• Molecules and Cells
• /
• 제42권2호
• /
• pp.123-134
• /
• 2019

Lysophosphatidic acid (LPA) is an endogenous lysophospholipid with signaling properties outside of the cell and it signals through specific G protein-coupled receptors, known as $LPA_{1-6}$. For one of its receptors, $LPA_1$ (gene name Lpar1), details on the cis-acting elements for transcriptional control have not been defined. Using 5'RACE analysis, we report the identification of an alternative transcription start site of mouse Lpar1 and characterize approximately 3,500 bp of non-coding flanking sequence 5' of mouse Lpar1 gene for promoter activity. Transient transfection of cells derived from mouse neocortical neuroblasts with constructs from the 5' regions of mouse Lpar1 gene revealed the region between -248 to +225 serving as the basal promoter for Lpar1. This region also lacks a TATA box. For the region between -761 to -248, a negative regulatory element affected the basal expression of Lpar1. This region has three E-box sequences and mutagenesis of these E-boxes, followed by transient expression, demonstrated that two of the E-boxes act as negative modulators of Lpar1. One of these E-box sequences bound the HeLa E-box binding protein (HEB), and modulation of HEB levels in the transfected cells regulated the transcription of the reporter gene. Based on our data, we propose that HEB may be required for a proper regulation of Lpar1 expression in the embryonic neocortical neuroblast cells and to affect its function in both normal brain development and disease settings.

• 한국지구물리탐사학회:학술대회논문집
• /
• 한국지구물리탐사학회 2009년도 특별 심포지엄
• /
• pp.57-65
• /
• 2009

Selection of good mineralized area is a combination of the integration of all the available geo-scientific (i.e., geological, geochemical, and geophysical) information, extrapolation of likely features from known mineralized terrenes and the ability to be predictive. The time-space relationships of the hydrothermal deposits in the East Asia are closely related to the changing plate motions. Also, two distinctive hydrothermal systems during Mesozoic occurred in Korea: the Jurassic/Early Cretaceous deep-level ones during the Daebo orogeny and the Late Cretaceous/Tertiary shallow geothermal ones during the Bulguksa event. Both the Mesozoic geothermal system and the mineralization document a close spatial and temporal relationship with syn- to post-tectonic magmatism. The Jurassic mineral deposits were formed at the relatively high temperature and deep-crustal level from the mineralizing fluids characterized by the relatively homogeneous and similar ranges of ${\delta}^{18}O$ values, suggesting that ore-forming fluids were principally derived from spatially associated Jurassic granitoid and related pegmatite. Most of the Jurassic auriferous deposits (ca. 165-145 Ma) show fluid characteristics typical of an orogenic-type gold deposits, and were probably generated in a compressional to transpressional regime caused by an orthogonal to oblique convergence of the Izanagi Plate into the East Asian continental margin. On the other hand, Late Cretaceous ferroalloy, base-metal and precious-metal deposits in the Taebaeksan, Okcheon and Gyeongsang basins occurred as vein, replacement, breccia-pipe, porphyry-style and skarn deposits. Diverse mineralization styles represent a spatial and temporal distinction between the proximal environment of sub-volcanic activity and the distal to transitional condition derived from volcanic environments. However, Cu (-Au) or Fe-Mo-W deposits are proximal to a magmatic source, whereas polymetallic or precious-metal deposits are more distal to transitional. Strike-slip faults and caldera-related fractures together with sub-volcanic activity are associated with major faults reactivated by a northward (oblique) to northwestward (orthogonal) convergence, and have played an important role in the formation of the Cretaceous Au-Ag lode deposits (ca. 110-45 Ma) under a continental arc setting. The temporal and spatial distinctions between the two typical Mesozoic deposit styles in Korea reflect a different thermal episodes (i.e., late orogenic and post-orogenic) and ore-forming fluids related to different depths of emplacement of magma (i.e., plutonic and sub-volcanic) due to regional changes in tectonic settings.

• 식물분류학회지
• /
• 제43권1호
• /
• pp.56-62
• /
• 2013

경남 마산에서 한반도 미기록 벼과식물인 Echinochloa oryzoides (Ard.) Fritsch를 발견하였다. 본 종은 논피와 마찬가지로 주로 논에서 발견되어 출수전에는 형태적으로 벼와 비슷하여 구별이 어려우나 원추화서는 아래로 처지고 소수가 논피보다 드믈게 달리며 제1포영은 소수의 1/4-2/5 정도이고 일정하게 1 cm 이상의 긴 까락을 가지고 있다. 염색체 수는 2n = 54이다. 국명은 식물체가 논피와 비슷하고 논에서 발견되는 점을 고려하여 '나도논피'로 신칭하였다. 근연종인 논피는 원추화서가 직립하고 제1포영은 소수의 반 이상이며 제1 호영은 까락이 없거나 짧은 까락이 불규칙하게 달린 점이 나도논피와 구별된다. 염색체 수는 2n = 36이다. 논피의 학명은 E. oryzicola (Vasinger) Vasinger로 정정하였다.

• Journal of Microbiology and Biotechnology
• /
• 제19권12호
• /
• pp.1576-1581
• /
• 2009

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has led to an urgent need for the discovery and development of new antibacterial agents. As part of an ongoing investigation into the antibacterial properties of natural products, 20-hydroxyecdysone (20E), isolated from the roots of Achyranthes japonica Nakai, was found to be active against MRSA, either alone or in combination with ampicillin (AM) or gentamicin (GM), via checkerboard assay. This study investigated the antibacterial activity of 20E, which exhibited poor antibacterial activity ($MIC=250-500\;{\mu}g/ml$) against MRSA tested. The combined activity of AM or GE plus 20E against MRSA resulted in fractional inhibitory concentractions (FICs) ranging from 4.00 to $0.031\;{\mu}g/ml$, respectively. Meanwhile, the FIC index ranged from 0.16-4.50, indicating a marked synergistic relationship between AM, GE, and 20E against MRSA. Time-kill assays also showed a remarkable decrease between the combination and the more active compound. Therefore, this study demonstrated that AM, GE, and 20E can act synergistically in inhibiting MRSA in vitro.

• 천문학회보
• /
• 제42권1호
• /
• pp.30.2-31
• /
• 2017

Compact elliptical (cE) galaxies are in a rare class of stellar systems characterized by high stellar densities, small sizes, high velocity dispersion, and high metallicity corresponding to elliptical galaxies. cE galaxies have been observed around massive galaxies, so they could be formed under strong influences of tidal stripping and truncation. However, the recent discovery of isolated cE galaxies requires the need of new formation scenarios. We aim at finding cE galaxies in various environments using SDSS DR12, and studying stellar population of cEs as function of environments. Based on the typical properties of cE galaxies, we selected cE candidates by restricting that low-luminosity Mg > 19.5 mag, small sizes Re < 700 pc, and high velocity dispersions ${\sigma}$ > $60kms^{-1}$. Since effect radii of cE candidates are mostly smaller than the seeing size of SDSS photometry, we calculated the effective radius by fitting a Sersic profile. In addition, we assumed that host galaxies have brightness with Mr < -21 mag, and an environmental parameter is computed as distances between cE galaxies and host-galaxies. We found 112 cE galaxies at z < 0.05, which have high sersic indices (mean value is 5.2) similar to the typical massive elliptical galaxies. Mgb values of cE galaxies increase as the distances from the host galaxies decrease. Especially, for cEs close to the host galaxies (NcE; $D_{host}$ < 300 pc), the Mgb values are similar to those of massive elliptical galaxies, which is consistent with the previous studies. On the other hand, cE galaxies distant from the host galaxies (DcE; Dhost >300 pc) have lower Mgb values than the conventional cE. The Mgb values follow the ${\sigma}$-Mgb relation of elliptical galaxies, and are connected to its faint end. This can be explained as a result of different merger histories for differing environments. For example, NcE galaxies are formed by tidal stripping by massive galaxies as suggested by previous studies, but DcE galaxies could be linked with high-redshift spheroids (e.g. red nuggets) which have not evolved into present-day elliptical galaxies because of the environmental influences.

• Journal of Microbiology and Biotechnology
• /
• 제18권4호
• /
• pp.648-657
• /
• 2008

In this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in $FB_1$ biosynthesis.

• 한국콘텐츠학회논문지
• /
• 제9권2호
• /
• pp.326-338
• /
• 2009

유비쿼터스 비즈니스 환경은 기존의 e-비즈니스, m-비즈니스와는 다른 특성을 가지고 있다. 이는 u-비즈니스 모델을 개발할 때 유비쿼터스 컴퓨팅 기술의 특성과 유비쿼터스 비즈니스 환경을 고려해야 한다는 것을 의미한다. 본 논문에서는 유비쿼터스 비즈니스 패러다임의 핵심인 가치의 공동 창출 경험에 초점을 맞춘 u-비즈니스 모델 개발 프레임워크를 제시했다. 본 논문의 u-비즈니스 모델 개발 프레임워크는 8단계로 구성되어 있고 각 단계별 세부 절차를 구체적으로 제시했다. 새로운 비즈니스 패러다임 하에서 성공적인 비즈니스 모델을 발굴하고 수행하고자 하는 기업들에게 본 논문에서 제안한 u-비즈니스 모델 개발 프레임워크는 유용한 가이드라인을 제공할 수 있을 것으로 기대된다.

• 한국정보과학회논문지:정보통신
• /
• 제31권5호
• /
• pp.511-518
• /
• 2004

DCOM, CORBA 등의 분산객체(distribute object)의 뒤를 이어 등장한 웹 서비스(Web Services) 기술은 SOAP, WSDL, UDDI 둥의 XML 기반의 공개 표준을 바탕으로 하여 웹을 통한 원격 프로시저 호출(Remote Procedure call)의 메커니즘을 제공하여, e-비즈니스에서 통합과 협업의 방법으로 최근 각광을 받고 있다. 특히, UDDI는 웹 서비스를 등록 및 검색을 가능하게 해주는 레지스트리(registry)로, 웹 서비스를 위한 인프라를 제공하는 역할을 담당하고 있다. 하지만 기존 UDDI는 검색 방법이 단순하고, 해당 웹 서비스의 품질을 평가하여 검색할 수 있는 방법을 제공하고 있지 못하다는 문제를 가지고 있다. 본 논문은 웹 서비스의 품질을 평가하여 그에 대한 검색을 가능하게 해줄 수 있는 UDDI 개선 모델을 제안하고자 한다.

• 천문학회보
• /
• 제41권2호
• /
• pp.39.1-39.1
• /
• 2016

We report the discovery of a kiloparsec-size supershell in the outskirts of the Milky Way with the compact high-velocity cloud, HVC 040+01-282 (hereafter, CHVC040), at its geometrical center using the "Inner-Galaxy Arecibo L-band Feed Array" HI 21 cm survey data. Supershells are large gaseous shells, which could be produced by one of most energetic activities with an explosion energy more than $3{\times}1052erg$. The most promising origin is the explosion of multiple supernovae in OB associations, or alternatively, the impact of HVCs falling into the Galactic disk. We found the association between CHVC040 and the Galactic supershell by analysis of their morphological and physical properties. Our results imply that some compact HVCs can survive their trip through the Galactic halo and inject energy and momentum into the Milky Way disk.

• BMB Reports
• /
• 제39권6호
• /
• pp.788-793
• /
• 2006

Bacterial DNA containing immunostimulatory CpG motifs can stimulate antigen-presenting cells to express co-stimulatory molecules and to produce various cytokines in vivo and in vitro. In this study, we fragmented macromolecular E.coli genomic DNA with DNase I, and analyzed the ability of the resulting DNA fragments to induce the NF-${\kappa}B$ activation and humoral immune response. Furthermore, using computational analysis and luciferase assay for synthetic ODNs based on the sequence of the immunostimulatory DNA fragments (DF-ODNs), an active component of DF-ODNs sequences was investigated. Experimental results demonstrated that DF-ODN is optimal for the NF-${\kappa}B$-responsive promoter activation in the mouse macrophage cell line and the humoral immune response in vivo. In agreement with the activity of the DF-ODNs processed by DNase I, a synthetic ODN based on the DF-ODN sequences is potent at inducing IL-12 mRNA expression in primary dendritic cells. These results suggest that the discovery and characterization of a highly active natural CpG-ODN may be achieved by the analyses of bacterial DNA fragments generated by a nuclease activity.