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Inhibitory Effects of the Extracts from Eleutherococcus senticosus Maxim. on Histamine-release from Rat's Mast Cell

  • Jeong, Jae-Hun;Kim, Young-Seon;Baek, Seung-Hwa;Park, Kwang-Hyun
    • Korean Journal of Plant Resources
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    • v.24 no.3
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    • pp.324-329
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    • 2011
  • Eleutherococcus senticosus Maxim. has been successfully used as an oriental medicine for various diseases including allergic disorders. Histamine is a major factor on various allergic responses and it is reported that histamine was released from mast cells by sensitization of allergens. In this study, ethanol extracts from E. senticosus Maxim. were prepared and the composition was analyzed by high performance liquid chromatography. The eleutheroside B as a primary effective component of E. senticosus was contained approximately 225 mg/kg in root bark extracts. The extracts were found to significantly inhibit compound 48/80-induced histamine release form mast cells in dose dependent manner. However the extracts had low cytotoxicity on the mast cells with MTT assay. These results showed that E. senticosus Maxim. extracts may be the effective materials on inflammatory disorders.

Production of milk-originated antimicrobial peptide, lactoferricin, in E. coli (미생물을 이용한 우유 유래 항균펩타이드(락토페리신)의 생산)

  • Kang, Dae-Kyung
    • 한국유가공학회:학술대회논문집
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    • 2007.09a
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    • pp.13-20
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    • 2007
  • Bovine lactoferricin(LFcin B) is a peptide of 25 amino acids that originated from the N terminus of bovine lactoferrin, and is characterized as having potent antimicrobial activity against bacteria, fungi, protozoa and viruses. But, direct expression of Lfcin B is lethal to Escherichia coli. For the efficient production of Lfcin B in E. coli, we developed an expression system in which the gene for cationic Lfcin B was fused to an anionic peptide gene, and successfully expressed the concatemeric fusion gene in E. coli. The purified recombinant Lfcin B was found to have antimicrobial activity, as the native Lfcin B peptide does.

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Verification Study for Remotely Sensed Soil Moisture (인공위성 토양수분 자료 검증에 관한 연구)

  • Hur, Yoo-Mi;Choi, Min-Ha;Jung, Sung-Won
    • Proceedings of the Korea Water Resources Association Conference
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    • 2010.05a
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    • pp.1564-1569
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    • 2010
  • 토양수분은 수문현상 즉, 물의 순환과정을 이해하고 기상변화를 고려하는데 중요한 인자 중 하나이며 이는 최근 이상기후로 인한 가뭄 및 홍수 등의 자연재해가 우리나라 전역에 빈번히 발생되고 있는 가운데 이러한 현상을 보다 정확히 해석하기 위해 토양수분의 중요성이 더욱 부각되고 있다. 현재 이를 관측 및 분석하고 있으나 대부분 관측기간이 짧고 장비가 노후화되어 많은 결측치를 나타내고 있으며 관측치가 있더라도 여러 가지 요인으로 인해 관측에 대한 분석의 신뢰도가 떨어진다. 이로 인하여 본 연구에서는 광역적 범위에서 정확한 토양수분량 측정을 하고 있는 Advanced Microwave Scanning Radiometer E (AMSR-E) 위성관측 데이터를 기존의 토양수분 자료와 비교/검증하여 이의 활용방안을 모색하고자 한다.

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Characteristics of Water Levels and Occurrences of Thermal Groundwater at the Yuseong Spa Area (유성지구 지열수자원의 산출 및 수위변동 특성)

  • Moon, Sang-Ho;Ha, Kyoo-Chul;Kim, Yung-Sik;Cho, Sung-Hyeon
    • Economic and Environmental Geology
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    • v.39 no.5 s.180
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    • pp.537-554
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    • 2006
  • Water levels of thermal groundwater ($>30^{\circ}C$) were recorded from March 2002 to June 2006 at several monitoring wells within the Yuseong spa area. Using these data, we elucidated the long-term cyclic fluctuations of thermal groundwater levels with 1 year period. We also observed a noticeable water level variation with periods of 0.5, 1 and 7 days in most monitoring wells, which indicates relatively good hydraulic connectivity within the main hotspring area. By comparing water level variations among several wells, we found out that E-W and N-S trending geological structures should be an important control factor for emplacement and flow of thermal groundwater in the study area. It may be also inferred that geothermal source is highly associated with the hydraulic connectivity of aquifers at the Yuseong spa area.

Age and growth of the black rockfish, Sebastes inermis, in the Jeonnam marine ranching area in the southern Sea of Korea (한국 남해 전남바다목장해역 볼락, Sebastes inermis의 연령과 성장)

  • Kim, Hee-Yong;Kim, Sang-Hwa;Huh, Sun-Jung;Seo, Young-Il;Lee, Sun-Gil;Ko, Jun-Chul;Cha, Hyung-Kee;Choi, Mun-Sung
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.46 no.4
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    • pp.346-357
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    • 2010
  • Characteristics of age and growth of the black rockfish, Sebastes inermis sampled in the Jeonnam Marine Ranching Area (JMRA) around Geumo Islands south of Yeosu were investigated using 642 otoliths from March 2009 to February 2010. The opaque zone was formed in September once a year and hence it was used as an annulus. The parturient period was December to January, and therefore the duration from fertilization to the complete formation of the opaque zone was nineteen months. From the parameters calculated using the average length when the year ring was formed, growth of S. inermis were expressed by von Bertalanffy growth equation as $L_t$=23.267 ($1-e^{-0.4406(t+1.1971)}$) for females and $L_t$=22.030 ($1-e^{-0.5312(t+0.6834)}$) for males when is total length in age t. Through the growth equations, the maximum length was determined as 23.27cm for females and 22.03cm for males and the growth factor as 0.4406/yr and 0.5312/yr, respectively. Finally, the growth of female S. inermis is larger than the one of male S. inermis.

Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry

  • Park C. S.;Li Z. H.;Sung N. D.;Jin D. I.;Cong P. Q.;Kim E. S.;Song E. S.;Yi Y. J.
    • Reproductive and Developmental Biology
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    • v.29 no.4
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    • pp.253-257
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    • 2005
  • This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than $80\%$ of fresh sperm washed with mTLP-PVA medium at $20^{\circ}C$ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane $(36.4\~46.9\%)$ and nonfunctional mitochondrion $(55.1\~71.1\%)$ in the mTLP-PVA and BTS washing media at $20^{\circ}C$. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at $4^{\circ}C$ washing temperature than at $20^{\circ}C$ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

The Survey on Froth Floatation of Low Grade Copper Ore from the Australia (호주산 저품위 동광의 부유선별에 관한 특성조사)

  • Kim, Joon Soo;Kim, Woo Jin;Hwang, Ha;Kim, Myong Jun;Kim, Wantae
    • Resources Recycling
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    • v.25 no.6
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    • pp.98-104
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    • 2016
  • The purpose of this study is to obtain an optimum condition of the concentrate by floatation using Australian low grade copper ore of suitable particle size by crushing, grinding and sizing. The effect of the dosage of collector, activator, depressant and change of acidity on the floatation was investigated. The floatation conditions comprising of pH 9.0 acidity, 500g/t $CuSO_4{\cdot}5H_2O$ activator, 500g/t K.E.X. collector, 1500g/t $Na_2SO_3$ depressant and 25wt.% plup density were optimized. Grade and recovery of copper in the concentrate obtained by froth floatation under optimum conditions were 4wt.% and 65wt.% respectively.

Floristic Study of Yeoseo-do (Isl.) in Korea (여서도의 관속식물상)

  • Kim, Sun-Yu;Yun, Jong-Hak;Kim, Jin-Seok;Kim, Jung-Hyun
    • Korean Journal of Plant Resources
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    • v.27 no.2
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    • pp.133-154
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    • 2014
  • We investigated the flora of Yeoseo-do (N $33^{\circ}58^{\prime}$, E $126^{\circ}55^{\prime}$), located in Wando-gun, Jeollanam-do, South Korea and discussed the vascular plants found there. Four separate field trips were completed from April to October of 2013. The vascular plants were summarized as 463 taxa; 112 families, 306 genera, 409 species, 6 subspecies, 42 varieties, 5 forms and 1 hybrids. The number of endemic species of Korea were 6 taxa, and 12 taxa designated by the Ministry of Environment as red data of endangered vascular plants were investigated in this region. There were a total of 98 taxa of floristic regional indicator plants and one taxon of level I endangered species, Neofinetia falcata (Thunb.) Hu, as designated by the Ministry of Environment. 33 taxa have shown their southern distributional limit ranges, and 10 taxa of halophytes were confirmed in the Yeoseo-do (Isl.). In additon, 27 taxa of the naturalized plants were recorded.

The Influences of Family Resources on Caregiving Stress for Dementia Elderly in Homemakers (치매노인 부양주부의 가정자원이 부양스트레스에 미치는 영향)

  • 장윤옥;정서린
    • Journal of the Korean Home Economics Association
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    • v.42 no.6
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    • pp.73-88
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    • 2004
  • The purpose of this study was to explore the influences of family resources, i.e. objective resources, perceived adequacy of resources, locus of control, social support, family cohesion, and family adaptability on caregiving stress for dementia elderly in homemakers. The subjects of this study were 141 married women who had cared for dementia elderly. The survey method was a questionnaire. And the data was analyzed by means of Cronbach's, factor analysis, and multiple regression. The main findings were as follows: First, Homemakers' caregiving stress was positively correlated to their academic background and monthly income, but negatively correlated to perceived adequacy of human resources, perceived adequacy of material resource, psychical support resource from relatives, and internal locus of control. Second, caregiving stress of homemakers was affected by family cohesion, monthly income, psychical support resource from relatives, perceived adequacy of human resources, and external locus of control.