• Archives of Craniofacial Surgery
• /
• 제24권4호
• /
• pp.198-201
• /
• 2023

Maintaining the patency of the external auditory canal (EAC) during reconstruction is important because of its physiological role in hearing and immunological protective functions. The curved shape of the EAC presents a challenge when performing a skin graft. One of the key points for a successful skin graft is to ensure compression on the wound bed, and many novel methods, including prefabricated ear molds, have been reported for this purpose. In this study, we present a case of a skin graft performed to reconstruct a skin defect following excision of actinic keratosis in the EAC, using the cover of an ear thermometer probe as a mold for the graft to match the curvature of the EAC. This is an economical and practical method for secure compression dressing of a skin graft in the EAC.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• 제58권3호
• /
• pp.267-273
• /
• 2013

We evaluated the morphological characteristics of 79 popcorn inbred lines, which were developed to breeding popcorn variety at Maize Experiment Station, Gangwon Agricultural Research and Extension Services, by examining ten quantitative and three qualitative characteristics. In the survey of 3 qualitative traits for 79 popcorn inbred lines, most inbred lines respectively showed purple (46 inbred lines) at tassel color (QL1), green (55inbred lines) at silk color (QL2) and green (75 inbred lines) at stem color (QL3). While, on the survey of 10 quantitative traits among 79 popcorn inbred lines, they showed the morphological variation in plant height (QN1, $174.2{\pm}34.9$ cm), ear height (QN2, $103.4{\pm}24.7$ cm), ear length (QN3, $9.4{\pm}3.0$ cm), kernel setting length (QN4, $8.4{\pm}2.6$ cm), ear thickness (QN5, $24.9{\pm}7.7$ mm), ear row number (QN6, $14.0{\pm}2.3$ number), ear weight (QN7, $36.5{\pm}26.0$ g), kernel weight (QN8, $30.9{\pm}19.3$ g), 100 kernel weight (QN9, $10.4{\pm}3.8$ g) and germination rate (QN10, $95.3{\pm}8.1%$), respectively. As a result, 5 inbred lines (PS0-001, PS0-003, PS1-002, PS1-003, PS2-009) in the 79 popcorn inbred lines have showed comparatively high values for the five quantitative traits. On the principal component analysis, silk color (QL2), ear length (QN3), kernel setting length (QN4), ear thickness (QN5), ear weight (QN7), kernel weight (QN8) and 100 kernel weight (QN9) greatly contributed in positive direction on the first principal components, whereas tassel color (QL1), stem color (QL3), ear height (QN2) and ear row number (QN6) contributed in negative direction on the first principal component. In addition, plant height (QN1), ear height (QN2), and kernel weight (QN8) contributed in positive direction on the second principal component, and also tassel color (QL1), silk color (QL2), ear length (QN3), ear row number (QN6) and 100 kernel weight (QN9) contributed in negative direction on the second principal component.

• Journal of Ginseng Research
• /
• 제45권5호
• /
• pp.610-616
• /
• 2021

Background: Thymic stromal lymphopoietin (TSLP) acts as a master switch for inflammatory responses. Ginsenoside Rg3 (Rg3) which is an active ingredient of Panax ginseng Meyer (Araliaceae) is known to possess various therapeutic effects. However, a modulatory effect of Rg3 on TSLP expression in the inflammatory responses remains poorly understood. Methods: We investigated antiinflammatory effects of Rg3 on an in vitro model using HMC-1 cells stimulated by PMA plus calcium ionophore (PMACI), as well as an in vivo model using PMA-induced mouse ear edema. TSLP and vascular endothelial growth factor (VEGF) levels were detected using enzyme-linked immunosorbent assay or real-time PCR analysis. Murine double minute 2 (MDM2) and hypoxia-inducible factor 1α (HIF1α) expression levels were detected using Western blot analysis. Results: Rg3 treatment restrained the production and mRNA expression levels of TSLP and VEGF in activated HMC-1 cells. Rg3 down-regulated the MDM2 expression level increased by PMACI stimulation. The HIF1α expression level was also reduced by Rg3 in activated HMC-1 cells. In addition, Rg3-administered mice showed the decreased redness and ear thickness in PMA-irritated ear edema. Rg3 inhibited the TSLP and VEGF levels in the serum and ear tissue homogenate. Moreover, the MDM2 and HIF1α expression levels in the ear tissue homogenate were suppressed by Rg3. Conclusion: Taken together, the current study identifies new mechanistic evidence about MDM2/HIF1α pathway in the antiinflammatory effect of Rg3, providing a new effective therapeutic strategy for the treatment of skin inflammatory diseases.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• 제33권3호
• /
• pp.1-26
• /
• 2020

Objectives : This study was to examine the effects of 3 types of BTS which were excluded talcum only or replaced talcum to Lonicera japonicae Flos or Kochiae Fructus on the DNCB-induced atopic dermatitis in mice. Methods : In this study, Balb/c mice were divided into five groups: normal, control, GBT(BTS except talcum), GBTG(GBT added Lonicera japonicae Flos), and GBTJ(GBT added Kochiae Fructus). And the effects on atopic dermatitis were evaluated by weight change, ear's thickness and weight, thickness of dorsal skin, severity scale of dorsal skin, histopathologic findings of dorsal skin by H&E and toluidine blue stain, proliferation of splenocyte and thymocyte in vitro, proliferation of splenocyte in vivo, IL-4, TNF-α, IgE in serum. Results : There were no significantly changes in body weight and effect of ear's weight in GBT, GBTG, and GBTJ group. The thickness of ear of GBT and GBTJ group showed significant decrease. And the thickness of dorsal skin of GBTJ group significantly decreased compared to the control, GBT, and GBTG group. All the treated groups significantly decreased in severity scale, histopathologically reduced epidermal thickness, and mast cell infiltration. In vitro, all the treated groups increased in the proliferation rates of splenocyte. However, in vivo study, it showed a falling tendency and GBT group significantly decreased compared to control, GBTG, and GBTJ group. In vitro study, GBTG group significantly decreased in the proliferation rates of thymocyte. There was no IgE contents chnage in GBT, GBTG, and GBTJ groups but IL-4 and TNF-α contents were significantly decreased. Conclusions : GBT, GBTG, and GBTJ are expected to improve symptoms of atopic dermatitis and further studies are needed for development of BTS's transformation.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• 제35권2호
• /
• pp.117-121
• /
• 1990

A sweet corn hybrid, Golden Cross Bantam 70, was grown at 5 plant populations (5,000, 6,000, 7,000, 8,000, and 9,000 plants/10a) under the transparent P .E. film mulch to find the best yield evaluation method. Plant population did not affect early plant growth, culm length, ear height, and silking date. However, number of tillers at harvest decreased but leaf area index increased with increased plant population. Marketable ears were divided into two classes; the first grade of which husked ear weight over l50g (unhusked ear weight 230g) and the second grade of which husked ear weight between 100 and 150g (unhusked ear weight between 180 and 230g) according to the whole sale market price. Average length, thickness, and weight of marketable ears over 100g of husked ears decreased with increased plant population. The proportion of ears over 150g decreased with increased plant population. However, total number and weight of marketable ears and gross income per 10a calculated considering weight and number of ears increased with plant population. There were highly positive correlations between gross income and ear number or ear weight per unit area. At high plant populations the number of marketable ears was overestimated but ear weight underestimated compared with gross income. Dry matter yield of stover increased with increased plant population and ranged 755-944kg/10a with 20.7-24.5% dry matter content. Rice black-streaked dwarf virus infection rate was 10.6-14.9%, but it was not related to plant population.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• 제11권1호
• /
• pp.63-71
• /
• 2017

In this paper, a signal processing circuit for semi-implantable middle ear hearing device is designed using the TCBT which is recently proposed for a new middle ear transducer that can be implanted at round window of cochlea. The designed semi-implantable hearing device transmits digital sound signal from external device located at behind the ear to the internal device implanted under the skin using inductive coupling link methods with high efficiency. The coils and signal processing circuits are designed and implemented considering the total transmission and reception distance including skin thickness of temporal bone for the semi-implantable hearing device. And also, to improve the data transmission efficiency, the output circuits which can supply sufficient signal power is designed. In order to confirm operation of semi-implantable hearing device using inductive coupling link, the circuit analysis was performed using PSpice, and the performance was verified by implementing a signal processing board of an available size.

• Journal of Physiology & Pathology in Korean Medicine
• /
• 제26권5호
• /
• pp.693-698
• /
• 2012

This study was designed to investigate the effects of 1-fluoro-2,4 dinitrobenzene (DNFB) on Induction of dermatitis in mice. We investigated the effects of DNFB on induction of dermatitis in terms of changes in body weights, ear thickness, ear weight, spleen/body ratio, histopathological observation and cytokine productions in inflammed tissue of contact dermatitis (CD) mice. In our experiments, we induced CD by using two different methods. First, mice were sensitized and challenged on the back of each ear (topical induction). Second, mice were sensitized on shaved back and challenged back of each ear (systemic induction). In our results, average weights were lowered in both topical and systemic group. But, there was no statistical significance between topical and systemic group. Treatment with DNFB enlarged ear weights and thicknesses in both topical and systemic groups. In addition, both groups were showed almost same features such as immune cell infiltration, spongiosis and hyperplasia in histopathological observations. Finally production levels of TNF-a, IFN-g and IL-6 were markedly elevated in both topical and systemic groups. On the other hand, systemic induction was more effective in spleen/body ratio and IL-6 production compared to topical induction respectively. These results suggest that dermatitis model using DNFB was used as model of CD, not for atopic dermatitis model.

• Journal of Physiology & Pathology in Korean Medicine
• /
• 제20권3호
• /
• pp.623-628
• /
• 2006

Sopung-san(SPS; prescription No. DO27), a herbal medicine prescribed by oriental hospital of Daegu fanny University, has been used as cure for pruritus and psoriasis. To evaluate anti-inflammatory effect of Sopung-san, we treated Sopung-san extract in animal model system induced contact hypersensitivity. Contact hypersensitivity, a local inflammatory response of the skin, was induced by 1% DNCB on the right ear of BALB/C mouse. Sopung-san was prepared as water extract and administrated everyday for 2 weeks per oral. A right ear of mouse was potently swelled by 1% of DNCB treatment, but a mouse ear thickness was significantly reduced by Sopung-san after 2 weeks. treatment. Sopung-san reduced IgG in serum obtained from blood of 1 % DNCB-treated mouse. IgE in serum was not changed by DNCB and/or Sopung-san treatment. $IL-1{\beta}$ and IL-6 from ear tissue were significantly increased by DNCB, Dut were not reduced by Sopung-san. From these results, anti-inflammatory effect of Sopung-san, especially reduction of ear swelling, might be partly due to reduction of IgG in serum.

• Journal of Acupuncture Research
• /
• 제38권4호
• /
• pp.293-299
• /
• 2021

Background: This study sought to determine whether the antioxidant effects of astaxanthin (AST) could have an anti-inflammatory effect to reduce inflammation caused by atopic dermatitis (AD). Methods: Using a mouse model of AD induced by phtalic acid (PA), the levels of inflammation, inflammatory agents, and evidence of antioxidant activity were examined in PA treated mice (n = 3), PA-AST treated mice (n = 3), and a control group of mice (n = 3). This included measurements of ear thickness, levels of mast cells, IgE, inflammatory cytokine, malondialdehyde (MDA), hydrogen peroxide, HO-1, and GPx-1. Results: AST treatment significantly prevented inflammation as measured by ear thickness (p < 0.05), mast cell count (p < 0.001), and IgE concentration in the blood (p < 0.001). Levels of TNF-α (p < 0.001), IL-1β (p < 0.001), IL-6 (p < 0.001), and MDA (p < 0.05) were also significantly lower. In addition, GSH levels increased significantly (p < 0.001), and the level of hydrogen peroxide significantly reduced (p < 0.01). The expression of HO-1, GPx-1 increased. Conclusion: In this small experimental study, AST acted on inflammatory mechanisms that induced AD, through anti-inflammatory and antioxidant mechanisms, and is a candidate of interest in the clinical treatment of AD.

• Korean Journal of Exercise Nutrition
• /
• 제24권4호
• /
• pp.7-14
• /
• 2020

[Purpose] This study evaluated the anti-atopic dermatitis (AD) properties of Cordyceps militaris (CM) aqueous extract in keratinocytes in vitro and in vivo. We investigated the nutraceutical composition of the CM extract, including its protein, carbohydrate, and selected phytochemical content. [Methods] The expression of pathogenic cytokines in keratinocytes was assayed using an in vitro model. The CM extract downregulated extracellular signalregulated kinase 1/2 (ERK1/2) and p38 kinase expression in TNFα/IFNγ-stimulated HaCaT cells. We also established an in vivo AD model by repeatedly exposing the ears of mice to local Dermatophagoides farinae extract (DFE; house dust mite extract) and 2,4-dinitrochlorobenzene (DNCB). The epidermal and dermal ear thickness, mast cell infiltration, and serum immunoglobulin levels were measured following a 4-week oral administration of the CM extract. [Results] Histopathological examination showed reduced epidermal/dermal thickness and mast cell infiltration in mouse ears. The CM extract also suppressed serum immunoglobulin levels and gene expression of T helper (Th)1/Th2 cytokines in mouse ear tissue. [Conclusion] These results suggest that the CM extract may be useful for the treatment of AD-like skin lesions.