• Korean Journal of Microbiology
• /
• v.28 no.2
• /
• pp.91-97
• /
• 1990

Complementary DNA (cDNA) coding for human cytoplasmic superoxide dismutase (SOD1) (superoxide: superoxide oxidoreductase E.C.1.15.1.1) was isolated from human liver cDNA library of $\lambda$gt11 by in situ plaque hybridization. The insery cDNA gas the 5' untranslational region (UTR) and 3'UTR of SOD1 gene. Polymerase Chain Reaction (PCR) method was used fro subcloning of SOD1 structural gene. Using synthetic sense strand primer (24mer) containing a start codon and antisense strand primer (24mer), SOD1 structural gene was selectively amplified. Amplified DNA was directly cloned into the HincII site of pUC19 plasmid. Insery cDNA was subcloned into M13 mp19 and sequenced by dideowy chain termination method with Sequenase. The nucleotide sequence of insert cDNA had an open reading frame (ORF) coding for 153 amino acid residues. The structural gene of cytoplasmic SOD was placed under the control of bacteriophage $\lambda P_{L}$ regulatory sequences, generating a highly efficient expression plasmid. The production of human SOD1 in E. coli cells was about 7% of total cellular proteins and recombinant human SOD1 possessed its own enzymatic acitivity.

• The KIPS Transactions:PartD
• /
• v.11D no.1
• /
• pp.195-202
• /
• 2004

This paper describes the development of a window manager based on the real time operating system, Qplus-p, for small internet appliance. Current window managers for small internet appliance are originally developed for desktop environment and then modified for embedded systems. So they have inadequate functions for small internet appliances and do not perfectly support Hangul. The Qplus-P window manager adds the Hangul processing function to Matchbox window manager, a royalty-free window manager for small Internet appliance and provides a new Hangul input method called Amikey. Input mode can be changed automatically from Hangul mode to English mode and vice versa by Amikey. In addition, Qplus-P window manager provides Hangul supported basic applications such as web browser, e-mail client, MP3 player. We developed the window manager by porting open sources to target system after adding Hangul supported functions to them.

• Journal of Families and Better Life
• /
• v.27 no.5
• /
• pp.25-42
• /
• 2009

This study examines the actual state of using digital convergence products by consumers, and analyzes the expectation that consumers have regarding digital convergence products and the actual satisfaction of the digital convergence products. In addition, it analyzes factors affecting the satisfaction with digital convergence products. First, mobile phones, which 99.4% of those surveyed had, cameras, which 90.6% had, and mp3s, which 71.8% had, were found to be necessities. Second, the ratio of owning digital convergence products was 79.5%, 48.1%, and 52.4%, respectively. In the case of a mobile phones, a digital convergence type is already common. Third, regarding the reason for purchasing digital convergence products, they chose convergence products because there was no product with a simple function that they wanted to have: 38.2% in terms of a mobile phone, 47.2% for a camera, and 28.2% for an mp3. This shows that a consumer's choice was infringed in a great deal at the back of remarkable quantitative growth in digital convergence products. Fourth, the analysis of the factor regarding the consumer's expectation on digital convergence products showed 3 kinds of factors, i.e., expectation of benefit, expectation of risk, and expectation of situation. Fifth, as a result of analyzing the structural model regarding the effect on satisfaction by a consumer's expectation and the outcome on digital convergence products, all the expectations and outcomes of benefit, risk, and situation did not have a direct effect on satisfaction. Only an indirect effect through inconsistency showed a statistical significance.

• Journal of Environmental Science International
• /
• v.25 no.1
• /
• pp.67-83
• /
• 2016

In order to improve the prediction of the regional air quality modeling in the Seoul metropolitan area, a sensitivity analysis using two PBL and microphysics (MP) options of the WRF model was performed during four seasons. The results from four sets of the simulation experiments (EXPs) showed that meteorological variables (especially wind field) were highly sensitive to the choice of PBL options (YSU or MYJ) and no significant differences were found depending on MP options (WDM6 or Morrison) regardless of specific time periods, i.e. day and night, during four seasons. Consequently, the EXPs being composed of YSU PBL option were identified to produce better results for meteorological elements (especially wind field) regardless of seasons. On the other hand, the accuracy of all simulations for summer and winter was somewhat lower than those for spring and autumn and the effect according to physics options was highly volatile by geographical characteristics of the observation site.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.10a
• /
• pp.211-215
• /
• 2015

It comes into the spotlight as the new Blue Ocean in which the connected car industry in which the car and mobile communication technology is convergence. All sorts of infortainments services connecting with the portable electronic device(Smart phone, tablet PC, and MP3 player) and car are rapidly grown. The Connected car emphasizes the vehicle connectivity with the concept that the car has communication with the around on a real time basis and it provides the safety and expedience to the operator and using the thing of Internet (IoT) in the car and supports the application, presently, the entertainment service including the real-time Navigation, parking assistant function, not only the remote vehicle control and management service but also Email, multimedia streaming service, SNS and with the platform. Intelligent vehicle network is studied as the kind according to MANET(Mobile Ad Hoc Network) for the safety operation of the cars of the road and improving the efficiency of the driving. The intelligent vehicle network is comprised for the driving information offering changing rapidly of the communication(V2V: Vehicle to Vehicle) between the car and the car, communication(V2I : Vehicle to Infrastructure) between the infrastructure and the car, and V2X (Vehicle to Nomadic).

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.114.1-114
• /
• 2003

A new isolate of rod-shaped virus was identified from grafted cactus, Gymnocalycium mihanovichii grafted onto Hylocereus trigonus, in Korea. The virus proved to be a new Tobamovirus and called previously as Tobamovirus-Ca for which we suggest the name Cactus mild mottle virus(CMMoV), because it produced systemic mild mosaic symptoms on its original host. CMMoV is distantly related to known species of the genus Tobamovirus on the basis of host range, serological and sequence analyses. Western blot analysis showed that CMMoV is serologically unrelated to Summons' Opuntia virus which is the only known species of the genus found in cactus plants. The 3'-terminal 2,910 nucleotides have been sequenced for the virus. The coat protein (CP) and movement protein (MP) genes encode 161 and 306 amino acids residues, respectively. The nucleotide and amino acid sequences of the CP were 39.6 ％ to 49.2 ％ and 26.4 ％ to 40.3 ％ identical to other tobamoviruses, respectively. The MP and 3' noncoding region shared 16.3 ％ to 23.3 ％ and 44.6 ％ to 63.4 ％ identities, respectively, with the members of the genus. Phylogenetic tree analysis of the CP gene revealed that CMMoV clusters with members of subgroup I of Tobamovirus. CMMoV particles contained genomic RNA along with two subgenomic RNAs, and this characteristics is common in the members of the subgroup II. This is the first information of sequence and comparative analysis of a Tobamovirus that infects cactus.

• IMMUNE NETWORK
• /
• v.23 no.2
• /
• pp.16.1-16.19
• /
• 2023

Bacillus Calmette-Guerin (BCG) vaccine is the only licensed vaccine for tuberculosis (TB) prevention. Previously, our group demonstrated the vaccine potential of Rv0351 and Rv3628 against Mycobacterium tuberculosis (Mtb) infection by directing Th1-biased CD4⁺ T cells co-expressing IFN-γ, TNF-α, and IL-2 in the lungs. Here, we assessed immunogenicity and vaccine potential of the combined Ags (Rv0351/Rv3628) formulated in different adjuvants as subunit booster in BCG-primed mice against hypervirulent clinical Mtb strain K (Mtb K). Compared to BCG-only or subunit-only vaccine, BCG prime and subunit boost regimen exhibited significantly enhanced Th1 response. Next, we evaluated the immunogenicity to the combined Ags when formulated with four different types of monophosphoryl lipid A (MPL)-based adjuvants: 1) dimethyldioctadecylammonium bromide (DDA), MPL, and trehalose dicorynomycolate (TDM) in liposome form (DMT), 2) MPL and Poly I:C in liposome form (MP), 3) MPL, Poly I:C, and QS21 in liposome form (MPQ), and 4) MPL and Poly I:C in squalene emulsion form (MPS). MPQ and MPS displayed greater adjuvancity in Th1 induction than DMT or MP did. Especially, BCG prime and subunit-MPS boost regimen significantly reduced the bacterial loads and pulmonary inflammation against Mtb K infection when compared to BCG-only vaccine at a chronic stage of TB disease. Collectively, our findings highlighted the importance of adjuvant components and formulation to induce the enhanced protection with an optimal Th1 response.

• Parasites, Hosts and Diseases
• /
• v.54 no.2
• /
• pp.173-179
• /
• 2016

Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.8 no.8
• /
• pp.1760-1768
• /
• 2004

A P2P is a method that can share and exchange on digital resources through a direct connection on personnel without a central server. In this paper, we apply a P2P technology to a traditional electronic commerce method so that a seller and a customer can perform a commercial transaction through a direct communication. As such a result, we propose a new e-Commerce model on P2P environment to assure fairness in commercial transactions. To achieve our goal, we put an escrow server which is responsible for guarantee fair contents delivery and payment for the contents between a seller and a customer. When a customer buys content, he first obtains reputation values implicating reliability for the content and refers to these values to determine purchase. The proposed scheme, we can improve the reliability for the purchasing content and provide the fairness to both a seller and a customer simultaneously.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.38A no.3
• /
• pp.268-276
• /
• 2013

In this paper, we introduce the technologies to handle the interference in the heterogeneous network and evaluate the performance of enhanced Inter-Cell Interference Coordination (enhanced ICIC, eICIC) techniques that are being introduced in 3GPP Release 10. In the time-domain eICIC scheme, time-domain resources are scheduled to avoid the interference by using Almost Blank Subframe (ABS) and Cell Range Expansion (CRE). To mitigate the cross-tier interference between macro and femtocell, it is important to efficiently combine the ABS and CRE in heterogeneous network. Since it is hard to evaluate the total throughput of heterogeneous network numerically, we evaluate the total throughput by using system level simulation (SLS). As a result of evaluation, the throughputs of many different cases of combination of ABS and CRE are compared.