Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The Effects of Chinese and Argentine Soybeans on Nutrient Digestibility and Organ Morphology in Landrace and Chinese Min Pigs

  • Qin, G.X. (Faculty of Animal Science and Technology, Jilin Agricultural University) ;
  • Xu, L.M. (Department of Animal Science and Tech., Northeast Agricultural University) ;
  • Jiang, H.L. (Faculty of Animal Science and Technology, Jilin Agricultural University) ;
  • van der Poel, A.F.B. (Department of Animal Nutrition, Wageningen Institute of Animal Sciences (WIAS), Wageningen Agricultural University) ;
  • Bosch, M.W. (Department of Animal Nutrition, Wageningen Institute of Animal Sciences (WIAS), Wageningen Agricultural University) ;
  • Verstegen, M.W.A. (Department of Animal Nutrition, Wageningen Institute of Animal Sciences (WIAS), Wageningen Agricultural University)
  • Received : 2001.05.08
  • Accepted : 2001.11.15
  • Published : 2002.04.01
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Abstract

Twenty Landrace and twenty Min piglets, with an average initial body weight of 22.4 kg, were randomly divided into 5 groups with 4 animals per group, within each of the breeds. The piglets were housed in individual concrete pens. Each group of the piglets was fed one of 5 diets. The diets contained either 20% raw Argentine soybeans, 20% processed Argentine soybeans ($118^{\circ}C$ for 7.5 min.), 20% raw Chinese soybeans, 20% processed Chinese soybeans ($118^{\circ}C$ for 7.5 min.) or no soybean products (control diet). Faecal samples were collected on days 6, 7 and 8 of the treatment period. Digestibilities of dietary nutrients were determined with AIA (acid insoluble ash) as a marker. After a 17 day treatment, three piglets were killed from each of the groups. Tissue samples of small and large intestine for light and electron microscopy examination were taken immediately after the opening of abdomen. Then, the weight or size of relevant organs was measured. The results show that the digestibilities of dry matter (DM), crude protein (CP) and fat were higher in Min piglets than in Landrace piglets (p<0.05). The diets containing processed soybeans had a significant higher CP digestibility than the control diet and the diets containing raw soybeans (p<0.05). Landrace piglets had heavier and longer small intestines, heavier kidneys and a lighter spleen than Min piglets (p<0.05). The pancreas of the animals fed the diets containing processed soybeans was heavier than that of the animals fed control diet (p<0.05) and the diets containing raw soybeans. But, the differences between raw and processed soybean diets were not significant. A significant interaction (p<0.05) between diet and pig breed was observed in weight of the small intestine. The Landrace piglets increased the weight in their small intestine when they were fed the diets containing soybeans. In the light micrographs and electron scanning micrographs, it was found that the villi of small intestinal epithelium of animals (especially Landrace piglets) fed the diets containing raw Chinese soybeans were seriously damaged. The transmission electron micrograph showed that a lot of vesicles were located between the small intestinal microvilli of these piglets. The histological examination also indicated that the proportion of goblet cells in villi and crypts in the piglets consuming the control diet was significantly lower (p<0.01 and p<0.02, respectively) than those of the animals consuming the diets containing raw or processed soybeans.

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References

  1. De Oliviera, J. T. A., A. Pusztai and G. Grant. 1988. Changes in organs and tissues induced by feeding purified kidney bean (Phaseolus vulgaris) lectin. Nutrition Research. 8:943-947. https://doi.org/10.1016/S0271-5317(88)80133-7
  2. Duan, Y. C. 1984. Morphological differences in gastrointestinal tract between Harbin White and Min pigs, Journal of Northeast Agricultural College. Suppl(1984):12-18.
  3. Fevrier, C., D. Bourdon and A. Aumaittre. 1988. Digestive capacity of the Chinese pig-effect of dietary fibre on digestibility and intestinal and pancreatic enzymes. Proc. 4th Sem. Digestive Physiology in the Pig, 6-10 June, 1988, Jablonna, Poland. pp. 172-179.
  4. Grant, G., S. W. B. Ewen, S. Bardocz, D. S. Brown, P. M. Dorward, W. B. Watt, J. C. Stewart and A. Pusztai. 1988. Local (gut) and systemic responses of rats to dietary soybean (Glycine Max) proteins. In: Recent Advances of Research in Antinutritional Factors in Legume Seeds (Ed. J. Huisman, A. F. B. van der Poel and I. E. Liener). Proceedings of the First International Workshop on 'Antinutritional Factors (ANFs) in Legume Seeds', November 23-25, 1988, Wageningen, The Netherlands. pp. 34-38.
  5. Grant, G., W. B. Watt, J. C. Stewart and A., Pusztai. 1987. Changes in the small intestine and hind-leg muscles of rats induced by dietary (Glycine max) protein. Medical Science Research. 15: 1355-1356.
  6. Han, W. Z., Z. D. Xiao, B. H. Cui and M. Q. Gao. 1983. A study on adversity-resistance of Min pigs. Chinese J. Anim. Sci. 30:15-17.
  7. Hendriks, H. G. C. J. M., M. J. L. Kik, J. F. J. G. Koninkx, T. S. G. A. M. Van den Ingh and J. M. V. M. Mouwen. 1991. Binding of kidney bean (Phaseolus vulgaris) isolectins to differentiated human colon carcinoma Caco-2 cells and their effects on cellular metabolism. In: Effects of Lectin in Legume Seeds on the Structure and Function of the Small Intestinal Mucosa (Ed. M. J. L. Kik).: In vivo and in vitro studies (Ph.D. Thesis, University of Utrecht). pp. 41-57.
  8. Herkelman, K. L. and G. L. Cromwell. 1990. Utilization of full-fat soybeans by swine reviewed. Feedstuffs 62(52):13-16,22.
  9. Herkelman, K. L., G. L. Cromwell, S. T. Stakly, T. W. Pfeiffer and D. A. Knabe. 1992. Apparent digestibility of amino acids in raw and heated conventional and low-trypsin-inhibitor soybeans for pigs. J. Anim. Sci. 70:818-826.
  10. Huisman, J., A. F. B. Van der Poel, Leeuwen, P. Van and M. W. A. Verstegen. 1990a. Com-parison of growth, nitrogen metabolism and organ weights in piglets and rats fed on the diets containing Phaseolus vulgaris beans. Br. J. Nutr. 64:743-753. https://doi.org/10.1079/BJN19900076
  11. Huisman, J., A. F. B. Van der Poel, J. M. V. M. Mouwen and E. J. Van Weerden. 1990b. Effect of variable protein contents in diets containing Phaseolus vulgaris beans on performance, organ weights and blood variables in piglets, rats and chickens. Br. J. Nutr. 64:755-764. https://doi.org/10.1079/BJN19900077
  12. Humason, G. H. 1967. PAS and feulgen techniques, and related reactions. In: Animal Tissue Techniques (Second edition) (Ed. G. H. Humason). W.H.Freeman and Company, San Francisco. pp. 303-309.
  13. Kakade, M. L., J. J. Bersch, J. E. Mcghee and G. Puskai. 1974. Determination of trypsin inhi-bitor activity of soy products: a collaborative analysis of an improved procedure. Cereal Chem. 51:376-382.
  14. Kik, M., J. Huisman, A. B. F. Van der Poel and J. Mouwen. 1988. Pathological changes of the small intestinal mucosa of piglets after feeding Phaseolus vulgaris beans. In: Recent Advances of Research in Antinutritional Factors in Legume Seeds (Ed. J. Huisman, A. F. B. van der Poel and I. E. Liener). Proceedings of First International Workshop on 'Antinutritional Factors (ANFs) in Legume Seeds', November 23-25, 1988, Wageningen, The Netherlands. pp. 49-53.
  15. Kik, M. J. L., J. F. J. G. Koninkx, A. J. C. Vanden Muysenberg and E. G. J. Hendriksen. 1991. Pathological effects of Phaseolus vulgaris isolectins on pig jejunal mucosa in organ culture. In: Effects of Lectin in Legume Seeds on the Structure and Function of the Small Intestinal Mucosa Maria (Ed. J. L. Kik).: In vivo and in vitro studies (Ph.D. Thesis, University of Utrecht). pp. 41-57.
  16. King, T. P., A. Pusztai and E. M. W. Clarke. 1982. Kidney bean (Phaseolus vulgaris) lectin-induced lesions in rat small intestine. 3. Ultrastructural studies. J. Comp. Pathol. 92:357-373. https://doi.org/10.1016/0021-9975(82)90021-4
  17. Li, D. X. and S. H. Wen. 1990. Scanning electron microscopy examination of gastric gland mucous membrane in healthy adult hens. Bulletin of Veterinary College of PLA. 10 (1):56-59.
  18. Liener, I. E. 1980 Protease inhibitors. Toxic constituents of plant foodstuffs. Academic Press. New York, US.
  19. Liener, I. E., Z. Nitsan, C. Srisangnam, J. J. Rackis and M. R. Gumbmann 1985. The USDA trypsin inhibitor study. II. Time related biochemical changes in the pancreas of rats. Qualitas Plantarum. Pl. Food Hum. Nutr. 35:243-257. https://doi.org/10.1007/BF01092197
  20. Marty, B. J. and E. R. Chaves. 1993. Effects of heat processing on digestible energy and other nutrient digestibilities of full-fat soybeans fed to weaner, grower and finisher pigs. Can. J. Anim. Sci. 13:411-419.
  21. Monari, S. 1993. Fullfat soybeans in pig feeding. Fullfat Soya Handbook, 2nd edn. American Soybean Association, Brussels, pp. 22-28.
  22. Nitsan, Z. and I. Nir. 1986. Accentuated response to soybean inhibitors by meal-feeding in various species. Adv. Exp. Med. Biol. 199:199-223.
  23. Noland, P. R., D. R. Campbell, R. K Gage, Jr, R. N. Sharp and Z. B. Johnson. 1976. Evaluation of processed soybeans and grains in diets for young pigs. J. Anim. Sci. 43(4):763-769.
  24. NRC, 1988. Nutrient Requirement for Domestic Animals. N0. 2. Requirements of Swine. Ninth Revised Ed.. National Academy of Science, National Research Council. Washington DC.
  25. Pusztai, A. 1988. Biological effects of dietary lectin. In: Recent Advances of Research in Antinutritional Factors in Legume Seeds (Ed. J. Huisman, A. F. B. van der Poel and I. E. Liener). Proceedings of the First International Workshop on 'Antinutritional Factors (ANFs) in Legume Seeds', November 23-25, 1988, Wageningen, The Netherlands. pp. 17-29.
  26. Qin, G. X. 1988. A study on corn distillers' dried grains as protein source for growing and finishing pigs. M.Sc. Thesis, Jilin Agricultural University.
  27. Qin, G. X., Verstegen, M. W. A. and M. W. Bosch. 1995. Variation of digestive capacity between genetically different pig populations: a review. J. Anim. Physiol. Anim. Nutr. 73:233-242. https://doi.org/10.1111/j.1439-0396.1995.tb00423.x
  28. Qin, G. X. and P. Chen. 1995. Antinutritional factors in soybeans, and feeding full-fat soybeans for pigs. Paper presented at the 4th Symposium of Northeast Pig Production Research council. Nov.20-22nd, 1995, Changchun, P.R. China.
  29. Qin, G. X., E. R. Ter Elst, M. W. Bosch and A. F. B. Van der Poel. 1996. Thermal processing of whole soya beans: Studies on the inactivation of antinutritional factors and effects on ileal digestibility in pigs. Anim. Feed Sci. Technol. 57: 313-324. https://doi.org/10.1016/0377-8401(95)00863-2
  30. Qin, G. X., A. F. B. Van der Poel and M. W. A. Verstegen. 1998. Effect of temperature and time during steam treatment on the protein quality of full-fat soybeans from different origins. J. Sci. Food Agric. 77:393-398 https://doi.org/10.1002/(SICI)1097-0010(199807)77:3<393::AID-JSFA59>3.0.CO;2-M
  31. SAS, 1989. $SAS/STAT^R$ User's Guide, Version 6, Fourth Edition, Vol.2, Cary, NC. SAS Institute Inc.
  32. Sibrits, F. K. 1981. The utilization of maize distillers dried grains in pig growth diets. South African J. Anim. Sci. 11(1):11-15.
  33. Vandergrift, W. L., D. A. Knabe, T. D. Tanksley, Jr. and S. A. Anderson. 1983. Digestibility of nutrients in raw and heated soyflakes for pigs. J. Anim. Sci. 57(5):1215-1224.
  34. Van der Poel, A. F. B., H. L. M. Aarts and M. J. L. Kik. 1990. Air classification of bean flour - effects on protein, antinutritional factors and effect of fines fraction on cultured explants of small intestine mucosa. J. Sci. Food Agric. 53:143-157. https://doi.org/10.1002/jsfa.2740530202
  35. Van der Poel, A. F. B., P. W. Mollee, J. Huisman and I. E. Liener. 1990a. Variation among species of animals in response to the feeding of heat-processed beans (Phaseolus vulgaris L.).1.Bean processing and effects on growth, digestibility and organ weight in piglets. Livest. Prod. Sci. 25:121-135. https://doi.org/10.1016/0301-6226(90)90046-9
  36. Van der Poel, A. F. B., I. E. Liener, P. W. Mollee and J. Huisman. 1990b. Variation among species of animals in response to the feeding of heat-processed beans (Phaseolus vulgaris L.).2.Growth and organ weights of chicken and rats and digestibility for rats. Livset. Prod. Sci. 25:137-150. https://doi.org/10.1016/0301-6226(90)90047-A
  37. Van der Poel, A. F. B., J. Blonk, D. J. Van Zuilichem and M. G. Van Oort. 1990c. Thermal inactivation of lectin and trypsin inhibitor activity during steam processing of dry beans (Phaseolus vulgaris L.) and effect on protein quality. J. Sci. Food Agric. 53:215-228. https://doi.org/10.1002/jsfa.2740530209
  38. Van Oort, M. G., R. J. Hamer and E. A. Slager. 1989. The trypsin inhibitor assay: improvement of an existing method. In: Recent Advances of Research in Antinutritional Factors in Legume Seeds. (Ed. J. huisman, A. F. B. van der Poel, I. E. Liener). PUDOC, Wageningen, The Netherlands, pp. 110-113.
  39. isser, A. and G. H. Tolman. 1993. The influence of various processing condition on the level of antinutritional factors in soya protein products and their nutritional value for calves. In:Recent Advances of Research in Antinutritional Factors in Legume Seeds. (Ed. A. F. B. van der Poel, J. Huisman and H. S. Saini). Proceedings of 2nd International Workshop on Antinutritional factors (ANFs) in Legume Seeds. Wageningen, The Netherlands, 1-3 december 1993, pp. 447-453.
  40. Xian, J. and D. J. Farrell. 1991. The nutritive value of microwaveprocessed raw soya beans determined with chickens, rats and rabbits. Anim. Feed Sci. Technol. 34:127-139. https://doi.org/10.1016/0377-8401(94)90196-1
  41. Yang, S. X. 1983. Nutritional Evaluating Methods for Feedstuffs. People's Publish of Gansu Province, P.R.China.
  42. Yen, J. T., T. Hymowitz and A. H. Jensen. 1974. Effects of soybeans of different trypsin-inhibitor activities on performance of growing swine. J. Anim. Sci. 38(2):304-309.
  43. Zhao, G. 1989. In: Research on Min Pigs. Heilongjiang Sci. Technol. Press. pp. 145-185.

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