• Title/Summary/Keyword: dry mass

Search Result 691, Processing Time 0.025 seconds

Purification and Glycosylation Pattern of Human L-Ferritin in Pichia pastoris

  • Lee, Jong-Lim;Yang, Seung-Nam;Park, Cheon-Seok;Jeoung, Doo-Il;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
    • /
    • v.14 no.1
    • /
    • pp.68-73
    • /
    • 2004
  • Ferritin is an iron storage protein found in most living organisms. For expression and industrial use, human light chain ferritin (L-ferritin) was cloned from human liver cDNA library and expressed in Pichia pastoris strain GS115. The recombinant L-ferritin in Pichia pastoris was glycosylated. In a fed-batch culture, the cell mass reached about 57 g/l of dry cell weight, and the L-ferritin in the cell was increased to about 95 mg/l after 150 h. In an atomic absorption spectrometry analysis, the intracellular content of iron in the L-ferritin transformant was measured as $1,694{\pm}85\;\mu\textrm{g}g/g$, which is 5.4-fold more than that of the control strain. This L-ferritin transformant could serve as iron-fortified nutrients in animal feed stock.

New Approach to Pressure Control of a Impression Cylinder for Roll Coater (인쇄성능 향상을 위한 롤코터용 임프레션 실린더의 압력 제어)

  • Yun, S.N.;Ham, Y.B.;Park, J.H.
    • Journal of Power System Engineering
    • /
    • v.13 no.3
    • /
    • pp.59-64
    • /
    • 2009
  • This study presents a new approach to pressure control of a impression cylinder for roll coater which is a kind of face pressure control between blanket roll and impression roll. Roll-to-Roll method for printing is a very useful tool for mass production such as RFID elements, smart sensors and solar cell devices. In this study, a decupling control strategy of the roll coater which is a combination of a cylinder system, a dry system and two pressure regulators with two pneumatic cylinders was discussed. Also, the characteristics of component such as a pressure regulator having a pressure reducing function and the movement of a blanket roll and a impression cylinder were analyzed using the Matlab software. From this results, the techniques of a shock and a vibration reduction were suggested.

  • PDF

An Experimental Study on Condensation Characteristics at Various Condensation Pressure of R407C (응축압력 변화에 따른 R407C의 응축특성에 관한 연구)

  • 전창덕;장경근;김창덕;이진호
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
    • /
    • v.15 no.3
    • /
    • pp.230-238
    • /
    • 2003
  • R407C is considered as alternative refrigerant of R22 for air conditioners. Experimental investigation is made to study the condensation heat transfer characteristics of slit fin-tube heat exchanger using alternative refrigerant, R407C. Experiments are carried out at condensation pressure of 2110 kPa and 1943 kPa with the degree of superheat of 1$0^{\circ}C$ and mass flux varying from 150 to 250 kg/$m^2$s for refrigerant side. The inlet air condition is dry bulb temperature of 35$^{\circ}C$, relative humidity of 50% and air velocity varying from 0.8 to 1.6 m/s. Experiments show that pressure drop gets smaller at a higher condensation pressure especially when condensation pressure is raised from 1943 to 2110 kPa. Heat transfer rate gets smaller at a lower condensation pressure in the range of experimental condition.

Comparative Study of Fluidized Bed-type and Assmann Psychrometer (유동층습도계와 아스만습도계의 비교 연구)

  • 강희찬;강민철
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
    • /
    • v.15 no.9
    • /
    • pp.779-785
    • /
    • 2003
  • The present work proposed a newly developed fluidized bed-type psychrometer that could be applicable to the high temperature and contaminant gases. The psychrometer is a device that test gas passes through the water in a glass bottle as a bubbly flow to obtain the adiabatic saturation temperature. A fluidized bed-type psychrometer was made and its characteristics were compared with the Assmann Psychrometer. The characteristic time was in the order of a hundred seconds, and decreased for the decrease of the mass of water and the increase of the air flow rate. The air flow rate and the insulation of the glass bottle were definitely important in the accurate measurement of the wet bulb temperature. The error in wet bulb temperature became less than 5 percent of the difference of dry and wet bulb temperatures if the air velocity in the glass bottle was greater than 1.5m/s.

Etching Mechanism of Indium Tin Oxide Thin Films using Cl2/HBr Inductively Coupled Plasma

  • Kim, Sung-Ihl;Kwon, Kwang-Ho
    • Transactions on Electrical and Electronic Materials
    • /
    • v.10 no.1
    • /
    • pp.1-4
    • /
    • 2009
  • Dry etching characteristics of indium tin oxide films and etch selectivities over photoresist films were investigated using $Cl_2/HBr$ inductively coupled plasma. From a Langmuir probe diagnostic system, it was observed that while the plasma temperature was kept nearly constant in spite of the change of the HBr mixing ratio, the positive ion density decreases rapidly with increasing the mixing ratio. On the other hand, a quadrupole mass spectrometer showed that the neutral HBr and Br species increased. The etching mechanism in the $HBr/Cl_2$ plasma was analyzed.

A Study on the Performance Prediction of Paper Heat Exchanger for Exhaust Heat Recovery (배기열 회수용 종이 열교환기의 성능예측에 관한 연구)

  • Yoo, Seong-Yeon;Kim, Jin-Hyuck
    • Proceedings of the SAREK Conference
    • /
    • 2005.11a
    • /
    • pp.294-299
    • /
    • 2005
  • In order to control indoor air quality and save energy. it is needed to install a suitable ventilation system equipped with heat exchanger for heat recovery. Paper heat exchanger can recover $50{\sim}70$ of the enthalpy difference between supply and exhaust air. The purpose of this research is to obtain the experimental correlations for the friction factor, heat transfer coefficient, mass transfer coefficient and permeance of paper heat exchanger, which can be used for the performance prediction of the paper heat exchanger. Pressure drop at various velocities and heat transfer rate at various dry-bulb temperatures, relative humidities, and specific humidities are measured to make experimental correlations. The results of prediction using correlations show fairly good agreement with experimental data.

  • PDF

Lipid and Fatty Acid Composition of the Antarctic Krill Euphausia superba

  • Cho, Ki-Woong;Shin, Jong-Heon;Jung, Kyoung-Hwa
    • Ocean and Polar Research
    • /
    • v.21 no.2
    • /
    • pp.109-116
    • /
    • 1999
  • Total lipid content, lipid class and fatty acid composition of the Antarctic krill Euphausia superba collected from the water of King George Island, Antarctica during austral summer of 1997-1998, were investigated. The overall lipid content of 5. superba was 72 mg/g dry mass similar to the reported values for most temperate species. The neutral lipid of E. superba was 29% of the total lipid and that of phospholipid was 71%. The majority of neutral lipid was triacylglycerols(31.6% of neutral lipids) while phosphatidyl choline (44% of phospholipid) was the most abundunt in phospholipids. The quantitative composition of the fatty acid in E. superba show consisting mostly of the saturated 16:0 (16.6% -22.1%), along with the polyunsaturated 20:5(n-3) (20.6% -22.1%) and 22:6(n-3) (14.9% -16.9%) acids.

  • PDF

Optimization of SOD Biosynthesis by Controlling Sucrose Concentration in the Culture of Carrot Hairy Root

  • Kim, Ji-Hyeon;Yoo, Young-Je
    • Journal of Microbiology and Biotechnology
    • /
    • v.12 no.4
    • /
    • pp.617-621
    • /
    • 2002
  • In order to optimize the carrot hairy root culture for SOD production, a fed-batch culture of hairy roots was performed in a bioreactor. Maximum SOD activity was obtained when the hairy roots were transferred to the MS medium containing 110 g/1 concentration of sucrose. By controlling the sucrose concentration (70 g/1 sucrose for growth and 110 g/1 sucrose far production, respectively) In a two-stage fed-batch culture, 29 g/1 of the hairy roots was obtained based on the final dry mass. The volumetrically determined SOD activity and productivity in the fed-batch culture were about 6 times higher than those from the flask culture containing sucrose at 30 g/1 concentration.

Eco-friendly Solventless Synthesis of 5-Indolylpyrimido[4,5-d]pyrimidinones and Their Antimicrobial Activity

  • Gupta, Ragini;Jain, Anshu;Joshi, Rahul;Jain, Meenakshi
    • Bulletin of the Korean Chemical Society
    • /
    • v.32 no.3
    • /
    • pp.899-904
    • /
    • 2011
  • A series of 5-indolylpyrimido[4,5-d]pyrimidinones (4a-h) were obtained by multi-component reaction of 3-formylindole (1), thiobarbituric acid/barbituric acid (2) and thiourea/urea (3) under microwave irradiation in dry media. Secondly, grinding together neat reactants also gave the titled compounds in good yields. All the synthesized compounds have been characterized on the basis of elemental analyses and spectral data (IR, $^1H$ NMR and Mass). Representative compounds were also evaluated for their antimicrobial activity against Rhizopus stolonifer, Fusarium oxysporum, Escherichia coli, and Pseudomonas aeruginosa at different concentrations. Some of the compounds showed promising activity.

Continuous Xanthan Fermentations in a Three-Phase Fluidized Bed Bioreactor (삼상유동층 생물반응기에서의 연속식 Xanthan 발효)

  • 서일순;노희찬;허충회
    • KSBB Journal
    • /
    • v.17 no.1
    • /
    • pp.26-32
    • /
    • 2002
  • The aerobic bacterium Xanthomonas campestris was cultivated continuously in a three-phase fluidized bed bioreactor to produce extracellular polysaccharide xanthan, Fluidized particles of 8.0 mm glass beads were used for disintegrating the large air bubbles even at high viscosities to improve the gas-liquid oxygen transfer rate. Xanthin productivity [kg xanthan/kg cell dry mass·h] and molecular weight increased, with dilution rate in the continuous xanthan fermentations. The specific xanthan productivities were not limited by the oxygen transfer rate and were much higher in the continuous cultivations than those predicted by the results of the batch xanthan fermentations.