• Journal of the Korea Society of Computer and Information
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• v.22 no.7
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• pp.83-91
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• 2017

In this paper, we propose a thorough solution for adjusting virtual sound field with different kinds of devices and software in preliminary procedure and late stage of music processing. The basic process of music production includes composing, arranging and recording at pre-production stage as well as sound mixing and mastering at post-production stage. At the initial stage of music creation, it should be checked whether the design of virtual sound field, the choice of the tone and the instrument used in the arrangement match the virtual sound field required for the final work. In later recording, mixing and mastering, elaborate adjustments should be done to the virtual sound field. This study also analyzed how to apply the parameter of the effectors to the design and adjustment of the virtual sound field, making it the source of our creation.

• Journal of Applied Biological Chemistry
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• v.56 no.1
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• pp.37-42
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• 2013

In eukaryotes, most of the genome are transcribed, however only a small proportion of total transcripts encodes for protein, thus resulting in many of noncoding RNAs. In order to recover DNA damage including DNA double-strand breaks (DSBs) eukaryotes have evolved complex mechanisms and these are processed through coordinated mechanisms of protein sensors, transducers, and effectors including RNAs. During recent years, small RNAs have been increasingly studied and gradually considered as key regulators in various aspects of biology. Upon DNA damage, small RNAs including diRNAs (DSB induced RNA) are generated in both plant and human cell lines. Inhibition of their biogenesis has severe influence on DSB repair system.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.563-567
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• 2014

Pseudomonas syringae pv. syringae (Psy) is a major bacterial pathogen of many economically important plant species. Despite the severity of its impact, the genome sequence of the type strain has not been reported. Here, we present the draft genome sequence of Psy ATCC 19310. Comparative genomic analysis revealed that Psy ATCC 19310 is closely related to Psy B728a. However, only a few type III effectors, which are key virulence factors, are shared by the two strains, indicating the possibility of host-pathogen specificity and genome dynamics, even under the pathovar level.

• Journal of the Korean Society of Systems Engineering
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• v.18 no.2
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• pp.126-139
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• 2022

The need for synthetic automation is increasing in preparation for a gradual decrease in laboratory research manpower due to low birth rate and aging. In this study, the existing laboratory synthesis method is analyzed based on the systems engineering technique. Then, it led to the derivation of the system requirements for a fixed-based robot manipulator capable of recognition, decision and control. The robot is equipped with replaceable modular end-effectors and designed depending on the purpose and process of the synthesis. The robot with an end-effector was implemented as PoC(Proof-of-Concept), and the functions for pipetting automation was verified.

• The Plant Pathology Journal
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• v.36 no.3
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• pp.303-303
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• 2020

• The Plant Pathology Journal
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• v.38 no.1
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• pp.12-24
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• 2022

In this study, we conducted whole-genome sequencing with six species of Pectobacterium composed of seven strains, JR1.1, BP201601.1, JK2.1, HNP201719, MYP201603, PZ1, and HC, for the analysis of pathogenic factors associated with the genome of Pectobacterium. The genome sizes ranged from 4,724,337 bp to 5,208,618 bp, with the GC content ranging from 50.4% to 52.3%. The average nucleotide identity was 98% among the two Pectobacterium species and ranged from 88% to 96% among the remaining six species. A similar distribution was observed in the carbohydrate-active enzymes (CAZymes) class and extracellular plant cell wall degrading enzymes (PCWDEs). HC showed the highest number of enzymes in CAZymes and the lowest number in the extracellular PCWDEs. Six strains showed four subsets, and HC demonstrated three subsets, except hasDEF, in type I secretion system, while the type II secretion system of the seven strains was conserved. Components of human pathogens, such as Salmonella pathogenicity island 1 type type III secretion system (T3SS) and effectors, were identified in PZ1; T3SSa was not identified in HC. Two putative effectors, including hrpK, were identified in seven strains along with dspEF. We also identified 13 structural genes, six regulator genes, and five accessory genes in the type VI secretion system (T6SS) gene cluster of six Pectobacterium species, along with the loss of T6SS in PZ1. HC had two subsets, and JK2.1 had three subsets of T6SS. With the GxSxG motif, the phospholipase A gene did locate among tssID and duf4123 genes in the T6SSa cluster of all strains. Important domains were identified in the vgrG/paar islands, including duf4123, duf2235, vrr-nuc, and duf3396.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.11
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• pp.11-21
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• 1996

Robot manipulators constituing multi-robot system must exert the desired motion force on an object to preserve tghe fine motion of it. The forces exerte on an object by the end-effectors of multi-inducing force and the internal force. Here, motion-inducing force effects the motion of an object, but internal force as lies in the null space of an object coordinate can't effect it. The motion of an object can't track exactly the desired motion by the effect of an object, but internal force as lies in the null space of the effect of internal force component, therefore internal force component must be considered. In this paper, first, under assumption that we can estimate exactly the parameter of dynamics, we constitute paper, first, under assumption that we can estimate exactly the parameter of dynamics, we constitute the controller concerning internal force. And we obtain the internal force as projecting force sensor readings onto the space spanned by null basis set of jacobian matrix. Using the resolved acceleration control method and the fact that internal force lies in the null space of jacobian matrix, we construct the robust control law to preserve the robustness with respect to the uncertainty of mainpulator parameters.

• The Journal of Korea Robotics Society
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• v.13 no.3
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• pp.164-173
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• 2018

This paper proposes functional test methods of first-aid gadgets which are special end-effectors, for relief robot. In recent years, researches have been actively conducted on robots that can perform rescue operations on behalf of rescue workers in dangerous areas such as disasters and wars. These special robots mainly perform the task of finding or transporting injured people. However, it is sometimes they necessary to provide first aid in the field. Among the various first-aid operations, gadgets are being developed for oxygen supply, injection, and hemostasis operations that can be used in a defense/civilian area by using robot technology. Previous studies have proposed first-aid gadgets that are suitable for onsite situations and enable robots to perform the given task quickly and accurately. In this paper, we design a test procedure suitable for the functions of first-aid gadgets, summarize the results, and introduce future research directions.

• BMB Reports
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• v.34 no.3
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• pp.212-218
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• 2001

Although the blockage of a cell cycle by specific inhibitors of $Ca^{2+}/$calmodulin-dependent protein kinase II (CaMK-II) is well known, the activity profile of CaMK-II during the cell cycle in the absence of any direct effectors of the enzyme is unclear. The activity of native CaMK-II in NIH 3T3 cells was examined by the use of cell cycle-specific arresting and synchronizing methods. The total catalytic activity of CaMK-II in arrested cells was decreased about 30% in the M phase, whereas the $Ca^{2+}$-independent autonomous activity increased about 1.5-fold in the M phase and decreased about 50% at the G1/S transition. The in vivo phosphorylation level of CaMK-II was lowest at G1/S and highest in M. The CaMK-II protein level was unchanged during the cell cycle. When the cells were synchronized, the autonomous activity was increased only in M. These results indicate that the physiologically relevant portion of CaMK-II is activated only in M, and that the net activation of CaMK-II is required in mitosis.

• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.610-615
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• 2017

When Shigella infect host cells, various effecter molecules are delivered into the cytoplasm of the host cell through the type III secretion system (TTSS) to facilitate their invasion process and control the host immune responses. Among these effectors, the S. flexneri effector OspF dephosphorylates mitogen-activated protein kinases and translocates itself to the nucleus, thus preventing histone H3 modification to regulate expression of proinflammatory cytokines. Despite the critical role of OspF, the mechanism by which it localizes in the nucleus has remained to be elucidated. In the present study, we identified a potential small ubiquitin-related modifier (SUMO) modification site within OspF and we demonstrated that Shigella TTSS effector OspF is conjugated with SUMO in the host cell and this modification mediates the nuclear translocation of OspF. Our results show a bacterial virulence factor can exploit host post-translational machinery to execute its intracellular trafficking.