• Nuclear Medicine and Molecular Imaging
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• v.41 no.3
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• pp.226-233
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• 2007

Purpose: Dual reporter gene imaging has several advantages for more sophisticated molecular imaging studies such as gene therapy monitoring. Herein, we have constructed hepatoma cell line expressing dual reporter genes of sodium iodide symporter (NIS) and enhanced green fluorescence protein (EGFP), and the functionalities of the genes were evaluated in vivo by nuclear and optical imaging. Materials and Methods: A pRetro-PN vector was constructed after separating NIS gene from pcDNA-NIS. RSV-EGFP-WPRE fragment separated from pLNRGW was cloned into pRetro-PN vector. The final vector expressing dual reporter genes was named pRetro-PNRGW. A human hepatoma (HepG2) cells were transfected by the retrovirus containing NIS and EGFP gene (HepG2-NE). Expression of NIS gene was confirmed by RT-PCR, radioiodine uptake and efflux studies. Expression of EGFP was confirmed by RT-PCR and fluorescence microscope. The HepG2 and HepG2-NE cells were implanted in shoulder and hindlimb of nude mice, then fluorescence image, gamma camera image and I-124 microPET image were undertaken. Results: The HepG2-NE cell was successfully constructed. RT-PCR showed NIS and EGFP mRNA expression. About 50% of cells showed fluorescence. The iodine uptake of NIS-expressed cells was about 9 times higher than control. In efflux study, $T_{1/2}$ of HepG2-NE cells was 9 min. HepG2-NE xenograft showed high signal-to-background fluorescent spots and higher iodine-uptake compared to those of HepG2 xenograft. Conclusion: A hepatoma cell line expressing NIS and EGFP dual reporter genes was successfully constructed and could be used as a potential either by therapeutic gene or imaging reporter gene.

• Current Optics and Photonics
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• v.2 no.2
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• pp.134-139
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• 2018

A novel approach for frequency-tripling vector signal generation via balanced detection without precoding and optical filter is proposed. The scheme is mainly utilizing an integrated dual-polarization quadrature phase shift keying (DPQPSK) modulator. In the DPQPSK modulator, one QPSK modulator is driven by an RF signal to generate high-order optical sidebands, while the other QPSK modulator is modulated by I/Q data streams to produce baseband vector signal as an optical carrier. After that, a frequency-tripling 16-quadrature-amplitude-modulation (16QAM) vector millimeter-wave (mm-wave) signal can be obtained by balanced detection. The proposed scheme can reduce the complexity of transmitter digital signal processing. The results show that, a 4 Gbaud baseband 16QAM vector signal can be generated at 30 GHz by frequency-tripling. After 10 km single-mode fiber (SMF) transmission, the constellation and eye diagrams of the generated vector signal perform well and a bit-error-rate (BER) below than 1e-3 can be achieved.

• East Asian mathematical journal
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• v.33 no.1
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• pp.83-102
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• 2017

The main purpose of this paper is to introduce a pair new class of primal and dual problem associated with an operator. We prove the sufficient optimality theorem, weak duality theorem and strong duality theorem for these problems. The equivalence between the generalized optimization problems and the generalized variational inequality problems is studied in ordered topological vector space modeled in Hilbert spaces. We introduce the concept of partial differential associated (PDA)-operator, PDA-vector function and PDA-antisymmetric function to show the existence of a new class of function called, ($T_{\eta};{\xi}_{\theta}$)-invex functions. We discuss first and second kind of ($T_{\eta};{\xi}_{\theta}$)-invex functions and establish their existence theorems in ordered topological vector spaces.

• Korean Journal of Mathematics
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• v.15 no.1
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• pp.13-26
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• 2007

We prove that every strong null sequence in a Banach space X lies inside the range of a vector measure of bounded variation if and only if the condition $\mathcal{N}_1(X,{\ell}_1)={\Pi}_1(X,{\ell}_1)$ holds. We also prove that for $1{\leq}p<{\infty}$ every strong ${\ell}_p$ sequence in a Banach space X lies inside the range of an X-valued measure of bounded variation if and only if the identity operator of the dual Banach space $X^*$ is ($p^{\prime}$,1)-summing, where $p^{\prime}$ is the conjugate exponent of $p$. Finally we prove that a Banach space X has the property that any sequence lying in the range of an X-valued measure actually lies in the range of a vector measure of bounded variation if and only if the condition ${\Pi}_1(X,{\ell}_1)={\Pi}_2(X,{\ell}_1)$ holds.

• Journal of the Korean Society of Propulsion Engineers
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• v.14 no.5
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• pp.1-7
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• 2010

Dual throat nozzle(DTN) is recently attracting much attention as a new concept of the thrust vectoring technique. This DTN is designed with two throats, an upstream minimum and a downstream minimum at the nozzle exit, with a cavity in between the upstream throat and exit. In the present study, a computational work has been carried out to analyze a fundamental performance of a dual throat nozzle(DTN) at various nozzle pressure ratios(NPR) and throat area ratios. Two-dimensional, axisymmetric, steady, compressible Navier-Stokes equations were solved using a fully implicit finite volume scheme. NPR was varied in the range of NPR from 2.0 to 10.0, at different throat area ratios. The present computational results were validated with some experimental data available. Based upon the present results, the performance of DTN is discussed in terms of the discharge coefficient and thrust efficiency.

• The Transactions of the Korean Institute of Power Electronics
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• v.27 no.2
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• pp.126-133
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• 2022

This study proposes a suppression of zero sequence current (ZSC), which is caused by zero sequence voltage (ZSV) for a dual two-level inverter with single DC bus. Large output voltages enable the dual inverter with single DC bus to improve a system efficiency compared with single inverter. However, the structure of dual inverter with single DC bus inevitably generates ZSC, which reduces the system efficiency and causes a current ripple. ZSV is also produced by dead time, and its magnitude is determined by the DC bus and current direction. This study presents a novel space vector modulation method that allows the instantaneous suppression of ZSC. Based on a condition where a switching period is twice a sampling (control) period, the proposed control method is implemented by injecting the offset voltage at the primary inverter. This offset voltage is injected in half of the switching period to suppress the ZSC. Simulation and experiments are used to compare the proposed and conventional methods to determine the ZSC suppression performance.

• Proceedings of the KIEE Conference
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• 1991.11a
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• pp.175-178
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• 1991

The drive system of servo motor is requested to have robustness of disturbance and parameter variation. However, the dynamics of PMSM drive change significantly by forced disturbance and parameter variation. Moreover, the state error caused by them should be suppressed completely and rapidly. In this paper, the vector-control system of PMSM using dual adaptive control loop is investigated. In the proposed system, linear adaptive control loop rapidly recovers the state error caused by both disturbance and parameter variation. In the dual adaptive control loop, the inner loop reduces the system sensitivity of parameter variation and disturbance, and the outer loop suppresses the state error caused by them completely. The proposed servo system is verified through a computer simulations and experimental results.

• Journal of Biomedical Engineering Research
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• v.8 no.2
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• pp.171-176
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• 1987

Wiener filtering method was applied to the dual energy imaging procedure in digital radiography(D.R.). A linear scanning photodiode arrays with 1024 elements(0.6mm H 1.3mm pixel size) were used to obtain chest images in 0.7 sec. For high energy image acquisition, X-ray tube was set at 140KVp, 100mA with a rare-earth phosphor screen. Low energy image was obtained with X-ray tube setting at 70KVp, 150mA. These measured dual energy images are represented in the vector matrix notation as a linear discrete model including the additive random noise. Then, the object images are restored in the minimum mean square error sense using Wiener filtering method in the transformed domain. These restored high and low energy images are used for computation of the basis image decomposition. Then the basis images are linearly combined to produce bone or tissue selective images. Using this process, we could improve the signal to noise ratio characteristics in the material selective images.

• East Asian mathematical journal
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• v.5 no.2
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• pp.209-213
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• 1989

The efficiency(Pareto optimum) is a type of solutions for multiobjective programs. We formulate duality relations for multiobjective nonlinear programs by using the concept of efficiency. The results are the weak and strong duality relations for a vector dual of the Wolfe type involving invex functions.

• Journal of the Chungcheong Mathematical Society
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• v.36 no.3
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• pp.163-169
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• 2023

In this article, we consider a vectorial linear connection which is determined by three fixed vector fields. Classifying these vectorial connections, we obtain a new type of generalized statistical manifolds which allow non-zero torsion.