Monthly changes of the gonad follicle index (GFI), reproductive cycle, egg-diameter composition, first sexual maturity of the Pacific oyster, Crassostrea gigas, were studied based on the samples which have been collected from the intertidal zone of Poryong west coast of Korea, from January to December, 1996. C. gigas, is dioecious, while a few individuals are alternatively hermaphroditic. Monthly variation of gonad follicle index (GFI) used for determination of spawning period, coincided with the reproductive cycle. GFI increased from April when seawater temperatures gradually increased and reached the maximum in May. And then, GFI sharply decreased from June to September due to spawning. Reproductive cycle of this species can be divided into five successive stages: in females, early active stage (March to April), late active stage (April to May), ripe stage (May to August), partially spawned stage (June to September) and spent/inactive stage (September to February); in males, early active stage (February to March), late active stage (April to May), ripe stage (May to September), partially spawned stage (June to September) and spent/ inactive stage (September to February). The diameter of fully mature eggs are approximately 50um. Spawning occurred from June to September, and two spawning peaks were observed in June and August when the seawater temperature was above $20^{\circ}C$. Percentages of the first sexual maturity of males of 20.1-25.0 mm in shell height were over $50\%$, while those of females of 25.1-30.0 mm in shell height were over $50\%$. All the males of > 30.1 mm and all the females of ^gt; 35.1 mm completed their first sexual maturity. The results suggest that C. gigas has a protandry phenomenon. Sex ratios of 919 oysters observed were 453 females $(49.29\%)$, 429 males $(46.68\%)$, 16 hermaphrodites $(1.74\%)$, and 21 indeterminate individuals $(2.29\%)$. In age class I, sex ratio of males were $64.00\%$, thus, a higher percentage than that of females. It was noted that $64.00\%$ of the young males (age class I) were more functional than females in age class I, but 2-3 year-old oysters showed higher percentage of females. Percentages of hemaphrodites in 2-3 year classes were relatively higher than those in other year classes. Histological pattern of hermaphrodites can be divided into two types: Type I (hermaphrodite having a number of newly formed developing oocytes on the oogenic tissues within a degenerating spermatogenic follicle after discharge of numerous spermatozoa) and Type II (hermaphrodite having two separate follicles in the same gonad).
A study was conducted to investigate the relationships between the rice weevil and associated storage molds. The results are as follows; 1. All of the developmental stages of the rice weevils are carrying some storage molds in their bodies, and the order of magnitute in the number was the adult, larva and pupa. 2. The molds persist in the body of the rice weevil for 10 days who.1 they were fed on the mold free wheat, and the most persistant mold species was A. candidus. 3. When the mold free weevils were reared on the pure culture of the molds on the wheat, the number of eggs laid by the Lveevil were the greatest for A. candidus. following A. ruber, and the . least number Lvere obtained with A. niger 4. The rice weevil could complete in the pure mold culture on the wheat except for A. niger where the larvae had developed by 2nd or 3rd instars. 3. The shortest developmental periods was obtained with A. candidus and the first adult emerged in 4th week. 6. The unfavorable effects of A. niger on the development of the rice weevil might be associated with the fast growth of the mold together with some unknown effects. 7. There seems to be a protocooperative interaction between these two oraganisms having been developed through the long evolutionary course in common habitat.
A study was carried out to test sex ratio of the progeny that were induced by the artificial fertilization between normal female and gynogenetic diploid eggs and sex-reversed gynogenetic diploid male sperm. Sex reversed gynogenetic diploid males were produced successfully in gynogenetic diploid female by immersion treatment with $17{\alpha}$-methyltestosterone at the concentration of 10 pub from $13.5{\pm}0.22$ mm to $57.6{\pm}5.1$ mm in total length. In $F_1$ progeny test, matings between normal females and sex-reversed gynogenetic males were not produced all female, but matings between gynogenetic diploid females and sex-reversed gynogenetic males were produced all female populations in six out of eight groups and two lots produced $96.9\%$ and $95\%$ female. These data showed that all-female populations were produced by crossing between gynogenetic diploid female and sex reversed gynogenetic diploid male.
For development of all male seed production at hatchery scale, a comparative study was made on the seed production traits of supermale (IT - t) and superfemale (IT - L1 ~) Nile tilapia, Oreochromis niloticus. Supermales were crossed with normal females (XX- f) and the superfemales with normal males (XY - t ) or sex reversed males (XX- L1 t); progeny survival of these crossings and sex ratio were evaluated. Hatching success of the eggs, fertilized by the supermale was significantly lower than that by the normal male. Over 95-99 % progenies sired by crossing supermales with normal females were males, while 52-55 % progenies alone were males with the cross of normal males and normal females. Hatching success and survival of alevins were significantly higher for the progenies of the crosses between superfemales and sex reversed males than those resulting from the crosses between superfemales and normal males. However, there was no significant difference in the sex ratio among the progenies of these crosses. Therefore, crossings of superfemales with sex reversed males provide the highest percentage of survival and male progenies.
Morphological characteristics, developmental period, and seasonal occurrence of Adeia leucomelas (L.) were investigated from 1999 to 2000. In addition, consumption of sweetpotato as food was also examined. Adults of A. leucomelas were dark-brown and body lengths of females and males were 20.2 mm and 18.9 mm, respectively, Wing expanse of female and male was 33.7 mm and 29.4mm, respectively. Egg was flat round-shape. Larva was light yellow-green to dark-brown with 3.3-53.5 mm. Pupa was deep-brown and 15.1 mm in length. Developmental periods of A. leucomelas from egg to adult emergence at different temperatures of 15, 20, 25, and 30$^{\circ}$C were 108.5, 70.7, 40.2, and 29.1 days, respectively, Developmental threshold (DT) and effective accumulative temperatures were estimated as 10.7$^{\circ}$C and 67.5 DD in egg stage, 11.0$^{\circ}$C and 275.1 DD in larval stage and 9.3$^{\circ}$C and 244.6 DD in pupal stage, respectively. The longevity of adult female was shortened with increment of temperature, whereas the total numbers of eggs laid by a female were increased. The larvae of A.leucomelas occurred from mid-June to early October, and population reached its peak during early to mid-September in Jeonbuk province. Food consumption of A. leucomelas was highest at 20-25$^{\circ}$C. Food consumption of 1 st, 2nd, 3rd, 4th, and 5th larvae of A. leucomelas per day at 25$^{\circ}$C was 0.4, 3.6, 19.6, 40.7, and 78.9 $\textrm{cm}^2$, respectively.
The embryonic and postembryonic developments of Nezara antennata Scott were observed in 5 different rearing cages such as A (Cylindrical, ${\phi}\;10cm{\times}4cm$), B (Cylindrical, ${\phi}\;14.5cm{\times}2.8cm$), C (Rectangle, $6.5L{\times}6.5cmW{\times}10cmH$), D (Cylindrical, ${\phi}$ 9cm in bottom & ${\phi}$ 11.5 cm in $upper{\times}10.8cm$) and E (Cylindrical, ${\phi}\;15cm{\times}7.5cm$) containing soybean and peanut seeds as food, and sponge soaked with water under laboratory condition of $24^{\circ}C$ and 15L : 9D. Hatchability ranged from 93 to 97%. Nymphal duration was shortest of 6 days in the 1st instar and longest of 10 days in the 5th instar. The nymphal duration was 38 to 39 days observed in the rearing cages. Emergence rate was in the range from 53 to 62% with highest in A and B cages. Adult longevity was 65 to 75 days for male, and 67 to 74 days for female, and was longest in the B cage. Total number of eggs laid by female adult was in the range from 51 to 56 without significant difference in the rearing cages, and was the most in the B cage. Accordingly, the reproductive rate of N. antennata for 1 generation was within 25 to 33 times, and was highest in the B rearing cage. Therefore, it could be concluded that B cage is most suitable for stable rearing of N. antennata under laboratory condition.
Kareius bicoloratus (Basilewsky) is one of the commonly found right-eye flounders and widely distributed in the coastal waters of Korea and Japan. On December 11,1980, the ailthors carried out an experiment to obtain a large number of fertilized eggs from wild adult fish caught by a trawler. The fish were obtained from Maisaka fish market, Shizuoka Prefecture, Japan. The egg is pelagic, spherical in shape and measuring 1.014-1.04 mm in diameter. The yolk as well as the egg capsule is colorless and transparent, and contain no oil globules. The hatching took place in 73 hr 45 min after fertilization at the water temperature $8.0-9.5^{\circ}C$. Newly hatched larvae are 3.09-3.146 mm in total length, with the anus situated in the middle of the body. The marginal fin does not have Pigment cells and myotome number is 17+20=37. Within one day after hatching, the larvae attained 3.77 mm in total length, and there appeared three or four melanophore on yolk sac. When the larvae attained 3.96 mm in total length, melanophores began to appear on the eye ball. Two days after hatching, the larvae attained 4.05 mm in total length, most of yolk material was absorbed, and the caudal fin began to grow at the terminal part of the notochord. When the larvae attained 4.21 mm in total length, mouth and eyes began to move. After 3 days, the larvae attained 4.342-4.394 mm in total length, alimentary canal differentiated, melanophores appeared on the lower jaw and posterior part of the fin membrane. When the larvae attaind 4.576 mm in total length, marginal line of dorsal fin membrane became concave.
This study was conducted to understand the settlement process of Ascotis selenaria larvae into citrus orchards with respect to oviposition site and analysis of the spatial distribution pattern of the larvae. A. selenaria eggs were not found on citrus trees in field and green house, but not on citrus trees in the field. A. selenaria larvae showed a significant clump distribution in the greenhouse. In the open citrus field, the index of dispersion was around 1.0 in most cases, with a weak clumping degree. However, the d-statistic was between -1.96 and 1.96, indicating a statistically significant random distribution. In addition, the Green's index (a clumping index) was very low in all cases, even though the clump distribution was accepted. for most samples, the probability distribution of larval frequency in the field satisfied the probability distribution functions of Poisson (random pattern) and the negative binomial (clump pattern) distribution. In addition, the temporal distribution of the larvae in the open field showed a pattern which was formed by colonizers from outside oviposition sites. Further, the difference in larval spatial distribution between field and greenhouse orchards was discussed.
Journal of the Korea Academia-Industrial cooperation Society
/
v.21
no.3
/
pp.173-179
/
2020
The purpose of this study was to examine whether chicken-rearing activity is effective on the character, emotion and self-esteem of middle school students, as a pilot study for the development of an animal-assisted education program based on chicken-rearing activity. School was selected as the place where students have experience with raising animals before and no poultry farms were located near the school. The experimental group consisted of 11 students as the chicken-rearing group. The conducted activities were greeting chickens, cleaning henhouse, feeding them, and picking eggs up for five months. Character, emotion and self-esteem scales were used as test tools, and pre-testing and post-testing were conducted to verify the effectiveness of the study. Data collected were analyzed by using SPSS Win 25.0 statistical program. As a result, the character, emotional stability and self-esteem of middle school students who participated in chicken-rearing activity were improved. This study is meaningful in that the chicken-rearing activity proved to be effective for the character, emotion and self-esteem of middle school students and prepared an experimental case study for developing an animal-assisted education program based on chicken-rearing activity. It is expected to be useful as basic data of animal-assisted education for adolescents in school.
For ecological study of Synechogobius hasta the environmental factors, length composition, stages of ovarian maturation, growth, and stomach contents of this species were examined. The samples were collected from the Kum River estuary from May, 1994 to June, 1995. The ovarian egg development of this species underwent 4 stages : the oogonium stage in October to November, growth stage in December to February of next year, maturity stage in February to April, and spawning stage in late April to middle May. The peak spawning period appeared in early to middle May. The fecundity varied from 8,600 to 49,000 showing a exponential increase by body size. The minimum size having matured eggs was 225 mm in total length (standard length 180 mm). The larvae smaller than 10 mm appeared in late May, and young fish of total length $13\~15mm$ entered into bottom life in the shallow waters. The young fish grew rapidly and reached about 141.7 mm in late October. The fish inhabited in the subtidal zone from December to May of next year when began to spawn. The largest specimen examined in this study was 531 mm of male, 472 mm of female. The major food items of young fishes were copepods and invertebrate larvae, and those of adult fishes were crabs, fish, shrimps, cephalopods, and polychaetes.
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