• 대한한의정보학회지
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• 제15권1호
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• pp.57-66
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• 2009

Objectives : The aim of the present study is to evaluate the wound healing-enhancing and anti-inflammatory effects of Pinus densiflora, Cornus officinalis, Zingiber officinale, Ganoderma japonicum and Scutellaria baikalensison human keratinocyte, HaCaT cells. Methods : We adopted in vitro wound healing assay to measure the proliferation-and migration-enhancing effects in HaCaT cells. The expressions of cytokine genes were measured in HaCaT cells using real-time PCR analysis. Results : The extracts of Pinus densiflora, Cornus officinalis, Zingiber officinale, Ganoderma japonicum and Scutellaria baikalensis enhanced the proliferation and migration of HaCaTcells. The expression of keratinocyte growth factor receptor(FGFR2-IIIb) gene was also induced. The extracts inhibited iNOS, IL-$1{\beta}$ and TNF-$\alpha$ gene expression. Conclusions : The extract of Pinus densiflora, Cornus officinalis, Zingiber officinale, Ganoderma japonicum and Scutellaria baikalensis has wound healing-enhancing effects and anti-inflammatory effects.

• 한국약용작물학회지
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• 제13권5호
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• pp.298-302
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• 2005

In order to decide the optimum planting time for Bupleurum falcatum L. which is grown widely in Korea, some experiments were carried out in different regions such as mid-north area (Gangwon-do, Gyeonggi-do and Chungcheongnamdo), mid-south area (Jeollanam-do, Jeollabuk-do, Gyeongsangnam-do and Gyeongsangbuk-do). The mid-south area had the highest total saikosaponin contents of 1.001, which is the medicinal ingredients of Bupleurum falcatum L., in accordance with the different Bupleurum falcatum L. regions. Also this area has the highest extract contents of 24.3%. Average dry root yields in mid-north area (Gangwon-do) were $450{\sim}460$ kg/ha at planting time of March 20th to March 30th, 500 kg/ha at the time of March 30th to April 10th, and $470{\sim}480$ kg/ha at the time of March 10th to March 20th in Chungcheongnam-do. The optimum planting time in the mid-south area (Jeollabuk-do) was March 10th to March 30th with the average dry root yield of $490{\sim}550$ kg/ha. Average dry root yield in Euiseong, Gyeongsangbuk-do area was 470 kg/ha, and the optimum planting time was April 10th, which suggested that the yield increased as the planting time was delayed. At Jeju-do, the volcanic soil of farsouthern area, average dry root yield was 510 kg/ha at the time of March 10th, but the yield from non-volcanic soil was lower than from volcanic soil, which had a dry root yield of 470 kg/ha.

• 한국산업정보학회논문지
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• 제17권4호
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• pp.71-85
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• 2012

본 논문은 운항중인 선박에서 기록되어지는 운항정보 및 엔진 가동정보 등을 실시간으로 모니터링하고, 문제 발생 시에 그 근본원인을 찾아내어 민첩하게 대응할 수 있는 일련의 결함원인 분석 및 예방시스템 개발을 목적으로 한다. 결함분석을 위해서는 선박엔진의 주요기관에 부착된 센서들로부터 장기간 수집된 정보를 사용하게 되는데, 이 양이 매우 방대하며, 잡음 및 중복정보(Redundancy)가 너무 많이 포함되어, 수집된 센서 데이터를 바로 고장분석에 사용하기에는 어려움이 있다. 따라서 본 논문에서는 방대한 양의 데이터 중, 정보의 손실을 최소화하고 중요한 정보만을 추출하기 위해 'Equal-frequency binning'과 'Entropy' 기반의 데이터 필터링 방법에 관해 연구하였다. 실제로 시험운용 중인 선박엔진 데이터를 개발된 선박엔진 고장분석 소프트웨어를 이용하여 결함분석을 수행하여, 제안된 방법의 효용성을 검증한다.

• Toxicological Research
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• 제29권3호
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• pp.195-201
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• 2013

The anti-inflammatory effects of glycosaminoglycan (GAG) derived from Isaria sinclairii (IS) and of IS extracts were investigated in a complete Freund's adjuvant (CFA)-treated chronic arthritis rat model. Groups of rats were treated orally with 30 mg/kg one of the following: [1] saline control, extracts of [2] water-IS, [3] methanol-IS, [4] butanol-IS, [5] ethyl acetate-IS, or [6] Indomethacin(R) as the positive control for a period of two weeks. The anti-paw edema effects of the individual extracts were in the following order: water-IS ex. > methanol ex. > butanol ex. > ethyl acetate ex. The water/methanol extract from I. sinclairii remarkably inhibited UV-mediated upregulation of NF-${\kappa}B$ activity in transfected HaCaT cells. GAG as a water-soluble alcohol precipitated fraction also produced a noticeable anti-edema effect. This GAG also inhibited the pro-inflammatory cytokine levels of prostaglandin $E_2$-stimulated lipopolysaccharide in LAW 264.7 cells, cytokine TNF-${\alpha}$ production in splenocytes, and atherogenesis cytokine levels of vascular endothelial growth factor (VEGF) production in HUVEC cells in a dose-dependent manner. In the histological analysis, the LV dorsal root ganglion, including the articular cartilage, and linked to the paw-treated IS GAG, was repaired against CFA-induced cartilage destruction. Combined treatment with Indomethacin(R) (5 mg/kg) and IS GAG (10 mg/kg) also more effectively inhibited CFA-induced paw edema at 3 hr, 24 hr, and 48 hr to levels comparable to the anti-inflammatory drug, indomethacin. Thus, the IS GAG described here holds great promise as an anti-inflammatory drug in the future.

• 한국약용작물학회지
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• 제10권3호
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• pp.200-205
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• 2002

길경의 부탄올 분획으로부터 지표성분을 분리, 정제하기 위해 $SiO_2$ 컬럼크마토그래피를 실시한 결과 1종(화합물 1)의 단일 물질을 순수 분리하였으며 화합물 1의 구조는 $^1H$, $^{13}C$ NMR 등 분광학적 관측 결과 platycodin D로 확인되었다. Platycodin D의 HPLC 정량법을 검토한 결과, 검출기는 증기화광산란검출기(ELSD)를, 컬럼은 역상컬럼을 사용하여 길경 MeOH 추출액으로부터 효율적인 지표성분 정량이 가능하였다. 추출조건을 재검토한 결과 환류추출보다는 초음파 추출이 더 효율적이었으며 추출용매로는 80% MeOH이 다른 용매조건보다 추출효율이 우수하였다. 건조조건의 검토에서는 platycodin D의 함량이 양건이나 양건과 건조기 혼용건조 보다 건조기 단독 건조시 더 높았으며 특히 $60^{\circ}C$ 열풍건조에서 그함량이 0.083%로서 가장 높았다. 묽은 에탄올 엑스 함량도 건조기만을 이용한 열풍건조가 양건과 건조기 열풍건조를 혼용한 방법보다 4% 정도 더 높았다.

• 자원환경지질
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• 제30권5호
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• pp.459-467
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• 1997

In order to examine the primary trends and characteristics of geological lineaments along the southwestern boundary of Okcheon zone, we carried out the analysis of geological lineament trends over six selected sub-areas using Landsat-5 TM images and digital elevation model. The trends of lineaments is determined by a minimum variance method, and the resulting geological lineament map can be obtained through generalized Hough transform. We have corrected look direction biases reduces the interpretability of remotely sensed image. An approach of histogram modification is also adopted to extract drainage pattern specifically in alluvial plains. The lineament extracting method adopted in this study is very effective to analyze geological lineaments, and that helps estimate geological trends associated various with the tectonic events. In six sub-areas, the general trends of lineaments are characterized NW, NNW, NS-NNE, and NE directions. NW trends in Cretaceous volcanic rocks and Jurassic granite areas may represent tension joints that developed by rejuvenated end of the Early Cretaceous left-lateral strike-slip motion along the Honam Shear Zone, while NE and NS-NNE trends correspond to fault directions which are parallel to the above Shear Zone. NE and NW trends in Granitic Gneiss are parallel to the direction of schitosity, and NS-NNE and NE trends are interpreted the lineation by compressive force which acted by right-lateral strike-slip fault from late Triassic to Jurassic. And in foliated Granite, NE and NNE trends are coincided with directions of ductile foliation and Honam Shear Zone, and NW-NNW trends may be interpreted direction of another compressional foliation (Triassic to Early Jurassic) or end of the Early Cretaceous tensional joints. We interpreted NS-NNE direction lineation is related with the rejuvenated Chugaryung Fault System.

• 지능정보연구
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• 제9권2호
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• pp.101-119
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• 2003

Achieving the beneficiary goal of recent discovery in human genome project still needs a way to retrieve and analyze the exponentially expanding bio-related information. Research on bio-related fields naturally applies knowledge discovered to the current problem and make inferences to extract new information where shared concepts and data containing information need to be defined and used in a coherent way. In such a professional domain, while the need to help users reduce their work and to improve search results has been emerged, methods for systematic retrieval and adequate exchange of relevant information are still in their infancy. The design of our system aims at improving the quality of information retrieval in a professional domain by utilizing both corpus-based and concept-based ontology. Meta-rules of helping users to make an adequate query are formed into an ontology in the domain. The integration of those knowledge permits the system to retrieve relevant information in a more semantic and systematic fashion. This work mainly describes the query models with details of GUI and a secondary query generation of the system.

• 대한토목학회논문집
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• 제31권2B호
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• pp.121-128
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• 2011

본 연구에서는 과거 60년 간 한반도에 영향을 미친 태풍의 강도(중심기압)와 지속기간에 대한 경향 분석을 실시하였다. 경향 성분 추출을 위해 Singular Spectrum Analysis(SSA)를 사용하였고 경향 성분에 대한 선형회귀분석 결과 태풍의 강도가 약간 증가하는 것을 볼 수 있었다. 또한 약 30년의 장주기 변화를 발견하였고, 이에 따라 전체 시계열을 30년 기간의 두 개의 하위 기간으로 나눠서 태풍의 중심기압 및 지속기간에 대한 정규분포 및 Gumbel 분포를 추정하였다. 그 결과 두 번째 기간에 평균적인 태풍의 중심기압은 감소하지만 수퍼 태풍에 해당하는 매우 큰 태풍의 비율은 거의 변화가 없음을 발견하였다. 지속기간에 대해서도 두 번째 기간에 뚜렷한 증가가 있음을 발견하였다.

• 한국물환경학회지
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• 제32권6호
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• pp.628-648
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• 2016

Increased attention has been paid to the presence of veterinary antibiotics in various environmental matrices due to their toxicological behavior in the ecosystem and development of antibiotic-resistant strains of pathogenic bacteria. In the this review, 37 target antimicrobials were selected based on annual sales of antibiotics for livestock in South Korea 2014. Also, extraction and clean-up methods for the determination of the antibiotic residues in liquid samples including water, milk, and honey were comprehensively reviewed in the literature. Solid-phase extraction (SPE) was commonly used as a pre-treatment method for the samples. Most of the analytes were extracted in acidic conditions (2.5~4.0) except for aminoglycosides, which were extracted in neutral conditions (7.0~8.0). ${\beta}-Lactams$ showed the highest recoveries in neutral pH due to their degradation characteristics in acidic media. Starta-X, Oasis HLB, and Oasis MCX were frequently applied as an SPE cartridge and Oasis HLB showed the highest recoveries for the majority of antibiotic classes. The homogenized honey and milk were extracted by mixing with acids for deproteinization. Solids and other interfering substances in the extract were eliminated by centrifugation followed by membrane filtration or SPE before injection into HPLC.

• 한국응용과학기술학회지
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• 제29권4호
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• pp.610-617
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• 2012

탱자에는 여러 종류의 monoterpenes, limonoids, flavonoids과 coumarins 등의 구성성분으로 이루어져 있다. 탱자의 구성 성분중에 7-제라닐옥시쿠마린는 7개의 탄소로 구성된 제라닐옥시기의 곁사슬을 가진 물질이다. 그리고 7-제라닐옥시쿠마린은 여러 약리적인 효과들을 나타낸다고 알려져 있다. 본 연구에서는 탱자의 구성성분인 7-제라닐옥시쿠마린과 이들의 다양한 유도체를 합성하였다. 그리고 항염증에 대한 항염증 효능을 알아보기 위하여 염증을 유발하는 일산화질소 억제 cytokine을 측정한 결과는 6-제라닐옥시쿠마린의 성분이 사이토카인인 인터루킨-6가 $1{\mu}M$농도에서 68.9% 를, $10{\mu}M$에서 72.6% 의 저해효과를 나타냈다.