• Title/Summary/Keyword: dry mass

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A New Raw-Starch-Digesting ${\alpha}$-Amylase: Production Under Solid-State Fermentation on Crude Millet and Biochemical Characterization

  • Maktouf, Sameh;Kamoun, Amel;Moulis, Claire;Remaud-Simeon, Magali;Ghribi, Dhouha;Chaabouni, Semia Ellouz
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.489-498
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    • 2013
  • A new Bacillus strain degrading starch, named Bacillus sp. UEB-S, was isolated from a southern Tunisian area. Amylase production using solid-state fermentation on millet, an inexpensive and available agro-resource, was investigated. Response surface methodology was applied to establish the relationship between enzyme production and four variables: inoculum size, moisture-to-millet ratio, temperature, and fermentation duration. The maximum enzyme activity recovered was 680 U/g of dry substrate when using $1.38{\times}10^9$ CFU/g as inoculation level, 5.6:1 (ml/g) as moisture ratio (86%), for 4 days of cultivation at $37^{\circ}C$, which was in perfect agreement with the predicted model value. Amylase was purified by Q-Sepharose anion-exchange and Sephacryl S-200 gel filtration chromatography with a 14-fold increase in specific activity. Its molecular mass was estimated at 130 kDa. The enzyme showed maximal activity at pH 5 and $70^{\circ}C$, and efficiently hydrolyzed starch to yield glucose and maltose as end products. The enzyme proved its efficiency for digesting raw cereal below gelatinization temperature and, hence, its potentiality to be used in industrial processes.

Comparison of the Nutritional Value of Chlorella ellipsoidea and Nannochloris oculata for Rotifers and Artemia Nauplii

  • Cabrera Tomas;Bae Jean Hee;Bai Sungchul C.;Hur Sung Bum
    • Fisheries and Aquatic Sciences
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    • v.8 no.4
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    • pp.201-206
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    • 2005
  • Microalgae are widely used for mass culture of the rotifer Brachionus plicatilis in aquaculture. Since the nutritional value of the rotifer is closely related to its food, the nutritional value of its food should be known in detail. Chlorella ellipsoidea and Nannochloris oculata are re­presentative food organisms for rotifers that are easily cultured. Therefore, the nutritional values of these micro algae were examined for ultrasmall, small, and large rotifers and Artemia nauplii. Chlorella ellipsoidea contained seven times more total fatty acids than N. oculata. The three types of rotifer fed N. oculata contained more amino acids than those fed C. ellipsoidea. However, the total fatty acids of the rotifers fed each microalga species differed according to the type of rotifer. Newly hatched Artemia nauplii contained more protein and had a higher dry weight than those fed microalgae for 6 h. As with the rotifers, the Artemia nauplii fed N. oculata contained more protein and amino acids than those fed C. ellipsoidea, while the reverse was true for the total fatty acid content. Our results suggest that N. oculata is a good supply of protein, while C. ellipsoidea is a good source of lipids as food organisms for rotifers and Artemia nauplii in aquaculture.

Engineering behavior of expansive soils treated with rice husk ash

  • Aziz, Mubashir;Saleem, Masood;Irfan, Muhammad
    • Geomechanics and Engineering
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    • v.8 no.2
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    • pp.173-186
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    • 2015
  • The rapid urbanization in Pakistan is creating a shortage of sustainable construction sites with good soil conditions. Attempts have been made to use rice husk ash (RHA) in concrete industry of Pakistan, however, limited literature is available on its potential to improve local soils. This paper presents an experimental study on engineering properties of low and high plastic cohesive soils blended with 0-20% RHA by dry weight of soil. The decrease in plasticity index and shrinkage ratio indicates a reduction in swell potential of RHA treated cohesive soils which is beneficial for problems related to placing pavements and footings on such soils. It is also observed that the increased formation of pozzolanic products within the pore spaces of soil from physicochemical changes transforms RHA treated soils to a compact mass which decreases both total settlement and rate of settlement. A notable increase in friction angle with increase in RHA up to 16% was also observed in direct shear tests. It is concluded that RHA treatment is a cost-effective and sustainable alternate to deal with problematic local cohesive soils in agro-based developing countries like Pakistan.

Studies on Production of High-Quality Cornus controversa Container Seedlings (층층나무 우량 용기묘 생산에 관한 연구)

  • 윤택승;홍성각
    • Journal of Korea Foresty Energy
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    • v.21 no.3
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    • pp.28-33
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    • 2002
  • This study was carried out to develop the technique for the production of high quality container seedlings of Comus controversa Hemsley. The seedlings were grown on the medium of peatmoss : perlite : vermiculite (1:1:1, v/v) in plastic net container and no-net plastic container as control for five months in the PE house. The seedlings grown in the plastic net container showed better root collar diameter growth, better development of long and fine roots, more increment of dry mass of roots and shoots than those grown in the no-net container. But the height growth of the seedlings in both container was similar. In particular the seedlings grown in plastic net container had no spiraling roots which were always observed in the control container seedlings. This result was induced by air-root pruning effect from the plastic net container.

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Production of Chiral (S)-styrene Oxide by Rhodosporidium sp. SJ-4 which has an Epoxide Hydrolase Activity (에폭사이드 가수분해효소를 갖는 Rhodosporidium sp. SJ-4를 이용한 광학활성 (S)-styrene Oxide의 생산)

  • Yoo, Seung-Shick;Lee, Eun-Yeol;Kim, Hee-Sook;Kim, Jung-Sun;Oh, You-Kwan;Park, Sung-Hoon
    • Journal of Institute of Control, Robotics and Systems
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    • v.11 no.10
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    • pp.857-863
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    • 2005
  • A yeast strain utilizing styrene epoxide as a sole carbon and energy source was isolated from soil samples for the production of enantiopure of styrene epoxide by kinetic resolution. The strain, identified as a Rhodosporidium toruloides SJ-4, expressed an epoxide hydrolase which preferentially converted (R)-styrene epoxide into the corresponding diol. A maximum activity of 135 U/L was observed when biomass (dry cell mass) reached 6.7 g/L at 21 h of batch culture. Under the partially optimized reaction conditions ($35^{\circ}C$ and pH 8.0), the optically pure (S)-styrene epoxide was obtained with the yield of 21% when the initial substrate concentration was 100 mM. The reaction was completed at 9 h.

Comparison of Condenser Characteristics Using R407C and R22 on the Same Inlet Temperature Condition (동일한 유입온도조건에서 R407C와 R22 적용 응축기의 특성비교)

  • 김창덕;전창덕;이진호
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.15 no.7
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    • pp.595-603
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    • 2003
  • R407C is considered as an alternative refrigerant to R22 for air conditioners. An experimental investigation was made to study the characteristics of the condensation heat transfer and pressure drop for R407C flowing in a fin-and-tube heat exchanger used for commercial air-conditioning units. Experiments were carried out under the conditions of inlet refrigerant temperature of 6$0^{\circ}C$ and refrigerant mass flux varying from 150 to 250 kg/$m^2$s for refrigerant side. The inlet air has dry bulb temperature of 35$^{\circ}C$ , relative humidity of 50% and air velocity varying from 0.8 to 1.6 m/s. Experiments show that air velocity increased by 25% is needed for R407C than that of R22 for subcooling temperature of 5$^{\circ}C$, which resulted in air-side pressure drop increase of 28.8% for R407C as compared to R22. As a consequence, in order to provide the same design condition of a condenser, the fan requires higher electric-power consumption with R407C than that with R22.

Effects of Hydrophilic Surface Treatment on Condensation Heat Transfer at the Outside Wall of Horizontal Tube (수평관 외벽에서 친수성 표면처리가 응축열전달에 미치는 영향)

  • 황규대;박노성;강병하
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.12 no.6
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    • pp.533-540
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    • 2000
  • Condensation heat transfer characteristics have been investigated experimentally when a water vapor is condensed on the outside of a horizontal copper tube in a condenser. This problem is of particular interest in the design of a LiBr-water absorption system. Hydrophilic surface modification was performed to increase the wettability on the copper tube. The optimum hydrophilic treatment condition using acethylene and nitrogen as reaction gas is also studied in detail. The results obtained indicate that the optimum reaction gas ratio of acethylene to nitrogen for hydrophilic surface modification was found to be 7 : 3 for the best condensation heat transfer. In the wide ranges of coolant inlet temperatures, and coolant mass flow rates, both the condensation heat transfer rate and the condensation heat transfer coefficient of a hydrophilic copper tube are increased substantially, compared with those of a conventional copper tube used in a condenser. It is also found that the condensation heat transfer enhancement by the hydrophilic surface modification still emains even after a hundred cycles of wet/dry processes.

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Citric Acid Production and Scale-up in Dual Hollow Fiber Bioreactor (이중실관 생물 반응기에서의 구연산 생산과 Scale-up)

  • 장호남;지동진;심상준
    • Membrane Journal
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    • v.2 no.2
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    • pp.122-128
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    • 1992
  • A study on the citric acid production was performed in various size dual hollow fiber bioreactors with immobilized Aspergillus niger (KCTC 1232). The final dry cell mass density reached 300g/l based on the space volume available for cell growth. Under air and oxygen aeration the volumethe productivity reached 0.63 and 1.02g/l.h, which cormsponded to 10 and 16 fold over those of batch fermentation, respectively. The initial pH of the medium was a critical factor and the lower value resulted in higher citric acid yield. The increase in the feeding rate of medium or the number of reactor unit resulted in the improvement of the productivity due to higher consumption rate of substrate.

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Optimum Mix Design for Waste Newsprint Paper Fiber Reinforced Cement Composites (폐지섬유보강 시멘트 복합체의 최적배합비 도출)

  • 원종필;배동인;박찬기;박종영
    • Journal of the Korea Concrete Institute
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    • v.13 no.4
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    • pp.346-353
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    • 2001
  • This research investigates the mixture proportioning of waste newsprint paper fiber for thin-cement product. Waste newsprint paper fibers obtained through shredded mechanically by a dry process. Waste newsprint paper fiber reinforced cement composites was manufacted by slurry-dewatering method. The waste newsprint paper fiber reinforcement conditions (fiber mass fraction, level of substitution of virgin fibers, level of fiber beating) and processing variables (pressed, unpressed) are optimized through experimental studies and statistical analyses based on factorial design of experiments and analyses of variance. The optimized recycled waste newsprint paper fiber reinforced cement composites were technically evaluated. The results are shown to possess acceptable properties and strong potentials of the recycling of waste newsprint paper of the reinforcement of thin-cement products.

Performance Comparison between Indirect Evaporative Cooler and Regenerative Evaporative Cooler made of Plastic/Paper (플라스틱/종이 재질의 간접 증발 소자와 재생 증발 소자 성능 비교)

  • Kim, Nae-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.1
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    • pp.88-98
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    • 2016
  • The Korean summer is hot and humid, and air-conditioners consume considerable amounts of electricity. In such cases, the simultaneous use of indirect evaporative coolers may help reduce the sensible heat and save electricity. In this study, heat transfer and pressure drop characteristics of indirect or regenerative evaporative coolers made from plastic/paper are investigated. The results showed that heat and mass transfer model based on the ${\epsilon}-NTU$ method predicted the indirect evaporation efficiencies, cooling capacities and pressure drops adequately. Both for indirect or regenerative evaporative cooler, the indirect evaporation efficiency increased with increasing dry channel inlet temperature or relative humidity. The indirect evaporation efficiency of the regenerative evaporative cooler was larger than that of the indirect evaporative cooler.