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Fatty Acid and Volatile Oil Compositions of Allomyrina dichotoma Larvae

  • Youn, Kumju;Kim, Ji-Young;Yeo, Hyelim;Yun, Eun-Young;Hwang, Jae-Sam;Jun, Mira
    • Preventive Nutrition and Food Science
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    • v.17 no.4
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    • pp.310-314
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    • 2012
  • Thirty-two different volatile oils were identified from Allomyrina dichotoma (A. dichotoma) larvae by gas chromatography/mass spectrometry (GC/MS). The major volatile components were 2,2,4-trimethyl-3-carboxyisopropyl pentanoic acid isobutyl ester (5.83%), phenol,2,6-bis(a,a-dimethyl ethyl)-4-(1-methyl-1-phenylethyl) (5.72%), heptacosane (5.49%) and phenol,2,4-bis(1-methyl-1-phenylethyl) (5.47%). The composition of the fatty acids in A. dichotoma larvae was also determined by gas chromatography (GC) and fourteen constituents were identified. Oleic acid (19.13%) was the most abundant fatty acid followed by palmitic acid (12.52%), palmitoleic acid (3.71%) and linoleic acid (2.08%) in 100 g of A. dichotoma larvae on a dry weight basis. The quantity of unsaturated fatty acids (64.00%) were higher than that of saturated ones (36.00%). The predominant fatty acids in A. dichotoma consist of monounsaturated fatty acid (MUFA, 57.70%) such as oleic acid, myristoleic acid and palmitoleic acid, followed by saturated fatty acids (36.00%) and polyunsaturated fatty acids (PUFA, 6.50%). In particular, the presence of essential fatty acids, such as linoleic (5.30%) and linolenic acid (0.40%) give A. dichotoma larvae considerable nutritional and functional value and it may be a useful source for food and/or industrial utilization.

Comparison of Condenser Characteristics using R134a and R22 under the Same Inlet Temperature Condition (동일한 유입온도조건에서 R134a와 R22 적용 응축기의 특성비교)

  • Kang, Shin-Hyung;Byun, Ju-Suk;Kim, Chang-Duk
    • Journal of Energy Engineering
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    • v.15 no.3 s.47
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    • pp.166-173
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    • 2006
  • R134a is considered as an alternative refrigerant to R22 for air conditioners. An experimental investigation was made to study the characteristics of the heat transfer and pressure drop for R134a flowing in a fin-and-tube heat exchanger used for commercial air-conditioning units. Experiments were carried out under the conditions of inlet refrigerant temperature of $60^{\circ}C$ and refrigerant mass fluxes of $150,\;200,\;and\;250\;kg/m^{2}s$. The inlet air has dry bulb temperature or $35^{\circ}C$, relative humidity of 40% and air velocity varying from 0.68 to 1.6 m/s. Experiments show that air velocity decreased by 5.9% is needed for R134a than that of R22 while pressure drop for R134a was $18.1{\sim}20.4%$ higher than that of R22 for the degree of subcooling $5^{\circ}C$. The results are useful in designing more compact and effective condensers for various refrigeration and air conditioning systems using refrigerant R134a.

A Study on Freezing Assessment of the Water in the Z-Trap of Sewage Bay (PVC 오수받이 내부 Z형 트랩의 동결성 평가에 관한 연구)

  • Lee, Changwoo;Oh, Seungju
    • Journal of the Society of Disaster Information
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    • v.12 no.1
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    • pp.98-104
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    • 2016
  • This study clarifies the simulation on freezing of remaining water in the Z-trap set up in the PVC sewage bay to control malodor. It made use of commercial FEM LAB program(ver. 3.2) well known as a solution of the problems arising in the flow of various fluid, heat transfer and mass transfer. Simulation results under the temperature $-20^{\circ}C$ outwards show that the water in the Z-trap set up in the sewage bay to control malodor freeze in the 60cm under the ground level after 14 days in the wet ground, and after 17 days in the regular ground. On the other hand, if the soil is dry even after the 42 days does not go down below freezing. Therefore, the water in the Z-trap was confirmed that it does not freeze.

Identification and Quantitative Determination of Glucosinolates in Brassica napus cv. Hanakkori

  • Kim, Sun-Ju;Fujii, Kouei;Mohamed, Zaidul Islam Sarker;Kim, Hyun-Woong;Yamauchi, Hiroaki;Ishii, Gensho
    • Food Science and Biotechnology
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    • v.17 no.5
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    • pp.1097-1101
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    • 2008
  • The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. 'Hanakkori' materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In 'Hanakkori', 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL).

Efficacy of Supplemental Microbial Phytase on Laying Performance and Phosphorus Utilization I. Effect of Microbial Phytase at Different Phosphorus Levels on Laying Performance and Phosphorus Utilization (산란 생산성과 인 이용성에 대한 Microbial Phytase의 첨가 효과 I. 무기태인 수준이 다른 사료에 Microbial Phytase 첨가가 산란상 및 인 이용성에 미치는 영향)

  • 김상호;유동조;나재천;최철환;상병돈;이상진;이원준;류경선
    • Korean Journal of Poultry Science
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    • v.27 no.1
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    • pp.19-23
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    • 2000
  • The effects of microbial phutase on laying performance and phosphorus utilization were examined at different levels of dietary nonphosphorus(NPP) in 320 23-week-old Hy-line brown hens for 12weeks. Diets were formulated 0.275%(T1), 0.220%(T2), 0.165%(T3) of NPP levels, and supplemental microbial phytase was 300DPU/kg diet constantly. Conventional diet(C) was formulated 0.275% NPP level without microbial phytase. Egg production and egg mass were higher in T2 than the others (p<0.05), and average egg weight was higher in T1 than the other (p<0.05). Egg productivity was tended to increase with supplemental phytase compared to conventional diet. Daily feed intake a hen also increased in T2 (p<0.05). Feed conversion ratio was improved slightly without significant difference. Eggshell breaking strength and thickness were not different significantly among the treatments. Haugh unit and yolk color were also not different. Calcium and phosphorus retention in body increased in T2 (p<0.05), but dry matter and nitrogen retention were not different significantly. Differences in nitrogen and calcium excretions were not found among the treatments. But phosphorus excretion decreased in order of dietary phosphorus levels with supplement phytase compared to C (p<0.05). Tibial ash, calcium and phosphorus were similar among the treatments. In conclusion, supplemental microbial phytase in laying diet may help to utilize phytase phosphorus, and could decrease NPP intake.

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High-level Production of Recombinant Human IFN-$\alpha2a$ with Co-expression of $tRNA^{Arg(AFF/AGA)}$ in High-cell-density Cultures of Escherichia coli

  • Shin, Chul-Soo;Hong, Min-Seon;Shin, Hang-Chel;Lee, Jeewon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.4
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    • pp.301-305
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    • 2001
  • The co-expression of the arg U gene in a double-vector expression system of recombi-nant Escherichia coli BL22(DE3)[pET-IEN2a+pAC-argU] significantly enhanced the production level of reconminant human interferon -$\alpha$2a(rhIFN-$\alpha$2a) in high cell density cultures, compared to a recombinant E. coli culture containing only the single expression vector, pET-IEN2a. The dry cell mass concentration increased to almost 100 g/L, and more than 4 g/L of rhIFN-$\alpha$2a was accumu-lated in the culture broth. Evidently, the synthesis of rhIFN-$\alpha$2a was strongly dependent on the pre-induction growtih rate and more efficient at a higher specific growth rate. The additional sup-ply of tRN $A^{Arg(AGG/AGA)}$ enhanced the expression level of the rhIFN-$\alpha$2a gene in the early stage of the post-induction phase, yet thereafter the specific production rate of rhIFN-$\alpha$2a rapidly de-creased due to severe segregational instability of plasmid vector pET-IEN2a. It would appear that the plasmid instability with only occurred to pET-IEN2a in the double vector system, was re-lated to the effect of translational stress due to the over expression of rhIFN-$\alpha$2a.

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Comparison of Heat Transfer Performance and Pressure Drop of Fin-Tube and Aluminum Heat Exchangers (핀-튜브 열교환기와 알루미늄 열교환기의 전열성능과 압력강하 특성비교)

  • Chang, Keun-Sun;Lee, Hyun-Su;Kim, Jae-Duck
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.2
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    • pp.222-229
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    • 2009
  • This study presents comparison of heat transfer and air side friction characteristics in a condenser condition of air conditioner between Louver fin-tube heat exchangers and aluminum parallel heat exchangers. All experiments are performed using an air-enthalpy type calorimeter, which is designed based on the method described in ASHRAE standards. The air velocities crossing the heat exchanger tubes are varied from 0.7 to 1.6 m/s with 0.3 m/s interval, maintaining air dry temperature and relative humidity at $20^{\circ}C$ and 60% respectively. Water temperature and flow rate inside the tube are $70^{\circ}C$ and 10 LPM, respectively. Experimental results show that the heat transfer performances of aluminum heat exchangers are 17-163% higher than those of Louver fin-tube heat exchangers based on the data per unit volume, mass, and heat transfer area, whereas air side pressure drops of aluminum heat exchangers are 19-81% lower.

Anti-oxidative and Anti-hyperglycemia Effects of Triticum aestivum Wheat Sprout Water Extracts on the Streptozotocin-induced Diabetic Mice (밀순 물추출물의 항산화 효과 및 Streptozotocin으로 유발한 당뇨 흰쥐에서 혈당강하에 미치는 영향)

  • Lee, Sun-Hee;Lee, Young-Mi;Lee, Hoi-Seon;Kim, Dae-Ki
    • Korean Journal of Pharmacognosy
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    • v.40 no.4
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    • pp.408-414
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    • 2009
  • This study was performed to investigate the anti-hyperglycemia effects of the Triticum aestivum wheat sprout (TAWS) water extracts in the diabetic mice. Diabetic experimental model was established by intraperitoneal injection of streptozotocin into male Balb/c mice. Mice were divided into five groups: normal (CON), diabetic control (DM), and three experimental groups (DM-100, diabetes with TAWS extracts 100mg/kg; DM-50, diabetes with TAWS extracts 50 mg/kg; DM-25, diabetes with TAWS extracts 25 mg/kg). TAWS extracts were administered orally in diabetic mice. Body weight, food intake, and blood glucose levels were recorded for 12 days and blood insulin levels were measured at the day 12. Oral administration of TAWS extracts reduced slightly food intake and induced a little body weight gain in DM-100 groups. The blood level of glucose was decreased in the dose-dependent manner; 55% in the DM-100 group and 39.7% in the DM-50 group. The blood level of insulin also was improved 10 folds in the DM-100 group and 3.6 folds in the DM-50 group compared to the DM group. The contents of total phenolic compounds and total flavonoids in 1 g dry mass of TAWS extracts were 6.6 mg of tannic acid equivalents and 1.0 mg of 8-hydroquinolline equivalents, respectively. In addition, the antioxidant and DPPH radical scavenging activity of TAWS extracts were 1.2 mM and 1.8 mM ascorbic acid equivalents, respectively. These results suggest that TAWS water extracts could contribute to attenuate clinical symptoms of diabetes mellitus.

Antagonistic Activity against Dirty Panicle Rice Fungal Pathogens and Plant Growth-Promoting Activity of Bacillus amyloliquefaciens BAS23

  • Saechow, Sukanya;Thammasittirong, Anon;Kittakoop, Prasat;Prachya, Surasak;Thammasittirong, Sutticha Na-Ranong
    • Journal of Microbiology and Biotechnology
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    • v.28 no.9
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    • pp.1527-1535
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    • 2018
  • Bacterial strain BAS23 was isolated from rice field soil and identified as Bacillus amyloliquefaciens. Based on dual culture method results, the bacterium BAS23 exhibited potent in vitro inhibitory activity on mycelial growth against a broad range of dirty panicle fungal pathogens of rice (Curvularia lunata, Fusarium semitectum and Helminthosporium oryzae). Cell-free culture of BAS23 displayed a significant effect on germ tube elongation and mycelial growth. The highest dry weight reduction (%) values of C. lunata, H. oryzae and F. semitectum were 92.7%, 75.7%, and 68.9%, respectively. Analysis of electrospray ionization-mass spectrometry (ESI-MS) and $^1H$ nuclear magnetic resonance (NMR) spectroscopy revealed that the lipopeptides were iturin A with a C14 side chain (C14 iturinic acid), and a C15 side chain (C15 iturinic acid), which were produced by BAS23 when it was cultured in nutrient broth (NB) for 72 h at $30^{\circ}C$. BAS23, the efficient antagonistic bacterium, also possessed in vitro multiple traits for plant growth promotion and improved rice seedling growth. The results indicated that BAS23 represents a useful option either for biocontrol or as a plant growth-promoting agent.

Meat Quality of Lambs Fed on Palm Kernel Meal, a By-product of Biodiesel Production

  • Ribeiro, R.D.X.;Oliveira, Ronaldo Lopes;Macome, F.M.;Bagaldo, A.R.;Silva, M.C.A.;Ribeiro, C.V.D.M.;Carvalho, G.G.P.;Lanna, D.P.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.10
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    • pp.1399-1406
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    • 2011
  • This study aimed to establish the optimum level of palm kernel meal in the diet of Santa Ines lambs based on the sensorial characteristics and fatty acid profile of the meat. We used 32 lambs with a starting age of 4 to 6 months and mean weight of $22{\pm}2.75kg$, kept in individual stalls. The animals were fed with Tifton-85 hay and a concentrate mixed with 0.0, 6.5, 13.0 or 19.5% of palm kernel meal based on the dry mass of the complete diet. These levels formed the treatments. Confinement lasted 80 days and on the last day the animals were fasted and slaughtered. After slaughter, carcasses were weighed and sectioned longitudinally, along the median line, into two antimeres. Half-carcasses were then sliced between the 12th and 13th ribs to collect the loin (longissimus dorsi), which was used to determine the sensorial characteristics and fatty acid profile of the meat. For sensorial evaluation, samples of meat were given to 54 judges who evaluated the tenderness, juiciness, appearance, aroma and flavor of the meat using a hedonic scale. Fatty acids were determined by gas chromatography. The addition of palm kernel meal to the diet had no effect on the sensorial characteristics of meat juiciness, appearance, aroma or flavor. However, tenderness showed a quadratic relationship with the addition of the meal to the diet. The concentration of fatty acids C12:0, C14:0 and C16:0 increased with the addition of palm kernel meal, as did the sum of medium-chain fatty acids and the atherogenicity index. Up to of 19.5% of the diet of Santa Ines lambs can be made up of palm kernel meal without causing significant changes in sensorial characteristics. However, the fatty acid profile of the meat was altered.