• The Journal of Korean Society of Virology
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• v.30 no.3
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• pp.171-178
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• 2000

Herpes simplex virus, Varicella zoster virus and Human herpes virus-6 caused central nervous system infections and latent infections but there is no data of the 3 viruses being tested from the same cerebrospinal fluid samples with aseptic meningitis or encephalitis in adults patients. These viruses produced similar neurologic symptoms but difficulties existed in differentiating of etiologic agents and therefore the viruses needed to be detected in the early state. Herpes simplex virus encephalitis (HSVE) in adults, if not treated promptly was fatal. If treated with antiviral drugs in the early phase of encephalitis, neurologic sequales decreased by 65%. Recently, a PCR method for detection of HSVE with CSF was developed. VZV primary and secondary infections caused neurologic symptoms of encephalitis or meningitis. The second frequency of adult encephalitis that caused VZV were reported. HHV-6 caused CNS latent infection that was studied with normal adults brains. But there is no data of HSV, VZV and HHV-6 for aseptic meningitis and encephalitis of Korean adults through etiologic study. We cultured CSFs on HEp-2 cells and simultaneously tested for HSV PCR, VZV nested PCR and HHV-6 PCR with 8 specific primers. The PCR results of CSF from meningitis Korean adults were 13/19 (68.4%) for HSV, 10/19 (52.6%) for VZV and 12/19 (63.2%) for HHV-67/19 (36.8%) cases were triple infected HSV PCR, VZV PCR and HHV-6 PCR positive; 3/19 (15.8%) cases were dual infected HSV PCR and HHV-6 PCR positive; 1119 (0.5%) cases was VZV PCR positive. Strong viral DNA amplification of CSF means a causative virus may be present in aseptic meningitis or encephalitis patients and may cause clinical neurologic symptoms. HSV and HHV-6 viruses detection rate were higher than VZV by PCR with CSFs.

• Journal of Biomedical Engineering Research
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• v.20 no.2
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• pp.173-182
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• 1999

Contrast-enhanced CT has an important role in assessing liver lesions, the optimal protocol to get most effective result is not clear. The mein goal when deciding injention protocol is to optimize lesion detectability with rapid scanning when lesion to liver contrast is maximum. For this purpose, we developed a physiological model of the contrast medium enhancement based on the compartment modeling and pharmacokinetics. Blood supply to liver is achieved in two paths. This dual supply characteristic distinguishes the CT enhancement of liver from that of the other organs. The first path is by hepatic artery and to second, by portal vein. However, it is assumed that only gepatic artery can supply blood to hepatocellular carcinoma(HCC) compartment, thus, the difference of contrast enhancement is resulted between normal liver tissue and hepatic tumor. By solving differential equations for each compartment simultaneously using the computer program Matlab, CT contrast-enhancement curves were simulated. The simulated enhancement curves for aortic, hepatic, portal vein, and HCC compartments were compared with the mean enhancement curves from 24 patients exposed to the same protocols as the simulation. These enhancement curves showed a good agreement. Furthermore, we simulated lesion-to-liver curves for various injection protocols, and the effects were analyzed. The variables to be considered in the injection protocol were injection rate, dose, and concentration of contrast material. These data may help to optimize scanning protocols for better diagnosis.

• Survey Research
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• v.9 no.2
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• pp.119-149
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• 2008

In this changing, complex modern society, as one-person households, dual income households and the elderly population increases and the survey environment gets worse, the past 'method' in which high costs and much time are needed, should face the environmental change. When considering the fact that developed countries in Northern Europe such as Denmark and Finland use administrative data for the Censuses, Korea should carry out further research to use resident registration administrative data in the Registration Census. Based on administrative data, the Registration Census is expected to reduce survey costs and to increase the accuracy and timeliness of surveys. Moreover, a wide variety of statistical demand will be satisfied by producing advanced statistics through the links among administrative data. The paper examines the difference when linking both resident registration administrative data and the results of 2005 Population Census, with a view to improving the Population Census method and preparing for the information age. Also this paper presents some proposals for future Population Censuses. With confidentiality given the top priority, this paper examines the link with matching value of ages and genders at Haeundae-gu, Busan and Boeun-gun, Chungbuk for pragmatic research. Hoenam-myeon, Boeun-gun, Chungbuk marks a low matching rate. Focused on Hoenam-myeon data, this research directly compares the results of 2005 Population Census with resident registration administrative data. Births, deaths, out-migrations and in-migrations from resident registration administrative data as of November 1st 2005 are used especially to increase comparison with the results of 2005 Population Census.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1881-1890
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• 2016

Manganese superoxide dismutase (MnSOD) is a vital enzyme that protects cells from free radicals through eliminating superoxide radicals ($O^{2-}$). Hirudin, a kind of small active peptide molecule, is one of the strongest anticoagulants that can effectively cure thrombus diseases. In this study, we fused Hirudin to the C terminus of human MnSOD with the GGGGS linker to generate a novel dual-feature fusion protein, denoted as hMnSOD-Hirudin. The hMnSOD-Hirudin gene fragment was cloned into the pET15b (SmaI, CIAP) vector, forming a recombinant pET15b-hMnSOD-Hirudin plasmid, and then was transferred into Escherichia coli strain Rosetta-gami for expression. SDS-PAGE was used to detect the fusion protein, which was expected to be about 30 kDa upon IPTG induction. Furthermore, the hMnSOD-Hirudin protein was heavily detected as a soluble form in the supernatant. The purification rate observed after Ni NTA affinity chromatography was above 95%. The hMnSOD-Hirudin protein yield reached 67.25 mg per liter of bacterial culture. The identity of the purified protein was confirmed by western blotting. The hMnSOD-Hirudin protein activity assay evinced that the antioxidation activity of the hMnSOD-Hirudin protein obtained was $2,444.0{\pm}96.0U/mg$, and the anticoagulant activity of the hMnSOD-Hirudin protein was $599.0{\pm}35.0ATU/mg$. In addition, in vitro bioactivity assay showed that the hMnSOD-Hirudin protein had no or little cytotoxicity in H9c2, HK-2, and H9 (human $CD_4{^+}$, T cell) cell lines. Transwell migration assay and invasion assay showed that the hMnSOD-Hirudin protein could suppress human lung cancer 95-D cell metastasis and invasion in vitro.

• Korean Journal of Bronchoesophagology
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• v.16 no.2
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• pp.145-148
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• 2010

Background: Laryngeal papillomatosis, which is cased by human papillomavirus, is the most common benign neoplasm of the larynx. However, the prognosis of this disease remains really unpredictable. The aim of this paper is to determine whether any clinical features at the time of diagnosis could predict its course. Material and Method: Eighty-six patients treated at our institution during the last 20 years were analyzed retrospectively. All patients had microsurgery under general anesthesia. All patients' follow-up period was more than 1 year. We divided the patients into 1) Juvenile versus adult group based on their age 20, 2) single surgical method with laser versus dual surgical method with laser and microdebrider group, and 3) single subsite versus multiple subsites group. And we compared the therapeutic outcome. Results: The recurrence rate was 100% (15 patients) in the JP group and 56% (40 patients) in the AP group. Juvenile versus adult group was the only independent prognostic factor by univariate, and multivariate analysis. Microdebrider resection technique and multiple subsites were not associated with treatment result. Time period from the first surgery to recurrence detection was different statistically only for the age group. The number of surgery in the JP group ranged from 1 to 31 (mean 8.8). In the AP group the number of surgery ranged from 1 to 25 (mean 3.7). It was statistically different. Conclusion: Prognosis for the laryngeal papillomatosis remains unpredictable. Only patients' age was the only independent prognostic factor.

• Korean Journal of Comparative Education
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• v.27 no.1
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• pp.247-284
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• 2017

The purpose of this study is to analyze firm's motivation of participating and investing in apprenticeship in Germany and Korea, and to investigate institutional factors influencing firm's motivation. By comparing institutional factors of the two countries, it aims to drawing out policy implications for improving Korean apprenticeship. The main method for data collection was comprehensive literature review on international organizations, each countries' government and research institutes' policy materials, statistical data, research outputs and media resources related to each countries' apprenticeship. Considering whether firm's motivation for participating and investing in apprenticeship is production-oriented or investment-oriented, Germany is more inclined to investment motivation with firm's covering net cost during apprenticeship period. On the other hand, Korea is more inclined toward production orientation with firm's expectation of gaining net profit during the training period. Why is firm's training motivation different in these two countries? The author tried to find the reason from the difference of institutional factors of the countries by dividing institutional factors into 4 categories: context(tripartite relations, legal framework), input (flexibility of the system, government incentive), process(training contents, training duration, quality assurance), and output(completion/retention rate, apprentice's productivity). The key implication from the comparative analysis of institutional factors is that it is necessary to enforce companies to have "accountability" on the minimum critical elements, but also to ensure them to have "autonomy" on the rest of the elements.

• Journal of the Korean Society of Hazard Mitigation
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• v.11 no.3
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• pp.209-218
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• 2011

The present study proposes a new estimation relationship for the transmission rate of the steel breakwater which is expected to make up for the weakness points in existing hard solution for shore protection. The steel breakwater consists of the wave dissipator of the dual horizontal plates, the supporting columns and their foundations, and thus its respective designs should also be conducted one by one. Furthermore, the breakwater has to ensure both functions of shore protection and structure stabilization. The study produced experimental data for the stability and safety investigation of the steel breakwater. The forces acting on the steel breakwater were classified into two categories, one is vertical up and down loads for the pile resistance and the other was maximum difference of the vertical load acting on horizontally different position for the torsion. The study applied the stability force produced by the summation of maximum pressure at each point and the safety force acting on each point simultaneously. The regular wave corresponding to the significant wave was utilized for measuring wave pressure and force. The study showed the method for the proper position of submerged upper plate by considering occurrence frequency of tide level. The design process finally determined by trial and error is proposed in the present study.

• Korean Journal of Social Welfare Studies
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• v.47 no.3
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• pp.35-60
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• 2016

This study analyzes the unequal distributional effect of threesome of family policy(child benefit, childcare services and parental leave) focusing on family income, mother's status in labor market in Korea. To measure the unequal distributional effect of family policies, this study used the quantile analysis. The results of this study are as follows. First, in terms of childcare service and parental leave, there is some difference of the rate of use by family income and mother's status in labor market. Second, total public fund for childcare services, child benefits and parental leave are high in fourth income quintile, and mothers work regularly. Third, public fund is high in fourth income quintile, dual earners, mothers work regularly, the family has many number of child, and is high educational level of parents. Finally, the results of quantile regression show the biggest factors of unequal distribution of family policy are mother's stable work and it deepens the inequalities and differences. Based on these results, this article suggests that more equal right to access and use family policy regardless of the type of employment, adequate minimum income through income transfer, and universal application of the policy.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.419-425
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• 2019

The objective of this study was to investigate the cooling performance characteristics of a hybrid refrigeration system in a heavy duty vehicle. The tested hybrid refrigeration system had additionally an electric compressor besides the present mechanical compressor for selective use according to the operating conditions. The applied electric compressor was a scroll type and with 18.0 cc displacement. In order to analyze the performance characteristics of the hybrid refrigeration system with respect to the cooling capacity and Coefficient of Performance (COP), other components, including two different types of compressors, were installed and tested under various operating conditions such as compressor speed and air flow rate of the evaporator. When the electric compressor was operated at 4,500 rev/min, the cooling capacity was about 4.0kW and COP was 3.5. When the mechanical compressor was operated, whereas the cooling capacity was higher than the electric controlled compressor, COP was lower due to the larger displacement and higher power consumption. To analyze the hybrid system operating characteristics due to reasonable cooling capacity with electric compressor operation, the mechanical compressor and electric compressor were operated by turns every 10 minutes under certain system operating conditions. Because surge pressure occurred when both compressors were switched on, the operating strategy required some time to balance the system pressure.

• Clean Technology
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• v.25 no.3
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• pp.263-272
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• 2019

Air pollutants have a high impact on everyday life as well as on human health; therefore, new technologies such as low-emission vehicles and add-on systems for air pollutant reduction are needed for our society. However, the environmental benefits and costs of those technologies are not taken into account in existing economic feasibility assessments, which is a barrier that needs to be overcome for green technology to achieve wide dissemination and fast penetration in the market. Thus, this study develops a methodology to assess the economic feasibility of an air pollutant reduction technology by taking into account the social costs from air pollutants and carries out a case study to validate the methodology. Because the social unit costs for air pollutants have not been evaluated yet in South Korea, the methodology uses the social unit costs evaluated for the European Union that are then converted to those for South Korea based on the measuring criteria for vehicle emission gases, parity purchasing price, foreign currency exchange rate, and customer price index. The social unit costs for South Korea are used to assess economic feasibility. A case study was performed to assess the economic feasibility of a dual fuel system using diesel and compressed natural gas by taking into account social costs from air pollutants as well as economic costs. This study could contribute to assessing the true economic feasibility of green technology, projects, and policy related with air pollutant reduction.