Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Dietary Supplementation with Galla rhois Extract on Lysozyme and Antibacterial Activity of the Cutured Catfish (Silurus asotus)

양식 메기의 라이소자임 활성 및 항 균력에 미치는 오배자 추출물의 투여 효과

  • Kim, Jin-Do (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Lee, Nam-Sil (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Choi, Hye-Sung (Inland Aquacutural Research Center, NFRDI) ;
  • Kim, Kyoung-Duck (Aquafeed Research Center, NFRDI) ;
  • Jo, Hyae-in (Gyeongbuk Native Fish Business Center)
  • 김진도 (국립수산과학원 병리연구과) ;
  • 이남실 (국립수산과학원 병리연구과) ;
  • 최혜승 (국립수산과학원 내수면양식연구센터) ;
  • 김경덕 (국립수산과학원 사료연구센터) ;
  • 조혜인 (경상북도 토속어류산업화센터)
  • Received : 2015.11.02
  • Accepted : 2015.12.15
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

We are aim to evaluate lysozyme and antibacterial activity of cultured catfish, Silurus asotus, that fed supplementally with Galla rhois extracts for eight weeks. Lysozyme activity in the spleen and serum of administrated group was higher than not administrated group, but in mucus of the lysozyme activity was no regular than other organ. The lysozyme activity of the spleen, kidney, serum of administrated fishes were increased after 2 weeks and that was highest after 8 weeks. Ht and GLU in serum of administrated fishes were gradually increased but GOT was decreased after 8 weeks. There is no significant differences in HB (Hemoglobin) and TP (Total Protein) each groups. Furthermore, there is no pathohistological changes of kidney and liver of tested fishes. The cumulative survival rates of administrated group after intraperitoneal injection of Aeromonas veronii with $6.5{\times}10^6cfu\;mL^{-1}$ was presented 33% in 9 days. As the Results, Galla rhois extracts has any beneficial effects for immunity elevation and antibacterial activity in catfish, Silurus asotus.

Keywords

References

  1. Cha C-N, E-H Yu, E-K Park, S Kim and H-J Lee. 2013. Effects of Dietary Supplementation with Galla Rhois on Growth Performance and Diarrhea Incidence in Postweaning Piglets. J. Vet. Clin. 30:353-358.
  2. Cho HS, S-W Kang, J-H Kim, M-J Choi, H-W Yu, E Park and H-S Chun. 2014. Antioxident and Antimicrobial Activities of Combined Extracts of Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis. Kor. Soc. Biotech. Bioeng. Journal 29:29-35.
  3. Cho JY, I Choi and E-K Hwang. 2003. Antimicrobial activity of extracts from medicinal herbs against Escherichia coli. Korea J. Vet. Res. 43:625-631.
  4. Cho YH, D-H Choi and S-H Choi. 2005. Effect of Mistletoe (Viscum album Coloratum) on the Non-Specific Immune Responses in Japanese Eel (Anguilla japonica). J. Fish Pathol. 18:59-66.
  5. Choi HS. 2003. Antibacterial activities of extracts of Galla rhois on fish pathogenic bacteria and physiological response in fishes. Ph. D. dissertation.
  6. Choi MY, E-J Choi and E Lee. 1999. Effect of Rhus chinensis gall extract on liver function, plasma lipid composition and antioxidant system in rats with high fat diet. J. Korean Soc. Food Sci. Nutr. 28:632-637.
  7. Chun NC, E-A Yu, E-K Park, S Kim and H-J Lee. 2013. Effects of dietary supplementation with Galla Rhois on growth performance and diarrhea incidence in postweaning piglets. J. Vet. Clin. 30:353-358.
  8. Jee BY, J-S Seo, E-J Jeon, E-H Lee, H-J Choi, J-D Kim, S-H Jung and M-A Park. 2012. Effects of various concentrations of skullcap extract in the diets on disease resistance of olive flounder Paralichthys olivaceus. J. Fish Pathol. 25:21-30. https://doi.org/10.7847/jfp.2012.25.1.021
  9. Jo HS, S-W Kang, J-H Kim, M-J Choi, H-W Yu, E Park and H-S Chun. 2014. Antitoxidant and antimicrobial activities of combined extracts of Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis. Korean Soc. Biotechnol. Bioeng. J. 29:29-35.
  10. Jo MR, J-W Kim and D-S Kim. 2002. Antimicrobial Effects of Natural Plant and Mushroom, Dicyophora indusiata Extracts on Fish Pathogenic Bacteria. J. Korean Fish. Soc. 35:578-582.
  11. Jung SH, J-S Seo, J-D Kim, H-S Choi and M-A Park. 2011. Application of automatic dry chemistry analyzer (FUJI DRY-CHEM 3000) used to hematological analysis of cultured freshwater fish in low temperature season. J. Fish Pathol. 24:247-254. https://doi.org/10.7847/jfp.2011.24.3.247
  12. Jung YH, D-H Kim, H-Y Kim, T-S Shin, M-J Oh, J-H Lee, J-H Kim, S-Y Im and E-H Kim. 2010. Effects of diets supplemented with Yuzu Citrusjunos Sieboldex Tanaka on disease resistance of olive flounder, Paralichthys olivaceus. J. Fish Pathol. 23:389-398.
  13. Kim BM, T-H Kwon and K-Y Chai. 2008. Studies on the Cytoprotective and Anti-inflammatory Effects of Isolate from Galla Rhois. Korean J. Orient. Physiol. Pathol. 22:1449-1453.
  14. Kim JD, Y-C Kim, K-S Kim, S-H Woo and S-W Park. 2009. The effect of omae Prunus mume extract on the immune response and growth rate of Japanese eel Anguilla japonica. J. Fish Pathol. 22:367-374.
  15. Kim JW, S-I Park and S-K Chun. 1992. Purification and antibacterial effect of lysozyme from flounder, Paralichthys olivaceus. J. Fish Pathol. 5:87-92.
  16. Kim KH, A-R Kim, E-J Cho, S-J Joo, J-H Park, J-Y Moon, J-H Yum, T-H Kim, H-J Kwon, H-T Lee, Y-M Kim and E-W Lee. 2014. Antibacterial activity of Rhus javanica against the fish pathogens Vibrio ichthyoenteri and Streptococcus iniae. Kor. J. Fish Aquat. Sci. 47:18-22.
  17. Kim YG, B-J Rho and K-K Lee. 1994. Antimicrobial activity of Artemisia princeps var. orientalis essential oil against fish pathogenic bacteria. J. Fish Pathol. 7:105-112.
  18. Kwon MK, Y-C Kim, Y-C Sohn and S-i Park. 1999. The dietary supplementing effects of Kugija, Lycium chinense, on immune response of Nile tilapia, Oreochromis niloticus, to Edwardsiella tarda. J. Fish Pathol. 12:73-81.
  19. Lee HA, S-H Hong, Y-H Chung, K-D Song and O Kim. 2012. Anticoccidal effects of Galla rhois extract on Eimeria tenella-infe3cted chicken. Lab. Anim. Res. 28:193-197. https://doi.org/10.5625/lar.2012.28.3.193
  20. Lee JJ, J-H Bae, D-H Kim, J-J Lim, D-G Kim, H-J Lee, W-G Min, M-H Rhee, H-H Chang, H Park and S Kim. 2011. Intracellular replication inhibitory effects of Galla rhois ethanol extract for Burucella abortus infection. J. Ethanopharmacol. 138:602-609. https://doi.org/10.1016/j.jep.2011.10.007
  21. Lee NS, S-H Jeong and B-Y Jee. 2010. Anti-fish pathogenic efficacy of hot water extracts obtained from 5 herbs in-vitro and efficacy and toxicity in flounder of the one selected herb, skullcap. J. Fish Pathol. 23:137-143.
  22. Park SM, S-I Park, M-D Huh and Y-K Hong. 1999. Inhibitory effect of green tea on collagenase activity and growth of fish pathogenic bacteria. J. Fish Pathol. 12:83-88.
  23. Parry RM, R-C Chandau and R-M Shahani. 1965. A rapid and sensitive assay of muramidase. Pro. Soc. Exp. Biol. Med. 119:384-386. https://doi.org/10.3181/00379727-119-30188
  24. Takahashi Y, T Itami and K Konekawa. 1986. Enzymatic properties of partially lysozyme from the skin mucus of carp. Bull. Jap. Soc. Sci. Fish. 52:1209-1214. https://doi.org/10.2331/suisan.52.1209