• 미생물학회지
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• 제32권3호
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• pp.198-201
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• 1994

Lactobacillus casei에 감염하는 bacteriophage J1 게놈의 cohesive end site (cos)의 염기배열을 결정하였다. 또한 환형 cos와 선형 J1 DNA의 왼쪽 말단 염기배열을 비교한 결과 terminase가 절단하는 위치는 다음과 같았다. 5'- GGTCGGCC$\downarrow$ -3' 3'- $\uparrow$CCAGCCGG -5' J1 게놈의 cohesive end는 3' 말단이 돌출되어 있으며 8개의 뉴클레오티드로 이루어져 있고 G+C 함유율이 87.5%이었다. cos 부위는 선형 DNA의 왼쪽 5' 말단 뉴클레오티드의 위치를 +1로 정하였을 때 -33부터 +25까지 대칭이었다. 지금까지 보고된 phage들의 cos 부위 사이에 상동성은 발견되지 않았다.

• 한국산학기술학회논문지
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• 제18권6호
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• pp.256-262
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• 2017

본 논문은 조직 내 지식 공유에 있어 동료 효과와 성별 효과를 검증하는 것을 목적으로 한다. 즉, 본 논문은 동료의 사회적 태만과 지식 공유 간 어떠한 관계가 있는지, 그리고 동료의 사회적 태만과 지식 공유 간 관계에 있어 성별 차이가 어떠한 상이한 영향을 미치는지를 살펴본다. 이를 위해 본 연구에서는 경제적 사회적 교환관계이론에 근거하여 동료의 사회적 태만에 대해 강하게 인지할수록 개인의 지식 공유 정도는 감소할 것이라고 예측하였다. 이에 더하여, 이러한 지식 공유 감소 효과는 여성에 비해 남성의 경우 보다 크게 작용할 것이라고 가설화하였다. 이러한 가설 검증을 위하여 상사-부하 설문(170부)을 바탕으로 위계적 회귀분석을 실시한 결과, 이론 부분에서 예측한 바와 같이 동료의 사회적 태만은 개인의 지식공유 행동을 저하시키는 것으로 나타났다. 뿐만 아니라, 이러한 지식 공유 저하 효과는 여성에 비해 남성의 경우 감소 폭이 보다 큰 것으로 나타났다. 이러한 이론적 논의와 실증 결과를 바탕으로 본 연구가 가진 함의와 한계를 결론에 제시한다.

• 아동학회지
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• 제8권1호
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• pp.1-14
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• 1987

The purpose of the present research was to study the self-regulation of the child during mother child interaction. That is. this study focused on how mothers regulated children's problem solving efforts before children began to function as independent agents capable of self-regulation. The emphasis was on the "regulation-by-others" that preceded "self-regulation". The subjects of this study were 30 mother-child dyads. The dyads were divided into 3 groups of 10 dyads each on the basis of the child's age (2, 3, and 4 years of age). The instrument used for this study was the "truck puzzle" devised by Wertsch, McNamee, Mclane and Budwig (1980). The problem solving task in this study required the dyad to make a puzzle in accordance with the model puzzle. The interaction of 30 mother-child dyads was observed and analyzed flH verbal and non-verbal behavior used by the mother to regulate the child's behavior. One-way ANOVA, Scheffe post hoc comparision and Spearman's rank-difference correlation were used for the statistical analysis of the data. The results revealed that there were significant age trends in regulation-by-others and self regulation in joint cognitive activity; that is, there were developmental transitions from regulation by-others to self-regulation in connection with crucial strategic behavior (looking at the model). Significant differences were found in mothers' interventions after children's gaze at the model in joint cognitive activity; that is, the mean proportion of mothers' intervention for the 2-year-old group was the highest and that of the 4-year-old group was the lowest. There were no significant age-related differences in mothers' use of referential perspectives in joint cognitive activity. Children's regulation- by-others increased and children's self-regulation decreased in proportion to mothers' use of referential perspectives.

• Applied Microscopy
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• 제15권1호
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• pp.71-85
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• 1985

The ultrastructure on the dorsal vessel of 5-day-old cabbage butterfly, Pieris rapae L., was carried out using the transmission and scanning electron microscope. The results are as follows. 1) The aorta. The aorta is simple tubular type and consists of the inner and outer membrane of the myocardium and thick myocardium is located between them. However the inner membrane with $0.26{\mu}m$ thickness and outer membrane with $0.08{\mu}m$ are composed of fibrous materials, the former is composed of low and high densed fibrous materials and the latter appears homogeneous layer. The myocardium consists of typical striated muscles. The sarcomere with $1.6{\mu}m$ length and in cross section, each thick filaments are surrounded by $7{\sim}8$ thin filaments. The intercalated disc is joining the end of the two muscle cells, desmosomes and septate junctions are appeared between the neighboring muscle cells. 2) The heart. The heart composing of myocardium enclosed by its inner and outer membrane as the aorta has a series of well formed segmental chamber. The arrangement of myofilaments, cell adhensions and membrane elements are observed as same as at the aorta. The inner membrane of the heart is deeply invaginated into the myocardium than the outer membrane and a lot of well developed mitochondria with rod shape are aggregated in the folds. The longitudinally and transversely oriented tubule system formed by invagnation of the sarcolemma into the muscle bundle is built up dyad with the sarcoplasmic reticulum as the aorta. The slit is formed by deeply invagination of the inner membrane of myocadium toward the muscle layer and then the inner and outer membrane of myocardium are fused. Therefore, the ostium is formed between the myocardium and situated at the lateral side of the myocardium.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.149-155
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• 1987

Growth and development of a higher plant, or any living organism for that matter, could be defined as an orderly expression of the genome in time and space in close interaction with the environment. During differentiation and development of a tissue or organ a group of genes must be selectively turned on or turned off mainly by trans-acting regulators. In this general concept of regulation of regulation of gene expression, a DNA molecule is recognized at a specific nucleotide sequence by DNA-binding factors. Molecular biology of the regulatory factors such as hormones, and their receptors, target DNA sequences and DNA-binding proteins are well advanced. What is not clearly understood is the molecular basis of the interactions between DNA and binding factors, expecially of the usages of the dyad symmetry of the target DNA sequences and the dimeric nature of the DNA-binding proteins. A unique 3-dimensional structure of DNA has been proposed that may play an important role in the orderly expression of the gene. A foldback intercoil (FBI) DNA configuration which was originally found by electron microscopy among mtDNA molecules from pearl millet has some unique features. The FBI configuration of DNA is believed to be formed when a flexible double helix folds back and interwines in the widened major grooves resulting in a four stranded, intercoil DNA whose thickness is the same as that of double stranded DNA. More recently, the FBI structure of DNA has been also induced in vitro by a novel enzyme which was purified from pearl millet mitochondria. It has been proposed that the FBI DNA could be utillized in intramolecular recombination which leads to inversion or deletion, and in intermolecular recombination which can lead to either site-specific recombination, genetic recombination via single strand invasion, or cross strand recombination. The structure and function of DNA in 3-dimensional aspect is emphasized for better understanding orderly expression of genes during growth and development.

• 한국보육지원학회지
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• 제10권6호
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• pp.43-56
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• 2014

본 연구에서는 자발적인 발화를 통해 유아의 구어 발화 특성을 분석하기 위해 놀이상황 유형별 구어 발화 자료를 수집하고, 구어 발화의 특성을 어절, 단어, 형태소 단위 평균발화길이와 어휘다양도로 구분하여 분석하고자 하였다. 이를 위해 본 연구에서는 5세 유아 32명을 대상으로 또래와의 친숙한 놀이상황 및 낯선 놀이상황에서의 대화를 수집하였다. 수집된 언어자료는 CSB(2014) 프로그램에 의해 전사 및 분석되었다. 본 연구의 결과는 다음과 같다. 첫째, 친숙한 놀이상황에서 여아의 어절, 단어, 형태소 단위 평균발화길이는 남아보다 더 긴 것으로 나타났다. 둘째, 유아의 단어 단위 평균발화길이는 친숙한 놀이상황보다 낯선 놀이상황에서 더 긴 것으로 나타났으며, 어휘다양도도 친숙한 놀이상황보다 낯선 놀이상황에서 더 높은 것으로 나타났다. 이와 같은 연구결과에 기초하여 유아의 자발적 발화 수집, 전사 및 분석프로그램의 중요성에 대해 논의한 후 향후 과제와 제언을 제시하였다.

• Molecules and Cells
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• 제21권1호
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• pp.129-134
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• 2006

Lon, also known as protease La, belongs to a class of ATP-dependent serine protease. It plays an essential role in degradation of abnormal proteins and of certain short-lived regulatory proteins, and is thought to possess a Ser-Lys catalytic dyad. To examine the structural organization of Lon, we performed an electron microscope analysis. The averaged images of Lon with end-on orientation revealed a six-membered, ring-shaped structure with a central cavity. The side-on view showed a two-layered structure with an equal distribution of mass across the equatorial plane of the complex. Since a Lon subunit possesses two large regions containing nucleotide binding and proteolytic domains, each layer of the Lon hexamer appears to consist of the side projections of one of the major domains arranged in a ring. Lon showed a strong tendency to form hexamers in the presence of $Mg^{2+}$, but dissociated into monomers and/or dimers in its absence. Moreover, $Mg^{2+}$-dependent hexamer formation was independent of ATP. These results indicate that Lon has a hexameric ring-shaped structure with a central cavity, and that the establishment of this configuration requires $Mg^{2+}$, but not ATP.

• 한국산학기술학회논문지
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• 제22권2호
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• pp.625-631
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• 2021

본 연구는 개인 수준에서 조직시민행동이 초래하는 딜레마적 상황을 보다 잘 이해하기 위해서, 조직시민행동에 영향을 미치는 개인적 특성인 자기 비판적 완벽주의 성격과 성별효과를 검증하고자 한다. 즉, 자원보존이론을 바탕으로자기 비판적 완벽주의 성격이 조직시민행동에 미치는 영향에 대해 논의하고, 성별 차이가 이 관계를 어떻게 변화 시키는지를 살펴보고자 한다. 본 연구에서는 자원보존이론에서 주장하듯이, 자기 비판적 완벽주의 성격은 조직시민행동에 부정적인 영향을 줄 것으로 예측하였다. 또한 성별 관련 논의들에 근거하여 여성에 비해 남성일 경우 자기 비판적 완벽주의와 조직시민행동 간 부(-)의 관계가 강하게 나타날 것임을 가설로 제시하였다. 이러한 가설들을 검증하기 위하여 한국 기업들을 대상으로 상사-부하 설문을 실시하였고, 총 150쌍의 데이터를 사용하여 위계적 선형모형 분석을 실시하였다. 분석결과 자기 비판적 완벽주의 성격과 조직시민행동 간에 부(-)적인 영향이 있음을 확인 하였고, 이러한 부적 효과는 남성의 경우 더 강하게 나타남을 확인 하였다. 본 연구는 조직시민행동을 설명하는 개인적 특성과 성별효과를 살펴봄으로 인해, 조직시민행동 관련된 분야에 이론적·실무적 함의를 제공한다고 할 수 있다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제25권3호
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• pp.194-210
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• 2022

Human milk contains a number of nutritional and bioactive molecules including microorganisms that constitute the so-called "Human Milk Microbiota (HMM)". Recent studies have shown that not only bacterial but also viral, fungal, and archaeal components are present in the HMM. Previous research has established, a "core" microbiome, consisting of Firmicutes (i.e., Streptococcus, Staphylococcus), Proteobacteria (i.e., Serratia, Pseudomonas, Ralstonia, Sphingomonas, Bradyrhizobium), and Actinobacteria (i.e., Propionibacterium, Corynebacterium). This review aims to summarize the main characteristics of HMM and the role it plays in shaping a child's health. We reviewed the most recent literature on the topic (2019-2021), using the PubMed database. The main sources of HMM origin were identified as the retrograde flow and the entero-mammary pathway. Several factors can influence its composition, such as maternal body mass index and diet, use of antibiotics, time and type of delivery, and mode of breastfeeding. The COVID-19 pandemic, by altering the mother-infant dyad and modifying many of our previous habits, has emerged as a new risk factor for the modification of HMM. HMM is an important contributor to gastrointestinal colonization in children and therefore, it is fundamental to avoid any form of perturbation in the HMM that can alter the microbial equilibrium, especially in the first 100 days of life. Microbial dysbiosis can be a trigger point for the development of necrotizing enterocolitis, especially in preterm infants, and for onset of chronic diseases, such as asthma and obesity, later in life.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제26권2호
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• pp.88-98
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• 2023

Purpose: Children with inflammatory bowel disease (IBD) frequently undergo clinical assessments, involving triadic communication between clinician, parent, and child. During such encounters parents are traditionally the main communicator of information on their child's IBD, including subjective symptom reports. The level of agreement between children and their parents for IBD symptoms is poorly understood, and this study aimed to examine this factor. Methods: This was a cross-sectional study among children with IBD, and one parent. A validated paediatric IBD symptom report tool (IBDnow) enabled children and their parent to rate seven pain, well-being, and stool metrics, with dyads completing the tool concurrently. Results were assessed using: Individual agreement: proportion of identical symptom reports by each dyad (ideal score >0.7); Category agreement: percentage of identical reports for IBDnow metrics for the cohort; Inter-rater reliability: Gwet's AC1 coefficient with higher scores indicating better reliability (maximum=1). Results: Seventy-four parent/child dyads participated; child's mean age 12.2 years (standard deviation [SD] 2.9, range 6-16), mean time since diagnosis 2.8 years (SD 3), 54% female, 73% had Crohn's Disease. Mean individual agreement level was 0.6, with 27% of dyads agreeing on ≥6/7 IBDnow metrics. Category agreement was reported by 61% of dyads, 20% of parents overestimated, and 19% underestimated, their child's symptoms. Inter-rater reliability ranged from fair to good. Conclusion: These results should improve clinician awareness of how IBD symptom reports from parents may introduce bias. Children should be considered the most important source of symptom reports, and tools such as IBDnow utilised to enhance communication.