• Journal of the Korean Institute of Telematics and Electronics
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• v.24 no.1
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• pp.149-158
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• 1987

This paper describes the SEAS which is a 2-dimensional graphic editor for schematic circuit and IC mask layout. This system runs on general purpose computer and Tektronix 4110 series graphic terminal. With this system, user can edit schematic circuits and/or IC mask layout with 2 level hierarchy. This system supports more than 20 kinds of built-in symbol, user definable internal symbols, 60 macros, 16 function keys, 4 level on-screen menu operation and edit-in-place of cell in main drawing. And it provides editing functions such as reflection, rotation, move and copy of one or a group of elements, and modification of polygona geometry etc. To improve the excution speed, we used local memory of graphic terminal. For the portability of the program, the system is written in FORTRAN 77 programming language.

• Journal of information and communication convergence engineering
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• v.9 no.1
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• pp.110-116
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• 2011

The production environment of fast and accurate news video has been continuously changing together with technology advance. Especially, the editing of the news video advanced focusing on the non-linear system by the introduction of digital technology and the recording of news video advanced to 'tapeless' base. In this new news video production environment, the recorded news videos are transmitted, edited and archived through the NPS (Network Production System), which dramatically improves the efficiency of the news video production. However, there are some issues which need to be resolved in the introduction of NPS. The purpose of this research is to identify the necessity of NPS introduction in the changing news video production environment, explore the issues which should be resolved and suggest the proper way to introduce the NPS to broadcasting companies.

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1583-1591
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• 2020

CRISPR/Cpf1 has emerged as a new CRISPR-based genome editing tool because, in comparison with CRIPSR/Cas9, it has a different T-rich PAM sequence to expand the target DNA sequence. Single-base editing in the microbial genome can be facilitated by oligonucleotide-directed mutagenesis (ODM) followed by negative selection with the CRISPR/Cpf1 system. However, single point mutations aided by Cpf1 negative selection have been rarely reported in Corynebacterium glutamicum. This study aimed to introduce an amber stop codon in crtEb encoding lycopene hydratase, through ODM and Cpf1-mediated negative selection; deficiency of this enzyme causes pink coloration due to lycopene accumulation in C. glutamicum. Consequently, on using double-, triple-, and quadruple-base-mutagenic oligonucleotides, 91.5-95.3% pink cells were obtained among the total live C. glutamicum cells. However, among the negatively selected live cells, 0.6% pink cells were obtained using single-base-mutagenic oligonucleotides, indicating that very few single-base mutations were introduced, possibly owing to mismatch tolerance. This led to the consideration of various target-mismatched crRNAs to prevent the death of single-base-edited cells. Consequently, we obtained 99.7% pink colonies after CRISPR/Cpf1-mediated negative selection using an appropriate single-mismatched crRNA. Furthermore, Sanger sequencing revealed that single-base mutations were successfully edited in the 99.7% of pink cells, while only two of nine among 0.6% of pink cells were correctly edited. The results indicate that the target-mismatched Cpf1 negative selection can assist in efficient and accurate single-base genome editing methods in C. glutamicum.

• Molecules and Cells
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• v.40 no.11
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• pp.823-827
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• 2017

Genome editing using programmable nucleases such as CRISPR/Cas9 or Cpf1 has emerged as powerful tools for gene knock-out or knock-in in various organisms. While most genetic diseases are caused by point mutations, these genome-editing approaches are inefficient in inducing single-nucleotide substitutions. Recently, Cas9-linked cytidine deaminases, named base editors (BEs), have been shown to convert cytidine to uridine efficiently, leading to targeted single-base pair substitutions in human cells and organisms. Here, we first report on the generation of Xenopus laevis mutants with targeted single-base pair substitutions using this RNA-guided programmable deaminase. Injection of base editor 3 (BE3) ribonucleoprotein targeting the tyrosinase (tyr) gene in early embryos can induce site-specific base conversions with the rates of up to 20.5%, resulting in oculocutaneous albinism phenotypes without off-target mutations. We further test this base-editing system by targeting the tp53 gene with the result that the expected single-base pair substitutions are observed at the target site. Collectively, these data establish that the programmable deaminases are efficient tools for creating targeted point mutations for human disease modeling in Xenopus.

• Herbal Formula Science
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• v.21 no.1
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• pp.142-153
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• 2013

Objectives : Examined the editing system and the characteristics of "Clinical Formula Science of Korean Medicine" to establish a basis for compilation system of the common formula science textbook for colleges of Korean medicine nationwide. Methods : Comprehended the "Clinical Formula Science of Korean Medicine" published by professor Hyeongok(1911-1987) in 1985 and analyzed its configuration system. Results : "Clinical Formula Science of Korean Medicine" has made it possible to understand the formula systematically by establishing and classifying 24 kinds of treatment method [the three treatment methods of cold-dampness, dryness-heat and interior deficiency and its subdivision, 19 kinds of treatment method (stomach, phlegm, eum, yang, spirit, essence, ki, blood, blood stasis, exterior, interior, half-exterior half-interior, water, wind, cold, heat, dampness) combined with reducing and vomiting] and main formula. These can be considered as professor Hyeongok's own method of classification, which is distinctive from the existing formula science system. Also, it suggests that a Korean medicine doctor should not use a biased formula science that is limited to some schools but apply the formula widely and synthetically because it is believed to pursue perfection in cure if the main formulas related to a physical disorder, pregnancy and childbirth, invigorating and purging five viscera, and Four-Constitution Medicine are added. Conclusions : "Clinical Formula Science of Korean Medicine" is seen as a formula science book which is to be consulted when compiling a common formula science textbook for colleges of Korean medicine by forming a Korean formula science system that is distinctive from that of China.

• Genomics & Informatics
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• v.18 no.1
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• pp.8.1-8.10
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• 2020

The explosive growth of next-generation sequencing data has resulted in ultra-large-scale datasets and ensuing computational problems. In Korea, the amount of genomic data has been increasing rapidly in the recent years. Leveraging these big data requires researchers to use large-scale computational resources and analysis pipelines. A promising solution for addressing this computational challenge is cloud computing, where CPUs, memory, storage, and programs are accessible in the form of virtual machines. Here, we present a cloud computing-based system, Bio-Express, that provides user-friendly, cost-effective analysis of massive genomic datasets. Bio-Express is loaded with predefined multi-omics data analysis pipelines, which are divided into genome, transcriptome, epigenome, and metagenome pipelines. Users can employ predefined pipelines or create a new pipeline for analyzing their own omics data. We also developed several web-based services for facilitating downstream analysis of genome data. Bio-Express web service is freely available at https://www. bioexpress.re.kr/.

• Journal of the Korea Society of Computer and Information
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• v.12 no.6
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• pp.287-295
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• 2007

This paper proposes a novel data editing techniques with genetic algorithm (GA) in case-based reasoning (CBR) for the prediction of Korea Stock Price Index (KOSPI). CBR has been widely used in various areas because of its convenience and strength in compelax problem solving. Nonetheless, compared to other machine teaming techniques, CBR has been criticized because of its low prediction accuracy. Generally, in order to obtain successful results from CBR, effective retrieval of useful prior cases for the given problem is essential. However. designing a good matching and retrieval mechanism for CBR system is still a controversial research issue. In this paper, the GA optimizes simultaneously feature weights and a selection task for relevant instances for achieving good matching and retrieval in a CBR system. This study applies the proposed model to stock market analysis. Experimental results show that the GA approach is a promising method for data editing in CBR.

• Journal of Korea Multimedia Society
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• v.9 no.5
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• pp.529-541
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• 2006

In this paper, we propose a semi-automatic segmentation method which can be used to generate video object plane (VOP) for object based coding scheme and multimedia authoring environment. Semi-automatic segmentation can be considered as a user-assisted segmentation technique. A user can initially mark objects of interest around the object boundaries and then the selected objects are continuously separated from the un selected areas through time evolution in the image sequences. The proposed segmentation method consists of two processing steps: partially manual intra-frame segmentation and fully automatic inter-frame segmentation. The intra-frame segmentation incorporates user-assistance to define the meaningful complete visual object of interest to be segmentation and decides precise object boundary. The inter-frame segmentation involves boundary and region tracking to obtain temporal coherence of moving object based on the object boundary information of previous frame. The proposed method shows stable and efficient results that could be suitable for many digital video applications such as multimedia contents authoring, content based coding and indexing. Based on this result, we have developed objects based video editing system with several convenient editing functions.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.179-185
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• 2021

BACKGROUND: Tomato genome editing using CRISPR-Cas9 is being actively conducted in recent days, and lots of plant researches have been aiming to develop high valued crops by editing target genes without inserting foreign genes. Many researchers have been involved in the manipulation of the crop ripening process because fruit ripening is an important fruit phenotype for increasing fruit shelf life, taste, and texture of crops. This paper intends to evaluate target sgRNA to edit the two ripening-related genes encoding pectate lyase (PL) and phytoene synthase (Psy) with the CRISPR-Cas9 system. METHODS AND RESULTS: The CRISPR-Cas9 expression vector was cloned to target the PL (Solyc03g111690), Psy1 (Solyc03g031860), and Psy2 (Solyc02g081330) genes, which are the ripening genes of tomatoes. Tomatoes injected with Agrobacterium containing the CRISPR-Cas9 expression vector were further cultured for 5 days and used to check gene editing efficiency. As a result of the target gene sequence analysis by the next generation sequencing method, gene editing efficiency was calculated, and the efficient target location was selected for the PL and Psy genes. CONCLUSION: Therefore, this study was aimed to establish target sgRNA data that could have higher efficiency of the CRISPR-Cas9 system to obtain the delayed ripening phenotype of tomato. The developed method and sgRNA information is expected to be utilized in the development of various crops to manage its ripening processes.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.3
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• pp.28-37
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• 1996

To reduce an authors burden in hypermedia system that allows non-sequential information the process of creating links must be easy. However, most of the conventional hypermedia systems possess two difficulties. First, the author must go through several troublesome process to create a single link. Secondly, it is not easy to create an anchor in text or other multimedia data. Therefore, in order to support effective construction of hypermedia system the editing environment must provide an easy method to create links. In this paper, to resolve the weaknesses of conventional hypermedia system as mentioned above, an editing tool is developed and implemented to easily create the links of multimedia data. There are three methods in creating links and a user can select a convenient method in given circumstances. And for teh efficient production of nodes composed of multimedia information, we provide an authoring environment to integrate and process those informations.