• Food Science of Animal Resources
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• v.41 no.2
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• pp.324-334
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• 2021

This study aimed at determining the genetic and virulence characteristics of the Listeria monocytogenes from smoked ducks. L. monocytogenes was isolated by plating, and the isolated colonies were identified by PCR. All the obtained seven L. monocytogenes isolates possessed the virulence genes (inlA, inlB, plcB, and hlyA) and a 385 bp actA amplicon. The L. monocytogenes isolates (SMFM2018 SD 1-1, SMFM 2018 SD 4-1, SMFM 2018 SD 4-2, SMFM 2018 SD 5-2, SMFM 2018 SD 5-3, SMFM 2018 SD 6-2, and SMFM 2018 SD 7-1) were inoculated in tryptic soy broth (TSB) containing 0.6% yeast extract at 60℃, followed by cell counting on tryptic soy agar (TSA) containing 0.6% yeast extract at 0, 2, 5, 8, and 10 min. We identified five heat resistant isolates compared to the standard strain (L. monocytogenes ATCC13932), among which three exhibited the serotype 1/2b and D-values of 5.41, 6.48, and 6.71, respectively at 60℃. The optical densities of the cultures were regulated to a 0.5 McFarland standard to assess resistance against nine antibiotics after an incubation at 30℃ for 24 h. All isolates were penicillin G resistant, possessing the virulence genes (inlA, inlB, plcB, and hlyA) and the 385-bp actA amplicon, moreover, three isolates showed clindamycin resistance. In conclusion, this study allowed us to characterize L. monocytogenes isolates from smoked ducks, exhibiting clindamycin and penicillin G resistance, along with the 385-bp actA amplicon, representing higher invasion efficiency than the 268-bp actA, and the higher heat resistance serotype 1/2b.

• Korean Journal of Environmental Biology
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• v.33 no.4
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• pp.476-483
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• 2015

We are aim to evaluate lysozyme and antibacterial activity of cultured catfish, Silurus asotus, that fed supplementally with Galla rhois extracts for eight weeks. Lysozyme activity in the spleen and serum of administrated group was higher than not administrated group, but in mucus of the lysozyme activity was no regular than other organ. The lysozyme activity of the spleen, kidney, serum of administrated fishes were increased after 2 weeks and that was highest after 8 weeks. Ht and GLU in serum of administrated fishes were gradually increased but GOT was decreased after 8 weeks. There is no significant differences in HB (Hemoglobin) and TP (Total Protein) each groups. Furthermore, there is no pathohistological changes of kidney and liver of tested fishes. The cumulative survival rates of administrated group after intraperitoneal injection of Aeromonas veronii with $6.5{\times}10^6cfu\;mL^{-1}$ was presented 33% in 9 days. As the Results, Galla rhois extracts has any beneficial effects for immunity elevation and antibacterial activity in catfish, Silurus asotus.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.513-516
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• 2005

SVC(Scalable Video coding) is the standardization of MPEG that aims to support multi spatial, temporal, and quality layers. We can support the video service guaranteeing the QoS in varied network circumstance by using SVC bitstream. In this paper, we propose the real-time SVC bitstream extractor that is able to extract the bitstream with varied frame rate and SNR quality in real-time. To do, extraction processing needs to be performed by GOP unit. As well , essential bitstream information for real-time extraction is aquired before doing extraction process. The proposed method is implemented by using JSVM 2.0. Experimental results show that the proposed method is valid.

• Korean Journal of Computational Design and Engineering
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• v.18 no.4
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• pp.250-257
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• 2013

This paper introduces a failure analysis procedure that underpins real-time fault prognosis. In the previous study, we developed a systematic eventization procedure which makes it possible to reduce the original data size into a manageable one in the form of event logs and eventually to extract failure patterns efficiently from the reduced data. Failure patterns are then extracted in the form of event sequences by sequence-mining algorithms, (e.g. FP-Tree algorithm). Extracted patterns are stored in a failure pattern library, and eventually, we use the stored failure pattern information to predict potential failures. The two practical case studies (marine diesel engine and SIRIUS-II car engine) provide empirical support for the performance of the proposed failure analysis procedure. This procedure can be easily extended for wide application fields of failure analysis such as vehicle and machine diagnostics. Furthermore, it can be applied to human health monitoring & prognosis, so that human body signals could be efficiently analyzed.

• Korean Journal of Pharmacognosy
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• v.39 no.4
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• pp.300-304
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• 2008

The root of Astragalus membranaceus Bunge (Leguminosae) has been used in the Korean oriental medicine for strengthening the vital energy. UV irradiation has been suggested as a major cause of photo aging in skin. In order to investigate protective effects against UV induced cellular damage, Astragali Radix was extracted with 70% ethanol and dissolved in DMSO. The protective effect was detected by MTT assay, LDH assay, and Comet assay in immortalized human keratinocyte cell line, HaCaT cell system after UV irradiation. Astragli Radix 70% EtOH extract reduced UV induced cellular damage in cell survival, membrane integrity and DNA damage.

• Journal of Pharmaceutical Investigation
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• v.18 no.3
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• pp.125-131
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• 1988

This study was undertaken to investigate the proteolytic enzyme from Neungee mushroom [Sarcodon aspratus (Berk) S. Ito]. The proteolytic activity of Neungee was higher than other several edible mushrooms under various pHs. The potency of proteolytic enzyme of Neungee was same as the digestive drugs containing protease. So the proteolytic activity of the enzyme was increased in neutral or weak alkaline pH, whose characteristics would be alkaline protease. The specific activity of the purified enzyme obtained by using Tris acryl CM-cellulose ion exchange increased 20 times as compared with that of the crude extract. The proteolytic enzyme was stable at room temperature, but decomposition was fast when incubated at higher temperature more than $40^{\circ}C$. The half life of the enzyme was longest in neutral pH and rate constant was increased in acidic or alkaline solution.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.135-139
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• 2005

Antioxidant activity of monascus pigment of Monascus purpureus P-57 mutant was studied. Methanol extract from monascus pigment was separated into five organic solvent fractions; hexane, chloroform, ethyl acetate, butanol and water fractions. Hexane fraction showed the highest free radical scavenging effect on 1,l-diphenyl-2-picryl hydrazyl(DPPH), and the strongest inhibitory effect against xanthine oxidase, followed by chloroform fraction. But butanol and water fraction did not show inhibitory effect against the enzyme. Lineweaver-Burk plot showed that hexane fraction inhibited xanthine oxidase by non-competitive mode.

• The Plant Pathology Journal
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• v.15 no.3
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• pp.168-171
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• 1999

Crude extracts of pathogenic Streptomyces turgidiscabies isolates produced necrotic reaction on potato slices. Necrosis was first visible 24 hr after application and increased in severity over several days. However, necrosis was not observed when crude extracts of nonpathogenic strains were applied onto tuber slices. Presence of thaxtomin A from crude extracts of pathogenic S. turgidiscabies was identified by thin layer chromatography and high performance liquid chromatography. Nonpathgenic strains did not produce thaxtomin A in oatmeal broth. Inoculation of potato tuber slices with thaxtomin A partially purified from crude extract of a pathogenic S. turgidiscabies ST5 reproduce necrotic reaction suggesting that thaxtomin A is a pathogenicity determinant in S. turgidiscabies.

• Proceedings of the KSRS Conference
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• 2008.03a
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• pp.20-24
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• 2008

차량에 부착된 CCD 카메라를 이용하여 취득된 도로 주변의 영상에 존재하는 사람의 얼굴을 추출하여 제거하는 처리를 할 경우, 사생활 침해의 문제 없이 사용자들에게 원하는 지역의 도로영상의 제공이 가능해진다. 이 실험의 목적은 차량에서 취득된 도로 주변의 칼라 영상에서 사람의 얼굴을 자동으로 추출하는 기술을 개발하는데에 있다. 도로 주변의 CCD영상에서의 얼굴 추출을 위해, HSI(색상, 채도, 명도) 칼라 모델과 YCrCb 칼라 모델을 사용하여 이들 모델에 임계값을 적용하여 피부색을 검출하였으며, 두 개의 모델을 사용한 결과 효과적인 피부색의 검출이 가능함을 확인할 수 있었다. 검출된 피부색 영역을 연결성과 밝기 차이를 이용하여 클러스터링을 실행하고 이렇게 나뉘어진 각각의 구역들에 구역의 면적, 구역내 존재하는 화소의 개수, 구역의 가로와 세로 비율 그리고 타원조건을 적용하여 얼굴 후보 구역을 결정하였다. 그리고 최종적으로 남겨진 구역을 이진화 하고, 이진화 된 영상 중 검은 부분이 5% 이상일 때 이들을 눈, 코, 입 등으로 간주하여 최종적인 얼굴로 결정하였다. 실험 결과 추출되지 않은 얼굴과 잘못 추출된 구역이 발생했으나, 얼굴에 해당하는 임계값등의 조건을 약화시킬 경우 대부분의 얼굴의 추출이 가능할 것으로 여겨지며, 추출된 구역을 흐리게 처리할 경우 오인식된 부분에 대한 사용자의 거부감도 줄일 수 있을 것 으로 예상된다.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.115-126
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• 2004

The actinomycete strain PK-A41 was isolated from a soil sample from pepper fields in Ko-yang, Korea. The strain PK-A41 inhibited the mycelial growth of some plant pathogenic fungi and oomycete, Alternaria mali, Colletotrichum orbiculare, Fusarium oxysporum f.sp. lycopersici, Magnaporthe grisea, Rhizoctonia solani, and Phytophthora capsici. The presence of LL-diaminopi-melic acid in the cell wall extract and the nucleotide sequence of the 16S rDNA region of the strain PK-A41 was assigned to Streptomyces scabiei. Further morpho-logical, biochemical, and pathological analyses also confirmed the strain PK-A41 to be S. scabiei, which is pathogenic to potato tubers. The maximum antibiotic production of the strain PK-A41 was achieved when grown on the glycerol peptone broth (GPB) medium for 9 days.