• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.94-101
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• 2002

Two experiments were conducted to evaluate the feeding value of rancid rice bran in finishing pigs. In exp. 1, fresh (FRB), rancid (RRB), pelleted and extruded rice bran were used to determine stability and nutrient digestibility. The free fatty acid (FFA) values of FRB and RRB were 8.2 and 15.3%, respectively. Some of the FRB was pelleted ($70^{\circ}C$) or extruded ($110^{\circ}C$). In exp. 2, a total of 48 pigs ($Landrace{\times}Yorkshire{\times}Duroc$, $51.12{\times}0.5kg$) were employed for a 56-d feeding trial with 3 treatments: Control (defatted rice bran+animal fat), 20% FRB (8.2% FFA), and 20% RRB (15.6% FFA). There was a significant difference (p<0.05) in FFA% between raw and pelleted, and extruded rice bran on d 10 after storage. On d 30 the extruded rice bran showed lower (p<0.05) FFA% than the pelleted one. Dry matter digestibility was higher (p<0.05) in processed rice brans (pelleted or extruded) than raw rice bran (FRB or RRB). Energy and protein digestibilities in extruded rice bran were higher (p<0.05) than those in raw rice brans. The digestibilities of isoleucine, leucine and phenylalanine were lower (p<0.05) in RRB than FRB. Pigs fed diets containing FRB grew faster (p<0.05) and showed better feed conversion ratio (p<0.05) than those fed diets containing defatted rice bran or RRB. Carcass characteristics including dressing percentage and backfat thickness were not affected (p>0.05) by dietary treatments. With increasing storage time, the raw pork from RRB showed higher (p<0.05) thiobarbituric acid reactive substance (TBARS) and peroxide value (POV) than those from FRB when stored at $1^{\circ}C$ for 3 weeks. Cooked pork showed rapid increase in TBARS and POV as compared to raw pork regardless of rice bran rancidity. As the storage time passed, Lightness (L) was lower (p<0.05) in RRB than FRB. Redness (a) was higher (p<0.05) in control than rice bran groups when stored 2-3 weeks. However, there was no difference (p>0.05) in redness (a) between the two rice bran groups. In conclusion, feeding rancid rice bran gave negative effects on growth performance and pork quality in finishing pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.555-564
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• 2002

Twenty Landrace and twenty Min piglets, with an average initial body weight of 22.4 kg, were randomly divided into 5 groups with 4 animals per group, within each of the breeds. The piglets were housed in individual concrete pens. Each group of the piglets was fed one of 5 diets. The diets contained either 20% raw Argentine soybeans, 20% processed Argentine soybeans ($118^{\circ}C$ for 7.5 min.), 20% raw Chinese soybeans, 20% processed Chinese soybeans ($118^{\circ}C$ for 7.5 min.) or no soybean products (control diet). Faecal samples were collected on days 6, 7 and 8 of the treatment period. Digestibilities of dietary nutrients were determined with AIA (acid insoluble ash) as a marker. After a 17 day treatment, three piglets were killed from each of the groups. Tissue samples of small and large intestine for light and electron microscopy examination were taken immediately after the opening of abdomen. Then, the weight or size of relevant organs was measured. The results show that the digestibilities of dry matter (DM), crude protein (CP) and fat were higher in Min piglets than in Landrace piglets (p<0.05). The diets containing processed soybeans had a significant higher CP digestibility than the control diet and the diets containing raw soybeans (p<0.05). Landrace piglets had heavier and longer small intestines, heavier kidneys and a lighter spleen than Min piglets (p<0.05). The pancreas of the animals fed the diets containing processed soybeans was heavier than that of the animals fed control diet (p<0.05) and the diets containing raw soybeans. But, the differences between raw and processed soybean diets were not significant. A significant interaction (p<0.05) between diet and pig breed was observed in weight of the small intestine. The Landrace piglets increased the weight in their small intestine when they were fed the diets containing soybeans. In the light micrographs and electron scanning micrographs, it was found that the villi of small intestinal epithelium of animals (especially Landrace piglets) fed the diets containing raw Chinese soybeans were seriously damaged. The transmission electron micrograph showed that a lot of vesicles were located between the small intestinal microvilli of these piglets. The histological examination also indicated that the proportion of goblet cells in villi and crypts in the piglets consuming the control diet was significantly lower (p<0.01 and p<0.02, respectively) than those of the animals consuming the diets containing raw or processed soybeans.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1659-1666
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• 2000

Two experiments were conducted to determine the effects of Leucaena leucocephala (leucaena) and Gliricidia sepium (gliricidia) supplementation on intake, digestion, outflow rates, microbial protein yield and live-weight changes in sheep fed with ammoniated rice straw (ARS). In experiment 1, three rumen cannulated Japanese Corriedale wether (mean body weight of 35.6 kg) in $3{\times}3$ Latin Square Design were used. Animals were fed ad libitum ARS alone, or supplemented with 200 g of either leucaena or gliricidia. In experiment 2, twenty-four growing native Philippine sheep with average body weight of $13.5{\pm}0.25kg$ were used in a completely randomized design (CRD) and offered similar diets to those of experiment 1. Supplementation increased total dry matter intake and nutrient digestibility except for fiber (p<0.05) without affecting ARS consumption. Nitrogen balance revealed that absorbed and retained N was significantly higher in leucaena and gliricidia. The significant improvement in N utilization and more digestible OM intake brought about by the inclusion of leucaena and gliricidia to ARS resulted in increased (p<0.05) microbial N yield. Efficiency of microbial N supply in supplemented group was not significantly different, but higher (p<0.05) than the 24.92 g N/kg DOMR for ARS group. Liquid outflow rate was 7.8 and 6.8 %/h, while the solid phase of rumen digesta was 4.4 and 3.8 %/h for the leucaena and gliricidia group respectively, which were significantly higher than 5.30 and 2.50 %/h in the control diet. The increase in total DMI resulted to higher (p<0.01) growth performance and efficient feed utilization. Average daily gain (ADG) was 19.3, 34.6 and 33.9 g/d for the ARS, leucaena and gliricidia respectively. It is therefore concluded that addition of leucaena and gliricidia to ARS in could increase nutrient intake and digestibility, subsequently improving N utilization and livestock performance.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.645-652
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• 2000

A total of 80 piglets ($5.18{\pm}0.61kg$ of BW; 16 d of age) were fed experimental diets to evaluate the effect of extruded full-fat soybean (EFS) on the growth of eatly weaned pigs. Pigs were allotted into five treatments based on body weight, in a completely randomized block design. Each treatment has 4 replicates of 4 animals each. Treatments consisted of diets representing substitutional ratios of EFS for soybean meal. 1) 100:0 (SBM), 2) 75:25 (EFS 25), 3) 50:50 (EFS 50), 4) 25:75 (EFS 75) and 5) 0:100 (EFS 100). During phase I (d 0 to 7), piglets were fed diets containing 3,340 kcal ME, 26% crude protein, 1.85% lysine, 1.0% Ca and 0.9% P. For phase II (d 8 to 21), piglets were fed diets supplying 3,340 kcal ME, 23% crude protein, 1.65% lysine, 0.9% Ca and 0.8% P. Urease activity (pH rise) of EFS (0.18) was three times higher than that of SBM (0.06) indicating that processing conditions were not efficient enough to inactivate urease activity. During the first week postweaning, pigs fed SBM had significantly greater average daily gain (ADG), average daily feed intake (ADFI) and better feed conversion ratio (FCR) compared to pigs fed FFS diets. Linear negative effect on growth rate was found as the inclusion rate of FFS increased. During d 8 - 21 postweaning, piglets fed EFS 50 diet showed the best ADG and FCR despite no significant difference between treatment SBM and EFS 25 have been observed. Overall, piglets fed diets up to 50% FFS inclusion rate exhibited similar weight gain. Only piglets fed EFS 100 diet showed a significantly decreased growth rate. No other significant effect was found in feed intake and feed conversion ratio. At d 7, dry matter digestibility was higher in pigs fed SBM diet than piglets fed EFS 75 diet (p<0.05) and crude protein digestibility was higher in piglets fed SBM diet than piglets fed EFS 50, EFS 75 and EFS 100 (p<0.05). At d 21, no difference other than in phosphorus digestibility was detected. This indicates that piglets at 21 d postweaning are capable of utilizing nutrients from FFS. No treatment effects were detected in blood metabolites. The data suggests that piglets at 16 d of age are not sufficiently mature to use extruded FFS in their diets. Nevertheless, FFS seemed to be able to replace upto 50% of SBM in weaned piglet diet.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.10
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• pp.1455-1463
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• 2006

Effects of different levels of molasses in a concentrate supplement on milk yield of cows grazing Setaria grass pastures were investigated. Thirty Friesian cows, 6-7 years old with mean pre-experimental body weight of $428{\pm}6.5$ kg, in early stage of lactation were randomly allotted to five dietary treatments in a completely randomized design experiment that lasted for 126 days. Experimental dietary treatments were forage alone and forage/concentrate mixtures with molasses included at 0, 5, 10 or 15% levels designated as $T_1$, $T_2$, $T_3$, $T_4$ and $T_5$, respectively. The parameters studied were voluntary dry matter (DM) intake, average daily live weight change (LWC), milk yield, body condition score (BCS) and apparent nutrient digestibility coefficients. The DM and energy contents of Setaria grass were low compared to the concentrate diets. Fibre fractions-NDF, ADF, ADL, hemicellulose and cellulose; and gross energy were higher in concentrate mixtures than in the forage. Total DM intake (forage+concentrate mixtures) was significantly higher (p<0.001) in cows on the concentrate mixtures. LWC was not significantly different (p>0.05) between the cows. Average milk yields were significantly different (p<0.05) between cows. Fat corrected milk (FCM) was similar among cows in the treatments. BCS was better (p<0.001) in cows on concentrate mixtures. Digestibilities of DM, CP, NDF, ADF, ADL, OM, and energy were significantly higher (p<0.001) in cows on $T_2$, $T_3$, $T_4$ and $T_5$ than in those on $T_1$. There were no significant differences in the digestibility of DM, CP, NDF, ADF and ADL (p>0.001) in cows on concentrate mixtures. This study therefore demonstrated that lactating dairy cows in Fiji need a level of readily fermented energy source such as molasses in their diets; however, a level above 10% is not nutritionally suitable for lactating dairy cows. Based on data on production parameters-milk yield, fat corrected milk, body condition score and apparent nutrient digestibility coefficients-molasses levels that range between 5-10% are recommended, however, 10% is the best and therefore recommended for inclusion in the concentrate mixture of lactating dairy cows on a basal diet of Setaria sphacelata in Fiji.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.4
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• pp.393-402
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• 1995

Experiments were conducted to evaluate the nutritive values of supplemental L-lysine, liquid and powder type, and DL-methionine in weanling pigs. For feeding trial, 165 weanling pigs were treated in 2 controls; 18 and 16% CP, 6 supplementations of lysine alone to 16% CP diets; 0.1, 0.2 and 0.4% of liquid and powder type each, and 3 supplementations of lysine + methionine to 15% CP diets; 0.05 + 0.025, 0.1 + 0.05 and 0.2 + 0.1%. Pigs were fed for 5 week to investigate the protein sparing effect of supplemental amino acid, and the optimal supplemental level. A metabolic trial included the measurements of digestibilities of dry matter, crude protein, crude fat, crude fiber, energy, phosphorus and amino acids. The liver acinar cell culture was conducted for the protein synthesis activity of the pigs fed each experimental diet. Supplementation of both type of L-lysine in 16% CP diet showed improved daily weight gain and feed efficiency which were compatible with those of pigs fed 18% CP diet. Groups fed liquid lysine did not differ from those fed powder type in growth performance. Supplementation of lysine and methionine to 15% CP diet did not improve growth performance of pigs to the extent that 18% CP diet was fed. In nutrient digestibility, 16% CP control diet showed significantly (p < 0.05) lower crude protein digestibility than any other treatments. Digestibilities of 16% CP diets with lysine supplementation were equal to that of 18% CP control, while digestibilities of 15% CP diets with the supplementation of lysine + methionine was inferior to that of 18% CP control. Supplementation of lysine alone reduced the nitrogen excretion compared to the none supplemented control groups. However, addition of lysine + methionine excreted more nitrogen than controls. Pigs fed diet supplemented with lysine alone, or lysine + methionine excreted less fecal phosphorus than those fed none supplemetation. Retained protein from liver tissue of pigs fed 18% diet was significantly (p < 0.05) greater than those fed 16% CP diet. A significant difference (p < 0.05) was observed in physical type of lysine. Feeding of powder type showed less secreted protein and greater retained protein in the culture of liver acinar cell. It is concluded that supplementation of lysine at the level of 0.1 to 0.2% can spare 2% of dietary protein and reduce nitrogen excretion by 19.3%. Also, no difference in nutritional values was observed between liquid and powder lysine in weanling pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.4
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• pp.475-478
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• 2005

This study was conducted to evaluate feeding value of berseem and lucerne silage as a replacement for conventional fodder (berseem fodder) in lactating Nili buffaloes. Fifteen early lactating multi-parous Nili buffaloes, five buffaloes in each group were allotted three experimental diets. Berseem and lucerne fodders were ensiled at 30% DM (wheat straw was used to adjust the DM of fodders) with molasses (at the rate of 2% of fodder DM) in two bunker silos for 30 days. The diets contained 75% DM from berseem fodder (BF), 75% DM from berseem silage (BS) and 75% DM from lucerne silage (LS). Each diet contained 25% concentrate DM. Diets were mixed daily and fed twice a day at ad libitum intakes. Dry matter intake (DMI) was significantly higher (13.8 kg/d) in buffaloes fed BF diet than those fed LS (12.5 kg/d) and BS (11.9 kg/day) diets. The differences in digestible DMI and DMI as percent body weight were significant between fodder and silage based diets but non-significant when BS and LS were compared. Lower DMI with silage-based diets was probably because of low silage pH. Intake of NDF (NDFI) was higher (5.68 kg/d) in buffaloes fed BF diet followed by those fed LS (5.50 kg/d) and BS (5.00 kg/d) diets. The difference was significant (p<0.05) across fodder and silage based diets but NDFI was non-significant across both silage-based diets. The apparent DM digestibility was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Four percent fat corrected milk yield was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Higher milk yield with fodder based diet was because of more digestible nutrient intake (Table 3) compared with silage based diets. Milk CP, TP and NPN and SNF did not show any treatment effects. The present results indicated that the berseem and lucerne fodder ensiled at 30% DM level with 2% molasses could safely replace (75% DM) the conventional leguminous fodder in the diets of lactating Nili buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.2
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• pp.266-273
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• 2002

A total of 225 day-old broiler chicks (43.08 g initial body weight) were allotted to three dietary treatments for a 6-week feeding trial. The treatments were 1) Control (defatted rice bran; DFRB), 2) fresh rice bran (FRB) and 3) rancid rice bran (RRB). Rice brans were intentionally spoiled by two degrees of rancidity by the values of free fatty acids (FFA): 7.6% (FRB) and 16.3% (RRB). Diets were prepared on an isonutrient basis, and defatted or rancid rice brans were included 5 and 10% for starter (0-3 week) and finisher (3-6 week), respectively. At the end of the feeding trial, six chicks per treatment were sacrificed, and thigh meats were ground and stored at $1^{\circ}C$ for thiobarbituric acid reactive substances (TBARS) and peroxide value (POV) analyses. For a digestibility, 48 growing chicks (4 weeks old) were employed in cages (3 replicates/treatment, 2 birds/cage) according to the experimental design: FRB, RRB, pelleted and extruded rice bran. Some of the FRB were pelleted ($70^{\circ}C$) or extruded ($110^{\circ}C$). There was no significant difference in growth performance during the starter period, but chicks fed a diet containing DFRB grew faster (p<0.05) with increased feed intake (p<0.05) than those fed diets containing rice brans, FRB or RRB, during the finisher period. Feed conversion ratio in the RRB was inferior (p<0.05) to the DFRB. Between rice bran groups, weight gain was higher (pco.os) in FRB than in RRB during finisher period. There was a similar trend in growth performance of chicks for the overall period (0-6 week) as the finisher period. Dry matter and energy digestibilities were higher (p<0.05) in extruded than in RRB group. Protein digestibility was improved (p<0.05) when rice bran was extruded, but not pelleted. The chicken meats from RRB showed higher (p<0.05) TBARS than those from FRB during storage for 4 weeks at $1^{\circ}C$. In conclusion, it would appear that feeding rancid rice bran gave negative effects on growth performance and lipid stability of meat in broiler chicks.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.8
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• pp.1164-1170
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• 2005

This study was conducted to evaluate performance, breast (thigh) muscle yield, nutrient digestibility and serum biochemical parameters of broilers fed low-protein diets supplemented with various threonine to lysine ratios (Thr/Lys). Three hundred and twenty four day-old male Arbor Acres broilers were randomly allotted to six dietary treatments with six replicates per treatment and nine broilers per replicate. Six treatments included one control diet [formulated according to NRC (1994)], and five experimental diets (treatments 1-5). In treatments 1-5, the crude protein level was 2% lower than that of the NRC control diet. The Thr/Lys in treatments 1-5 was 0.65, 0.70, 0.75, 0.80 and 0.85 during the starter and grower phases and 0.70, 0.75, 0.80, 0.85 and 0.90 during the finisher phase with other nutrient levels kept consistent. The results showed that: (1) With increasing Thr/Lys, ADG of treatments 1, 3 and 4 were lower (p<0.05) than those of the NRC control diet during the starter phase; ADG of treatments 1-5 were lower (p<0.05) than those of the NRC control during the grower phase, and ADG of treatment 1 was lower (p<0.05) than that of the NRC control during the finisher phase. Average daily feed intake in treatments 1 and 4 were lower (p<0.05) than that of broilers on the NRC control diet during the grower phase. Feed/gain (F/G) of the NRC control diet was lowest (p<0.05) during the starter phase. F/G in treatments 2 and 5 were higher (p<0.05) than that of NRC during the grower phase. (2) Breast muscle proportion on d 21 increased linearly (p<0.05) in response to graded levels of Thr/Lys. (3) No differences were detected for dry matter, energy and crude protein digestibility among all seven treatments. (4) On d 21, serum triglyceride of broilers on dietary treatments 3 and 5 was higher (p<0.05) than that of broilers in control. The results indicate that the low-protein diets supplemented with an appropriate Thr/Lys could not support the same performance that was achieved by the broilers fed the NRC control diet.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.4
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• pp.544-550
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• 2015

This study was conducted to investigate effects of heat-oxidized soy protein isolate (HSPI) on growth performance, serum biochemical indices, apparent nutrient digestibility and digestive function of broiler chickens. A total of 320 1-day-old Arbor Acres chicks were randomly divided into 4 groups with 8 replicates of 10 birds, receiving diets containing soy protein isolate (SPI, control group) or the same amount of SPI heated in an oven at $100^{\circ}C$ for 1, 4, or 8 h, for 21 days, respectively. The results indicated that compared with the control group, body weight gain and feed intake of birds fed diet containing SPI heated for 8 h were significantly lower (p<0.05). Serum urea nitrogen concentration was higher in the broilers fed diet containing SPI heated for 4 or 8 h at d 21 (p<0.05). In contrast, serum glucose content was decreased by HSPI substitution at d 21 (p<0.05). The relative pancreas weight in HSPI groups was higher than that in the control group at d 21 (p<0.05). Meanwhile, the opposite effect was observed for relative weight of anterior intestine and ileum in broilers fed a diet containing SPI heated for 8 h (p<0.05). Birds fed diets containing SPI heated for 4 or 8 h had a decreased lipase activity in anterior intestinal content at d 14 and 21 (p<0.05), respectively. In addition, the same effect was also noted in broilers given diets containing SPI heated for 1 h at d 21 (p<0.05). Similarly, amylase, protease and trypsin activity in anterior intestinal content were lower in broilers fed diets containing SPI heated for 8 h at d 21 (p<0.05). The apparent digestibility of dry matter (DM) from d 8 to 10 and DM, crude protein (CP), and ether extract from d 15 to 17 were lower in broilers fed diets containing SPI heated for 8 h (p<0.05). Besides, birds given diets containing SPI heated for 4 h also exhibited lower CP apparent digestibility from d 15 to 17 (p<0.05). It was concluded that HSPI inclusion can exert a negative influence on the growth performance of broilers, which was likely to result from the simultaneously compromised digestive function.