• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.1413-1415
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• 2004

This paper proposes a SVM(Support Vector Machine) training algorithm to control a service robot with voice command. The service robot with a stereo vision system and dual manipulators of four degrees of freedom implements a User-Dependent Voice Control System. The training of SVM algorithm that is one of the statistical learning theories leads to a QP(quadratic programming) problem. In this paper, we present an efficient SVM speech recognition scheme especially based on less learning data comparing with conventional approaches. SVM discriminator decides rejection or acceptance of user's extracted voice features by the MFCC(Mel Frequency Cepstrum Coefficient). Among several SVM kernels, the exponential RBF function gives the best classification and the accurate user recognition. The numerical simulation and the experiment verified the usefulness of the proposed algorithm.

• Structural Engineering and Mechanics
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• 제57권2호
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• pp.327-355
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• 2016

A new analytical derivation of the elastodynamic point load solutions for an isotropic multi-layered half-space is presented by means of the precise integration method (PIM) and the approach of dual vector. The time-harmonic external load is prescribed either on the external boundary or in the interior of the solid medium. Starting with the axisymmetric governing motion equations in a cylindrical coordinate system, a second order ordinary differential matrix equation can be gained by making use of the Hankel integral transform. Employing the technique of dual vector, the second order ordinary differential matrix equation can be simplified into a first-order one. The approach of PIM is implemented to obtain the solutions of the ordinary differential matrix equation in the Hankel integral transform domain. The PIM is a highly accurate algorithm to solve sets of first-order ordinary differential equations and any desired accuracy of the dynamic point load solutions can be achieved. The numerical simulation is based on algebraic matrix operation. As a result, the computational effort is reduced to a great extent and the computation is unconditionally stable. Selected numerical trials are given to validate the accuracy and applicability of the proposed approach. More examples are discussed to portray the dependence of the load-displacement response on the isotropic parameters of the multi-layered media, the depth of external load and the frequency of excitation.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.230-234
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• 2019

Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

• Journal of applied mathematics & informatics
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• 제17권1_2_3호
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• pp.121-144
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• 2005

In this paper, we propose unconstrained and constrained posynomial Geometric Programming (GP) problem with negative or positive integral degree of difficulty. Conventional GP approach has been modified to solve some special type of GP problems. In specific case, when the degree of difficulty is negative, the normality and the orthogonality conditions of the dual program give a system of linear equations. No general solution vector exists for this system of linear equations. But an approximate solution can be determined by the least square and also max-min method. Here, modified form of geometric programming method has been demonstrated and for that purpose necessary theorems have been derived. Finally, these are illustrated by numerical examples and applications.

• Journal of Microbiology and Biotechnology
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• 제18권10호
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• pp.1634-1640
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• 2008

This study was focused on the screening of valuable genetic resources, such as promoters from metagenome, and describes a promoter trapping system with a bidirectional probe concept, which can select promoters or operons from various biological resources including metagenomic DNA. A pair of reporters, GFP and DsRed, facing the opposite direction without promoters, is an effective system that can function regardless of the direction of inserted promoters. The feasibility of this system was tested for the isolation of constitutively expressed promoters in E. coli from a soil metagenome, resulting in a potential pool of various promoters for practical application. The analyses of structural organization of the trapped genes demonstrated that constitutively expressible promoters in E. coli were broadly distributed within the metagenome, and suggested that some promoters were useful for the construction of expression vectors. Based on these observations, three constitutive promoters were employed in the expression vector system and their potentials for practical application were evaluated in terms of expression level, protein solubility, and effects on host growth.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.247-254
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• 2015

In this presentation, I describe the expression and purification of the recombinant liver X receptor β-ligand binding domain proteins in E. coli using a commercially available double cistronic vector, pACYCDuet-1, to express the receptor heterodimer in a single cell as the soluble form. I describe here the expression and characterization of a biologically active heterodimer composed of the liver X receptor β-ligand binding domain and retinoid X receptor α-ligand binding domain. Although many of these proteins were previously seen to be produced in E. coli as insoluble aggregates or "inclusion bodies", I show here that as a form of heterodimer they can be made in soluble forms that are biologically active. This suggests that co-expression of the liver X receptor β-ligand binding domain with its binding partner improves the solubility of the complex and probably assists in their correct folding, thereby functioning as a type of molecular chaperone.

• 한국추진공학회지
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• 제24권4호
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• pp.12-24
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• 2020

이중목 노즐은 유체 추력벡터제어 분야에서 특히 효과적인 방법이며, 다른 축소부가 종래의 축소-확대 노즐의 확대부에 연결된다. 본 연구에서는 3차원 초음속 직사각형 노즐에서 추력벡터제어 성능에 대한 분사각의 영향을 조사하기 위하여 수치해석을 수행하였다. 5개의 분사각에 대하여 다루었으며, 편향각도, 분사 질량유량비, 시스템 전체 추력비, 전체 피칭 추력효율, 대칭면에서의 마하수 분포와 유선 및 다른 면에서 마하수 분포를 포함하는 임계 성능변화가 정량적으로 그리고 정성적으로 분석되었다. 본 연구의 결과는 특히 전투기 설계자에게 유용한 기술적 자료를 제공한다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년 영문 학술대회
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• pp.72-79
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• 2008

The weak point of the artificial neural network(ANN) is that it is easy to fall in local minima when it learns too much nonlinear data. Accordingly, the classification ratio must be low. To overcome this weakness, the hybrid method has been proposed. That is, the ANN learns data selectively after detecting the defect position by the support vector machine(SVM). First, the SVM has been used for determination of the defect position and then the magnitude of the defect has been measured by the ANN. In off-design condition, the operation region of the engine is wide and the nonlinearity of learning data increases. The module system, dividing the whole operating region into reasonably small-size sections, has been suggested to solve this problem. In this study, the proposed algorithm has diagnosed the defects of triple components as well as single and dual components of the gas turbine engine in off-design condition.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.979-982
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• 2006

Two knockout vectors, in which the truncated Kluyveromyces lactis URAS gene is flanked by a direct repeat, were developed for Saccharomyces cerevisiae. Each vector was designed to harbor 5'- and 3'-end homology regions for integration. Two knockout fragments were devised to integrate into the correct locus in a complementary manner to disrupt a gene of interest and. concomitantly to make functional Kl URA3 for transfomant selection. The use of dual complementary knockout cassettes was expected to dramatically reduce integration into unwanted loci in the genome. The knockout system developed in this study was successfully used for disruption of the GAL1 gene in S. cerevisiae.

• Coupled systems mechanics
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• 제10권1호
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• pp.21-38
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• 2021

This work is an attempt to design a dynamic model for a non local bio-thermoelastic medium with diffusion. The system of governing equations are formulated in terms of displacement vector field, chemical potential and the tissue temperature in the context of non local dual phase lag (NL DPL) theories of heat conduction and mass diffusion. Based on this considered model, we study the fundamental solution and propagation of plane harmonic waves in tissues. In order to analyze the behavior of the NL DPL model, we construct basic theorem in the terms of elementary function which determine the existence of three longitudinal and one transverse wave. The effects of various parameters on the characteristics of waves i.e., phase velocity and attenuation coefficients are elaborated by plotting various figures of physical quantities in the later part of the paper.