• Title/Summary/Keyword: egg counts

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Studies on the Fermentation of Egg by Lactic Acid Bacteria -I. Change of Lactic Acid Bacterial Cell Counts, Titrable Acidity and pH in Fermented Egg- (유산균(乳酸菌)에 의(依)한 란(卵)의 발효(醱酵)에 관(關)한 연구(硏究) -제(第) 1 보(報) : 발효란중(醱酵卵中)의 유산균수(乳酸菌數), 적정산도(滴定酸度) 및 pH 변화(變化)-)

  • Kim, Chang-Han;Ha, Jung-Uk;Kim, Si-Goan
    • Korean Journal of Food Science and Technology
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    • v.15 no.2
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    • pp.118-122
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    • 1983
  • The whole eggs with of without added 1% of glucose, lactose and sucrose respectively were pasteurized at $58^{\circ}C$ for 30min and then incubated for 24 hrs after inoculating with S. lactis, L. casei and S. faecalis to investigate the changes in lactic acid bacterial cell counts, titable acidity and pH. Fresh eggs were not contaminated with common bacteria and Salmonella, Proliferation of L. casei was the best among those microorganisms in pasteurized whole egg without sugar added, and titrable acidity was the highst and pH the lowest in the eggs fermented with L. casei. However, lactic acid bacterial cell counts, titrable acidity and pH were changed significantly by the addition of sugar, especially the growth of S. faecalis was better than two other organisms, and lactic acid bacterial cell counts, titrable acidity and pH of the eggs fermented with S. faecalis were $2.5{\times}10^{10}$, 0.70 and 4.8 respectively after 24 hrs fermentation.

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Epidemiologic Investigation on an Outbreak of Salmonellosis in Yeongcheon-si, 2004 (2004년 영천시 D 초등학교에서 집단 발생한 살모넬라증)

  • Min, Young-Sun;Lee, Hwan-Seok;Lim, Hyun-Sul
    • Journal of Preventive Medicine and Public Health
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    • v.38 no.4
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    • pp.457-464
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    • 2005
  • Objectives : An outbreak of salmonellosis occurred among the student s and staff of D primary school in Yeongcheon-si, 2004. This investigation was carried out to prevent any recurrence of this outbreak and to study the infection source and transmission of the salmonellosis. Methods : The authors conducted a questionnaire survey among 1,205 students and staff members from D primary school about the ingestion of the school lunch and drinking water, and the manifestation of their symptoms. The author examined rectal swabs, the tap water and microorganism cultures, and we also investigated the dining facility and water supply facility. Results : The diarrheal cases were defined as the confirmed cases or the persons who had diarrhea more than one time with symptoms such as fever, vomiting and tenesmus. The diarrheal attack rate was 28.0%. Ingestion of fried bean curd with egg had a significantly high association with the diarrheal attack rate (p<0.05), and the relative risk of the fried bean curd with egg was 10.68 (95% CI=3.88-29.41), as was determined by logistic regression analysis. The bacterial counts in the tap water of the food preparation room and toilet (first floor) exceeded the maximum permissible counts. S. Enteritidis bacteria were only cultured from the fried bean curd with egg of all the supplied foods on September 3, 2004. Conclusions : The major cause of salmonellosis was presumed to be the contaminated bean curd via contaminated tap water due to a water leak of a school water pipe. This contaminated bean curd was under prepared, which allowed the S. Enteritidis to survive and multiply prior to its ingestion.

Effects of Experimental Haemonchus contortus Infection on Red Blood Cells and White Blood Cells of Growing Goats

  • Howlader, M.M.R.;Capitan, S.S.;Eduardo, S.L.;Roxas, N.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.6
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    • pp.679-682
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    • 1997
  • A uniform group of 12 upgraded growing goats aged between 6.0 and 7.5 months were used in this study. They were divided into three groups of $T_1$, $T_2$ and $T_3$. Four animals were randomly allocated to each group. They were infected orally with three levels (0 larva, 5,000 larvae and 10,000 larvae) of infective Haemonchus contortus larvae. Before infection, all animals were housed in individual pens with concrete floors. They were provided with a uniform management. Total red blood cells (RBC) and total white blood cells (WBC) were measured by hemacytometric method. Results showed significant interaction effect of H. contortus infection and duration of infection on red blood cell counts. The RBC counts of animals in treatment groups 2 and 3 showed significantly lower values over the control group from the second fortnight to the end of the study. The overall mean RBC values of groups 1, 2 and 3 were 11.73, 9.70 and $9.12million/mm^3$ blood, respectively. H. contortus infection did not significantly influence the total leukocyte counts. Worm infection and duration of infection interaction was also absent on WBC counts. However, the time or duration of infection significantly influenced the WBC counts. Fecal egg counts showed patent infections in the infected animals which also indicated by postmortem worm counts.

Determination of the quality of stripe-marked and cracked eggs during storage

  • Liu, Yu Chi;Chen, Ter Hsin;Wu, Ying Chen;Tan, Fa Jui
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.7
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    • pp.1013-1020
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    • 2017
  • Objective: Stripe marks, which occasionally occur on the shell, do not cause breakage to the shell and shell membranes of eggs. This study investigated the quality of intact eggs (IEs), minor stripe-marked eggs (MEs), severe stripe-marked eggs (SEs), and cracked eggs (CEs) during 3-week storage at $25^{\circ}C$. Methods: Shell eggs were collected the day after being laid and were washed. Among them, eggs without any visual cracks or stripe marks on the shells were evaluated as IEs by the plant employees using candling in a darkened egg storage room; the remaining eggs exhibited some eggshell defects. At day 3, the eggs were further categorized into IEs, MEs, SEs, CEs, and broken eggs (BEs) on the basis of the description given. Except BEs, which were discarded, the remaining eggs were stored at $25^{\circ}C$ (approximate relative humidity 50%) and then analyzed. Results: Stripe marks were observed primarily within the first 3 days after washing. At day 3, CEs had significantly (p<0.05) lower Haugh unit values, but all eggs had grades AA or A, according to the United States Department of Agriculture standard. As storage time increased, differences in egg quality between groups were more obvious. IEs had the highest eggshell breaking strength. During storage, the total plate counts and pathogens, namely Escherichia coli, Campylobacter spp., Staphylococcus aureus, and Salmonella spp., were not detectable in the internal content of IEs and SEs. Conclusion: In conclusion, cracks degraded egg quality severely and minor stripe marks only slightly influenced the egg quality.

Peri-Parturient Nematode Egg Rise in Indonesian Ewes

  • Batubara, A.;Dorny, P.;Pandey, VS.;Romjali, E.;Feldman, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.3
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    • pp.293-297
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    • 1997
  • Studies were designed to study the peri-parturient rise in strongyle faecal egg counts (FEC) in ewes grazing in a rubber plantation in North Sumatra, Indonesia. In three consecutive lambing seasons strongyle FEC of peri-parturient ewes and dry ewes were monitored from 2 to 3 weeks before lambing until 7 to 9 weeks post lambing to measure the magnitude and duration of the peri-parturient rise. A significant rise in FEC was observed during the post-parturient period in two of the three studies. FEC increased in the three studies from lambing day, and were elevated until 5 to 9 weeks following lambing. Lactating ewes shed on average 3 times more eggs than dry ewes. Lactating ewes were therefore considered as an important source of pasture infection. These findings are discussed in relation to the epidemiology and control of gastrointestinal nematodes in the humid tropics.

Assessment of Baried-Parker Agar as Screening Test for Determination of Staphylococcus aureus in Poultry Meat

  • Rosa Capita;Calleja, Carlos-Alnoso;Benito Moreno
    • Journal of Microbiology
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    • v.39 no.4
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    • pp.321-325
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    • 2001
  • Baird-Parker agar with egg yolk/tellurite emulsion (BPA) is widely accepted as a medium for the enu-meration of Staphylococcus aureus in foods. Howerver, it is not vompletely selective and colonies of other genera of species could be similar to those of Staphylococcus aureus. Moreover, the strains of Staphylococcus aureus that are lecithinase negatrive could go unnoticed. Both facts could affect the counts. The aim of this study was to dtermine whether the enumeration of the colonies with the typical morphol-ogy of Staphylococcus aureus on BPA is sufficien to quantity this species in poultry meat. Forty chiken carcasses were tested for Staphylococcus aureus by surface plating using BPA, Results indicate that the predictiv value of the morphoogy of the colonies of BPA is 85.71% and 68.42% for typical and atypical colonies of Staphylococcus aureus, respectively. However, Staphylococcus aureus counts (after identification) and counts of typical colonies did not show any significant differences(P>0.05) and are significantly(P<0.001) correlated (r=0.996).These results suggest that , for screening purposes. enumeration of Staphylococcus aureus from poultry meat does not require any identification of stains. resulting in a saving of time and money.

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Quality Changes of Egg Products during Storage (계란가공품의 보관중 품질 변화)

  • Kim, Jae-Wook;Kim, Hyong-Chan;Hur, Jong-Wha
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1480-1483
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    • 1998
  • To know the probable storage conditions for commercially prepared ‘baked egg’ and ‘rolled egg’ products, microbes and quality changes for samples immediately after the production and stored under the several conditions were tested. Sealed samples were stored at 5, 10, 15, $20^{\circ}C$, respectively and tested at 1 or 2-days intervals, opened samples were stored at 15, 20, 30, $37^{\circ}C$, respectively and tested at 6-hr intervals. Coliform bacteria, E. coli, Salmonella, Staphylococci were not detected, and viable cell counts level was under $10^2\;CFU/g$ for samples immediately after the production. The higher the storage temperature and baked eggs rether than rolled eggs showed more rapid microbial growth for both samples. For sealed condition, 14-days at $5^{\circ}C$ for both samples, 7-days for rolled eggs and 5-days for baked eggs at $10^{\circ}C$ were kept their initial microbial level and favorable flavor. For opened condition, it showed remarkable microbial increase after $18{\sim}24\;hr$ for $15{\sim}20^{\circ}C$ and 12 hr for $30{\sim}37^{\circ}C$. From these results, used egg products samples are able to be stored more than 1-week at below $5^{\circ}C$ and opened samples need to be stored at cold storage. At room temperature, it egg products.

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Effects of Stomach Worm (Haemonchus contortus) Infection on The Kids Born of Infected Mother Goats

  • Howlader, M.M.R.;Capitan, S.S.;Eduardo, S.L.;Roxas, N.P.;Sevilla, C.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.4
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    • pp.435-438
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    • 1997
  • Twelve newborn goat kids born of philippine does (3.5 and 5 years old) were used in this study. Four mother (candidate) doe goats were randomly allocated to each group. The mother does were infected orally with three levels (0, 15,000 and 30,000 larvae) of infective Haemonchus contortus larvae. Before infection all animals were housed in individual pens with concrete floors. They were provided with a uniform management. Estrus of does were synchronized using PGF2 alpha. All the animals were bred naturally by the same buck. Baby goat born of infected mother goats were divided into three groups. $T_1$, $T_2$ and $T_3$), respectively, from mother treatment groups. Birth weight and growth of goat kids born from H. contortus infected mother goats were determined. Birth weights of kids of $T_1$, $T_2$ and $T_3$ were 1.9, 1.5 and 1.2 kg, respectively. No significant (p > 0.05) differences in birth weight of kids for the 3 treatment groups were found. However, significant (p < 0.05) effects of stomach worm infection and duration of infection on liveweight gain of kids were observed. After second and third week of birth, respectively, the kids of groups 3 and 2 registered lover liveweight gains than the kids in control group. However, no significant (p > 0.05) difference in liveweight gain was found between the kids of infected mother does. Fecal egg counts of the infected mother does showed patent infections which also indicated by postmortem worm counts. However, no worm egg was found in the feces of the test kids.

Changes in the Microbial Qualities and Sensory Characteristics of Boiled Quail Egg and Fish Paste in Soy Sauce as Prepared with the Cook-Chill System and Sous Vide Cook-Chill System (Cook-Chill System과 Sous vide Cook-Chill System으로 생산된 메추리알 어묵조림의 저장기간에 따른 미생물적 품질 및 관능특성의 변화(2))

  • Song, Sun-Mi;Kim, Heh-Young;Ko, Sung-Hee
    • Korean journal of food and cookery science
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    • v.23 no.2 s.98
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    • pp.261-269
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    • 2007
  • This study was performed to provide basic data for the sous vide cook-chill(SVCC) System by comparing and evaluating food quality, according to preparation method and days of storage, of foods that were prepared by SVCC to those prepared using the cook-chill(CC) System. Boiled quail eggs and fish paste in soy sauce were prepared using CC and SVCC and their quality was evaluated at the time of preparation and by days of storage. Viable cell counts were increased to 5.65(CC) and 3.40(SVCC) LogCFU/g by 15 days(p<.0001) and increased more over time in foods prepared with CC than with SVCC. For the CC method, Coliform counts increased to 4.58 LogCFU/g by 15 days(p<.0001). With SVCC, colirorms were not detected at 0 days, but counts reached 3.70 LogCFU/g by 15 days(p<.0001). After reheating, no coliforms were detected for CC or SVCC at 0 days, however, by 15 days, coliform counts reached 3.61 and 2.52 LogCFU/g(p<.0001) for CC and SVCC, respectively. Finally, the sensory scores of SVCC were higher than those of CC.

Investigation for freshness and nutritive components of the eggs sold in Incheon (인천지역 유통 계란의 신선도 및 영양성분 함량 조사)

  • Hong, Seong-Hee;Ra, Do-Kyung;Yun, Ga-Ri;Joung, Yun-Joung;Nam, Ji-Hyeon;Cheong, Eun-Ha;Lee, Jung-Goo;Lee, Sung-Mo
    • Korean Journal of Veterinary Service
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    • v.35 no.2
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    • pp.119-128
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    • 2012
  • This study was performed to investigate the freshness and nutritive components of eggs from Incheon area. The eggs of 64 brands collected from 7 hypermarkets and 6 brands collected from farms were examined to test the Haugh unit (HU), egg yolk index and specific gravity. HU and specific gravity were higher in farm eggs than in hypermarket eggs (P<0.05), and there was no difference in the egg yolk index. Among hypermarket eggs, HU and egg yolk index were higher in the graded eggs than in non-graded eggs (P<0.05), and specific gravity was not different between them. HU and egg yolk index of the eggs collected in March were higher than those in August (P<0.05). To monitor the bacterial contamination, total coliforms, total bacterial counts (TBC) and Salmonella enteritidis were examined. Salmonella enteritidis and total coliforms were not detected from all eggs. TBC of farm eggs were less than $10^1$ CFU/mL in all samples and TBC of hypermarket eggs were less than $10^1$ CFU/mL in 187 samples, $10^1{\sim}10^2$ CFU/mL in 3 samples and $10^3{\sim}10^4$ CFU/mL in 2 samples, respectively. Antimicrobial residues were not detected from all eggs tested. The vitamin E contents in the vitamin E enriched eggs from 6 brands hypermarket were 1.98~22.96 mg/yolk100 g ($14.04{\pm}8.81$ mg/yolk100g), and those of 5 brands among them were higher than the average of ordinary eggs. In one brand egg, vitamin E content was lower than the average of ordinary eggs. The vitamin E contents of ordinary eggs were 1.75~16.36 mg/yolk100 g (average $5.57{\pm}2.76$ mg/yolk100 g) and there were no price differences between vitamin E enriched eggs and ordinary eggs. In the contents of vitamin E, docosahexaenoic acid (DHA) and cholesterol, there were no significant differences according to the price. These results suggests that egg production, distribution, and management system should be improved for supplying fresh eggs to consumers at reasonable prices.