• Asian Pacific Journal of Cancer Prevention
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• v.16 no.12
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• pp.4915-4920
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• 2015

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

• Korean Journal of Environmental Agriculture
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• v.13 no.1
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• pp.98-110
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• 1994

The fauna of spider species inhabited near paddy fields have been investigated in Chinju and Namhae areas. The spiders collected near the paddy fields in Chinju and Namhae areas were 34 species of 31 genera belonging to 10 families: 34 species on paddy levees; 16 species on barley fields; 17 species on fallow fields; and 18 species on paddy fields. The species diversity was the highest on paddy levee. The dominant species collected from each habitat near the paddy fields were Pardosa astrigera on the paddy levees and barley fields, Pirata subpiraticus on the fallow field, and Pachygnatha clercki on the paddy fields. The spiders inhabited near rice fields can be classified into nine groups based on the ecological and taxonomical characteristics: 1. Pirata spp. 2. Pachygnatha clercki 3. Teridiidae, Erigonidae, Linypidae 4. Tetragnatha spp. 5. Dolomedes surfureus 6. Pardosa spp. 7. Salticidae 8. Thomisidae 9. Clubionidae Among the nine spider groups, the spiders belong to the group 1-5 prefered wet ground or paddy to dry ground, in contrast to the group 6-9 which prefered to live on dry land. The difference of the habitat preference indicated that the former five groups do the important roll as pest predators in paddy, while the latter four group mainly suppress the overwintering pest populations on paddy levees.

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.1-7
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• 1996

In order to get basic information for establishing weed control methods in greenhouse, weed distribution was surveyed at 42 greenhouses in 10 cities and counties of Gyeongnam and Junnam area from September to December, 1995. Sixty nine weed species in 23 families which were composed of 28 annuals, 16 biennials and 25 perennials were identified. Broadleaf weed species was 50 species, followed by 8 species in grasses, and 11 species in sedges. Cruciferae was the most widely occurring family belong to 9 species, followed by 8 species in Compositae, Cyperaceae and Gramineae, respectively, 6 species in Polygonaceae, and the other 17 families have 1~3 species. The dominant weed species occurred in greenhouses based on summed dominance ratio of weeds were Cardamine flexuosa var. fallax, Digitaria sanguinalis, Eleusine indica, Stellaria aquatica, Centipeda minima, Mollugo pentaphylla, Portulaca oleracea, Rorippa islandica.

• Weed & Turfgrass Science
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• v.4 no.3
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• pp.188-198
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• 2015

This study was conducted in order to investigate the distribution pattern of weeds and ecological characteristics on the dry field in the Eastern region of Korea. The weed species data were obtained from 12 regions in Gangwon-do and Gyeongsang-do. 150 taxa were recorded from three main distinguished habitats; inside of dry field (IDF), embankment around the end of dry field (EDF), levee slope of dry field (LS). The weed species of three different habitats were IDF 83 taxa, EDF 133 taxa and LS 105 taxa. The species showing higher rNCD value was Portulaca oleracea in IDF, Digitaria sanguinalis in EDF and Humulus japonicus in LS. Compositae was dominant family followed by Gramineae, Polygonaceae and Leguminosae. Life form was distinguished as Th-R5-D4-e and three different habitats showed the same results. Disturbance index was analysed as IDF 83.1%, EDF 68.4%, and LS 62.9%. Naturalized rate was analysed as IDF 28.9%, EDF 22.6%, and LS 20.0%. These results indicate that the weeding management, land use intensity, and human interference effect on the distribution index and naturalized rate of weed.

• Microbiology and Biotechnology Letters
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• v.33 no.3
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• pp.207-214
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• 2005

To characterize the genotypic traits of clinical methicillin-resistant Staphyiococus aureus (MRSA) isolates (n=49), major virulence-associated genes were detected by using PCR-based methods. All the MRSA isolates possessed coagulase gene and showed four polymorphism types [500bp ($6{\%}$),580bp ($27{\%}$), 660bp ($65{\%}$) and 740bp ($2{\%}$)] due to variable numbers of tandem repeats present within the gene. The four or five different loci of hemolysin gene family were dominant in the MRSA isolates,25 of which($51{\%}$) possessed a combination of hla / hlb / hld/ hlg / hlg-2 genes as the most prevalent type. The prevalence of enterotoxin genes was varied among the MRSA isolates. sea and seb genes were detected from all the MRSA isolates. But sei, tsst-1, seg, sec, and seh genes were detected from 31 ($63{\%}$), 16 ($33{\%}$), 14 ($29{\%}$), 8 ($16{\%}$), and 5 ($10{\%}$) isolates, respectively. sed and sej genes were detected from 1 ($2{\%}$) isolate, respectively. see, eta, and etb genes were not detected at all. sea / seb genes were co-detected from 11 ($23{\%}$) isolates, sea / seb / sei genes from 9 ($19{\%}$) isolates, and sea / seb / seg / sei / tsst-1 genes from 5 ($10{\%}$) isolates. Other genes were co-detected with below $10{\%}$ frequencies.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.957-966
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• 2017

Objective: To understand the dynamic structure, function, and influence on nutrient metabolism in hosts, it was crucial to assess the genetic potential of gut microbial community in yaks of different ages. Methods: The denaturing gradient gel electrophoresis (DGGE) profiles and Illumina-based metagenomic sequencing on colon contents of 15 semi-domestic yaks were investigated. Unweighted pairwise grouping method with mathematical averages (UPGMA) clustering and principal component analysis (PCA) were used to analyze the DGGE fingerprint. The Illumina sequences were assembled, predicted to genes and functionally annotated, and then classified by querying protein sequences of the genes against the Kyoto encyclopedia of genes and genomes (KEGG) database. Results: Metagenomic sequencing showed that more than 85% of ribosomal RNA (rRNA) gene sequences belonged to the phylum Firmicutes and Bacteroidetes, indicating that the family Ruminococcaceae (46.5%), Rikenellaceae (11.3%), Lachnospiraceae (10.0%), and Bacteroidaceae (6.3%) were dominant gut microbes. Over 50% of non-rRNA gene sequences represented the metabolic pathways of amino acids (14.4%), proteins (12.3%), sugars (11.9%), nucleotides (6.8%), lipids (1.7%), xenobiotics (1.4%), coenzymes, and vitamins (3.6%). Gene functional classification showed that most of enzyme-coding genes were related to cellulose digestion and amino acids metabolic pathways. Conclusion: Yaks' age had a substantial effect on gut microbial composition. Comparative metagenomics of gut microbiota in 0.5-, 1.5-, and 2.5-year-old yaks revealed that the abundance of the class Clostridia, Bacteroidia, and Lentisphaeria, as well as the phylum Firmicutes, Bacteroidetes, Lentisphaerae, Tenericutes, and Cyanobacteria, varied more greatly during yaks' growth, especially in young animals (0.5 and 1.5 years old). Gut microbes, including Bacteroides, Clostridium, and Lentisphaeria, make a contribution to the energy metabolism and synthesis of amino acid, which are essential to the normal growth of yaks.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.247-252
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• 2015

Aronia (Black chokeberry, Aronia melanocarpa) belonging to the Rosaceae family, is native to eastern North America. Aronia contain high levels of flavonoids, mostly anthocyanins and proanthocyanidins, which are known as condensed tannins. The dominant proanthocyanidins in aronia are (-)-epicatechin and (+)-catechin. The concentration of proanthocyanidins in aronia is higher than in other berries, however due to the astringent taste it is not desirable for consumption. Therefore, the purpose of this study is to evaluate the effect of aronia on the reduction in tannins by yeast isolated from regional Jeotgal. We isolated strains of yeast with high ${\beta}$-glucosidase activity from Jeotgal, with the MTY2 strains exhibiting a reduction in final tannin concentration according to thin layer chromatography (TLC) analysis. MTY2 was confirmed as Kazachstania servazzii using an 18S rDNA sequence and named as K. servazzii MTY2. K. servazzii MTY2 showed most significant growth when K. servazzii MTY2 was cultured in a solution of 10% (w/v) glucose, 3% (w/v) tryptone and 0.1% (w/v) sodium chloride. According to the high performance liquid chromatography (HPLC) analysis, the (+) - catechin peak is present, but (-) - epicatechin peak was reduced at culture condition added with 10% glucose in medium.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.444-450
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• 2013

A Gram-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated FW-$6^T$ was isolated from a freshwater sample and its taxonomic position was investigated by using a polyphasic approach. Strain FW-$6^T$ grew optimally at $10-42^{\circ}C$ and at pH 7.0 on nutrient and R2A agar. Strain FW-$6^T$ displayed ${\beta}$-glucosidase activity that was responsible for its ability to transform ginsenoside $Rb_1$ (one of the dominant active components of ginseng) to Rd. On the basis of 16S rRNA gene sequence similarity, strain FW-$6^T$ was shown to belong to the family Sphingomonadaceae and was related to Novosphingobium aromaticivorans DSM $12444^T$ (98.1% sequence similarity) and N. subterraneum IFO $16086^T$ (98.0%). The G+C content of the genomic DNA was 64.4%. The major menaquinone was Q-10 and the major fatty acids were summed feature 7 (comprising $C_{18:1}{\omega}9c/{\omega}12t/{\omega}7c$), summed feature 4 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}2OH$), $C_{16:0}$, and $C_{14:0}$ 2OH. DNA and chemotaxonomic data supported the affiliation of strain FW-$6^T$ to the genus Novosphingobium. Strain FW-$6^T$ could be differentiated genotypically and phenotypically from the recognized species of the genus Novosphingobium. The isolate that has ginsenoside converting ability therefore represents a novel species, for which the name Novosphingobium ginsenosidimutans sp. nov. is proposed, with the type strain FW-$6^T$ (= KACC $16615^T$ = JCM $18202^T$).

• Korean journal of applied entomology
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• v.44 no.4 s.141
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• pp.307-315
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• 2005

In this study we tried to develop a biological method for evaluating environment using the potter wasps as an indicator. Wasp species in family Eumenidae, were collected from 13 locations including agricultural area using nest trap. The wasps collected were classified and selected candidate species as an environmental evaluation indicator. Seven species were collected and the Anterhynchium flavomarginatum was the most dominant species. The number of the nest of potter wasps was 12.8/m both in the non-fertilizer and the non-pesticide areas and 7.2/m in the general agricultural areas. The number of nests was 13.4/m in the location where the Degree of Green Naturality (DGN) was high (4.28) and it was 1.2/m where the DGN was low (1.00), suggesting that the index of both richness and diversity tend to increase in locations with more nests. Based on these results, A. flavomarginatum, Orancistrocerus drewseni (Saussure), Isodontia nigellus and Chalicodoma sculpturalis were selected as indicator species for the evaluation of environment including agricultural ecosystem. And a standard for grading an environment (I to IV) was made based on the occurrence, the total number of nesting and the species diversity of potter wasps.

• Korean Journal of Hospice Care
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• v.5 no.1
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• pp.14-25
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• 2005

This study was conducted to examine the present state, types of services, difficulties, education programs, motivation, satisfaction and attitude of hospice volunteers and also to suggest supporting programs for the volunteer. For these purposes, a questionnaire was distributed among the 200 hospice volunteers in ten hospice organizations in Daejeon and Chungcheong province. In this research, 85.5% of the respondents were women and most of them were housewives in their forties and fifties. The majority of hospice volunteers had an education background of more than high school. Christianity (94%) was the dominant religion of volunteers. Among the service area of the hospice, emotional caring, such as listening closely and establishing empathy were the most frequently done by the respondents and the next one was spiritual care. Hospice volunteers who had started with altruistic motivation show more dynamic activities in emotional, spiritual, physical caring and so on, than those with non-altruistic motivation. Most of the respondents expressed that they felt a hospice volunteer was valuable. However they had a hard time to overcome the sorrow coming from sharing empathy with the dying patients. Those who had started with altruistic motivation usually took part in two times per a week and had more positive attitude. Volunteers who had been working more than five years showed better attitude than those who had been working less than three years. The longer the voluntary services, the more positive attitude experienced by volunteers. Hospice volunteers encountered the most considerable difficulty with lack of background knowledge and their actual capability. Insufficiency of time was the next reason for the considerable difficulty of the hospice volunteer. On the other hand, those who served more than twice per week replied that lack of background knowledge and their capability were the least considerable difficulty. Insufficiency of time was the reason of difficulty for male volunteers, as well as for females under thirty and those in their forties and fifties respectively. Generally most of the hospice volunteers (70%) were satisfied with their services and they usually satisfied with physical caring, spiritual caring, hospice's family caring. The less difficulty in voluntary services, more satisfaction for the volunteers in the end. There is a positive correlation between a attitude of the hospice volunteer and frequency of voluntary services while the volunteers' difficulty affect negatively their attitude as well as the frequency of voluntary services. Most of the hospice volunteers want to have more education about basic skills, volunteer's attitude and role, spirituality, the way of attending on the hospice and so on. Most of them considered discussion and sharing different cases as the most effective method. They also wish to had more supporting programs for the hospice volunteers(in the order of their needs) such as regular events, newsletters, personal concern, social meetings among the volunteers, insurance and minimum expenses. Based on the study results, more programs should be run in order to activate the voluntary services regardless of their gender and education background. A continuous practical supporting policy and education programs are required in order to provide special education and training courses considering every field of voluntary services. Expanding the role of the volunteers allows them more opportunities to take part in voluntary services and th activate participation. It is necessary to establish a new hospice system as a part public medical system, which can not only facilitate the voluntary services for a hospice but also enhance professional hospice volunteers. Finally, experts are needed to operate the voluntary services effectively.