental bulls were given four showers at 10,12,14 and 16 h. In addition, the experimental bulls were given vitamin A, D and E injections at fifteen day intervals. The sexual behaviour of bulls was observed in terms of reaction time, sexual aggressiveness and ejaculatory thrust. Semen quality of all the bulls was assessed in terms of volume, mass activity, live-dead sperm and sperm concentration, sperm motility and morphology, and acrosomal abnormality. The sexual behaviour did not vary significantly between the groups, whereas semen quality differed significantly for volume, per cent live sperms, total sperms per ejaculate and total live sperm per ejaculate between groups. It can be concluded that sexual behaviour was not influenced by the thermal comfort treatment coupled with periodic vitamin A, D and E injections. But the treatments improved most of the seminal traits in the experimental group of bulls. However, benefit of treatment was not reflected in the freezability traits of the semen.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.2
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• pp.91-98
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• 2021

The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 10⁶ cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 10⁶ cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.