• Mycobiology
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• v.31 no.3
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• pp.162-165
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• 2003

Three hundred and sixty five samples of avian droppings, collected from parks and zoo, were investigated for the occurrence of Cryptococcus neoformans in Korea. Thirteen samples were positive for C. neoformans. All isolates were obtained from withered pigeon droppings. Identification and serotyping of isolates were determined by means of serological test and polymerase chain reaction(PCR) fingerprinting. All isolates belonged to C. neoformans var. grubbi(serotype A).

• Journal of Microbiology
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• v.43 no.5
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• pp.469-472
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• 2005

Seventy-two pigeon dropping samples were collected from 26 different localities in Seoul and investigated for the occurrence of Cryptococcus neoformans. Seventeen samples from 8 different localities were found to be positive for C. neoformans. All isolates were obtained from withered pigeon droppings. Identification and serotyping of the isolates were determined by means of serological testing and DNA fingerprinting. All isolates belonged to C. neoformans var. grubbi (serotype A).

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.79-89
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• 2023

This paper is about the development results of an automatic silkworm breeding system to reduce labor and time by automatically performing the works for silkworm droppings changing and feed its food. It consists of an automatic guided vehicle and a processing unit. The automatic guided vehicle transports a silkworm dropping changing frame mounted on a silkworm tray stand, and the processing unit takes over the dropping changing frame on it, removes excrement contained the droppings changing frame and feeds silkworm food. In the case of the current silkworm farming, because the breeding period for large silkworms (4 to 5 stage) is short to 14 days and the supply of mulberry leaves takes 98% of the total amount of mulberry leaves needed for breeding silkworms at this time, labor concentration is intensive, and all breeding works depends on manpower. Therefore, it was difficult to breed large silkworms on a large scale. Moreover, silkworms are bred by adding Silkworm bed (Seop) and mulberry in the silkworm tray, and their droppings changing is to separate silkworms and excrement by moving silkworm trays one by one, and the production cost increases due to the high-cost manpower for silkworm breeding. To solve this problem, technology for automating silkworm breeding has also been developed. However, there is still a limitation that silkworm feeding and droppings changing works are not suitable for mass breeding because a lot of labor and time are spent depending on manual work. Therefore, a new silkworm breeding system for breeding silkworm automatically is needed and so we developed an Automatic Silkworm Breeding System applying the droppings change frame, the inverting unit, the feeding silkworm food device and automatic guided vehicle.

• Mycobiology
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• v.33 no.4
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• pp.188-193
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• 2005

Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 : 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extracellular enzyme activities of the strains by using the API-ZYM system showed two different enzymatic patterns. The genetic variability among C. neoformans isolates was analyzed by random amplified polymorphic DNA (RAPD) using three 10-mer primers. Two different RAPD patterns, which clearly distinguished the isolates, were identified. Analysis of RAPD patterns provided a good characterization of environmental strains of C. neoformans serotype A as a heterogeneous group and were in good agreement with enzymatic profiles.

• Journal of the Korean Applied Science and Technology
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• v.31 no.4
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• pp.663-668
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• 2014

An in-vivo diagnosis of trace Mg(II) ion was performed using a low-cost and environment-friendly voltammetric method, using a graphite counter and reference electrodes and a fluorine-immobilized graphite working electrode, and clean deep seawater was used as an electrolyte solution. Under optimum conditions, the analytical working ranges attained microgram ranges, and a detection limit of $80.6ugL^{-1}$ was obtained using stripping voltammety with 60 sec accumulation time. Ex-vivo application was performed on fish liver and mice droppings. The developed techniques can be applicable to tumor cell analysis.

• 한국약용작물학회:학술대회논문집
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• 2009.05a
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• pp.261-262
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• 2009

• Korean Journal of Environment and Ecology
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• v.27 no.5
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• pp.544-549
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• 2013

We studied the habitat sites of Siberian flying squirrel in Guhak-ri, Sillim-myeon, Wonju-si, Gangwon-do from March to April in 2009. We were found total 30 points of inhabitation trace types; nests 15 points(Tree nest types were 13 points, used Tree hole types hole types were 2 points) and droppings 15 points. We observed 3 individuals of Siberian flying squirrels and founded that 2 individuals of them used tree holes, and another individual used tree nest which were located in branches of Pinus densiflora. Most utilized nest trees of Siberian flying squirrel were Larix leptolepis, and also used Pinus densiflora, Betula davurica, Styrax japonica, Cornus controversa, Acer mono. Droppings trees were Prunus sargentii, Cornus controversa, Quercus xmccormickii, Acer palmatum, Acer palmatum, Betula davurica. Through the observation, we defined that flying squirrels mainly used Conifer trees as nest sites, and decideous trees as droppings.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.4B
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• pp.255-269
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• 2006

The differentiated services(DiffServ) architecture provides packet level service differentiation through the simple and predefined Per-Hop Behaviors(PHBs). The Assured Forwarding(AF) PHB proposed as the assured services uses the RED-in/out(RIO) approach to ensusre the expected capacity specified by the service profile. However, the AF PHB fails to give good QoS and fairness to the TCP flows. This is because OUT(out- of-profile) packet droppings at the RIO buffer are unfair and sporadic during only network congestion while the TCP's congestion control algorithm works with a different round trip time(RTT). In this paper, we propose an Adaptive Regulating Drop(ARD) marker, as a novel dropping strategy at the ingressive edge router, to improve TCP fairness in assured services without a decrease in the link utilization. To drop packets pertinently, the ARD marker adaptively changes a Temporary Permitted Rate(TPR) for aggregate TCP flows. To reduce the excessive use of greedy TCP flows by notifying droppings of their IN packets constantly to them without a decrease in the link utilization, according to the TPR, the ARD marker performs random early fair remarking and dropping of their excessive IN packets at the aggregate flow level. Thus, the throughput of a TCP flow no more depends on only the sporadic and unfair OUT packet droppings at the RIO buffer in the core router. Then, the ARD marker regulates the packet transmission rate of each TCP flow to the contract rate by increasing TCP fairness, without a decrease in the link utilization.

• Biomedical Science Letters
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• v.19 no.3
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• pp.213-223
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• 2013

The objectives of this study are to develop a molecular diagnosis to differentiate serotypes and mating-types of C. neoformans/C. gattii complex isolates from pigeon droppings in Korea and to elucidate molecular epidemiology of the isolates. Phenotypes and genotypes of C. neoformans/C. gattii complex isolates were identified by biochemical properties and PCR using specific CNLAC1 gene, respectively. To classify serotypes and mating-types of C. neoformans/C. gattii complex isolates, the five reference strains and thirty-three isolates in Korea were investigated by restriction fragment length polymorphism (RFLP) analysis using CNLAC1 gene for varieties, by random amplified polymorphic DNA (RAPD) for serotyping, and by PCR using specific primer sets for mating typing. All isolates in Korea were belonged to C. neoformans var. grubii (serotype A) by RFLP and RAPD patterns which showed high sensitivity and specificity. Therefore, RFLP and RFLP were available to differentiate varieties and serotypes of C. neoformans. Amplification patterns of the five reference strains by specific PCR for mating typing were differentiable, and all isolates were classified into $MAT{\alpha}$. All C. neoformans environmental isolates in Korea were Cr. neoformans serotype A and $MAT{\alpha}$ which is a more virulent pathogen. This study suggests that RFLP and RAPD are rapid and correct molecular diagnosis tools for epidemiology of C. neoformans/C. gattii complex isolates.

• Journal of the Korean Applied Science and Technology
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• v.29 no.4
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• pp.626-630
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• 2012

Diagnosis with an ex-vivo gold sensor was done using a modified fluorine-doping sensor, and cyclic voltammetry (CV) redox potentials of 0.4 V anodic and -0.2 V cathodic were obtained. Both peak currents were optimized using square-wave (SW) stripping voltammetry, and an analytical working range of 10-80 ug/L SW was attained. The precision of the 10-mg/L Au was 0.765 (n=8) RSD under the optimum conditions, and the analytical detection limit approached 0.006 ug/L (S/N=3) with only a 60 sec accumulation time. The developed method was used to examine the mouse droppings for medicinal diagnosis.