• Title/Summary/Keyword: domain sequence

Search Result 773, Processing Time 0.026 seconds

Image Retrieval using Adaptable Weighting Scheme on Relevance Feedback (MPEG-2 비디오 스트림 편집시의 비트율 제어)

  • 윤범식;노홍진;최윤식
    • Journal of Broadcast Engineering
    • /
    • v.5 no.2
    • /
    • pp.188-198
    • /
    • 2000
  • As MPEG-2 is introduced, a new editing system that works at the compressed domain is required. It is efficient to paste the two different video sequences in the compressed domain, but after repeated pasting, the result sequence can violate MPEG-2 rate control algorithm. In other words, it causes the underflow of the buffer during the decoding time. The earlier study inserted an additional fade-in sequence but it is not natural when we make a program. In this paper, a nobel method using requantization of the underflow sequence is presented.

  • PDF

Kinetic analysis of Drosophila Vnd protein containing homeodomain with its target sequence

  • Yoo, Si-Uk
    • BMB Reports
    • /
    • v.43 no.6
    • /
    • pp.407-412
    • /
    • 2010
  • Homeodomain (HD) is a highly conserved DNA-binding domain composed of helix-turn-helix motif. Drosophila Vnd (Ventral nervous system defective) containing HD acts as a regulator to either enhance or suppress gene expression upon binding to its target sequence. In this study, kinetic analysis of Vnd binding to DNA was performed. The result demonstrates that DNA-binding affinity of the recombinant protein containing HD and NK2-specific domain (NK2-SD) was higher than that of the full-length Vnd. To access whether phosphorylation sites within HD and NK2-SD affect the interaction of the protein with the target sequence, alanine substitutions were introduced. The result shows that S631A mutation within NK2-SD does not contribute significantly to the DNA-binding affinity. However, S571A and T600A mutations within HD showed lower affinity for DNA binding. In addition, DNA-binding analysis using embryonic nuclear protein also demonstrates that Vnd interacts with other nuclear proteins, suggesting the existence of Vnd as a complex.

ON SEQUENCE SPACES DEFINED BY THE DOMAIN OF TRIBONACCI MATRIX IN c0 AND c

  • Yaying, Taja;Kara, Merve Ilkhan
    • Korean Journal of Mathematics
    • /
    • v.29 no.1
    • /
    • pp.25-40
    • /
    • 2021
  • In this article we introduce tribonacci sequence spaces c0(T) and c(T) derived by the domain of a newly defined regular tribonacci matrix T. We give some topological properties, inclusion relations, obtain the Schauder basis and determine ��-, ��- and ��- duals of the spaces c0(T) and c(T). We characterize certain matrix classes (c0(T), Y) and (c(T), Y), where Y is any of the spaces c0, c or ℓ∞. Finally, using Hausdorff measure of non-compactness we characterize certain class of compact operators on the space c0(T).

Multiple Functions of the Amino-terminal Domain of Bacteriophage Lambda Integrase: A New Member of Three-stranded $\beta-sheet$ DNA-binding Proteins

  • Cho Eun Hee
    • Proceedings of the Microbiological Society of Korea Conference
    • /
    • 2002.10a
    • /
    • pp.159-161
    • /
    • 2002
  • Bacteriophage lambda integrase carries out the site-specific recombination of lambda. Integrase contains two DNA binding domains with distinct sequence specificity, namely arm-type binding and core-type binding domains. The amino-terminal arm-binding domain is structurally related to the three-stranded $\beta-sheet$ family of DNA-binding domains. Integrase binding to the high affinity arm-type site by the amino-terminal domain facilitates Int binding to the low affinity core-type site, where the cleavage and strand exchange occurs. The amino-terminal domain of Int also modulates the core-binding and catalysis through intramolecular domain-domain interaction and/or intermolecular interactions between Int monomers. In addition, the amino-terminal domain interacts cooperatively with excisionase during excision. This indicates that amino-terminal domain of Int plays an important role in formation of proper higher-order nucleoprotein structure required for lambda site-specific recombination.

  • PDF

Multiple Facets of Sox Gene (SOX 유전자의 다양성)

  • 홍경원;김희수
    • Journal of Life Science
    • /
    • v.14 no.4
    • /
    • pp.716-725
    • /
    • 2004
  • Sox protein family, a transcription factor, has been found in whole animal kingdom, and contains a sequence-specific DNA binding domain called high mobility group domain (HMG). The Sox protein family based on the amino acid sequence of HMG domain was classified into 10 groups. Each group of Sox family shows significant conservation from nematode to human. The HMG domain affect to various developmental cell differentiation through binding to enhancer and regulating other transcription factors. Recently, many molecular biologists focus their research on the illustration of Sox-related disease, evolution and phylogeny. Especially, stem cell research with Sox gene family is indispensable field for understanding of their biological functions. The understanding of Sox genes may contribute to understand their role in human genetic disease and whole animal evolution.

A Low Complexity SLM-PRSC Hybrid Scheme for OFDM PAPR Reduction (OFDM 신호의 PAPR 감소를 위한 저복잡도의 SLM-PRSC 결합 기법)

  • Han, Seung-Woo;Yang, Suck-Chel;Shin, Yo-An
    • Proceedings of the IEEK Conference
    • /
    • 2006.06a
    • /
    • pp.21-22
    • /
    • 2006
  • In this paper, to improve OFDM PAPR reduction performance of the conventional SLM method, we propose an effective SLM-PRSC hybrid scheme based on the repeated utilization of identical PRSC sequences in time domain. In the proposed scheme, after performing the SLM for the frequency domain OFDM symbol excluding the pre-determined PRSC positions, the final SLM-PRSC hybrid sequence with the lowest PAPR, which is generated by adding the time domain PRSC sequence to the results of the SLM is selected as the transmitted OFDM symbol. In particular, since the identical PRSC sequences generated a priori are repeatedly used for every OFDM symbol, excessive IFFT calculations are avoided. Moreover, the selected PRSC symbols in the frequency domain may be utilized as an overhead information for the SLM. Simulation results reveal that the proposed SLM-PRSC hybrid scheme can remarkably improve the PAPR reduction performance of the conventional SLM, while avoiding excessive increase of IFFT and PAPR calculations and the overhead for the SLM.

  • PDF

cDNA Cloning, Expression and Homology Modeling of a Luciferase from the Firefly Lampyroidea maculata

  • Emamzadeh, Abdo Rahman;Hosseinkhani, Saman;Sadeghizadeh, Majid;Nikkhah, Maryam;Chaichi, Mohammad Javad;Mortazavi, Mojtaba
    • BMB Reports
    • /
    • v.39 no.5
    • /
    • pp.578-585
    • /
    • 2006
  • The cDNA of a firefly luciferase from lantern mRNA of Lampyroidea maculata has been cloned, sequenced and functionally expressed. The cDNA has an open reading frame of 1647 bp and codes for a 548-residue-long polypeptide. Noteworthy, sequence comparison as well as homology modeling showed the highest degree of similarity with H. unmunsana and L. mingrelica luciferases, suggesting a close phylogenetic relationship despite the geographical distance separation. The deduced amino acid sequence of the luciferase gene of firefly L. maculata showed 93% identity to H. unmunsana. Superposition of the three-dimensional model of L. maculata luciferase (generated by homology modeling) and three dimensional structure of Photinus pyralis luciferase revealed that the spatial arrangements of Luciferin and ATP-binding residues are very similar. Putative signature of AMP-binding domain among the various firefly species and Lampyroidea maculata was compared and a striking similarity was found. Different motifs and sites have been identified in Lampyroidea maculata by sequence analysis. Expression and purification of luciferase from Lampyroidea maculata was carried out using Ni-NTA Sepharose. Bioluminescence emission spectrum was similar to Photinus pyralis luciferase.

Cloning and Expression of A Bacillus licheniformis Cellulase Gene (Bacillus licheniformis WL-12의 cellulase 유전자 클로닝과 발현)

  • Yoon, Ki-Hong
    • Korean Journal of Microbiology
    • /
    • v.42 no.4
    • /
    • pp.313-318
    • /
    • 2006
  • A thermophilic bacterium producing the extracellular cellulase was isolated from soybean paste, and the isolate WL-12 has been identified as Bacillus licheniformis on the basis on its 16S rRNA sequence, morphology and biochemical properties. A gene encoding the cellulase of B. licheniformis WL-12 was cloned and its nucleotide sequence was determined. This cellulase gene, designated celA, consisted of 1,551 nucleotides, encoding a polypeptide of 517 amino acid residues. The gene product contained catalytic domain and cellulose binding domain. The deduced amino acid sequence was highly homologous to those of cellulases of B. licheniformis, B. subtilis and B. amytoliquefaciens belonging to the glycosyl hydrolase family 5. When the celA gene was highly expressed using a strong B. subtilis promoter, the extracellular cellulase was produced up to 7.0 units/ml in B. subtilis WB700.

A Method and Tool for Identifying Domain Components Using Object Usage Information

  • Lee, Woo-Jin;Kwon, Oh-Cheon;Kim, Min-Jung;Shin, Gyu-Sang
    • ETRI Journal
    • /
    • v.25 no.2
    • /
    • pp.121-132
    • /
    • 2003
  • To enhance the productivity of software development and accelerate time to market, software developers have recently paid more attention to a component-based development (CBD) approach due to the benefits of component reuse. Among CBD processes, the identification of reusable components is a key but difficult process. Currently, component identification depends mainly on the intuition and experience of domain experts. In addition, there are few systematic methods or tools for component identification that enable domain experts to identify reusable components. This paper presents a systematic method and its tool called a component identifier that identifies software components by using object-oriented domain information, namely, use case models, domain object models, and sequence diagrams. To illustrate our method, we use the component identifier to identify candidates of reusable components from the object-oriented domain models of a banking system. The component identifier enables domain experts to easily identify reusable components by assisting and automating identification processes in an earlier development phase.

  • PDF

Output Voltage Ripple Analysis of Quantum Series Resonant Converter (QSRC의 출력전압맥동해석)

  • 임성운;권우현;조규형
    • Journal of the Korean Institute of Telematics and Electronics B
    • /
    • v.31B no.3
    • /
    • pp.141-149
    • /
    • 1994
  • In this paper, we could find optimum quantum sequence(OQS) to minimize the output ripple voltage of the quantum series resonant converter(QSRC). This sequence control is so general that it is irrelevant to the voltage gain so far as it is operating in the continuous conduction mode(CCM). Further more the dynamic range of QSRC is much extended by the optimum quantum sequence control(OQSC). Througuout the time-domain analysis, the solution of steady state and the boundary condition between continuous and discontinuous mode is QSRC is obtained. This feature is verified by simulations and experiments with good agreements.

  • PDF