• 분석과학
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• 제30권2호
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• pp.89-95
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• 2017

The thermodynamic functions for the binding of the peptide Substance P (SP) on the surface of lipid vesicles made of various types of lipids were obtained by using isothermal titration calorimetry. The reaction enthalpies measured from the experiments were -0.11 to $-4.5kcal\;mol^{-1}$. The sizes of the lipid vesicles were measured with dynamic light scattering instrument in order to get the correlation between the reaction enthalpies and the vesicle sizes. The bindings of SP on the lipid vesicles with diameter of 37 to 108 nm were classified into the enthalpy-driven reaction or the entropy-driven reaction according to the size of the lipid vesicles. For the enthalpy-driven binding reaction, the significance of the electrostatic interactions between SP and lipid molecules was affirmed from the experimental results of the DMPC/DMPG/DMPH and DMPC/DMPS/DMPH vesicles as well as the importance of the hydrophobic interactions between hydrophobic groups of SP and lipid molecules.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.130-130
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• 2012

Axial bindings of diatomic molecules to metalloporphyrins involve in the dynamic processes of biological functions such as respiration, neurotransmission, and photosynthesis. The binding reactions are also useful in sensor applications and in control of molecular spins in metalloporphyrins for spintronic applications. Here, we present the binding structures of diatomic molecules to surface- supported Co-porphyrins studied using scanning tunneling microscopy. Upon gasexposure, three-lobed structures of Co-porphyrins transformed to bright ring shapes on Au(111), whereas H2-porphyrins of dark rings remained intact. The bright rings are explained by the structures of reaction complexes where a diatomic ligand, tilted away from the axis normal to the porphyrin plane, is under precession. Our results are consistent with previous bulk experiments using X-ray diffraction and nuclear magnetic resonance spectroscopy.

• 한국언어정보학회지:언어와정보
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• 제5권2호
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• pp.9-20
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• 2001

Barker (1997) has argued that all the three approaches to anaphora, namely, E-type theories, Discourse Representation Theory, and Dynamic Semantics, are faced with the so-called 'double-bind' problem in the quantifier/variable-binding model of anaphora. Recently, in the same journal, Slater (2000) has claimed that the so-called epsilon account (Slater 1991, 1993, 1994, 1997) handles the problem without any difficulty. However, it will be proposed in this paper that the epsilon account has its own problems in handling the quantifier/variable-binding, and that as argued in Yoon (1998), the so-called‘conjunctive paraphrase’account is the correct solution to the problem, which is intuitively supported as well.

• KSBB Journal
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• 제13권2호
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• pp.125-132
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• 1998

Protein affinity membrane was prepared via the coating of chitosan gel on the porous flat polysulfone membrane surface, followed by the immobilization f the reactive dye (Cibacron Blue 3GA) to the chitonsan gel. The maximum protein binding capacity of affinity membrane was about 70${\mu}g/cm^2$ determined by the batch adsorption experiments of human serum albumin (HSA). Using module of this membrane, the characteristics of protein chromatography were investigated through the experiments of elution and frontal chromatography of HSA. This membrane module promises as a chromatography column, since it represented a lower pressure drop and a greater reproducibility. The protein separation ratio was significantly influenced by the flow rate of mobile phase and the injection quantity of HSA. The dynamic protein binding capacity of module decreased from the equilibrium binding capacity with increasing flow rate and approached the value of 15 - 20 ${\mu}g/cm^2$ for flow rates above 6 mL/min.

• Transactions on Electrical and Electronic Materials
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• 제3권1호
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• pp.23-29
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• 2002

The electronic properties of Carbon Nanotube(CNT) are currently the focus of considerable interest. In this paper, the electronic properties of finite length effect in CNT for the carbon nano-scale device is presented. To Calculate the electronic properties of CNT, Empirical potential method (the extended Brenner potential for C-Si-H) for carbon and Tight Binding molecular dynamic (TBMD) simulation are used. As a result of study, we have known that the value of the band gap decreases with increasing the length of the tube. The energy band gap of (6,6) armchair CNT have the ranges between 0.3 eV and 2.5 eV. Also, our results are in agreements with the result of the other computational techniques.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2494-2504
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• 2014

P38 mitogen activated protein (MAP) kinase is an important anti-inflammatory drug target, which can be activated by responding to various stimuli such as stress and immune response. Based on the conformation of the conserved DFG loop (in or out), binding inhibitors are termed as type-I and II. Type-I inhibitors are ATP competitive, whereas type-II inhibitors bind in DFG-out conformation of allosteric pocket. It remains unclear that how these allosteric inhibitors stabilize the DFG-out conformation and interact. Organosilicon compounds provide unusual opportunity to enhance potency and diversity of drug molecules due to their low toxicity. However, very few examples have been reported to utilize this property. In this regard, we performed docking of an inhibitor (BIRB) and its silicon analog (Si-BIRB) in an allosteric binding pocket of p38. Further, molecular dynamics (MD) simulations were performed to study the dynamic behavior of the simulated complexes. The difference in the biological activity and mechanism of action of the simulated inhibitors could be explained based on the molecular mechanics/generalized Born surface area (MM/GBSA) binding free energy per residue decomposition. MM/GBSA showed that biological activities were related with calculated binding free energy of inhibitors. Analyses of the per-residue decomposed energy indicated that van der Waals and non-polar interactions were predominant in the ligand-protein interactions. Further, crucial residues identified for hydrogen bond, salt bridge and hydrophobic interactions were Tyr35, Lys53, Glu71, Leu74, Leu75, Ile84, Met109, Leu167, Asp168 and Phe169. Our results indicate that stronger hydrophobic interaction of Si-BIRB with the binding site residues could be responsible for its greater binding affinity compared with BIRB.

• 디지털산업정보학회논문지
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• 제17권2호
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• pp.29-35
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• 2021

Recently, with the widespread use of the Internet of Things (IoT), the service structure has been studied to interact with various service domains. A common way to interact with other service domains is to develop the APIs needed to interact on the platform. However, to use a common method, we consider many costs and resources as APIs can increase while adding connections from other service domains. To address this issue, we propose the design and implementation of a dynamic API platform. The proposed platform can dynamically create APIs when requesting service applications, depending on the target service domain. To demonstrate the feasibility of the proposed platform, we develop a COVID-19 weekly infection status, regional infection status, and vaccination status service using dynamic APIs from the Public Data Portal using the proposed dynamic API platform and Node-RED.

• 전기의세계
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• 제31권4호
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• pp.281-287
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• 1982

PASCAL has a recursive nested structure and uses deep binding of identifiers. This paper studies the problems and techniques in storage management for PASCAL on the IBM 370 system, and presents run-time storage administration algorithms which use stack scheme and heap efficiently on the view of storage. The stack-scheme was used to implement the feature of recursive nested structure and the heap was used to implement the feature of the dynamic allocation procedure and pointer variable, allowing an additional dynamic storage recovery procedure.

• 정보처리학회논문지A
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• 제14A권2호
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• pp.117-124
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• 2007

최근 IT서비스 분야에서는 서비스들간의 자유로운 융.통합이 이슈로 부각되고 있으며 이를 위해 많은 소프트웨어 개발 벤더들은 SOA(Service-Oriented Architecture)기반의 통합 서비스 플랫폼을 제시하고 있다. 그러나 이러한 서비스 플랫폼들은 주로 엔터프라이즈 어플리케이션을 대상으로 하며 대규모의 복잡한 시스템에만 적재되어 운용되고 있다. 즉, 서비스 융.통합의 관점은 상위 수준의 서비스 어플리케이션에 초점을 맞추고 있으며 하위 수준의 임베디드 소프트웨어 분야는 고려되지 않고 있는 실정이다. 기존 서비스들과 최근 도입되고 있는 임베디드 소프트웨어 기술 기반의 서비스들과의 융.통합을 위해서는 장치에 대한 서비스화를 지원하는 기술이 필요하다. 본 논문에서는 임베디드 장치의 서비스화를 지원하는 SOA기반의 확장 가능한 구조를 가진 동적 서비스 연결 프레임워크를 제시한다. 또한 임베디드 보드 기반의 로봇 장치에 구현한 프레임워크를 적재하여 외부의 이기종 서비스나 장치들과의 다양한 상호작용을 데모 시나리오를 통하여 보여준다.

• 멤브레인
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• 제8권1호
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• pp.50-58
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• 1998

Polysulfone 재질의 다공성 평판막 및 실관막에 키토산 피막층을 형성시킨 후 반응성 염료인 Cibacron Blue 3GA를 고정화시켜 human serum albumin(HSA)의 결합용량이 최대 70 $\mu{g/cm}^2$인 단백질 친화성 막을 제조하였다. 친화성 평판막 모듈을 대상으로 HSA에 대한 용출 크로마토그래피 실험을 수행하여 eluent 용액의 최적 환경조건을 결정하였는바, 1M KCl이 첨가된 농도 0.06 M, pH 10의 universal buffer를 eluent로 사용했을 때 리간드와 결합된 단백질의 용출이 가장 우수하였다. 친화성 평판막 및 실관막 모듈을 대상으로 HSA의 전열 크로마토그래피 실험을 수행하여 단백질에 대한 동적 결합용량을 측정하였다. 이 결과 동적 결합용량은 평판막 모듈의 경우에는 loading 용액의 유량과 HSA의 농도가 증가함에 따라 평형 결합용량 값으로부터 크게 감소하였으나, 실관막 모듈의 경우에는 loading 용액의 유량과 HSA의 농도에 관계없이 항상 평형 결합용량 수준을 유지하였는바, 따라서 실관막 모듈이 평판막 모듈보다 단백질 친화성 크로마토그래피 분리관으로서 더 효과적이었다.