• International Journal of Advanced Culture Technology
• /
• 제4권4호
• /
• pp.70-74
• /
• 2016

Electronic commerce (E-commerce) using virtual reality (VR) has the advantage of being able to purchase products without restrictions of time and location by overcoming the limitations of existing offline transactions. It is still a rudimentary but fast growing technology, and the use of E-commerce in VR is expanding. The barriers that consumers might face in utilizing E-commerce in VR is the relevance to Information and Communications Technologies (ICT) technology. Fundamentally, it requires Internet access and use through PCs or mobile devices such as smart phones. Because unlike off-line markets, it is difficult to determine the purchase patterns of customers, customer purchasing behavior analysis must be done using computer access records. In order to expand and develop E-commerce in VR in the future, learning ability should be improved through combining with artificial neural network by deep learning that is recently in the spotlight, and the ability to overcome errors need to be improved to enable use in various fields.

• 한국컴퓨터정보학회:학술대회논문집
• /
• 한국컴퓨터정보학회 2023년도 제67차 동계학술대회논문집 31권1호
• /
• pp.65-68
• /
• 2023

4차 산업혁명과 메타버스와 같이 빠르게 변화하는 정보화 시대에서 디지털 사각지대에 놓인 '디지털 소외 계층'들이 존재하고 있다. 특히 고령자 같은 경우엔 빠르게 변화된 사회 속에서 보장받아야 할 디지털 교육을 받지 못하여 이제는 당연하게 누려야 할 편리함까지 놓치고 있다. 본 논문에서는 이런 문제들을 해결하기 위한 효과적인 디지털 교육 방안으로 '놀이'와 '게임' 그리고 '컴퓨터'를 융합시킨 'e스포츠'를 통해 디지털 치료제로서 제안하고자 한다. 이는 단순한 '교육'이 아닌 새로운 실버 콘텐츠로 접근하여 노인들의 또 다른 문화로 개척하며 디지털 소외 문제를 해결하는 것과 동시에 고령자들에게 'e스포츠'의 발전과 인식개선에도 기대해 본다.

• KSBB Journal
• /
• 제23권3호
• /
• pp.187-192
• /
• 2008

Lysozyme-producing microorganisms were isolated to obtain bacteria which can efficiently solubilize microbial cells. Cells of normal and chloroform-treated Escherichia coli and Micrococcus Iysodeikticus were used as model substrates to isolate lysozyme-producing microorganisms and investigate the efficiency of cell lysis. The culture supernatant of the isolate New1 (98% similarity of 16S rDNA sequence with Thermomonas haemolytica) showed different lytic characteristics for different substrates. Thermal treatment (autoclave) of substrate cells showed a significant effect on cell solubilization by culture supernatant of the New1. For autoclaved substrate cells, E. coli, M. Iysodeikticus and chloroform-treated E. coli were solubilized by 58.7%, 49.4% and 79.1%, respectively, in the culture supernatant of New1. The lytic activity of New1 was mainly caused by lysozyme produced by the isolate. It was also showed that New1 exhibited high protease activity and a little cellulase activity.

• Asia pacific journal of information systems
• /
• 제32권3호
• /
• pp.656-683
• /
• 2022

The COVID-19 pandemic has profoundly affected the world, inflicting nationwide lockdowns interrupting conventional schooling through schools, colleges and universities. Educational institutions are struggling to maintain learning continuity through remote learning solutions. Still, the students' perception of this 'new normal' mode and pace of learning needs to be examined to ensure the success of these efforts. This study aimed at examining the perception of higher education students in India and Croatia especially with respect to the association between cultural orientation and the e-learning. The period considered for the data collection was from March 2020 to September 2020. Correspondence analysis was attempted to create spatial maps to depict the respondent choices. Students from both the regions agreed to the high-power distance that existed in their cultures and considered the role of device and content to be an important dimension of e-learning for it to be effective, but the results also pointed out some differences in their choices on other culture dimensions as well as factors affecting e-learning which make this study unique and suggest in-depth future research for conclusive results.

• KSBB Journal
• /
• 제21권4호
• /
• pp.298-301
• /
• 2006

트레할로스 합성효소(trehalose synthase)의 효율적인 생산을 위하여, 5 종류의 plasmid를 형질전환 시킨 재조합 E. coli를 이용하여 균체생산량과 효소발현량을 비교하였다. Trehalose synthase의 활성은 fusion partner를 이용한 system 에서는 활성이 나타나지 않았으며, IPTG 유도 발현 시스템보다 항시적 발현 시스템을 사용하는 E. coli K12/pHCETS에서 가장 높은 활성을 나타내었다. 선별된 재조합 E. coli K12/pHCETS를 사용하여 회분식 및 유가배양을 수행하였으며, 유가식 배양의 경우 균체논도는 20 g/L, 최종 trehalose synthase 활성은 13.7 U/ml을 나타내었다. 이러한 결과는 트레할로스 생산을 위한 trehalose synthase가 재조합 E. coli의 발효에 의해 경제적으로 생산되어질 수 있다는 가능성을 보여 주었다.

• International Journal of Advanced Culture Technology
• /
• 제11권2호
• /
• pp.125-131
• /
• 2023

OVO is a digital platform that provides simple payments and smart financial services, as well as one of the largest digital payment platforms in Indonesia. It has wide coverage and security when making payments, and supports multiple settlement currencies. The purpose of this study is to explore the history, business model, and future strategic direction of OVO, an Indonesian e-wallet. To date, OVO has built its own mobile payment ecosystem covering a wide range of consumer scenarios including e-commerce, travel, offline shopping and finance. And it supports mobile banking, online banking, debit cards or selected partner merchants. Its three largest transaction categories are in the transportation, retail and e-commerce sectors. With over 110 million consumers and 1.3 million merchant users, it is one of the dominant e-wallets in Indonesian market and has become the country's e-payment market leader. OVO eWallet's 'One Card' model offers convenience and choice for users, thus contributing to the rapid growth of OVO eWallet. And OVO eWallet competes fiercely with other competitors, but OVO eWallet continues to grow in terms of the number of users and market share. Finally, this study analyzes the strategic goals and plans of OVO eWallet, predicts its future direction. OVO eWallet has a huge success, but there are still competition and challenges to face.

• 한국미생물·생명공학회지
• /
• 제32권3호
• /
• pp.243-248
• /
• 2004

산업적 R3HB의 생산을 위한 재조합 대장균의 pilot규모에서의 유가식 배양과 연속식 배양을 연구하였다. Pilot 규모에서의 R3HB생산을 위하여 안전한 two plasmid system pBRRed와 pMCS 105를 제작하였으며, 제작된 plasmids을 이용하여 여러 다른 대장균을 형질 전환하였다. 얻어진 재조합 대장균들을 30 l의 발효기에서 회분식 배양한 결과 대장균 XL-10 Gold(pBRRed, pMCS105)가 가장 높은 R3HB 농도를 보였다 30 1 발효기에서 대장균 XL-10 Gold (pBRRed, PMCS105)을 유가식 배양한 결과 22.4 g/1의 R3HB가 얻어졌으며, 생산성은 0.97 g/1-h를 보였다. 고농도의 R3HB를 고생산성으로 얻기 위하여 유가식 배양으로 높은 균체 농도를 얻은 후 연속 배양으로 R3HB를 생산하는 전략을 개발하였다. 그 결과 0.2 $h^{-1}$ 의 dilution rate에서 R3HB 생산성은 5.06 g/1-h를 보였다. 이러한 결과는 산업적 규모에서 재조합 대장균을 이용하여 R3HB를 고농도, 고생산성으로 얻을 수 있다는 것을 보여준다.

• BMB Reports
• /
• 제30권1호
• /
• pp.80-84
• /
• 1997

The synthesis and secretion of human angiogenin in E. coli by the natural leader sequence has been studied. We constructed a recombinant plasmid containing human angiogenin cDNA which encompassed all the coding region including leader sequence required for secretion. The recombinant plasmid was introduced into a suitable E. coli host. The angiogenin was detected in the culture medium and periplasm upon the induction of gene expression. The molecular weight of the secreted angiogenin was identical to that of authentic angiogenin purfied from human plasma when estimated by SDS-PAGE and immunoblotting. showing that the natural leader sequence was recognized and processed by the secretion machinery of E. coli. The angiogenin concentration in the culture medium reached a maximum within 2 h when expressed at $37^{\circ}C$ with 0.02~2 mM IPTG. In contrast, the expression level increased gradually over time up to 11 h at $23^{\circ}C$ with 0.002~2 mM IPTG and at $37^{\circ}C$ with 0.002 mM IPTG.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회A
• /
• pp.1411-1414
• /
• 2008

It is reported that mechanical stimulation takes a role in improving cell growth in skeletal system. And various research groups have showed that developed bioreactor to stimulate cell-seeded and threedimensional scaffold. In this study, we designed a custom-made bioreactor capable of applying controlled compression to cell-seeded agarose gel. This device consisted of a circulation system and compression system. In circular system, culture chamber was sealed for prohibiting contamination and media solution was circulated by pump. In compression system, mechanical stimuli were controlled by LabVIEW software and mechanical transfer system. Cell-encapsulated agarose gels were cultured for up to 7 days. There were significant differences between the number of cells grown in dynamic cell culture and in static cell culture from 3 days to 7 days.

• 한국전자거래학회지
• /
• 제9권1호
• /
• pp.221-235
• /
• 2004

본 논문은 많은 기업들이 지식 공유를 위하여 성공적인 지식경영 시스템 도입을 추진하는 과정에서 조직문화 요소와 관계에 대한 연구를 목적으로 한다. 최근논문에서 언급된바와 같이 기업이 지식경영 시스템을 도입하는데 많은 문제점이 발견되고 있다는 것을 알 수 있다. 발견된 문제점 중 조직문화 요소에 대한 연구가 미흡하여 성공적인 지식경영 시스템 구현을 위한 장애가 되고 있다. 본 연구는 특정한 조직문화 요소가 지식경영 시스템 구현에 미치는 관계를 살펴보기 위해 OCP와 KMTP tool을 사용하여 26개의 미국 기업을 대상으로 설문조사를 한 결과를 기반으로 성공적인 지식경영 시스템 구현에 관계가 있는 기업 조직문화 요소를 연구 조사하였다.