• 원예과학기술지
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• 제34권1호
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• pp.102-111
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• 2016

본 연구의 목적은 BrDSR(Drought Stress Resistance in B. rapa) 유전자의 기능을 명확히 밝히고, 배추에서 건조 스트레스 반응 유전자들을 분석하는데 있다. 내혼계배추('CT001')와 BrDSR 완전장(438bp의 오픈리딩프레임)을 지닌 pSL100 vector를 재료로 아그로박테리아를 이용한 배추 형질전환을 수행하였다. PCR 분석을 통해 4개체의 형질전환체를 확보하였고, 이들의 BrDSR 발현량은 건조 스트레스 조건에서 비형질전환체 대비 약 1.9-3.4배 정도 더 큰 것으로 분석되었다. 또한 표현형 분석에서도 BrDSR이 과발현된 형질전환체들은 건조 스트레스에 저항성을 보이며 정상적인 생장을 하였다. 기 구축된 건조 스트레스 반응 유전자의 상호발현 네트워크를 기반으로 BrDSR과 밀접한 관련이 있는 유전자들을 분석하기 위해 B. rapa 135K cDNA microarray 데이터를 분석하였다. 그 결과, 환경 스트레스와 관련하여 식물체에서 잎의 노화와 자가소화에 관련된 것으로 보고된 'dark inducible 2(DIN2, AT3G60140)'와 'autophagy 8h(ATG8H, AT3G06420)' 유전자가 확인되었다. 위 결과들을 근거로 BrDSR 유전자는 건조 스트레스에 대한 저항성 향상에 중요한 역할을 할 것으로 판단되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.168-168
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• 2017

Drought conditions during cultivation reduce agricultural production yield less than a theoretical maximum yield under normal condition. Plant specific NAC transcription factors in rice are known to play an essential roles in stress resistance transcriptional regulation. In this study, we report the rice (Oryza sativa L japonica) NAM, AFTF and CUC transcription factor OsNAC17, which is predominantly induced by abiotic stress in leaf, was contribute to the drought tolerance mediated reactive oxygen species (ROS) in transgenic rice plants. Constitutive (PGD1) promoter was introduced to overexpress OsNAC17 and produced the transgenic PDG1:OsNAC17. Overexpression of OsNAC17 throughout the whole plant improved drought resistance phenotype at the vegetative stage. Morphological characteristics such as grain yield, grain filling rate, and total grain weight improved by 22~64% over wild type plants under drought conditions during the reproductive stage. The improved drought tolerance in transgenic rice was involved in reducing stomatal density up to 15% than in wild type plants and in increasing reactive oxygen species-scavenging enzyme. DEG profiling experiment identified 119 up-regulated genes by more than twofold (P<0.01). These genes included UDP-glycosyltransferase family protein, similar to 2-alkenal reductase (NADPH-dependent oxireductase), similar to retinol dehydrogenase 12, Lipoxygenase, and NB-ARC domain containing protein related in cell death. Furthermore, OsNAC17 was act as a transcriptional activator, which has an activation domain in C-terminal region. These result demonstrate that the overexpression of OsNAC17 improve drought tolerance by regulating ROS scavenging enzymes and by reducing stomatal density

• Weed & Turfgrass Science
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• 제4권4호
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• pp.338-347
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• 2015

Reinforcement cells are used to aid grass growth and taken together, this serves to extend greenery beyond the conventional spaces of lawns, tree pits, gardens, and parks, and is advantageous to urban cities since space for greening is often limited. Drought has variable effects on plant life and the resilience of turf to drought resistance also varies with species. Changes in photosynthetic ability were more pronounced for media rather than grass species. The media of sand without organic matter was found to be least suited for drought resistance. Normalized difference vegetation index (NDVI) and digital image analysis (DIA) data were generally in favour of Zoysia species as oppose to A. compressus. In A. compressus, selective traits such as, a more extensive root system and lower specific leaf area (SLA) were not an underlying factor that assisted this grass with enhanced drought resistance. Generally, WUE was found to be strongly related to plant characterises such as overall biomass, photosynthetic features as well as the lushness indexes, and specific leaf area. This study found a strong relationship between WUE and a suite of plant characteristics. These traits should serve as useful selection criteria for species with the ability to resist water stress.

• The Plant Pathology Journal
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• 제29권2호
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• pp.209-220
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• 2013

Root colonization by Pseudomonas chlororaphis O6 induces systemic drought tolerance in Arabidopsis thaliana. Microarray analysis was performed using the 22,800-gene Affymetrix GeneChips to identify differentially-expressed genes from plants colonized with or without P. chlororaphis O6 under drought stressed conditions or normal growth conditions. Root colonization in plants grown under regular irrigation condition increased transcript accumulation from genes associated with defense, response to reactive oxygen species, and auxin- and jasmonic acid-responsive genes, but decreased transcription factors associated with ethylene and abscisic acid signaling. The cluster of genes involved in plant disease resistance were up-regulated, but the set of drought signaling response genes were down-regulated in the P. chlororaphis O6-colonized under drought stress plants compared to those of the drought stressed plants without bacterial treatment. Transcripts of the jasmonic acid-marker genes, VSP1 and pdf-1.2, the salicylic acid regulated gene, PR-1, and the ethylene-response gene, HEL, also were up-regulated in plants colonized by P. chlororaphis O6, but differed in their responsiveness to drought stress. These data show how gene expression in plants lacking adequate water can be remarkably influenced by microbial colonization leading to plant protection, and the activation of the plant defense signal pathway induced by root colonization of P. chlororaphis O6 might be a key element for induced systemic tolerance by microbes.

• 한국작물학회지
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• 제27권1호
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• pp.60-65
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• 1982

맥류의 한발저항성 반응과 정도 그리고 저항생리적 기작을 구명하기 위하여 발아후 10일된 유묘기(본엽 3매시)에 8일간 단수처리를 하여 질산환원효소와 단백질분해효소의 활성변화 그리고 proline축적을 조사하였던 바 그 결과는 다음과 같다. 1. 전품종의 평균 감소율은 질산환원효소의 활성이 42%이였으며 단백질분해효소의 활성은 73%였으며, 이에 반하여 proline은 대조구에 비하여 단수구가 무려 10배나 증가하였다. 2. 질산환원효소의 활성감소율을 맥종별로 보면 소맥 < 호맥 < 대맥 < 2조대맥ㆍ과맥의 순으로 소맥이 가장 낮았다. 3. 단백질분해효소의 활성감소율을 맥종별로 보면 소맥 < 호맥 < 이조대맥 < 대맥 < 과맥의 순으로 소맥이 높고 과맥이 가장 낮았다. 4. 단수구의 proline의 축적색대량을 맥종별로 보면 소맥 < 대맥 < 호맥 < 과맥 < 이조대맥의 순으로 소맥과 대맥이 높았으며, 대조구에 대한 단수구의 증가비는 호맥(13배) > 소맥ㆍ대맥(11배) > 과맥(9배) > 이조대맥(7배)의 순으로 호맥이 가장 높았다. 5. 한발저항성 과정에서 효소적 및 생리적 대사작용의 관점에서 볼 때 소맥 > 호맥 > 대맥 > 과맥 > 이조대맥의 순으로 한발저항성이 강한 것으로 추정된다.

• Molecules and Cells
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• 제41권8호
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• pp.781-798
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• 2018

Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drought stress and flower development. In this study, we isolated and characterized OsWOX13 from rice. OsWOX13 was regulated spatially in vegetative organs but temporally in flowers and seeds. Overexpression of OsWOX13 (OsWOX13-ov) in rice under the rab21 promoter resulted in drought resistance and early flowering by 7-10 days. Screening of gene expression profiles in mature leaf and panicles of OsWOX13-ov showed a broad spectrum of effects on biological processes, such as abiotic and biotic stresses, exerting a cross-talk between responses. Protein binding microarray and electrophoretic mobility shift assay analyses supported ATTGATTG as the putative cis-element binding of OsWOX13. OsDREB1A and OsDREB1F, drought stress response transcription factors, contain ATTGATTG motif(s) in their promoters and are preferentially expressed in OsWOX13-ov. In addition, Heading date 3a and OsMADS14, regulators in the flowering pathway and development, were enhanced in OsWOX13-ov. These results suggest that OsWOX13 mediates the stress response and early flowering and, thus, may be a regulator of genes involved in drought escape.

• 한국자원식물학회지
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• 제23권1호
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• pp.25-30
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• 2010

애기장대의 AtSIZ3(At1g08910) 유전자에 T-DNA를 삽입한 세 종류의 변이형에 저온($4^{\circ}C$), 고온($37^{\circ}C$) 및 건조스트레스를 처리하여 유묘의 생장반응과 유전자 발현을 조사하였다. 저온과 고온처리에 의해서는 야생형과 변이형간에 유묘생장에 유의한 차이를 보이지 않았다. 야생형과 변이형 식물체에 10일간의 건조스트레스를 처리하면 야생형은 재 관수에 의해 모든 식물체가 재생하였으나 변이형은 모두 고사하였고, 10일간의 건조처리로 변이형은 야생형에 비해 유묘생장이 평균 62.9%가 억제되는 것으로 나타났다. 야생형에서 AtSIZ3 유전자는 $4^{\circ}C$의 저온처리에서 무처리를 보다 20%정도 발현이 감소하는 반면, $37^{\circ}C$ 고온처리에서는 3.7배, 건조처리에서는 4.5배가 증가하였다. 이 결과로 보아 AtSIZ3 유전자는 식물의 건조내성과 밀접한 연관이 있을 것으로 판단된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.13-13
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• 2022

Recently, stable crop production is threatened by the effects of climate change. In particular, it is difficult to consistently maintain agricultural policies due to large price fluctuations depending on the difference in total domestic rice production from year to year. For stable rice production amid changes in the crop growing environment, development of varieties with improved disease resistance and abiotic stress stability is becoming more important. In here, drought and cold tolerant trait have been studied. First, for the development of drought tolerant varieties, we analyzed which agricultural traits are mainly affected by domestic drought conditions. As a result, it was observed that drought caused by the lack of water during transplanting season inhibits the development of the number of tiller and reduces the yield. 'Samgang' was selected as a useful genetic resource with strong drought tolerant and stable tiller number development even under drought conditions by phenotype screening. Three of drought tolerant QTLs were identified using doubled haploid (DH) population derived from a cross between Nacdong and Samgang, a drought sensitive and a tolerant, respectively. Among these QTLs, when qVDT2 and qVDTl1 were integrated, it was investigated that the tiller number development was relatively stable in the rainfed paddy field conditions. It is known that the high-yielding Tongil-type cultivars are severely affected by cold stress throughout the entire growth stage. In this study, we established conditions that can test the cold tolerance phenotype with alternate temperature to treat low temperatures in indoor growth conditions similar to those in field conditions at seedling stage. Three cold tolerant QTLs were explored using population derived from a cross between Hanareum2 (cold sensitive variety, Tongil-type) and Unkwang (cold tolerant variety, Japonica). Among these QTLs, qSCT12 showed strong cold tolerant phenotype, and when all of three QTLs were integrated, it was investigated that cold tolerant score was relatively similar to its donor parent, Unkwang, in our experimental conditions. We are performing that development of new variety with improved cold tolerant through the introduction of these QTLs.

• The Plant Pathology Journal
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• 제40권1호
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• pp.30-39
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• 2024

The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of Au. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with Au. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance. Our findings revealed a predominance of downregulated genes in the treated seedlings, suggesting a strategic reallocation of resources to enhance stress defense. Further exploration of enriched Kyoto Encyclopedia of Genes and Genomes pathways and protein-protein interaction networks revealed significant alterations in functional systems known to fortify drought tolerance, including the terpenoid backbone biosynthesis, calcium signaling pathway, pyruvate metabolism, brassinosteroid biosynthesis, and, crucially, flavonoid biosynthesis, renowned for enhancing plant drought resistance. These findings deepen our comprehension of how AK10 biostimulation enhances the resilience of A. koreana to drought stress, marking a substantial advancement in the effort to conserve this endangered tree species through environmentally sustainable treatment.

• Journal of Applied Biological Chemistry
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• 제51권2호
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• pp.50-56
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• 2008

As one way to approach to cold defense mechanism in plants, we previously identified the gene for protein-tyrosine phosphatase (CaTPP1) from hot pepper (Capsicum annuum) using cDNA microarray analysis coupled with Northern blot analysis. We showed that the CaTPP1 gene was strongly induced by cold, drought, salt and ABA stresses. The CaTPP1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Agrobacterium-mediated transformation. The resulting CaTPP1 transgenic tobacco plants showed significantly increased cold stress resistance. It also appeared that some of the transgenic tobacco plants showed increased drought tolerance. The CaTPP1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results showed the involvement of protein tyrosine phosphatase in tolerance of abiotic stresses including cold and drought stress.