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Dot-Blot Immunoassay of Fasciola gigantica Infection using 27 kDa and Adult Worm Regurge Antigens in Egyptian Patients

  • Kamel, Hanan H.;Saad, Ghada A.;Sarhan, Rania M.
    • Parasites, Hosts and Diseases
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    • v.51 no.2
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    • pp.177-182
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    • 2013
  • The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to assess this assay as a practical tool for diagnosis fascioliasis in Egyptian patients. Fasciola gigantica antigen of an approximate molecular mass 27- (KDa) was obtained from adult worms by a simple elution SDS-PAGE. A Dot-Blot was developed comparatively to adult worm regurge antigens for the detection of specific antibodies from patients infected with F. gigantica in Egypt. Control sera were obtained from patients with other parasitic infections and healthy volunteers to assess the test and compare between the antigens. The sensitivity, specificity, positive and negative predictive values of Dot-Blot using the adult worm regurge were 80%, 90%, 94.1%, and 69.2% respectively, while those using 27-KDa were 100% which confirms the diagnostic potential of this antigen. All patients infected with Fasciola were positive, with cross reactivity reported with Schistosoma mansoni serum samples. This 27-KDa Dot-Blot assay showed to be a promising test which can be used for serodiagnosis of fascioliasis in Egyptian patients especially, those presenting with hepatic disease. It is specific, sensitive and easy to perform method for the rapid diagnosis particularly when more complex laboratory tests are unavailable.

Studies on the mycoplasmal pneumonia in slaughter pigs. 1. Seasonal detection by gross finding of lung lesion and dot-ELISA technique (도축돈의 마이코플라즈마성 폐렴에 관한 연구 1. 육안적 폐병변과 dot-ELISA에 의한 계절별 조사)

  • Lim, Young-Taek;Seok, Ho-Bong
    • Korean Journal of Veterinary Research
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    • v.42 no.2
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    • pp.219-224
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    • 2002
  • We report the seasonal prevalence of the mycoplasmal pneumoniae of swine (MPS) in slaughter pigs from July of 1999 to June of 2000. Gross finding of lung lesion observed and examined by dot-ELISA. In gross finding of lung lesion from 750 pig samples, 465 (62.0%) was MPS, and 129 (17.2%) was single or double infection with actinobacillosis and pasturellosis. However, 156 (20.8%) had no lesion. In seasonal detection, the prevalence was found to be winter (69.5%), autumn (63.5%), summer (60.0%) and spring (54.7%) in orderly frequency. In dot-ELISA, the result was showed the positive reaction (x16>titre) with 58.0% and negative (x4

Study of the Characteristics of Dot Pattern Designs in Modern Fashion (현대패션에 나타난 도트문양의 표면유형과 특성에 관한 연구)

  • Kim, Sun-Young
    • Journal of the Korean Society of Costume
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    • v.59 no.4
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    • pp.41-53
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    • 2009
  • This study analyzed expression types of dot patterns and derived out inherent characteristics to provide fundamental resources for advancement of high value added creative designs. As a result of the study, Firstly, the circular motive was used to form silhouettes or as a symbol of a decoration. Secondly, a simple form and color dot pattern was used to create one side, or a circular shaped accessory was used to be recognized as a construction line or a decoration line. Thirdly, textile printing is mainly used but handicraft and decorative images were used to add vitality through piece technique, embroidery, collage, cut-out, patch work, etc. Fourthly, different circular motives were integrated, partitioned and duplicated for abstract geometrical images. Fifthly, variations were added by mixing different dot patterns that are arranged regularly and irregularly in different sizes and gaps creating compounded designs with handicraft touches, different angles or on top of each other. Sixthly, hybrid images were created with rearrangement of dot patterns and by adding floral shapes, stripes or other abstract and geometrical shapes. Such various and creative attempts construct new formative beauty in fashion design and I believe that it can establish the development of unique images that satisfies the taste of today's consumers.

Experimental Study on the Influence of Dot Geometry on Ink Transfer in Gravure Printing (망점의 크기와 형상이 잉크 전이에 미치는 영향에 관한 실험적 연구)

  • Han, Kyung-Joon;Song, Hyun-Min;Ahn, Byung-Joon;Ko, Sung-Lim
    • Journal of the Korean Society for Precision Engineering
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    • v.28 no.10
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    • pp.1123-1130
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    • 2011
  • The pattern shape engraved on the gravure printing roll is one of the most important factors influencing ink transfer. This study focuses on the relations between dot geometry engraved on gravure printing roll and the ink transfer during the gravure printing process. The influence of dot width on printed patterns will be demonstrated. Results reveal that as the width of a dot on the printing roll increases, the ink transfer rate also increases. But over a certain size of width, surface uniformity began to recede. Therefore, proper dot geometry on the printing roll should be decided to guarantee good printing quality according to printing conditions and expected performance of the electronic devices.

Research Trend for Quantum Dot Quantum Computing (양자점 큐비트 기반 양자컴퓨팅의 국외 연구 동향 분석)

  • Baek, Chungheon;Choi, Byung-Soo
    • Electronics and Telecommunications Trends
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    • v.35 no.2
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    • pp.79-88
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    • 2020
  • Quantum computing is regarded as one of the revolutionary computing technologies, and has attracted considerable attention in various fields, such as finance, chemistry, and medicine. One of the promising candidates to realize fault tolerant quantum computing is quantum dot qubits, due to their expectation of high scalability. In this study, we briefly introduce the international tendencies for quantum dot quantum computing. First, the current status of quantum dot gate operations is summarized. In most systems, over 99% of single qubit gate operation is realized, and controlled-not and controlled-phase gates as 2-qubit entangling gates are demonstrated in quantum dots. Second, several approaches to expand the number of qubits are introduced, such as 1D and 2D arrays and long-range interaction. Finally, the current quantum dot systems are evaluated for conducting quantum computing in terms of their number of qubits and gate accuracies. Quantum dot quantum computing is expected to implement scalable quantum computing. In the noisy intermediate-scale quantum era, quantum computing will expand its applications, enabling upcoming questions such as a fault-tolerant quantum computing architecture and error correction scheme to be addressed.

New Records of Five Ennomine Moths (Lepidoptera: Geometridae; Ennominae) from Korea

  • Choi, Sei-Woong;Kim, Sung-Soo
    • Animal Systematics, Evolution and Diversity
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    • v.31 no.2
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    • pp.122-127
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    • 2015
  • The subfamily Ennominae is one of the most species-rich taxa of Geometridae that include more than 9,700 species worldwide and over 280 species in South Korea. Herein, we present the first report of five species of Ennominae. Abraxas flavisinuata can be characterized by the white wings, a thick black postmedial line that is medially and dorsally broken with an orange band, large rounded black discal dots on the forewing, and a black dotted postmedial line on the hindwing. Lomographa claripennis can be characterized by the whitish wings, the black undulating postmedial line as well as the minute blackish discal dot on the forewing, and the black undulating postmedial line with a minute black discal dot on the hindwing. Arichanna tetrica can be characterized by the grayish forewings, thick black transverse ante- and postmedial lines, a large blackish discal dot, whitish apical streak on the forewing, and scattered black dots on the whitish hindwing. Apocleora rimosa can be characterized by the brown ground color of the fore- and hindwings, the black slanted ante- and postmedial lines of the forewing, and two black medial lines on the hindwing. Ourapteryx japonica can be characterized by the white wings, the dark brown transverse ante- and postmedial lines with a long discal dot on the forewing, and the dark brownish transverse antemedial line as well as a termen that has a sharp white tail with one large dark reddish dot and one small black dot on the hindwing.

Physiological Responses to Maximal Exercise Loading in Spinal Cord Injured Paraplegia (척수손상으로 인한 하반신마비 환자의 최대운동부하시 생리학적 반응)

  • Yu, Byong-Kyu;Chung, Nak-Su
    • Physical Therapy Korea
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    • v.6 no.2
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    • pp.56-66
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    • 1999
  • The purpose of this study focused how to show physiological responses comparing exercise group and non exercise group for progressive maximal wheelchair ergometer exercise loading in complete paraplegia. It also examined the various factors which would be influenced physiological responses. Sixteen subjects have been investigated in this study, and the subjects are divided into two groups as follows: 1) exercise group (7 subjects) 2) non exercise group (9 subjects). Each test was terminated by physical exhaustion and/or an inability to maintain a flywheel velocity. The results were as follows: 1) No difference was noted in pulmonary function test between two groups. 2) $\dot{v}$ Emax value during maximal exercise was significantly different between the groups (p<0.05). The mean $\dot{v}$ Emax of exercise group was $69.67{\ell}/min$, non exercise group was $41.47{\ell}/min$. 3) $\dot{v}$ $O_2max$(${\ell}/min$) value during maximal exercise was significantly different between the groups (p<0.05). The mean $\dot{v}$ $O_2max$(${\ell}/min$) of exercise group was $1.72{\ell}/min$, non exercise group was $1.15{\ell}/min$. 4) $\dot{v}$ $O_2$ max(ml/kg/min) value during maximal exercise was significantly different between the groups (p<0.05). The mean $\dot{v}$ $O_2max$($ml/kg{\cdot}min$) of exercise group was $25.99ml/kg{\cdot}min$, non exercise group was $18.61{\ell}/min$. 5) Maximal heart rate(HRmax) value during maximal exercise was significantly different between the groups (p<0.05). The mean HRmax of exercise group was 180.43 beats/min, non exercise group was 175.00 beats/min. 6) $\dot{v}\;E/\dot{v}\;O_2$ value during maximal exercise was not significantly different between the groups (p>0.05). The mean $\dot{v}\;E/\dot{v}\;O_2$ of exercise group was $36.36{\ell}/{\ell}\;O_2$, non exercise group was $45.46{\ell}/{\ell}\;O_2$. Considering the results which explore the exercise group with paraplegia has shown the maximal aerobic power compared with non exercise group, regular and consistent physical training is highly assumed as a main factor to improve cardiopulmonary fitness.

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The Effect of Graphical Formats on Computer-Based Idea Generation Performance

  • Jung, Joung-Ho
    • The Journal of Information Systems
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    • v.27 no.1
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    • pp.153-169
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    • 2018
  • Purpose Since human brains catch images faster than texts or numbers, infographics has been widely used in business in the form of "information dashboard" to enhance the efficiency of decision-making. Groupware, however, has neglected the adoption and use of infographics, in particular, in the idea generation process. Given that an overall performance of groupware-based idea generation is no better than that of the (paper-and-pencil-based) Nominal Group Technique, Jung et al. (2010) adopted the notion of infographics in the form of performance feedback to solve the productivity paradox. With the consistent results, which demonstrate beneficial effects of infographics on performance enhancement, an interesting observation that groups with the bar chart treatment performed better than groups with the dot chart treatment was made. The main purpose of this study was to find if there were a performance consistency between the outcomes from the previous study and the outcomes from the current study. Design/methodology/approach In experiment 1, we employed the same system used in the previous study (i.e., Jung et al., 2010). As individuals' contributions accumulated, the mechanism visually displayed individuals' performances two-dimensionally in the form of a bar chart or a dot chart. Then, we compared the performance outcomes from this study to the outcomes from previous study (i.e., Jung et al., 2010). In experiment 2, we modified the performance graph to test the effect of "playfulness" on performance by converting dots to car images. Then, we compared the performance outcome from experiment 2 to the outcomes from experiment 1. Findings Just like our interesting (and unexpected) finding in Jung et al.'s study (2010), the outcome confirmed a consistent superior performance of a bar chart. This implies that a bar chart is a better choice when stimulating performance with a visual aid in the context of groupware-based idea generation. Although a bar chart was criticized in a way that errors of length-area judgments are 40 ~ 250% greater than those of positional judgments along a common scale, such illusion turned out to be facilitating upward performance comparison better. Regarding Experiment 2, the outcome showed that the revised-dot graph is as good as the bar graph in terms of quantity and quality score of ideas. We attribute the performance enhancement of the resized-dot to the interaction between the motivational characteristic and the situational characteristic of playfulness because individuals in the revised-dot graph treatment performed better than individuals in the dot graph treatment. Given the order of performance (Bar >= Revised Dot > Dot) that the revised-dot treatment performed the same as (or lower than) the bar treatment, an additional research is warranted to reach to a consistent outcome.

An Efficient Spatial Join Method Using DOT Index (DOT 색인을 이용한 효율적인 공간 조인 기법)

  • Back, Hyun;Yoon, Jee-Hee;Won, Jung-Im;Park, Sang-Hyun
    • Journal of KIISE:Databases
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    • v.34 no.5
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    • pp.420-436
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    • 2007
  • The choice of an effective indexing method is crucial to guarantee the performance of the spatial join operator which is heavily used in geographical information systems. The $R^*$-tree based method is renowned as one of the most representative indexing methods. In this paper, we propose an efficient spatial join technique based on the DOT(Double Transformation) index, and compare it with the spatial Join technique based on the $R^*$-tree index. The DOT index transforms the MBR of an spatial object into a single numeric value using a space filling curve, and builds the $B^+$-tree from a set of numeric values transformed as such. The DOT index is possible to be employed as a primary index for spatial objects. The proposed spatial join technique exploits the regularities in the moving patterns of space filling curves to divide a query region into a set of maximal sub-regions within which space filling curves traverse without interruption. Such division reduces the number of spatial transformations required to perform the spatial join and thus improves the performance of join processing. The experiments with the data sets of various distributions and sizes revealed that the proposed join technique is up to three times faster than the spatial join method based on the $R^*$-tree index.

The Basic Study on the Preventive Measures against Medical Accident Induced by Acupuncture and Moxibustion Therapy I. A Literature Research on the Essence of Prohibitive Acupuncture Point, Jīn-Zhėn-Xué-Gė (禁鍼穴歌) (침구 의료행위에 기인하는 의료사고 예방에 관한 기초적 연구 (I) - 금침혈가에 관한 문헌적 고찰 -)

  • Moon, Jin-Young
    • The Journal of Dong Guk Oriental Medicine
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    • v.8 no.2
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    • pp.175-199
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    • 2000
  • Acupuncture and moxibustion play an important role not only in the medical service and health care of Korea, China and japan but also in those of many western countries such as Germany, France, United States of America. An unexpected results induced by careless acupuncture and moxibustion treatments may be mild or severe, and are due to practitioner's mistake and particular body condition of a patient. This study was performed to establish a preventive measures against medical accident induced by acupuncture therapy. Total 23 acupuncture points which are included in a J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ were selected as prohibited acupuncture points. Although the records in J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ are an important data historically which show dangerous acupuncture points, there are few a full investigation on J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ up to the present. So present study was done for literature review to understand a feasibility of the records in J${\bar{l}}$n-Zh${\dot{e}}$n-Xu${\acute{e}}$-G${\dot{e}}$ in association with dangerous acupuncture points. Based upon the locations of these acupuncture points, the dangerous region were classified three categories, head, soma and limb. And abnormal signs related to the improperly performed acupuncture treatment on each point were investigated by using historical reference books on acupuncture and moxibustion. And then names, locations, depth of insertion, operation methods and side effects of each point were investigated.

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