• Journal of Microbiology and Biotechnology
• /
• 제11권2호
• /
• pp.281-286
• /
• 2001

Since the number of amino groups which are exposed by deacetylation of acetyl-D-glucosamine influences antimicrobial activity, a chitosan oligosaccharide (COS) derivative by N-conjugation of COS with asparagine, an amino acid with two amino groups, was synthesized and the antimicrobial effect on E. coli growth was compared with other COS derivatives which were N-conjugated with glycine, alanine, aspartic acid, cysteins, an methionine, and unmodified COS. The structure of asparagine N-conjugated COS (Asn-COS) derivative was identified by using a FT-IR, $^{13}C\;FT-NMR$, and an elemental analyzer. The antimicrobial activity of Asn-COS against E. coli growth was significantly improved as compared to the other COS derivatives as well as COS itself. This means that Asn-COS with two positive charges strongly interacts with the carboxyl negative charges on the bacteria cell wall. The results for Asn-COS were as follows: 100% bactericidal activity, 0.002% MIC, and no growth of E. coli during 3 days of culture time, suggesting that Asn-COS may be useful as a new antibiotic agent.

• Journal of KIISE:Computing Practices and Letters
• /
• 제16권7호
• /
• pp.759-767
• /
• 2010

RT-eCos 3.0 is a micro-sized embedded real-time kernel that has been developed based on the open source eCos 3.0 to support the basic task model of the well-known distributed real-time object model, TMO(Time-Triggered Message-triggered Object). In this paper, the design and implementation techniques of the RMMC(Real-time Multicast & Memory replication Channel) that is a standard distributed IPC model of the TMO is described based on the RT-eCos 3.0. And the support technique of the global time for using the same time in a distributed environment using the RMMC is also described. The developed global time based RMMC supports highly abstracted distributed IPC environment in a wide area distributed computing environment with the RT-eCos 3.0.

• KIPS Transactions on Computer and Communication Systems
• /
• 제12권4호
• /
• pp.127-134
• /
• 2023

This paper describes how to port the eCos operating system and PROFINET communication stack developed based on gcc to the IAR compiler. The eCos operating system provides basic functions such as multi-thread, TCP/IP, and device driver for PROFINET operation, so there is no need to change it when developing PROFINET applications. Therefore, in this study, we reuse an eCos library built with gcc and it link with PROFINET communication stack that are ported to IAR complier. Due to the different of the gcc and IAR linker, symbol definitions and address of the constructors should be changed using the external tool that generates symbol definitions and address of the constructors from MAP file. In order to verify the proposed method, it was confirmed that the actual I/O was operating normally through PROFINET IRT communication by connecting to the Siemens PLC. IAR compiler has better performance in both the compile time and the size of the generated binary. The proposed method in this study is expected to help port various open sources as well as eCos and PROFINET communication stacks to other compilers.

• Journal of Korean Ophthalmic Optics Society
• /
• 제5권1호
• /
• pp.43-48
• /
• 2000

It was to adjust the luminance of light by the rotation angle of the polarizes and analyzer. The luminance value Lmax, Lmin of Contrast Sensitivity could be obtained from the rotation angle ${\theta}_m$ of the average luminance($L_m$), the rotation angle(${\theta}_{max}$, ${\theta}_{min}$) of the maximum and the minimum's amplitude. $$L_{max}=I(0)e^{-2at}{\cdot}cos^2{\theta}_m(1+C_s^{-1})$$ $$L_{min}=I(0)e^{-2at}{\cdot}cos^2{\theta}_m(1-C_s^{-1})$$ We obtained the rotation angle(${\theta}_{max}$, ${\theta}_{min}$) of the polarizes and analyzer from the rotation angle ${\theta}_m$ of the average luminance($L_m$) and the Contrast Sensitivity($C_s$). $${\theta}_{max}=cos^{-1}[cos{\theta}_m{\cdot}(1+C_s^{-1})^{1/2}]$$ $${\theta}_{min}=cos^{-1}[cos{\theta}_m{\cdot}(1-C_s^{-1})^{1/2}]$$.

• The Journal of the Korea Contents Association
• /
• 제13권4호
• /
• pp.44-52
• /
• 2013

The SIFT algorithm is being actively researched for various image processing applications including video surveillance and autonomous vehicle navigation. The computation of sin/cos function is the most cost part needed in whole computational complexity and time for SIFT descriptor. In this paper, we implement a hardware to sin/cos function of descriptor on sift feature detection algorithm. The proposed Sin/Cosine processor is coded in Verilog and synthesized and simulated using Xilinx ISE 9.2i. The processor is mapped onto the device Spartan 2E (XC2S200E-PQ208-6). It consumes 149 slices, 233 LUTs and attains a maximum operation frequency of 60.01 MHz. As compared with the software realization, our FPGA circuit can achieve the speed improvement by 40 times in average.

• Proceedings of the Korean Information Science Society Conference
• /
• 한국정보과학회 2011년도 한국컴퓨터종합학술대회논문집 Vol.38 No.1(B)
• /
• pp.395-397
• /
• 2011

RT-eCos3.0은 대표적 분산 실시간 객체 모델인 TMO(Time-triggered Message-triggered Object)의 실행을 제공하기 위하여 공개소스 eCos3.0 기반으로 개발된 초경량 경성 실시간 임베디드 운영체제이다. RT-eCos3.0에서는 그간 스레드의 최장 수행 시간 입력이 필요 없는 EDF 및 FIFO 스케줄러를 지원하여 왔다. 본 논문에서는 TMO의 시간 구동 스레드와 메시지 구동 스레드의 스레드 등록 시 최장 수행 시간을 입력 받아 이를 기반으로 마감시간까지의 수행시간 대비 잔여시간을 이용하는 LLF (Least Laxity First) 스케줄러를 클럭 인터럽트 핸들러 내에 구현하고 각 스레드로 하여금 스케줄링 정책을 선택할 수 있도록 구현하였다.

• Journal of KIISE:Computing Practices and Letters
• /
• 제15권4호
• /
• pp.304-308
• /
• 2009

This paper presents the deadline driven CPU-Power management scheme for the Real-Time Embedded OS: named TMO-eCos. It used the scheduling scenarios generated by a task serialization technique for hard real- time TMO system. The serializer does a off-line analysis at design time with period, deadline and WCET of periodic tasks. Finally, TMO-eCos kernel controls the CPU speed to save the power consumption under the condition that periodic tasks do not violate deadlines. As a result, the system shows a reasonable amount of power saving. This paper presents all of these processes and test results.

• Proceedings of the Korea Information Processing Society Conference
• /
• 한국정보처리학회 2013년도 춘계학술발표대회
• /
• pp.37-40
• /
• 2013

RT-eCos 3.0은 대표적 분산 실시간 객체 모델인 TMO(Time-triggered Message-triggered Object)의 기본적 태스크 모델 실행을 위하여 eCos 3.0 기반으로 개발된 초경량 경성 실시간 임베디드 운영체제이다. 본 논문에서는 RT-eCos 3.0 기반의 다중센서 데이터 처리를 위한 모듈의 설계 및 구현에 대해 기술하며, 실시간 다중센서 데이터 처리를 위한 스케줄링 사전분석 기술에 대해서도 기술한다.

• Journal of the Korean Society of Food Science and Nutrition
• /
• 제33권2호
• /
• pp.236-243
• /
• 2004

Antimicrobial effect of chitosan and its oligosaccharides was examined on Vibrios sp., Edwardsiella tarda and Streptococcus sp., which are major pathogenic bacteria inducing bacterial diseases of acquacultured flounder. Chitosan oligosaccharides (COS ) were produced by enzymatic hydrolysis of chitosan in an ultrafiltration mombrane bioreactor system which was established with three membranes with different molecular weight cut-off (MWCO) 1,000, 5,000 and 10,000, and fractionated into three kinds of COS, based on their molecular weight sizes. The three kinds of COS were as follows : relatively high molecular weight COS [HMW-COS, molecular weight distribution of 7,000 to 24,000 Da〕, medium molecular weight COS 〔MMW-COS, 1,500 to 6,000 Da〕, and low molecular weight COS 〔LMW-COS, 1,000 to 1,500 Da). Chitosan and HMW-COS effectively inhibited the growths of Vibrio sp. and Streptococcus sp. and their antimicrobial activities were superior to the others with smaller molecular weights. This result suggested that antimicrobial effect of chitosan preparations extremely depend on their molecular weight sizes. Antimicrobial effect of chitosan and HMW-COS on E. tarda was improved by longer inoculation times. Scanning electron microscopy in morphological change of E. tarda treated with chitosan preparations showed that chitosan and HMW-COS bound to the cells and suppressed the growth of the cells. This observation appears to prove the fact that positive charged amines of chitosan electrostatically bind to negative charged compounds of cell walls.

• Journal of Microbiology and Biotechnology
• /
• 제11권2호
• /
• pp.234-241
• /
• 2001

The C-terminal 393 bp region of the human interleukin-16 (IL-16) gene was cloned and expressed in E. coli along with mammalian cell lines. Recombinant IL-16 expressed from E. coli was 22 kDa on SDS-PAGE and showed 260% of chemoattractant activity at a concentration of $0.1\;{\mu}g/ml$. HeLa, COS, and Neuro-2a cells were transduced by recombinant retrovirus vector pLNC/IL-16/IRES/TK and the intracellular and secreted amounts of IL-16 produced by HeLa/IL-16/TK, COS/IL-16/TK, and Neuro-2a/IL-16/TK cells were determined by enzyme-linked immunosorbent assay (ELISA). HeLa/IL-16/TK $(1{\times}10^5)$ and COS/IL-16/TK $(1{\times}10^5)$ cells secreted 36.1 and 13.3 ng of IL-16 for 48 h, respectively. Forty-nine ng and 86.4 ng of IL-16 remained in the cell lysates of HeLa/IL-16/TK and COS/IL-16/TK. Intracellular and secreted amounts of IL-16 from Neuro-2a/IL-16/TK $(5{\times}10^5)$ cells during 24 h cultivation were 50 ng and 3.3 ng, respectively. Also, HeLa and COS cells wee stably transfected with mammalian expression vector pCRIII/IL-16. Both culture media and cell lysates prepared from HeLa/IL-16 cells and COS/IL-16 cells showed chemoattractant activity ranging from 190% to 460% as compared to the control experiment. Expression of the herpes simplex virus thymidine kinase (HSV0tk) gene in pLNC/IL-16/ IRES/TK bicistronic retroviral expression vector was verified by performing a genciclovir (GCV) sensitivity assay. Finally, IL-16 repressed Tat-transactivated human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR) promoter activity.